UniProtKB - Q8CJF7 (ELYS_MOUSE)
Protein
Protein ELYS
Gene
Ahctf1
Organism
Mus musculus (Mouse)
Status
Functioni
Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis (By similarity). Has also been proposed to function as a transcription factor which may play a specific role in hematopoietic tissues (PubMed:11952839).By similarity1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 1955 – 1967 | A.T hookBy similarityAdd BLAST | 13 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- hemopoiesis Source: UniProtKB
- mRNA transport Source: UniProtKB-KW
- nuclear pore complex assembly Source: UniProtKB
- protein transport Source: UniProtKB-KW
- regulation of cytokinesis Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Biological process | Cell cycle, Cell division, Mitosis, mRNA transport, Protein transport, Transcription, Translocation, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-5663220, RHO GTPases Activate Formins R-MMU-68877, Mitotic Prometaphase R-MMU-9615933, Postmitotic nuclear pore complex (NPC) reformation R-MMU-9648025, EML4 and NUDC in mitotic spindle formation |
Names & Taxonomyi
Protein namesi | Recommended name: Protein ELYSAlternative name(s): Embryonic large molecule derived from yolk sac Protein MEL-28 Putative AT-hook-containing transcription factor 1 |
Gene namesi | Name:Ahctf1 Synonyms:Elys |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915033, Ahctf1 |
Subcellular locationi
Nucleus
- nucleoplasm 1 Publication
- nuclear pore complex 1 Publication
- Nucleus matrix 1 Publication
Other locations
- kinetochore By similarity
- Cytoplasm 1 Publication
Note: Localizes to the nuclear pore complex (NPC) throughout interphase. Localizes to the kinetochore from prophase, and this appears to require the Nup107-160 subcomplex of the NPC. Localizes to the periphery of chromatin from late anaphase (By similarity).By similarity
Nucleus
- nuclear matrix Source: MGI
- nuclear membrane Source: MGI
- nuclear pore outer ring Source: Ensembl
- nucleoplasm Source: UniProtKB-SubCell
- nucleus Source: UniProtKB
Other locations
- chromatin Source: Ensembl
- condensed chromosome kinetochore Source: UniProtKB-SubCell
- cytoplasm Source: MGI
Keywords - Cellular componenti
Centromere, Chromosome, Cytoplasm, Kinetochore, Nuclear pore complex, NucleusPathology & Biotechi
Disruption phenotypei
Embryonic lethality before 7.5 dpc. Impaired proliferation of the inner cells of the blastocyst due at least in part to increased apoptosis.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 272 – 280 | FQEPENDPR → GSGSGSGSG: Abolishes nuclear pore localization; when associated with S-284. 1 Publication | 9 | |
Mutagenesisi | 284 | Y → S: Abolishes nuclear pore localization; when associated with 272-GSGSGSGSG-280. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000246320 | 1 – 2243 | Protein ELYSAdd BLAST | 2243 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 528 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1080 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1083 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 1138 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1142 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1150 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1155 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1175 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1214 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1218 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1222 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1235 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1381 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1517 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1533 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1541 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1725 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1864 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1870 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1884 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1928 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1930 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1980 | PhosphoserineBy similarity | 1 | |
Modified residuei | 2021 | PhosphoserineBy similarity | 1 | |
Modified residuei | 2099 | PhosphoserineBy similarity | 1 | |
Modified residuei | 2132 | PhosphoserineBy similarity | 1 | |
Modified residuei | 2188 | PhosphoserineCombined sources | 1 | |
Modified residuei | 2198 | PhosphoserineCombined sources | 1 | |
Modified residuei | 2202 | PhosphoserineBy similarity | 1 | |
Modified residuei | 2221 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8CJF7 |
jPOSTi | Q8CJF7 |
MaxQBi | Q8CJF7 |
PaxDbi | Q8CJF7 |
PeptideAtlasi | Q8CJF7 |
PRIDEi | Q8CJF7 |
PTM databases
iPTMneti | Q8CJF7 |
PhosphoSitePlusi | Q8CJF7 |
Expressioni
Tissue specificityi
Widely expressed with higher expression in testis, lung and kidney. Expressed in T-cells, B-cells and granulocytes in bone marrow.1 Publication
Developmental stagei
Expressed throughout the embryo at 3.5 dpc and 6.5 dpc. Higher expression is detected at 10.5 dpc nad then progressively decreases. Highly expressed in fetal hematopoietic tissues including liver, spleen and thymus. Expressed in the endothelium lining the dorsal aorta of 11.5 dpc embryos (at protein level).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000026491, Expressed in forelimb bud and 306 other tissues |
Genevisiblei | Q8CJF7, MM |
Interactioni
Subunit structurei
Associates with the Nup107-160 subcomplex of the NPC.
