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UniProtKB - Q8CJF7 (ELYS_MOUSE)

Protein

Protein ELYS

Gene

Ahctf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis (By similarity). Has also been proposed to function as a transcription factor which may play a specific role in hematopoietic tissues (PubMed:11952839).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi1955 – 1967A.T hookBy similarityAdd BLAST13

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA-binding transcription factor activity Source: MGI
View the complete GO annotation on QuickGO ...

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • hemopoiesis Source: UniProtKB
  • mRNA transport Source: UniProtKB-KW
  • nuclear pore complex assembly Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • regulation of cytokinesis Source: UniProtKB
  • regulation of transcription, DNA-templated Source: MGI
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processCell cycle, Cell division, Mitosis, mRNA transport, Protein transport, Transcription, Translocation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ELYS
Alternative name(s):
Embryonic large molecule derived from yolk sac
Protein MEL-28
Putative AT-hook-containing transcription factor 1
Gene namesi
Name:Ahctf1
Synonyms:Elys
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1915033 Ahctf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Kinetochore, Nuclear pore complex, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality before E7.5. Impaired proliferation of the inner cells of the blastocyst due at least in part to increased apoptosis.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi272 – 280FQEPENDPR → GSGSGSGSG: Abolishes nuclear pore localization; when associated with S-284. 1 Publication9
Mutagenesisi284Y → S: Abolishes nuclear pore localization; when associated with 272-GSGSGSGSG-280. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002463201 – 2243Protein ELYSAdd BLAST2243

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei528PhosphoserineCombined sources1
Modified residuei1080PhosphoserineCombined sources1
Modified residuei1083PhosphothreonineCombined sources1
Modified residuei1138PhosphoserineCombined sources1
Modified residuei1142PhosphoserineBy similarity1
Modified residuei1150PhosphoserineBy similarity1
Modified residuei1155PhosphoserineBy similarity1
Modified residuei1175PhosphothreonineBy similarity1
Modified residuei1214PhosphoserineCombined sources1
Modified residuei1218PhosphoserineCombined sources1
Modified residuei1222PhosphoserineBy similarity1
Modified residuei1235PhosphoserineBy similarity1
Modified residuei1381PhosphoserineCombined sources1
Modified residuei1517PhosphothreonineBy similarity1
Modified residuei1533PhosphoserineBy similarity1
Modified residuei1541PhosphoserineCombined sources1
Modified residuei1725PhosphoserineBy similarity1
Modified residuei1864PhosphoserineBy similarity1
Modified residuei1870PhosphoserineBy similarity1
Modified residuei1884PhosphoserineBy similarity1
Modified residuei1928PhosphoserineCombined sources1
Modified residuei1930PhosphoserineBy similarity1
Modified residuei1980PhosphoserineBy similarity1
Modified residuei2021PhosphoserineBy similarity1
Modified residuei2099PhosphoserineBy similarity1
Modified residuei2132PhosphoserineBy similarity1
Modified residuei2188PhosphoserineCombined sources1
Modified residuei2198PhosphoserineCombined sources1
Modified residuei2202PhosphoserineBy similarity1
Modified residuei2221N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8CJF7
MaxQBiQ8CJF7
PaxDbiQ8CJF7
PeptideAtlasiQ8CJF7
PRIDEiQ8CJF7

PTM databases

iPTMnetiQ8CJF7
PhosphoSitePlusiQ8CJF7

Expressioni

Tissue specificityi

Widely expressed with higher expression in testis, lung and kidney. Expressed in T-cells, B-cells and granulocytes in bone marrow.1 Publication

Developmental stagei

Expressed throughout the embryo at E3.5 and E6.5. Higher expression is detected at 10.5 dpc nad then progressively decreases. Highly expressed in fetal hematopoietic tissues including liver, spleen and thymus. Expressed in the endothelium lining the dorsal aorta of 11.5 dpc embryos (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000026491 Expressed in 291 organ(s), highest expression level in embryo
CleanExiMM_AHCTF1
ExpressionAtlasiQ8CJF7 baseline and differential
GenevisibleiQ8CJF7 MM

Interactioni

Subunit structurei

Associates with the Nup107-160 subcomplex of the NPC.By similarity

Protein-protein interaction databases

BioGridi230549, 4 interactors
IntActiQ8CJF7, 2 interactors
MINTiQ8CJF7
STRINGi10090.ENSMUSP00000027768

