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Protein

DNA ligase

Gene

ligA

Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.UniRule annotation

Catalytic activityi

NAD+ + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotide.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei113NADUniRule annotation1
Active sitei115N6-AMP-lysine intermediateUniRule annotation1
Binding sitei136NADUniRule annotation1
Binding sitei173NADUniRule annotation1
Binding sitei290NADUniRule annotation1
Binding sitei314NADUniRule annotation1
Metal bindingi408ZincUniRule annotation1
Metal bindingi411ZincUniRule annotation1
Metal bindingi426ZincUniRule annotation1
Metal bindingi432ZincUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi32 – 36NADUniRule annotation5
Nucleotide bindingi81 – 82NADUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Manganese, Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciECOL199310:C2945-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.2UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD(+)]UniRule annotation
Gene namesi
Name:ligAUniRule annotation
Ordered Locus Names:c2945
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000001410 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003132341 – 671DNA ligaseAdd BLAST671

Interactioni

Protein-protein interaction databases

STRINGi199310.c2945

Structurei

3D structure databases

ProteinModelPortaliQ8FFC1
SMRiQ8FFC1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini593 – 671BRCTUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C77 Bacteria
COG0272 LUCA
HOGENOMiHOG000218459
KOiK01972
OMAiFTAKSPR

Family and domain databases

CDDicd00027 BRCT, 1 hit
cd00114 LIGANc, 1 hit
Gene3Di3.40.50.10190, 1 hit
HAMAPiMF_01588 DNA_ligase_A, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR018239 DNA_ligase_AS
IPR033136 DNA_ligase_CS
IPR001679 DNAligase
IPR013839 DNAligase_adenylation
IPR013840 DNAligase_N
IPR003583 Hlx-hairpin-Hlx_DNA-bd_motif
IPR012340 NA-bd_OB-fold
IPR004150 NAD_DNA_ligase_OB
IPR010994 RuvA_2-like
IPR004149 Znf_DNAligase_C4
PfamiView protein in Pfam
PF00533 BRCT, 1 hit
PF01653 DNA_ligase_aden, 1 hit
PF03120 DNA_ligase_OB, 1 hit
PF03119 DNA_ligase_ZBD, 1 hit
PIRSFiPIRSF001604 LigA, 1 hit
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SM00278 HhH1, 4 hits
SM00532 LIGANc, 1 hit
SUPFAMiSSF47781 SSF47781, 1 hit
SSF50249 SSF50249, 1 hit
SSF52113 SSF52113, 1 hit
TIGRFAMsiTIGR00575 dnlj, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS01055 DNA_LIGASE_N1, 1 hit
PS01056 DNA_LIGASE_N2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8FFC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESIEQQLTE LRTTLRHHEY LYHVMDAPEI PDAEYDRLMR ELRELETKHP
60 70 80 90 100
ELITPDSPTQ RVGAAPLAAF SQIRHEVPML SLDNVFDEES FLAFNKRVQD
110 120 130 140 150
RLKSNEKVTW CCELKLDGLA VSILYENGVL VSAATRGDGT TGEDITSNVR
160 170 180 190 200
TIRAIPLKLH GENIPARLEV RGEVFLPQAG FEKINEDARR TGGKVFANPR
210 220 230 240 250
NAAAGSLRQL DPRITAKRPL TFFCYGVGVL EGGELPDTHL GRLLQFKKWG
260 270 280 290 300
LPVSDRVTLC ESAEEVLAFY HKVEEDRPTL GFDIDGVVIK VNSLAQQEQL
310 320 330 340 350
GFVARAPRWA VAFKFPAQEQ MTFVRDVEFQ VGRTGAITPV ARLEPVHVAG
360 370 380 390 400
VLVSNATLHN ADEIERLGLR IGDKVVIRRA GDVIPQVVNV VLSERPEDTR
410 420 430 440 450
EVVFPTHCPV CGSDVERVEG EAVARCTGGL ICGAQRKESL KHFVSRRAMD
460 470 480 490 500
VDGMGDKIID QLVEKEYVHT PADLFKLTAG KLTGLERMGP KSAQNVVNAL
510 520 530 540 550
EKAKETTFAR FLYALGIREV GEATAAGLAA YFGTLEALEA ASIEELQKVP
560 570 580 590 600
DVGIVVASHV HNFFAEESNR NVISELLAEG VHWPEPIVIN AEEIDSPFAG
610 620 630 640 650
KTVVLTGSLS QMSRDDAKAR LVELGAKVAG SVSKKTDLVI AGEAAGSKLA
660 670
KAQELGIEVI DETEMLRLLG S
Length:671
Mass (Da):73,667
Last modified:March 1, 2003 - v1
Checksum:i776C609E9A1B553A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014075 Genomic DNA Translation: AAN81395.1
RefSeqiWP_000443690.1, NC_004431.1

Genome annotation databases

EnsemblBacteriaiAAN81395; AAN81395; c2945
KEGGiecc:c2945

Similar proteinsi

Entry informationi

Entry nameiDNLJ_ECOL6
AccessioniPrimary (citable) accession number: Q8FFC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2003
Last modified: October 25, 2017
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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