By similarityProtein-protein interaction databases
BioGRIDi | 230549, 4 interactors |
ComplexPortali | CPX-4474, Nuclear pore complex |
IntActi | Q8CJF7, 2 interactors |
MINTi | Q8CJF7 |
STRINGi | 10090.ENSMUSP00000027768 |
Miscellaneous databases
RNActi | Q8CJF7, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8CJF7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 981 | Necessary for cytoplasmic localization1 PublicationAdd BLAST | 981 | |
Regioni | 1 – 494 | Seven-bladed beta propeller repeats1 PublicationAdd BLAST | 494 | |
Regioni | 591 – 1092 | Important for nuclear localizationBy similarityAdd BLAST | 502 | |
Regioni | 1019 – 2243 | Disordered1 PublicationAdd BLAST | 1225 | |
Regioni | 1149 – 2243 | Necessary for nuclear localization1 PublicationAdd BLAST | 1095 | |
Regioni | 1447 – 1694 | Mediates transcriptional activity1 PublicationAdd BLAST | 248 | |
Regioni | 1828 – 2243 | Important for nuclear localization and chromatin bindingBy similarityAdd BLAST | 416 |
Domaini
The N-terminus forms a highly conserved seven-bladed beta propeller decorated with long loops and mediates anchorage to the Nup107-160 subcomplex of the nuclear pore, synergistically with the central alpha domain. The disordered C-terminus is responsible for the interactions with chromatin (PubMed:23499022).1 Publication
Sequence similaritiesi
Belongs to the ELYS family.Curated
Phylogenomic databases
eggNOGi | ENOG502QU0D, Eukaryota |
GeneTreei | ENSGT00390000018900 |
HOGENOMi | CLU_001145_0_0_1 |
InParanoidi | Q8CJF7 |
OMAi | TSPHYIL |
OrthoDBi | 1040332at2759 |
PhylomeDBi | Q8CJF7 |
TreeFami | TF350425 |
Family and domain databases
DisProti | DP01548 |
InterProi | View protein in InterPro IPR032040, ELYS-bb IPR025151, ELYS_dom |
Pfami | View protein in Pfam PF13934, ELYS, 1 hit PF16687, ELYS-bb, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q8CJF7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQDLTAQVTS DLLHFPEVTI EALGEDEITL ESVLRGKFAA GKNGLACLAC
60 70 80 90 100
GPQLEVVNSL TGERLSAYRF SGVNEQPPVV LAVKEFSWHK RTGLLIGLEE
110 120 130 140 150
ADGSVLCLYD LGISRVVKAV VLPGRVTAIE PIINHGGASA STQHLHPSLR
160 170 180 190 200
WLFGVAAVVT DVGQILLIDL CLDDLSCSQN EVEASDLEVI TGIPAEVPHI
210 220 230 240 250
RERVMREGRH LCFQLVSPLG VAISTLSYIN RTNQLAVGFS DGYLALWNMK
260 270 280 290 300
SMKREYYTQL EGGRVPVHAV AFQEPENDPR NCCYLWAVQS TQDSEGDVLS
310 320 330 340 350
LHLLQLAFGD RKCLASGQIL YEGLEYCEER YTLDLAGGTF PLRGQTSNTK
360 370 380 390 400
LLGCQSIERF PSHGDREESM REALSPDTSV SVFTWQVNIY GQGKPSVYLG
410 420 430 440 450
LFDINRWYHA QMPDSLRSGE SLHNCSYFAL WSLDSVVSRT SPHHILDILV
460 470 480 490 500
HERSLNRGVP PSYPPPEQFF NPSTFNFDAT CLLDSGVIHV TCAGFQKETL
510 520 530 540 550
TFLKKSGPTL NEVIPDSYNR CLVAGLLSPR LIDIQPSSLS QEEQLEAILS
560 570 580 590 600
AAIQTSSLGL LTGYIRTWII EEQPNSAANL RFVLEWTWNK VVLTKEEFDR
610 620 630 640 650
LCVPLFDGSC RFIDPQTIQS IQQCHLLLSN LSTVLSCFAM EAQGITERGL
660 670 680 690 700
VDLSNKHMVT QLLCQYAHMV LWFCHSGLLP EGLDDALQLS RLRYNYPVIQ
710 720 730 740 750
NYYTSRRQKS ERSPRGKWNH DCLMIDGLVS QLGDEVEKLW KRDEGGTGRY
