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Protein

Alanine dehydrogenase

Gene

ald

Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible reductive amination of pyruvate to L-alanine. May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer (By similarity).By similarity

Catalytic activityi

L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH.

Pathwayi: L-alanine degradation via dehydrogenase pathway

This protein is involved in step 1 of the subpathway that synthesizes NH(3) and pyruvate from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine dehydrogenase (ald)
This subpathway is part of the pathway L-alanine degradation via dehydrogenase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NH(3) and pyruvate from L-alanine, the pathway L-alanine degradation via dehydrogenase pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15SubstrateBy similarity1
Binding sitei74SubstrateBy similarity1
Active sitei95Proton donor/acceptorSequence analysis1
Binding sitei133NADBy similarity1
Binding sitei197NADBy similarity1
Binding sitei219NADBy similarity1
Active sitei269Proton donor/acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi177 – 178NADBy similarity2
Nucleotide bindingi238 – 239NADBy similarity2
Nucleotide bindingi266 – 269NADBy similarity4
Nucleotide bindingi298 – 301NADBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciSEPI176280:G1G05-1390-MONOMER
UniPathwayiUPA00527; UER00585

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine dehydrogenase (EC:1.4.1.1)
Gene namesi
Name:ald
Ordered Locus Names:SE_1384
OrganismiStaphylococcus epidermidis (strain ATCC 12228)
Taxonomic identifieri176280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000001411 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001990071 – 371Alanine dehydrogenaseAdd BLAST371

Interactioni

Subunit structurei

Homohexamer. Trimer of dimer (By similarity).By similarity

Protein-protein interaction databases

STRINGi176280.SE1384

Structurei

3D structure databases

ProteinModelPortaliQ8CNW8
SMRiQ8CNW8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AlaDH/PNT family.Curated

Phylogenomic databases

eggNOGiENOG4105DCF Bacteria
COG0686 LUCA
KOiK00259
OMAiCFETSKA

Family and domain databases

CDDicd05305 L-AlaDH, 1 hit
InterProiView protein in InterPro
IPR008141 Ala_DH
IPR008143 Ala_DH/PNT_CS2
IPR008142 AlaDH/PNT_CS1
IPR007886 AlaDH/PNT_N
IPR007698 AlaDH/PNT_NAD(H)-bd
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR42795 PTHR42795, 1 hit
PfamiView protein in Pfam
PF01262 AlaDh_PNT_C, 1 hit
PF05222 AlaDh_PNT_N, 1 hit
PIRSFiPIRSF000183 Alanine_dh, 1 hit
SMARTiView protein in SMART
SM01002 AlaDh_PNT_C, 1 hit
SM01003 AlaDh_PNT_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00518 alaDH, 1 hit
PROSITEiView protein in PROSITE
PS00836 ALADH_PNT_1, 1 hit
PS00837 ALADH_PNT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8CNW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGIPKEIK NNENRVGLSP SGVHALVDQG HEVLVETNAG LGSYFEDGDY
60 70 80 90 100
QEAGAKIVDE QSKAWDVDMV IKVKEPLESE YKFFKEELIL FTYLHLANEQ
110 120 130 140 150
KLTQALVDNK VISIAYETVQ LPDGSLPLLT PMSEVAGRMS TQVGAEFLQR
160 170 180 190 200
FNGGMGILLG GIPGVPKGKV TIIGGGQAGT NAAKIALGLG AEVTILDVNP
210 220 230 240 250
KRLEELEDLF DGRVRTIMSN PLNIEMYVKE SDLVIGAVLI PGAKAPNLVT
260 270 280 290 300
EDMIKEMKDG SVIVDIAIDQ GGIFETTDKI TTHDNPTYTK HGVVHYAVAN
310 320 330 340 350
MPGAVPRTST IGLNNATLPY AQLLANKGYR EAFKVNHPLS LGLNTFNGHV
360 370
TNKNVADTFN FEYTSIEDAL K
Length:371
Mass (Da):40,195
Last modified:March 1, 2003 - v1
Checksum:i78541923F8E7CD6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015929 Genomic DNA Translation: AAO04983.1
RefSeqiNP_764939.1, NC_004461.1
WP_001830775.1, NC_004461.1

Genome annotation databases

EnsemblBacteriaiAAO04983; AAO04983; SE_1384
GeneIDi1055853
KEGGisep:SE1384
PATRICifig|176280.10.peg.1352

Similar proteinsi

Entry informationi

Entry nameiDHA_STAES
AccessioniPrimary (citable) accession number: Q8CNW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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