UniProtKB - Q8D2N4 (TYSY_WIGBR)
Protein
Thymidylate synthase
Gene
thyA
Organism
Wigglesworthia glossinidia brevipalpis
Status
Functioni
Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation
Catalytic activityi
- EC:2.1.1.45UniRule annotation
: dTTP biosynthesis Pathwayi
This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationView all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 21 | dUMPUniRule annotation | 1 | |
Binding sitei | 51 | 5,10-methylenetetrahydrofolateUniRule annotation | 1 | |
Active sitei | 146 | NucleophileUniRule annotation | 1 | |
Binding sitei | 169 | 5,10-methylenetetrahydrofolateUniRule annotation | 1 | |
Binding sitei | 177 | dUMPUniRule annotation | 1 | |
Binding sitei | 263 | 5,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 126 – 127 | dUMP; shared with dimeric partnerUniRule annotation | 2 | |
Nucleotide bindingi | 166 – 169 | dUMPUniRule annotation | 4 | |
Nucleotide bindingi | 207 – 209 | dUMPUniRule annotation | 3 |
GO - Molecular functioni
- thymidylate synthase activity Source: UniProtKB-UniRule
GO - Biological processi
- dTMP biosynthetic process Source: UniProtKB-UniRule
- dTTP biosynthetic process Source: UniProtKB-UniPathway
- methylation Source: UniProtKB-KW
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Nucleotide biosynthesis |
Enzyme and pathway databases
UniPathwayi | UPA00575 |
Names & Taxonomyi
Protein namesi | Recommended name: Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)Short name: TSUniRule annotation Short name: TSaseUniRule annotation |
Gene namesi | Name:thyAUniRule annotation Ordered Locus Names:WIGBR3200 |
Organismi | Wigglesworthia glossinidia brevipalpis |
Taxonomic identifieri | 36870 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Erwiniaceae › Wigglesworthia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000141044 | 1 – 264 | Thymidylate synthaseAdd BLAST | 264 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 36870.25166276 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0207, Bacteria |
HOGENOMi | CLU_021669_0_0_6 |
OMAi | KQYLDLC |
OrthoDBi | 1212177at2 |
Family and domain databases
CDDi | cd00351, TS_Pyrimidine_HMase, 1 hit |
Gene3Di | 3.30.572.10, 1 hit |
HAMAPi | MF_00008, Thymidy_synth_bact, 1 hit |
InterProi | View protein in InterPro IPR023451, Thymidate_synth/dCMP_Mease IPR036926, Thymidate_synth/dCMP_Mease_sf IPR000398, Thymidylate_synthase IPR020940, Thymidylate_synthase_AS |
Pfami | View protein in Pfam PF00303, Thymidylat_synt, 1 hit |
PRINTSi | PR00108, THYMDSNTHASE |
SUPFAMi | SSF55831, SSF55831, 1 hit |
TIGRFAMsi | TIGR03284, thym_sym, 2 hits |
PROSITEi | View protein in PROSITE PS00091, THYMIDYLATE_SYNTHASE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8D2N4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKEYLDLLNL ILKNGYPKID RTKTGTLSMF GYQIRINLNE GFPLLTTKYC
60 70 80 90 100
HFKSIVYELL WFLRGDTNIS FLKKNNISIW NKWADKNGNL GPIYGKQWRA
110 120 130 140 150
WEDKKNNTID QIEIALNKLK KEPSSRRILV SSWNVGELDL MSIPPCHVLF
160 170 180 190 200
QLYVINNKLS CQVYQRSCDI FLGLPFNIGS YALLTHIFAN QCDLLVEDLI
210 220 230 240 250
WTGGDIHLYK NHLNQAKLQL TRSPLPLPKI FIKKKPKNLF NYAFNDFLLI
260
DYNHHPKIKA PISI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000021 Genomic DNA Translation: BAC24466.1 |
RefSeqi | WP_011070124.1, NC_004344.2 |
Genome annotation databases
EnsemblBacteriai | BAC24466; BAC24466; BAC24466 |
KEGGi | wbr:thyA |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000021 Genomic DNA Translation: BAC24466.1 |
RefSeqi | WP_011070124.1, NC_004344.2 |
3D structure databases
SMRi | Q8D2N4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 36870.25166276 |
Genome annotation databases
EnsemblBacteriai | BAC24466; BAC24466; BAC24466 |
KEGGi | wbr:thyA |
Phylogenomic databases
eggNOGi | COG0207, Bacteria |
HOGENOMi | CLU_021669_0_0_6 |
OMAi | KQYLDLC |
OrthoDBi | 1212177at2 |
Enzyme and pathway databases
UniPathwayi | UPA00575 |
Family and domain databases
CDDi | cd00351, TS_Pyrimidine_HMase, 1 hit |
Gene3Di | 3.30.572.10, 1 hit |
HAMAPi | MF_00008, Thymidy_synth_bact, 1 hit |
InterProi | View protein in InterPro IPR023451, Thymidate_synth/dCMP_Mease IPR036926, Thymidate_synth/dCMP_Mease_sf IPR000398, Thymidylate_synthase IPR020940, Thymidylate_synthase_AS |
Pfami | View protein in Pfam PF00303, Thymidylat_synt, 1 hit |
PRINTSi | PR00108, THYMDSNTHASE |
SUPFAMi | SSF55831, SSF55831, 1 hit |
TIGRFAMsi | TIGR03284, thym_sym, 2 hits |
PROSITEi | View protein in PROSITE PS00091, THYMIDYLATE_SYNTHASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TYSY_WIGBR | |
Accessioni | Q8D2N4Primary (citable) accession number: Q8D2N4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 23, 2003 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 92 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families