760 770 780 790 800
PPASIHALLD IYLLDNITEA SKHAITIYLL LDIMYSFPNK TDTPIESFPT
810 820 830 840 850
AFAISWGQVK LVQGFWLLDH NDYENGLDLL FHPVTAKPAS WQHSKIIEAF
860 870 880 890 900
MSQGEHKQAL RYLQTMKPTV SSSNEVILHL TVLLFNRCMV EAWNLLRQNS
910 920 930 940 950
NRVNIEELLK HAYEVCQEMG LMEDLLKLPF TNTEQECLVK FLQSSTSVEN
960 970 980 990 1000
HEFLLVHHLQ RANYISALKL NQILKNNLMS DRDPRLRERS VTRNSILDQY
1010 1020 1030 1040 1050
GKILPRVQRK LAVERAKPYH LSTSSVFHEV SRPKPLSAFP KKAITGTVLT
1060 1070 1080 1090 1100
RSTFISNVLS KIGEVWASHE PRNGVSLFNS PKTEQPSPVV HSFPHPELPE
1110 1120 1130 1140 1150
AFVGTPISNT SQRISRLLDL VVHPVPQPSQ CLEFIQQSPT RSPLCLLSSS
1160 1170 1180 1190 1200
LPLSSQFKRP HQNTSRPSEL LLLETPLIVK KAKSLALSAT SSGFAEFTPP
1210 1220 1230 1240 1250
SILRSGFRTT PLASPSLSPG RSLTPPFRVK ETRISFMEEG MNTHWTDRAT
1260 1270 1280 1290 1300
DDRNTKAFVS TSFHKCGLPA ETEWMKTSDK NTYFPLDVPA KGPQKVVAES
1310 1320 1330 1340 1350
LATHSGRLEK LDVSKEDSTA STRSDQTSLE YHDAPSPEDL EGAVFVSPKP
1360 1370 1380 1390 1400
ASSSTELTTN STLQTERDND KDAFKSEGAP SPVKKQIGTG DAAVEAFSEL
1410 1420 1430 1440 1450
SRLDPVERAE ASFAVSSVCE GETSTSNSKT SVLDGIVPIE SRTSILTADH
1460 1470 1480 1490 1500
KESVANTVAD VESSGSTSSK CPVTSERSLG QKLTLNLKED EIEAHVPKEN
1510 1520 1530 1540 1550
VGLPEESPRI SAAPSDTHEI HLIGCENLEV QNSEEEAKNL SFDELYPLGA
1560 1570 1580 1590 1600
EKLEYNLSTI EQQFCDLPDD KDSAECDAAE VDGELFVAQS NFTLILEGEE
1610 1620 1630 1640 1650
GEAEASDSAA PNMLPKATKE KPVCHREPHN QERVTDLPSA VTADQESHKV
1660 1670 1680 1690 1700
ETLPYVPEPV KVAIAENLLD VIKDTRSKEA TPVAAGEAGD EDGAVIVSKA
1710 1720 1730 1740 1750
AHSSRLTNST PKTVKEPHAE TVNTSQNDDM VSSRTLTRRQ HALSLNVTSE
1760 1770 1780 1790 1800
QEPSAVATPK KRTRKIKETP ESSERTCSDL KVAPENQLTA QSPPAPRRGK
1810 1820 1830 1840 1850
KKDVSQGTLP SSGAVEPEPE PQGTPGRLRL RTQPPEPAAE ETPSRTKVRL
1860 1870 1880 1890 1900
SSVRKGTPRR LKKSVENGQS TEILDDLKGS EAASHDGTVT ELRNANLEDT
1910 1920 1930 1940 1950
QNMEYKQDEH SDQQLPLKRK RVREREVSVS SVTEEPKLDS SQLPLQTGLD
1960 1970 1980 1990 2000
VPATPRKRGR PRKVVPLEAD GGTTGKEQTS PQKKDVPVVR RSTRNTPARN
2010 2020 2030 2040 2050
VSTLEKSVLV PNKEAALVVT SKRRPTKKSA EESSKDPSAA VSDLAGGAAH
2060 2070 2080 2090 2100
TESADRRDGL LAAAALTPSA QGTRTRSRRT MLLTDISEPK TEPLFPPPSV
2110 2120 2130 2140 2150
KVPKKKSKAE NMEAAAQLKE LVSDLSSQFV VSPPALRTRQ KSISNTSKLL
2160 2170 2180 2190 2200
GELESDPKPL EIIEQKPKRS RTVKTRASRN TGKGSSWSPP PVEIKLVSPL
2210 2220 2230 2240
ASPVDEIKTG KPRKTAEIAG KTLGRGRKKP SSFPKQILRR KML
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6QTR3 | F6QTR3_MOUSE | Protein ELYS | Ahctf1 | 104 | Annotation score: | ||
F6SJR1 | F6SJR1_MOUSE | Protein ELYS | Ahctf1 | 176 | Annotation score: | ||
F6S6E4 | F6S6E4_MOUSE | Protein ELYS | Ahctf1 | 116 | Annotation score: | ||
F6XUM6 | F6XUM6_MOUSE | Protein ELYS | Ahctf1 | 35 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1207 | F → L in BAB78517 (PubMed:11952839).Curated | 1 | |
Sequence conflicti | 1379 | A → T in BAB78517 (PubMed:11952839).Curated | 1 | |
Sequence conflicti | 1414 | A → G in BAB78517 (PubMed:11952839).Curated | 1 | |
Sequence conflicti | 1718 | H → R in BAB78517 (PubMed:11952839).Curated | 1 | |
