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Protein

Glutamate--cysteine ligase

Gene

gshA

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation

Pathwayi: glutathione biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase (gshA)
  2. Glutathione synthetase (gshB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processGlutathione biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00209

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-ECSUniRule annotation
Short name:
GCSUniRule annotation
Gamma-glutamylcysteine synthetaseUniRule annotation
Gene namesi
Name:gshAUniRule annotation
Ordered Locus Names:VV1_1606
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001925441 – 525Glutamate--cysteine ligaseAdd BLAST525

Structurei

3D structure databases

ProteinModelPortaliQ8DC38
SMRiQ8DC38
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D9M Bacteria
COG2918 LUCA
HOGENOMiHOG000266224
KOiK01919
OMAiRYSWLLM
OrthoDBiPOG091H0622

Family and domain databases

HAMAPiMF_00578 Glu_cys_ligase, 1 hit
InterProiView protein in InterPro
IPR014746 Gln_synth/guanido_kin_cat_dom
IPR007370 Glu_cys_ligase
IPR006334 Glut_cys_ligase
PANTHERiPTHR38761 PTHR38761, 1 hit
PfamiView protein in Pfam
PF04262 Glu_cys_ligase, 1 hit
SUPFAMiSSF55931 SSF55931, 1 hit
TIGRFAMsiTIGR01434 glu_cys_ligase, 1 hit

Sequencei

Sequence statusi: Complete.

Q8DC38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFILTDFAA RLELVARNPE VFKQFGRGVE RETLRYSQNG RIATTMHPQG
60 70 80 90 100
LGSAFTNQWI TTDFAESLLE FITPVSHDID VLLGQLDDIH HFTQTQLGEE
110 120 130 140 150
KMWPMSMPCY VETEDQITLA QYGSSNSAKM KTLYREGLKR RYGSLMQIIS
160 170 180 190 200
GVHFNFSFPE SFWDALHGEQ TEDERQATKS EAYFGLIRNY YRFGWLIPYF
210 220 230 240 250
FGASPAMCSS FLQGRETSLP FEALGKTLYL PKATSLRLSD LGYTNSAQSV
260 270 280 290 300
LTIGFNSIDE YLEGLTKAIR TPSAEFAKLG VKENGEYRQL NSNVLQIENE
310 320 330 340 350
LYAPIRPKRV AKNGEKPSEA LARGGVEYIE VRSLDVNPFT PVGITETQVR
360 370 380 390 400
FLDLFLTWAA LSESQPMDQC ELACWRENWN KVVVSGREYG LELQIGCKGE
410 420 430 440 450
KLSLQAWAHR VFAELRQLAE VMDSAHGDNQ YSLACSELEQ WIDHPEKTLS
460 470 480 490 500
AQLLTLIQQN GSLGATGCEL GGAYREQNLA HHYRHFSLQQ MEQEVALSLI
510 520
KQSQIEQADE VDFDTYLADY FAYLK
Length:525
Mass (Da):59,632
Last modified:March 1, 2003 - v1
Checksum:i1D122E70213FCFE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA Translation: AAO10027.1

Genome annotation databases

EnsemblBacteriaiAAO10027; AAO10027; VV1_1606
KEGGivvu:VV1_1606

Similar proteinsi

Entry informationi

Entry nameiGSH1_VIBVU
AccessioniPrimary (citable) accession number: Q8DC38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: March 1, 2003
Last modified: December 20, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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