Structurei

Secondary structure

12243
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8CJF7
SMRiQ8CJF7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 981Necessary for cytoplasmic localization1 PublicationAdd BLAST981
Regioni1 – 494Seven-bladed beta propeller repeats1 PublicationAdd BLAST494
Regioni591 – 1092Important for nuclear localizationBy similarityAdd BLAST502
Regioni1019 – 2243Disordered1 PublicationAdd BLAST1225
Regioni1149 – 2243Necessary for nuclear localization1 PublicationAdd BLAST1095
Regioni1447 – 1694Mediates transcriptional activity1 PublicationAdd BLAST248
Regioni1828 – 2243Important for nuclear localization and chromatin bindingBy similarityAdd BLAST416

Domaini

The N-terminus forms a highly conserved seven-bladed beta propeller decorated with long loops and mediates anchorage to the Nup107-160 subcomplex of the nuclear pore, synergistically with the central alpha domain. The disordered C-terminus is responsible for the interactions with chromatin (PubMed:23499022).1 Publication

Sequence similaritiesi

Belongs to the ELYS family.Curated

Phylogenomic databases

eggNOGiENOG410IG0H Eukaryota
ENOG410XQV6 LUCA
GeneTreeiENSGT00390000018900
HOGENOMiHOG000293244
InParanoidiQ8CJF7
OMAiGSCRFID
OrthoDBiEOG091G006H
PhylomeDBiQ8CJF7
TreeFamiTF350425

Family and domain databases

InterProiView protein in InterPro
IPR032040 ELYS-bb
IPR025151 ELYS_dom
PfamiView protein in Pfam
PF13934 ELYS, 1 hit
PF16687 ELYS-bb, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

