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Entry version 107 (08 May 2019)
Sequence version 1 (01 Mar 2003)
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Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glutathione biosynthesis

This protein is involved in step 1 and 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutathione biosynthesis bifunctional protein GshAB (gshAB)
  2. Glutathione biosynthesis bifunctional protein GshAB (gshAB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi696Magnesium or manganese 1UniRule annotation1
Metal bindingi717Magnesium or manganese 1UniRule annotation1
Metal bindingi717Magnesium or manganese 2UniRule annotation1
Metal bindingi719Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi516 – 574ATPUniRule annotationAdd BLAST59

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLigase, Multifunctional enzyme
Biological processGlutathione biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
SMUT210007:G1FZX-281-MONOMER

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00142;UER00209
UPA00142;UER00210

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshABUniRule annotation
Alternative name(s):
Gamma-GCS-GSUniRule annotation
Short name:
GCS-GSUniRule annotation
Including the following 2 domains:
Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-ECSUniRule annotation
Short name:
GCSUniRule annotation
Gamma-glutamylcysteine synthetaseUniRule annotation
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:gshABUniRule annotation
Synonyms:gshFUniRule annotation
Ordered Locus Names:SMU_267c
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri210007 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002512 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001925601 – 754Glutathione biosynthesis bifunctional protein GshABAdd BLAST754

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
Q8DW15

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer.

UniRule annotation

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
210007.SMU_267c

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q8DW15

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini489 – 752ATP-graspUniRule annotationAdd BLAST264

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 333Glutamate--cysteine ligaseAdd BLAST333

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105D9M Bacteria
COG1181 LUCA
COG2918 LUCA

KEGG Orthology (KO)

More...
KOi
K01919

Identification of Orthologs from Complete Genome Data

More...
OMAi
EANFNPM

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q8DW15

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.1490.20, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_00782 Glut_biosynth, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR014746 Gln_synth/guanido_kin_cat_dom
IPR007370 Glu_cys_ligase
IPR006335 Glut_biosynth
IPR006334 Glut_cys_ligase
IPR040657 GshAB_ATP-grasp
IPR020561 PRibGlycinamid_synth_ATP-grasp

The PANTHER Classification System

More...
PANTHERi
PTHR38761 PTHR38761, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF18419 ATP-grasp_6, 1 hit
PF01071 GARS_A, 1 hit
PF04262 Glu_cys_ligase, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF55931 SSF55931, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01435 glu_cys_lig_rel, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50975 ATP_GRASP, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q8DW15-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHINQLLQHA NSDLPLLQAN FGLERESLRI NKTNHRLAQT PHPTALGSRQ
60 70 80 90 100
FHPYIQTDYS ESQMELITPV AHSSKEVLRF LGAITDVAER SIDQNQYLWP
110 120 130 140 150
LSMPPQITED EIEIAQLEDD FEFSYRQYLD KKYGKILQSI SGIHYNMELG
160 170 180 190 200
ADLMNELFEL SGYQSFIDFK NDLYLKVAQN FLNYRWFLTY LYGASPLAEK
210 220 230 240 250
GFLNEELSQT VRSIRNSHLG YVNTDDIKVP FDSLENYISS IEHYVKSGAL
260 270 280 290 300
SAEKEFYSAV RLRGSKHNRD YLTKGITYLE FRCFDLNPFN NRGITQETID
310 320 330 340 350
SVHLFILAML WLDTPKKLNQ ALDKAQKLND KIALSHPLEK LPKENSASLI
360 370 380 390 400
IEAMEALIKH FKLPSYYDDL LIAIKKQVEN PKLTLSGRLF EHIKHASLEH
410 420 430 440 450
FGQKKGQDYH NYAWQNYYAL KGYENMELST QMLLFDTIQK GIHFEILDEN
460 470 480 490 500
DQFLKLWHND HIEYVKNGNM TSKDNYVIPL AMANKVVTKK ILRENGYPVP
510 520 530 540 550
AGAEFDNKDE ALRYYSQIKN KPIVVKPKTT NFGLGISIFE TAASHNDYEK
560 570 580 590 600
ALDIAFIEDY SVLVEEFIPG TEYRFFILDG KCEAVLLRVA ANVVGDGHST
610 620 630 640 650
VRQLVAQKNR DPLRGREHRS PLEIIDLGDI ELLMLQQEGY TLEDILPKGK
660 670 680 690 700
KVNLRGNSNI STGGDSIDVT ETMDPSYKQL AANMATAMGA WVCGVDLIIP
710 720 730 740 750
DTNLKASKGK PNCTCIELNF NPSMYMHTYC YQGPGQVITG KILAKLFPEI

STKI
Length:754
Mass (Da):86,187
Last modified:March 1, 2003 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i103479A8536A10CB
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AE014133 Genomic DNA Translation: AAN58035.1

NCBI Reference Sequences

More...
RefSeqi
NP_720729.1, NC_004350.2
WP_002262729.1, NC_004350.2

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAN58035; AAN58035; SMU_267c

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
1027852

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
smu:SMU_267c

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|210007.7.peg.233

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014133 Genomic DNA Translation: AAN58035.1
RefSeqiNP_720729.1, NC_004350.2
WP_002262729.1, NC_004350.2

3D structure databases

SMRiQ8DW15
ModBaseiSearch...

Protein-protein interaction databases

STRINGi210007.SMU_267c

Proteomic databases

PRIDEiQ8DW15

Genome annotation databases

EnsemblBacteriaiAAN58035; AAN58035; SMU_267c
GeneIDi1027852
KEGGismu:SMU_267c
PATRICifig|210007.7.peg.233

Phylogenomic databases

eggNOGiENOG4105D9M Bacteria
COG1181 LUCA
COG2918 LUCA
KOiK01919
OMAiEANFNPM
PhylomeDBiQ8DW15

Enzyme and pathway databases

UniPathwayiUPA00142;UER00209
UPA00142;UER00210
BioCyciSMUT210007:G1FZX-281-MONOMER

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00782 Glut_biosynth, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR014746 Gln_synth/guanido_kin_cat_dom
IPR007370 Glu_cys_ligase
IPR006335 Glut_biosynth
IPR006334 Glut_cys_ligase
IPR040657 GshAB_ATP-grasp
IPR020561 PRibGlycinamid_synth_ATP-grasp
PANTHERiPTHR38761 PTHR38761, 1 hit
PfamiView protein in Pfam
PF18419 ATP-grasp_6, 1 hit
PF01071 GARS_A, 1 hit
PF04262 Glu_cys_ligase, 1 hit
SUPFAMiSSF55931 SSF55931, 1 hit
TIGRFAMsiTIGR01435 glu_cys_lig_rel, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiGSHAB_STRMU
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8DW15
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2003
Last modified: May 8, 2019
This is version 107 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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