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UniProtKB - Q8DR59 (PBPA_STRR6)
Protein
Penicillin-binding protein 1A
Gene
pbpA
Organism
Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
Status
Functioni
Cell wall formation.
Catalytic activityi
- [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H+By similarityEC:2.4.1.129By similarity
- Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.By similarity EC:3.4.16.4
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 91 | Proton donor; for transglycosylase activityBy similarity | 1 | |
Active sitei | 370 | Acyl-ester intermediate; for transpeptidase activityBy similarity | 1 |
GO - Molecular functioni
- penicillin binding Source: GO_Central
- peptidoglycan glycosyltransferase activity Source: GO_Central
- serine-type D-Ala-D-Ala carboxypeptidase activity Source: UniProtKB-EC
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: GO_Central
- regulation of cell shape Source: UniProtKB-KW
- response to antibiotic Source: GO_Central
Keywordsi
Molecular function | Carboxypeptidase, Glycosyltransferase, Hydrolase, Multifunctional enzyme, Protease, Transferase |
Biological process | Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
Protein family/group databases
CAZyi | GT51, Glycosyltransferase Family 51 |
Names & Taxonomyi
Protein namesi | Recommended name: Penicillin-binding protein 1AShort name: PBP-1A Alternative name(s): Exported protein 2 Including the following 2 domains: |
Gene namesi | Name:pbpA Synonyms:exp21 Publication, ponA1 Publication Ordered Locus Names:spr0329 |
Organismi | Streptococcus pneumoniae (strain ATCC BAA-255 / R6) |
Taxonomic identifieri | 171101 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Disruption phenotypei
Not essential, it can be deleted.1 Publication
Chemistry databases
DrugBanki | DB08375, (2R)-2-[(1R)-1-[[(2Z)-2-(2-Amino-1,3-thiazol-4-yl)-2-methoxyiminoacetyl]amino]-2-oxoethyl]-5-methylidene-2H-1,3-thiazine-4-carboxylic acid DB01163, Amdinocillin DB00415, Ampicillin DB08795, Azidocillin DB01140, Cefadroxil DB00456, Cefalotin DB00493, Cefotaxime DB01331, Cefoxitin DB00567, Cephalexin DB03313, Cephalosporin C DB01147, Cloxacillin DB01000, Cyclacillin DB00485, Dicloxacillin DB00739, Hetacillin DB01603, Meticillin DB00607, Nafcillin DB00713, Oxacillin |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000083183 | 1 – 719 | Penicillin-binding protein 1AAdd BLAST | 719 |
Proteomic databases
PRIDEi | Q8DR59 |
Interactioni
Subunit structurei
Interacts with MreC in the elongasome.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4182173, 1 interactor |
STRINGi | 171101.spr0329 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q8DR59 |
SMRi | Q8DR59 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8DR59 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 62 – 223 | TransglycosylaseBy similarityAdd BLAST | 162 | |
Regioni | 297 – 611 | TranspeptidaseBy similarityAdd BLAST | 315 | |
Regioni | 652 – 719 | DisorderedSequence analysisAdd BLAST | 68 |
Sequence similaritiesi
In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated
Phylogenomic databases
eggNOGi | COG0744, Bacteria |
HOGENOMi | CLU_006354_2_5_9 |
OMAi | LAQMAMI |
Family and domain databases
Gene3Di | 1.10.3810.10, 1 hit 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR001264, Glyco_trans_51 IPR023346, Lysozyme-like_dom_sf IPR036950, PBP_transglycosylase IPR001460, PCN-bd_Tpept |
Pfami | View protein in Pfam PF00912, Transgly, 1 hit PF00905, Transpeptidase, 1 hit |
SUPFAMi | SSF53955, SSF53955, 1 hit SSF56601, SSF56601, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8DR59-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNKPTILRLI KYLSISFLSL VIAAIVLGGG VFFYYVSKAP SLSESKLVAT