Q8CJF7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQDLTAQVTS DLLHFPEVTI EALGEDEITL ESVLRGKFAA GKNGLACLAC 
        60         70         80         90        100
GPQLEVVNSL TGERLSAYRF SGVNEQPPVV LAVKEFSWHK RTGLLIGLEE 
       110        120        130        140        150
ADGSVLCLYD LGISRVVKAV VLPGRVTAIE PIINHGGASA STQHLHPSLR 
       160        170        180        190        200
WLFGVAAVVT DVGQILLIDL CLDDLSCSQN EVEASDLEVI TGIPAEVPHI 
       210        220        230        240        250
RERVMREGRH LCFQLVSPLG VAISTLSYIN RTNQLAVGFS DGYLALWNMK 
       260        270        280        290        300
SMKREYYTQL EGGRVPVHAV AFQEPENDPR NCCYLWAVQS TQDSEGDVLS 
       310        320        330        340        350
LHLLQLAFGD RKCLASGQIL YEGLEYCEER YTLDLAGGTF PLRGQTSNTK 
       360        370        380        390        400
LLGCQSIERF PSHGDREESM REALSPDTSV SVFTWQVNIY GQGKPSVYLG 
       410        420        430        440        450
LFDINRWYHA QMPDSLRSGE SLHNCSYFAL WSLDSVVSRT SPHHILDILV 
       460        470        480        490        500
HERSLNRGVP PSYPPPEQFF NPSTFNFDAT CLLDSGVIHV TCAGFQKETL 
       510        520        530        540        550
TFLKKSGPTL NEVIPDSYNR CLVAGLLSPR LIDIQPSSLS QEEQLEAILS 
       560        570        580        590        600
AAIQTSSLGL LTGYIRTWII EEQPNSAANL RFVLEWTWNK VVLTKEEFDR 
       610        620        630        640        650
LCVPLFDGSC RFIDPQTIQS IQQCHLLLSN LSTVLSCFAM EAQGITERGL 
       660        670        680        690        700
VDLSNKHMVT QLLCQYAHMV LWFCHSGLLP EGLDDALQLS RLRYNYPVIQ 
       710        720        730        740        750
NYYTSRRQKS ERSPRGKWNH DCLMIDGLVS QLGDEVEKLW KRDEGGTGRY 
       760        770        780        790        800
PPASIHALLD IYLLDNITEA SKHAITIYLL LDIMYSFPNK TDTPIESFPT 
       810        820        830        840        850
AFAISWGQVK LVQGFWLLDH NDYENGLDLL FHPVTAKPAS WQHSKIIEAF 
       860        870        880        890        900
MSQGEHKQAL RYLQTMKPTV SSSNEVILHL TVLLFNRCMV EAWNLLRQNS 
       910        920        930        940        950
NRVNIEELLK HAYEVCQEMG LMEDLLKLPF TNTEQECLVK FLQSSTSVEN 
       960        970        980        990       1000
HEFLLVHHLQ RANYISALKL NQILKNNLMS DRDPRLRERS VTRNSILDQY 
      1010       1020       1030       1040       1050
GKILPRVQRK LAVERAKPYH LSTSSVFHEV SRPKPLSAFP KKAITGTVLT 
      1060       1070       1080       1090       1100
RSTFISNVLS KIGEVWASHE PRNGVSLFNS PKTEQPSPVV HSFPHPELPE 
      1110       1120       1130       1140       1150
AFVGTPISNT SQRISRLLDL VVHPVPQPSQ CLEFIQQSPT RSPLCLLSSS 
      1160       1170       1180       1190       1200
LPLSSQFKRP HQNTSRPSEL LLLETPLIVK KAKSLALSAT SSGFAEFTPP 
      1210       1220       1230       1240       1250
SILRSGFRTT PLASPSLSPG RSLTPPFRVK ETRISFMEEG MNTHWTDRAT 
      1260       1270       1280       1290       1300
DDRNTKAFVS TSFHKCGLPA ETEWMKTSDK NTYFPLDVPA KGPQKVVAES 
      1310       1320       1330       1340       1350
LATHSGRLEK LDVSKEDSTA STRSDQTSLE YHDAPSPEDL EGAVFVSPKP 
      1360       1370       1380       1390       1400
ASSSTELTTN STLQTERDND KDAFKSEGAP SPVKKQIGTG DAAVEAFSEL 
      1410       1420       1430       1440       1450
SRLDPVERAE ASFAVSSVCE GETSTSNSKT SVLDGIVPIE SRTSILTADH 
      1460       1470       1480       1490       1500
KESVANTVAD VESSGSTSSK CPVTSERSLG QKLTLNLKED EIEAHVPKEN 
      1510       1520       1530       1540       1550
VGLPEESPRI SAAPSDTHEI HLIGCENLEV QNSEEEAKNL SFDELYPLGA 
      1560       1570       1580       1590       1600
EKLEYNLSTI EQQFCDLPDD KDSAECDAAE VDGELFVAQS NFTLILEGEE 
      1610       1620       1630       1640       1650
GEAEASDSAA PNMLPKATKE KPVCHREPHN QERVTDLPSA VTADQESHKV 
      1660       1670       1680       1690       1700
ETLPYVPEPV KVAIAENLLD VIKDTRSKEA TPVAAGEAGD EDGAVIVSKA 
      1710       1720       1730       1740       1750
AHSSRLTNST PKTVKEPHAE TVNTSQNDDM VSSRTLTRRQ HALSLNVTSE 
      1760       1770       1780       1790       1800
QEPSAVATPK KRTRKIKETP ESSERTCSDL KVAPENQLTA QSPPAPRRGK 
      1810       1820       1830       1840       1850
KKDVSQGTLP SSGAVEPEPE PQGTPGRLRL RTQPPEPAAE ETPSRTKVRL 
      1860       1870       1880       1890       1900
SSVRKGTPRR LKKSVENGQS TEILDDLKGS EAASHDGTVT ELRNANLEDT 
      1910       1920       1930       1940       1950
QNMEYKQDEH SDQQLPLKRK RVREREVSVS SVTEEPKLDS SQLPLQTGLD 
      1960       1970       1980       1990       2000
VPATPRKRGR PRKVVPLEAD GGTTGKEQTS PQKKDVPVVR RSTRNTPARN 
      2010       2020       2030       2040       2050
VSTLEKSVLV PNKEAALVVT SKRRPTKKSA EESSKDPSAA VSDLAGGAAH 
      2060       2070       2080       2090       2100
TESADRRDGL LAAAALTPSA QGTRTRSRRT MLLTDISEPK TEPLFPPPSV 
      2110       2120       2130       2140       2150
KVPKKKSKAE NMEAAAQLKE LVSDLSSQFV VSPPALRTRQ KSISNTSKLL 
      2160       2170       2180       2190       2200
GELESDPKPL EIIEQKPKRS RTVKTRASRN TGKGSSWSPP PVEIKLVSPL 
      2210       2220       2230       2240 
ASPVDEIKTG KPRKTAEIAG KTLGRGRKKP SSFPKQILRR KML
Length:2,243
Mass (Da):247,646
Last modified:March 1, 2003 - v1
Checksum:iEEEA09B70F2B5691
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6S6E4F6S6E4_MOUSE
Protein ELYS
Ahctf1
116Annotation score:
F6QTR3F6QTR3_MOUSE
Protein ELYS
Ahctf1
104Annotation score:
F6SJR1F6SJR1_MOUSE
Protein ELYS
Ahctf1
176Annotation score:
F6XUM6F6XUM6_MOUSE
Protein ELYS
Ahctf1
35Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1207F → L in BAB78517 (PubMed:11952839).Curated1
Sequence conflicti1379A → T in BAB78517 (PubMed:11952839).Curated1
Sequence conflicti1414A → G in BAB78517 (PubMed:11952839).Curated1
Sequence conflicti1718H → R in BAB78517 (PubMed:11952839).Curated1
Sequence conflicti2084T → A in BAC37101 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059278 mRNA Translation: BAB78517.1
AB081498 Genomic DNA Translation: BAC22610.1
BC138343 mRNA Translation: AAI38344.1
BC138344 mRNA Translation: AAI38345.1
AK078022 mRNA Translation: BAC37101.2 Sequence problems.
CCDSiCCDS15564.1
RefSeqiNP_080651.2, NM_026375.2
UniGeneiMm.128165

