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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of lactate to pyruvate.UniRule annotation

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation

Activity regulationi

Allosterically activated by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei16NAD; via amide nitrogenUniRule annotation1
Binding sitei37NADUniRule annotation1
Binding sitei42NADUniRule annotation1
Binding sitei68NADUniRule annotation1
Binding sitei85SubstrateUniRule annotation1
Binding sitei91SubstrateUniRule annotation1
Binding sitei104NADUniRule annotation1
Binding sitei146NADUniRule annotation1
Binding sitei156Allosteric activatorUniRule annotation1
Binding sitei171Allosteric activatorUniRule annotation1
Active sitei178Proton acceptorUniRule annotation1
Binding sitei231SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi82 – 83NADUniRule annotation2
Nucleotide bindingi121 – 123NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Oxidoreductase
LigandNAD

Enzyme and pathway databases

UniPathwayi
UPA00554;UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenaseUniRule annotation (EC:1.1.1.27UniRule annotation)
Short name:
L-LDHUniRule annotation
Gene namesi
Name:ldhUniRule annotation
Ordered Locus Names:CE2753
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000001409 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683391 – 317L-lactate dehydrogenaseAdd BLAST317

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei222PhosphotyrosineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ8FLV6

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi196164.HMPREF0290_0111

Structurei

3D structure databases

ProteinModelPortaliQ8FLV6
SMRiQ8FLV6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni123 – 126Substrate bindingUniRule annotation4
Regioni151 – 154Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
HOGENOMiHOG000213793
KOiK00016
OrthoDBiPOG091H02EQ

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

Q8FLV6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRFVGNRVVL IGAGDVGVAY AYALVNQGIA DELCIIDIDE KKLEGNVMDL
60 70 80 90 100
SHGVVWAGKR TKVRKGTYSD CEDAAMVVIC AGAAQKPGET RLQLVDKNVN
110 120 130 140 150
IMHTIVDEVM ANGFDGLFLV ATNPVDILTY AVWKFSGLDH SRVIGSGTVL
160 170 180 190 200
DTARFRYMLG ELYDVSPKSI HAYIIGEHGD TELPVLSSAT IAGVSMRKML
210 220 230 240 250
EKDPELEPRL EKIFEDTRDA AYRIIDAKGS TSYGIGMGLA RITRAILNNQ
260 270 280 290 300
DVALPVSAYL EGQYGEEDIY IGTPAIIDRS GIHRVVELEI SDREMSRFKH
310
SAQTLRAIKD EIFPVGE
Length:317
Mass (Da):34,784
Last modified:December 21, 2004 - v2
Checksum:iA2F12BEF117D9022
GO

Sequence cautioni

The sequence BAC19563 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA Translation: BAC19563.1 Different initiation.

Genome annotation databases

EnsemblBacteriaiBAC19563; BAC19563; BAC19563
KEGGicef:CE2753

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA Translation: BAC19563.1 Different initiation.

3D structure databases

ProteinModelPortaliQ8FLV6
SMRiQ8FLV6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196164.HMPREF0290_0111

Proteomic databases

PRIDEiQ8FLV6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC19563; BAC19563; BAC19563
KEGGicef:CE2753

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
HOGENOMiHOG000213793
KOiK00016
OrthoDBiPOG091H02EQ

Enzyme and pathway databases

UniPathwayi
UPA00554;UER00611

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLDH_COREF
AccessioniPrimary (citable) accession number: Q8FLV6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: December 21, 2004
Last modified: September 12, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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