thyA

Functionⁱ

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityⁱ

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayⁱ: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	21	dUMPUniRule annotation	1
Binding siteⁱ	51	5,10-methylenetetrahydrofolateUniRule annotation	1
Active siteⁱ	146	NucleophileUniRule annotation	1
Binding siteⁱ	169	5,10-methylenetetrahydrofolateUniRule annotation	1
Binding siteⁱ	177	dUMPUniRule annotation	1
Binding siteⁱ	263	5,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	126 – 127	dUMP; shared with dimeric partnerUniRule annotation	2
Nucleotide bindingⁱ	166 – 169	dUMPUniRule annotation	4
Nucleotide bindingⁱ	207 – 209	dUMPUniRule annotation	3

GO - Molecular functionⁱ

thymidylate synthase activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

dTMP biosynthetic process Source: InterPro
dTTP biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Methyltransferase, Transferase
Biological process	Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayⁱ	UPA00575.

UniPathwayⁱ

UPA00575.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation) Short name: TSUniRule annotation Short name: TSaseUniRule annotation
Gene namesⁱ	Name:thyAUniRule annotation Ordered Locus Names:WIGBR3200
Organismⁱ	Wigglesworthia glossinidia brevipalpis
Taxonomic identifierⁱ	36870 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Erwiniaceae › Wigglesworthia › Wigglesworthia glossinidia
Proteomesⁱ	UP000000562 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00008

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000141044	1 – 264	Thymidylate synthaseAdd BLAST		264

Interactionⁱ

Subunit structureⁱ

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGⁱ	36870.WGLp320.

STRINGⁱ

36870.WGLp320.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8D2N4.
SMRⁱ	Q8D2N4.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105C0V. Bacteria. COG0207. LUCA.
KEGG Orthology (KO) More...KOⁱ	K00560.
OMAⁱ	KQYLDLC.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H02L6.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Dⁱ	3.30.572.10. 1 hit.
HAMAPⁱ	MF_00008. Thymidy_synth_bact. 1 hit.
InterProⁱ	View protein in InterPro IPR023451. Thymidate_synth/dCMP_Mease. IPR036926. Thymidate_synth/dCMP_Mease_sf. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00303. Thymidylat_synt. 1 hit.
PRINTSⁱ	PR00108. THYMDSNTHASE.
SUPFAMⁱ	SSF55831. SSF55831. 1 hit.
TIGRFAMsⁱ	TIGR03284. thym_sym. 2 hits.
PROSITEⁱ	View protein in PROSITE PS00091. THYMIDYLATE_SYNTHASE. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q8D2N4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKEYLDLLNL ILKNGYPKID RTKTGTLSMF GYQIRINLNE GFPLLTTKYC 
        60         70         80         90        100
HFKSIVYELL WFLRGDTNIS FLKKNNISIW NKWADKNGNL GPIYGKQWRA 
       110        120        130        140        150
WEDKKNNTID QIEIALNKLK KEPSSRRILV SSWNVGELDL MSIPPCHVLF 
       160        170        180        190        200
QLYVINNKLS CQVYQRSCDI FLGLPFNIGS YALLTHIFAN QCDLLVEDLI 
       210        220        230        240        250
WTGGDIHLYK NHLNQAKLQL TRSPLPLPKI FIKKKPKNLF NYAFNDFLLI 
       260 
DYNHHPKIKA PISI

Length:264

Mass (Da):30,804

Last modified:March 1, 2003 - v1

Checksum:ⁱ7D679A07D5EDBEA6

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BA000021 Genomic DNA. Translation: BAC24466.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011070124.1. NC_004344.2.

Genome annotation databases

EnsemblBacteriaⁱ	BAC24466; BAC24466; BAC24466.
KEGGⁱ	wbr:thyA.

Similar proteinsⁱ

50% Identity

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q8D2N4	P11044 A0A1B2AWY7 L8AKV1 G4EUQ8 A0A164XFS9 M4KU27 UPI0002C4F32B UPI000CDA178E UPI00084703DF UPI0002D864C1 +5251	Bacillus subtilis (strain 168) Bacillus subtilis subsp. subtilis Bacillus subtilis BEST7613 Bacillus subtilis subsp. subtilis str. SC-8 Bacillus subtilis Bacillus subtilis XF-1 Bacillus sp. 5B6 Bacillus halotolerans Bacillus Bacillus subtilis group And more	264	UniRef50_P11044	Cluster: Thymidylate synthase 2	5,262

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BA000021 Genomic DNA. Translation: BAC24466.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011070124.1. NC_004344.2.

3D structure databases

ProteinModelPortalⁱ	Q8D2N4.
SMRⁱ	Q8D2N4.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	36870.WGLp320.

STRINGⁱ

36870.WGLp320.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	BAC24466; BAC24466; BAC24466.
KEGGⁱ	wbr:thyA.

Phylogenomic databases

eggNOGⁱ	ENOG4105C0V. Bacteria. COG0207. LUCA.
KEGG Orthology (KO) More...KOⁱ	K00560.
OMAⁱ	KQYLDLC.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H02L6.

Enzyme and pathway databases

UniPathwayⁱ	UPA00575.

UniPathwayⁱ

UPA00575.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Dⁱ	3.30.572.10. 1 hit.
HAMAPⁱ	MF_00008. Thymidy_synth_bact. 1 hit.
InterProⁱ	View protein in InterPro IPR023451. Thymidate_synth/dCMP_Mease. IPR036926. Thymidate_synth/dCMP_Mease_sf. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00303. Thymidylat_synt. 1 hit.
PRINTSⁱ	PR00108. THYMDSNTHASE.
SUPFAMⁱ	SSF55831. SSF55831. 1 hit.
TIGRFAMsⁱ	TIGR03284. thym_sym. 2 hits.
PROSITEⁱ	View protein in PROSITE PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TYSY_WIGBR
Accessionⁱ	Q8D2N4Primary (citable) accession number: Q8D2N4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
	Last sequence update:	March 1, 2003
	Last modified:	October 25, 2017
	This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8D2N4 (TYSY_WIGBR)

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Thymidylate synthase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: dTTP biosynthesis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: dTTP biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