Genome annotation databases

EnsembliENSMUST00000027768; ENSMUSP00000027768; ENSMUSG00000026491
GeneIDi226747
KEGGimmu:226747
UCSCiuc011wxn.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB059278 mRNA Translation: BAB78517.1
AB081498 Genomic DNA Translation: BAC22610.1
BC138343 mRNA Translation: AAI38344.1
BC138344 mRNA Translation: AAI38345.1
AK078022 mRNA Translation: BAC37101.2 Sequence problems.
CCDSiCCDS15564.1
RefSeqiNP_080651.2, NM_026375.2
UniGeneiMm.128165

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I0OX-ray1.90A1-494[»]
ProteinModelPortaliQ8CJF7
SMRiQ8CJF7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230549, 4 interactors
IntActiQ8CJF7, 2 interactors
MINTiQ8CJF7
STRINGi10090.ENSMUSP00000027768

PTM databases

iPTMnetiQ8CJF7
PhosphoSitePlusiQ8CJF7

Proteomic databases

EPDiQ8CJF7
MaxQBiQ8CJF7
PaxDbiQ8CJF7
PeptideAtlasiQ8CJF7
PRIDEiQ8CJF7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027768; ENSMUSP00000027768; ENSMUSG00000026491
GeneIDi226747
KEGGimmu:226747
UCSCiuc011wxn.1 mouse

Organism-specific databases

CTDi25909
MGIiMGI:1915033 Ahctf1

Phylogenomic databases

eggNOGiENOG410IG0H Eukaryota
ENOG410XQV6 LUCA
GeneTreeiENSGT00390000018900
HOGENOMiHOG000293244
InParanoidiQ8CJF7
OMAiGSCRFID
OrthoDBiEOG091G006H
PhylomeDBiQ8CJF7
TreeFamiTF350425

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase

Miscellaneous databases

PROiPR:Q8CJF7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026491 Expressed in 291 organ(s), highest expression level in embryo
CleanExiMM_AHCTF1
ExpressionAtlasiQ8CJF7 baseline and differential
GenevisibleiQ8CJF7 MM

Family and domain databases

InterProiView protein in InterPro
IPR032040 ELYS-bb
IPR025151 ELYS_dom
PfamiView protein in Pfam
PF13934 ELYS, 1 hit
PF16687 ELYS-bb, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiELYS_MOUSE
AccessioniPrimary (citable) accession number: Q8CJF7
Secondary accession number(s): B2RRC8, Q8BVJ5, Q8VD55
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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