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Protein

Pyruvate formate-lyase-activating enzyme

Gene

pflA

Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi29Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi33Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi36Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Binding sitei79S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei207S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSEPI176280:G1G05-217-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase-activating enzyme (EC:1.97.1.4)
Short name:
PFL-activating enzyme
Gene namesi
Name:pflA
Ordered Locus Names:SE_0215
OrganismiStaphylococcus epidermidis (strain ATCC 12228)
Taxonomic identifieri176280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000001411 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002717181 – 251Pyruvate formate-lyase-activating enzymeAdd BLAST251

Interactioni

Protein-protein interaction databases

STRINGi176280.SE0215

Structurei

3D structure databases

ProteinModelPortaliQ8CTX5
SMRiQ8CTX5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 37S-adenosyl-L-methionine bindingBy similarity3
Regioni134 – 136S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105F1A Bacteria
COG1180 LUCA
KOiK04069
OMAiIGVPNKR

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR034457 Organic_radical-activating
IPR012839 Organic_radical_activase
IPR012838 PFL1_activating
IPR034465 Pyruvate_for-lyase_activase
IPR001989 Radical_activat_CS
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF000371 PFL_act_enz, 1 hit
SFLDiSFLDF00278 pyruvate_formate-lyase_activas, 1 hit
SFLDG01066 organic_radical-activating_enz, 1 hit
SFLDS00029 Radical_SAM, 1 hit
TIGRFAMsiTIGR02493 PFLA, 1 hit
PROSITEiView protein in PROSITE
PS01087 RADICAL_ACTIVATING, 1 hit

Sequencei

Sequence statusi: Complete.

Q8CTX5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKGHLHSVE SMGTVDGPGL RYILFTQGCL LRCLYCHNPD TWKINEPSRE
60 70 80 90 100
VTVDEMVNEI LPYKPYFEAS GGGVTVSGGE PLLQMPFLEQ LFKELKANGV
110 120 130 140 150
HTCIDTSAGC VNDTPAFNRH FDELQKHTDL ILLDIKHIDN DKHIKLTGKP
160 170 180 190 200
NTHILKFARK LSDMKQPVWI RHVLVPGISD DKEDLIKLGE FINSLDNVEK
210 220 230 240 250
FEILPYHQLG VHKWKNLGIP YQLENVEPPD DEAVKEAYRY VNFNGKIPVT

L
Length:251
Mass (Da):28,555
Last modified:March 1, 2003 - v1
Checksum:i90EC4BF0E80399E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015929 Genomic DNA Translation: AAO03812.1
RefSeqiNP_763770.1, NC_004461.1
WP_002446860.1, NC_004461.1

Genome annotation databases

EnsemblBacteriaiAAO03812; AAO03812; SE_0215
GeneIDi1056332
KEGGisep:SE0215
PATRICifig|176280.10.peg.194

Similar proteinsi

Entry informationi

Entry nameiPFLA_STAES
AccessioniPrimary (citable) accession number: Q8CTX5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: March 1, 2003
Last modified: February 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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