thyA

Functionⁱ

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityⁱ

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayⁱ: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	22	dUMPUniRule annotation	1
Active siteⁱ	160	NucleophileUniRule annotation	1
Binding siteⁱ	183	5,10-methylenetetrahydrofolateUniRule annotation	1
Binding siteⁱ	191	dUMPUniRule annotation	1
Binding siteⁱ	282	5,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	180 – 183	dUMPUniRule annotation		4
Nucleotide bindingⁱ	221 – 223	dUMPUniRule annotation		3

GO - Molecular functionⁱ

thymidylate synthase activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

dTMP biosynthetic process Source: InterPro
dTTP biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Methyltransferase, Transferase
Biological process	Nucleotide biosynthesis

Enzyme and pathway databases

BioCycⁱ	VVUL216895:G1GJ4-505-MONOMER.
UniPathwayⁱ	UPA00575.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation) Short name: TSUniRule annotation Short name: TSaseUniRule annotation
Gene namesⁱ	Name:thyAUniRule annotation Ordered Locus Names:VV1_0516
Organismⁱ	Vibrio vulnificus (strain CMCP6)
Taxonomic identifierⁱ	216895 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio vulnificus

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00008

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000141042	1 – 283	Thymidylate synthaseAdd BLAST		283

Interactionⁱ

Subunit structureⁱ

Homodimer.UniRule annotation

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8DER9.
SMRⁱ	Q8DER9.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105C0V. Bacteria. COG0207. LUCA.
HOGENOMⁱ	HOG000257900.
KEGG Orthology (KO) More...KOⁱ	K00560.
OMAⁱ	KQYLDLC.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H02L6.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Dⁱ	3.30.572.10. 1 hit.
HAMAPⁱ	MF_00008. Thymidy_synth_bact. 1 hit.
InterProⁱ	View protein in InterPro IPR023451. Thymidate_synth/dCMP_Mease. IPR036926. Thymidate_synth/dCMP_Mease_sf. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00303. Thymidylat_synt. 1 hit.
PRINTSⁱ	PR00108. THYMDSNTHASE.
SUPFAMⁱ	SSF55831. SSF55831. 1 hit.
TIGRFAMsⁱ	TIGR03284. thym_sym. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00091. THYMIDYLATE_SYNTHASE. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q8DER9-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKQYLELCRR IVDEGHWVEN ERTGKRCLTV INADLTYDVA NNQFPLVTTR 
        60         70         80         90        100
KSFWKAAVAE LLGYIRGYDN AEDFRKLGTK TWDANANLND AWLNNPYRKG 
       110        120        130        140        150
EDDMGRVYGV QGRAWAKPDG GHIDQLRKIV DDLTRGVDDR GEILNFYNPG 
       160        170        180        190        200
EFHMGCLRPC MYSHHFSLLG DTLYLNSTQR SCDVPLGLNF NMVQVYVFLA 
       210        220        230        240        250
IMAQITGKKP GQAFHKIVNA HIYEDQLALM RDVQLKREPL QAPTFHINPE 
       260        270        280 
IKSLEDLETW VTLDDFWVEG YEHHDPIQYP FSV

Length:283

Mass (Da):32,717

Last modified:March 1, 2003 - v1

Checksum:ⁱF4623752B1B3F97A

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE016795 Genomic DNA. Translation: AAO09034.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011078604.1. NC_004459.3.

Genome annotation databases

EnsemblBacteriaⁱ	AAO09034; AAO09034; VV1_0516.
KEGGⁱ	vvu:VV1_0516.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q8DER9	A0A1V8N0H8 UPI0002D76F85 UPI0009B84011 UPI000576D58C	Vibrio vulnificus	283	UniRef100_Q8DER9	Cluster: Thymidylate synthase	5

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q8DER9	O66108 C3LST0 A0A0H6NUW5 A0A0H3QAH6 D7HD57 A0A0K9UUG6 A0A0X1L3L6 A0A0H6QST8 A0A0H6JJ38 A0A0H5ZZM5 +176	Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) Vibrio cholerae serotype O1 (strain M66-2) Vibrio cholerae Vibrio cholerae B33 Vibrio cholerae RC385 Vibrio cholerae 2740-80 Vibrio cholerae (strain MO10) Vibrio sp. JCM 18905 Vibrio antiquarius (strain Ex25) Vibrio parahaemolyticus And more	283	UniRef90_O66108	Cluster: Thymidylate synthase	187

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q8DER9	O66108 C3LST0 A0A0H6NUW5 A0A0H3QAH6 D7HD57 A0A0K9UUG6 A0A0X1L3L6 A0A0H6QST8 A0A0H6JJ38 A0A0H5ZZM5 +1065	Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) Vibrio cholerae serotype O1 (strain M66-2) Vibrio cholerae Vibrio cholerae B33 Vibrio cholerae RC385 Vibrio cholerae 2740-80 Vibrio cholerae (strain MO10) Vibrio metoecus Vibrio sp. JCM 18905 Vibrio antiquarius (strain Ex25) And more	283	UniRef50_O66108	Cluster: Thymidylate synthase	1,076

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE016795 Genomic DNA. Translation: AAO09034.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011078604.1. NC_004459.3.

3D structure databases

ProteinModelPortalⁱ	Q8DER9.
SMRⁱ	Q8DER9.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAO09034; AAO09034; VV1_0516.
KEGGⁱ	vvu:VV1_0516.

Phylogenomic databases

eggNOGⁱ	ENOG4105C0V. Bacteria. COG0207. LUCA.
HOGENOMⁱ	HOG000257900.
KEGG Orthology (KO) More...KOⁱ	K00560.
OMAⁱ	KQYLDLC.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H02L6.

Enzyme and pathway databases

UniPathwayⁱ	UPA00575.
BioCycⁱ	VVUL216895:G1GJ4-505-MONOMER.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Dⁱ	3.30.572.10. 1 hit.
HAMAPⁱ	MF_00008. Thymidy_synth_bact. 1 hit.
InterProⁱ	View protein in InterPro IPR023451. Thymidate_synth/dCMP_Mease. IPR036926. Thymidate_synth/dCMP_Mease_sf. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00303. Thymidylat_synt. 1 hit.
PRINTSⁱ	PR00108. THYMDSNTHASE.
SUPFAMⁱ	SSF55831. SSF55831. 1 hit.
TIGRFAMsⁱ	TIGR03284. thym_sym. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TYSY_VIBVU
Accessionⁱ	Q8DER9Primary (citable) accession number: Q8DER9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 4, 2003
	Last sequence update:	March 1, 2003
	Last modified:	February 28, 2018
	This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8DER9 (TYSY_VIBVU)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Thymidylate synthase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: dTTP biosynthesis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

Your basket is currently empty. ⁱ

Functionⁱ

Pathwayⁱ: dTTP biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