60 70 80 90 100
TSSKIYDNKN QLIADLGSER RVNAQANDIP TDLVKAIVSI EDHRFFDHRG
110 120 130 140 150
IDTIRILGAF LRNLQSNSLQ GGSALTQQLI KLTYFSTSTS DQTISRKAQE
160 170 180 190 200
AWLAIQLEQK ATKQEILTYY INKVYMSNGN YGMQTAAQNY YGKDLNNLSL
210 220 230 240 250
PQLALLAGMP QAPNQYDPYS HPEAAQDRRN LVLSEMKNQG YISAEQYEKA
260 270 280 290 300
VNTPITDGLQ SLKSASNYPA YMDNYLKEVI NQVEEETGYN LLTTGMDVYT
310 320 330 340 350
NVDQEAQKHL WDIYNTDEYV AYPDDELQVA STIVDVSNGK VIAQLGARHQ
360 370 380 390 400
SSNVSFGINQ AVETNRDWGS TMKPITDYAP ALEYGVYEST ATIVHDEPYN
410 420 430 440 450
YPGTNTPVYN WDRGYFGNIT LQYALQQSRN VPAVETLNKV GLNRAKTFLN
460 470 480 490 500
GLGIDYPSIH YSNAISSNTT ESDKKYGASS EKMAAAYAAF ANGGTYYKPM
510 520 530 540 550
YIHKVVFSDG SEKEFSNVGT RAMKETTAYM MTDMMKTVLS YGTGRNAYLA
560 570 580 590 600
WLPQAGKTGT SNYTDEEIEN HIKTSQFVAP DELFAGYTRK YSMAVWTGYS
610 620 630 640 650
NRLTPLVGNG LTVAAKVYRS MMTYLSEGSN PEDWNIPEGL YRNGEFVFKN
660 670 680 690 700
GARSTWSSPA PQQPPSTESS SSSSDSSTSQ SSSTTPSTNN STTTNPNNNT
710
QQSNTTPDQQ NQNPQPAQP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M90527 Genomic DNA Translation: AAA26956.1 AE007317 Genomic DNA Translation: AAK99133.1 |
PIRi | A42893 A97913 |
RefSeqi | NP_357923.1, NC_003098.1 WP_001039991.1, NC_003098.1 |
Genome annotation databases
EnsemblBacteriai | AAK99133; AAK99133; spr0329 |
GeneIDi | 60234246 |
KEGGi | spr:spr0329 |
PATRICi | fig|171101.6.peg.368 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M90527 Genomic DNA Translation: AAA26956.1 AE007317 Genomic DNA Translation: AAK99133.1 |
PIRi | A42893 A97913 |
RefSeqi | NP_357923.1, NC_003098.1 WP_001039991.1, NC_003098.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2C5W | X-ray | 2.55 | A | 51-66 | [»] | |
B | 266-650 | [»] | ||||
2C6W | X-ray | 2.61 | A | 51-66 | [»] | |
B | 267-650 | [»] | ||||
2V2F | X-ray | 1.90 | A | 47-70 | [»] | |
2ZC5 | X-ray | 3.00 | A/C | 47-70 | [»] | |
2ZC6 | X-ray | 2.70 | A/C | 47-70 | [»] | |
AlphaFoldDBi | Q8DR59 | |||||
SMRi | Q8DR59 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4182173, 1 interactor |
STRINGi | 171101.spr0329 |
Chemistry databases
DrugBanki | DB08375, (2R)-2-[(1R)-1-[[(2Z)-2-(2-Amino-1,3-thiazol-4-yl)-2-methoxyiminoacetyl]amino]-2-oxoethyl]-5-methylidene-2H-1,3-thiazine-4-carboxylic acid DB01163, Amdinocillin DB00415, Ampicillin DB08795, Azidocillin DB01140, Cefadroxil DB00456, Cefalotin DB00493, Cefotaxime DB01331, Cefoxitin DB00567, Cephalexin DB03313, Cephalosporin C DB01147, Cloxacillin DB01000, Cyclacillin DB00485, Dicloxacillin DB00739, Hetacillin DB01603, Meticillin DB00607, Nafcillin DB00713, Oxacillin |
Protein family/group databases
CAZyi | GT51, Glycosyltransferase Family 51 |
Proteomic databases
PRIDEi | Q8DR59 |
Genome annotation databases
EnsemblBacteriai | AAK99133; AAK99133; spr0329 |
GeneIDi | 60234246 |
KEGGi | spr:spr0329 |
PATRICi | fig|171101.6.peg.368 |
Phylogenomic databases
eggNOGi | COG0744, Bacteria |
HOGENOMi | CLU_006354_2_5_9 |
OMAi | LAQMAMI |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
Miscellaneous databases
EvolutionaryTracei | Q8DR59 |
Family and domain databases
Gene3Di | 1.10.3810.10, 1 hit 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR001264, Glyco_trans_51 IPR023346, Lysozyme-like_dom_sf IPR036950, PBP_transglycosylase IPR001460, PCN-bd_Tpept |
Pfami | View protein in Pfam PF00912, Transgly, 1 hit PF00905, Transpeptidase, 1 hit |
SUPFAMi | SSF53955, SSF53955, 1 hit SSF56601, SSF56601, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PBPA_STRR6 | |
Accessioni | Q8DR59Primary (citable) accession number: Q8DR59 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2003 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families