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Protein

Bifunctional NAD(P)H-hydrate repair enzyme Nnr

Gene

nnr

Organism
Thermosynechococcus elongatus (strain BP-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).By similarity

Catalytic activityi

ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide = AMP + phosphate + NADH.
ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide phosphate = AMP + phosphate + NADPH.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+By similarityNote: Binds 1 potassium ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi64PotassiumBy similarity1
Metal bindingi124PotassiumBy similarity1
Binding sitei157NAD(P)HX (for epimerase activity)By similarity1
Metal bindingi160PotassiumBy similarity1
Binding sitei330NAD(P)HX (for dehydratase activity); via amide nitrogenBy similarity1
Binding sitei442NAD(P)HX (for dehydratase activity)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi412 – 416ADPBy similarity5
Nucleotide bindingi432 – 441ADPBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme
LigandATP-binding, Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciTELO197221:G1G3I-2442-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional NAD(P)H-hydrate repair enzyme Nnr
Alternative name(s):
Nicotinamide nucleotide repair protein
Including the following 2 domains:
ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC:4.2.1.136)
Alternative name(s):
ADP-dependent NAD(P)HX dehydratase
NAD(P)H-hydrate epimerase (EC:5.1.99.6)
Alternative name(s):
NAD(P)HX epimerase
Gene namesi
Name:nnr
Ordered Locus Names:tll2395
OrganismiThermosynechococcus elongatus (strain BP-1)
Taxonomic identifieri197221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeThermosynechococcus
Proteomesi
  • UP000000440 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004164231 – 505Bifunctional NAD(P)H-hydrate repair enzyme NnrAdd BLAST505

Interactioni

Protein-protein interaction databases

STRINGi197221.tll2395

Structurei

3D structure databases

ProteinModelPortaliQ8DGC3
SMRiQ8DGC3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 214YjeF N-terminalAdd BLAST201
Domaini226 – 500YjeF C-terminalAdd BLAST275

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 219NAD(P)H-hydrate epimeraseBy similarityAdd BLAST219
Regioni63 – 67NAD(P)HX (for epimerase activity)By similarity5
Regioni128 – 134NAD(P)HX (for epimerase activity)By similarity7
Regioni227 – 505ADP-dependent (S)-NAD(P)H-hydrate dehydrataseBy similarityAdd BLAST279
Regioni376 – 382NAD(P)HX (for dehydratase activity)By similarity7

Sequence similaritiesi

In the N-terminal section; belongs to the NnrE/AIBP family.Curated
In the C-terminal section; belongs to the NnrD/CARKD family.Curated

Phylogenomic databases

eggNOGiENOG4105DR1 Bacteria
COG0062 LUCA
COG0063 LUCA
HOGENOMiHOG000228404
KOiK17758
K17759
OMAiLVGPGHN
OrthoDBiPOG091H01XZ

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.10260, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
MF_01966 NADHX_epimerase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR030677 Nnr
IPR029056 Ribokinase-like
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
PF03853 YjeF_N, 1 hit
PIRSFiPIRSF017184 Nnr, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
SSF64153 SSF64153, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
TIGR00197 yjeF_nterm, 1 hit
PROSITEiView protein in PROSITE
PS01050 YJEF_C_2, 1 hit
PS51383 YJEF_C_3, 1 hit
PS51385 YJEF_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q8DGC3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKPTFPAIVT TAEMQAIEGA MFNGGLPIPA LMEKVGQRLS HYLQAHFPTS
60 70 80 90 100
QYPRVAVLAG PGHNGGDALV VARELWHRGY QVKLWQPFER LKPLTADHAR
110 120 130 140 150
YARFLGLPFV ERVEALQEVD VIVDGLFGFG LERELTGELA HAIDEINTWP
160 170 180 190 200
QPRVSIDVPS GLHSDTGAVL GTAIRADRTL CLGLWKRGLL VEEAQPWVGQ
210 220 230 240 250
GVLIPFDIPS VVIETALASA PRRYCLDDSC WQALPLSRSP ITHKYQQGQL
260 270 280 290 300
LLIGGSGQFG GSILLSALAA RCTGVGMLVV AVPQSLKSLV LSRVPDAIVV
310 320 330 340 350
GCPETPRGAI ARLPEGLELG KFSAIACGPG LTPEAVSVVA TVLRAETSLV
360 370 380 390 400
LDADALNILA TLSPWPLPSG TILTPHYGEF RRLFPDLVGT AGDRLDQVIA
410 420 430 440 450
AARWSNAIVL LKGARTAIAS ARGDLWINPH STPALARGGS GDVLTGLIGG
460 470 480 490 500
LLAQQEALRA TYGGVWWHAQ AALEAEQQAT SLGVYPEQLI AHLLPTLRRA

LAARV
Length:505
Mass (Da):53,934
Last modified:March 1, 2003 - v1
Checksum:i503588EDBFF6BCF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000039 Genomic DNA Translation: BAC09947.1
RefSeqiNP_683185.1, NC_004113.1
WP_011058227.1, NC_004113.1

Genome annotation databases

EnsemblBacteriaiBAC09947; BAC09947; BAC09947
GeneIDi1012560
KEGGitel:tll2395
PATRICifig|197221.4.peg.2516

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000039 Genomic DNA Translation: BAC09947.1
RefSeqiNP_683185.1, NC_004113.1
WP_011058227.1, NC_004113.1

3D structure databases

ProteinModelPortaliQ8DGC3
SMRiQ8DGC3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi197221.tll2395

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC09947; BAC09947; BAC09947
GeneIDi1012560
KEGGitel:tll2395
PATRICifig|197221.4.peg.2516

Phylogenomic databases

eggNOGiENOG4105DR1 Bacteria
COG0062 LUCA
COG0063 LUCA
HOGENOMiHOG000228404
KOiK17758
K17759
OMAiLVGPGHN
OrthoDBiPOG091H01XZ

Enzyme and pathway databases

BioCyciTELO197221:G1G3I-2442-MONOMER

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.10260, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
MF_01966 NADHX_epimerase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR030677 Nnr
IPR029056 Ribokinase-like
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
PF03853 YjeF_N, 1 hit
PIRSFiPIRSF017184 Nnr, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
SSF64153 SSF64153, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
TIGR00197 yjeF_nterm, 1 hit
PROSITEiView protein in PROSITE
PS01050 YJEF_C_2, 1 hit
PS51383 YJEF_C_3, 1 hit
PS51385 YJEF_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNNR_THEEB
AccessioniPrimary (citable) accession number: Q8DGC3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: March 1, 2003
Last modified: October 10, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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