Sequence conflicti | 2084 | T → A in BAC37101 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB059278 mRNA Translation: BAB78517.1 AB081498 Genomic DNA Translation: BAC22610.1 BC138343 mRNA Translation: AAI38344.1 BC138344 mRNA Translation: AAI38345.1 AK078022 mRNA Translation: BAC37101.2 Sequence problems. |
CCDSi | CCDS15564.1 |
RefSeqi | NP_080651.2, NM_026375.2 |
Genome annotation databases
Ensembli | ENSMUST00000027768; ENSMUSP00000027768; ENSMUSG00000026491 |
GeneIDi | 226747 |
KEGGi | mmu:226747 |
UCSCi | uc011wxn.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB059278 mRNA Translation: BAB78517.1 AB081498 Genomic DNA Translation: BAC22610.1 BC138343 mRNA Translation: AAI38344.1 BC138344 mRNA Translation: AAI38345.1 AK078022 mRNA Translation: BAC37101.2 Sequence problems. |
CCDSi | CCDS15564.1 |
RefSeqi | NP_080651.2, NM_026375.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4I0O | X-ray | 1.90 | A | 1-494 | [»] | |
SMRi | Q8CJF7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 230549, 4 interactors |
ComplexPortali | CPX-4474, Nuclear pore complex |
IntActi | Q8CJF7, 2 interactors |
MINTi | Q8CJF7 |
STRINGi | 10090.ENSMUSP00000027768 |
PTM databases
iPTMneti | Q8CJF7 |
PhosphoSitePlusi | Q8CJF7 |
Proteomic databases
EPDi | Q8CJF7 |
jPOSTi | Q8CJF7 |
MaxQBi | Q8CJF7 |
PaxDbi | Q8CJF7 |
PeptideAtlasi | Q8CJF7 |
PRIDEi | Q8CJF7 |
Protocols and materials databases
Antibodypediai | 20836, 110 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000027768; ENSMUSP00000027768; ENSMUSG00000026491 |
GeneIDi | 226747 |
KEGGi | mmu:226747 |
UCSCi | uc011wxn.1, mouse |
Organism-specific databases
CTDi | 25909 |
MGIi | MGI:1915033, Ahctf1 |
Phylogenomic databases
eggNOGi | ENOG502QU0D, Eukaryota |
GeneTreei | ENSGT00390000018900 |
HOGENOMi | CLU_001145_0_0_1 |
InParanoidi | Q8CJF7 |
OMAi | TSPHYIL |
OrthoDBi | 1040332at2759 |
PhylomeDBi | Q8CJF7 |
TreeFami | TF350425 |
Enzyme and pathway databases
Reactomei | R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-5663220, RHO GTPases Activate Formins R-MMU-68877, Mitotic Prometaphase R-MMU-9615933, Postmitotic nuclear pore complex (NPC) reformation R-MMU-9648025, EML4 and NUDC in mitotic spindle formation |
Miscellaneous databases
BioGRID-ORCSi | 226747, 7 hits in 18 CRISPR screens |
ChiTaRSi | Ahctf1, mouse |
PROi | PR:Q8CJF7 |
RNActi | Q8CJF7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026491, Expressed in forelimb bud and 306 other tissues |
Genevisiblei | Q8CJF7, MM |
Family and domain databases
DisProti | DP01548 |
InterProi | View protein in InterPro IPR032040, ELYS-bb IPR025151, ELYS_dom |
Pfami | View protein in Pfam PF13934, ELYS, 1 hit PF16687, ELYS-bb, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ELYS_MOUSE | |
Accessioni | Q8CJF7Primary (citable) accession number: Q8CJF7 Secondary accession number(s): B2RRC8, Q8BVJ5, Q8VD55 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 25, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families