UniProtKB - Q8DER9 (TYSY_VIBVU)
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Protein
Thymidylate synthase
Gene
thyA
Organism
Vibrio vulnificus (strain CMCP6)
Status
Functioni
Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation
Catalytic activityi
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation
: dTTP biosynthesis Pathwayi
This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationView all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 22 | dUMPUniRule annotation | 1 | |
Active sitei | 160 | NucleophileUniRule annotation | 1 | |
Binding sitei | 183 | 5,10-methylenetetrahydrofolateUniRule annotation | 1 | |
Binding sitei | 191 | dUMPUniRule annotation | 1 | |
Binding sitei | 282 | 5,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 180 – 183 | dUMPUniRule annotation | 4 | |
Nucleotide bindingi | 221 – 223 | dUMPUniRule annotation | 3 |
GO - Molecular functioni
- thymidylate synthase activity Source: UniProtKB-EC
GO - Biological processi
- dTMP biosynthetic process Source: InterPro
- dTTP biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Nucleotide biosynthesis |
Enzyme and pathway databases
BioCyci | VVUL216895:G1GJ4-505-MONOMER. |
UniPathwayi | UPA00575. |
Names & Taxonomyi
Protein namesi | Recommended name: Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)Short name: TSUniRule annotation Short name: TSaseUniRule annotation |
Gene namesi | Name:thyAUniRule annotation Ordered Locus Names:VV1_0516 |
Organismi | Vibrio vulnificus (strain CMCP6) |
Taxonomic identifieri | 216895 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › |
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000141042 | 1 – 283 | Thymidylate synthaseAdd BLAST | 283 |
Interactioni
Subunit structurei
Homodimer.UniRule annotation
Structurei
3D structure databases
ProteinModelPortali | Q8DER9. |
SMRi | Q8DER9. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4105C0V. Bacteria. COG0207. LUCA. |
HOGENOMi | HOG000257900. |
KOi | K00560. |
OMAi | KQYLDLC. |
OrthoDBi | POG091H02L6. |
Family and domain databases
CDDi | cd00351. TS_Pyrimidine_HMase. 1 hit. |
Gene3Di | 3.30.572.10. 1 hit. |
HAMAPi | MF_00008. Thymidy_synth_bact. 1 hit. |
InterProi | View protein in InterPro IPR023451. Thymidate_synth/dCMP_Mease. IPR036926. Thymidate_synth/dCMP_Mease_sf. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. |
Pfami | View protein in Pfam PF00303. Thymidylat_synt. 1 hit. |
PRINTSi | PR00108. THYMDSNTHASE. |
SUPFAMi | SSF55831. SSF55831. 1 hit. |
TIGRFAMsi | TIGR03284. thym_sym. 1 hit. |
PROSITEi | View protein in PROSITE PS00091. THYMIDYLATE_SYNTHASE. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q8DER9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKQYLELCRR IVDEGHWVEN ERTGKRCLTV INADLTYDVA NNQFPLVTTR
60 70 80 90 100
KSFWKAAVAE LLGYIRGYDN AEDFRKLGTK TWDANANLND AWLNNPYRKG
110 120 130 140 150
EDDMGRVYGV QGRAWAKPDG GHIDQLRKIV DDLTRGVDDR GEILNFYNPG
160 170 180 190 200
EFHMGCLRPC MYSHHFSLLG DTLYLNSTQR SCDVPLGLNF NMVQVYVFLA
210 220 230 240 250
IMAQITGKKP GQAFHKIVNA HIYEDQLALM RDVQLKREPL QAPTFHINPE
260 270 280
IKSLEDLETW VTLDDFWVEG YEHHDPIQYP FSV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE016795 Genomic DNA. Translation: AAO09034.1. |
RefSeqi | WP_011078604.1. NC_004459.3. |
Genome annotation databases
EnsemblBacteriai | AAO09034; AAO09034; VV1_0516. |
KEGGi | vvu:VV1_0516. |
Similar proteinsi
Entry informationi
Entry namei | TYSY_VIBVU | |
Accessioni | Q8DER9Primary (citable) accession number: Q8DER9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 4, 2003 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 28, 2018 | |
This is version 96 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |