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Protein

Cdc42-interacting protein 4

Gene

Trip10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL (By similarity). Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei166Mediates end-to-end attachment of dimersBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-194840 Rho GTPase cycle
R-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Cdc42-interacting protein 4
Alternative name(s):
Thyroid receptor-interacting protein 10
Short name:
TR-interacting protein 10
Short name:
TRIP-10
Gene namesi
Name:Trip10
Synonyms:Cip4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2146901 Trip10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi454I → S: Impairs interaction with CDC42 and RHOQ and reduces insulin-stimulated translocation to the plasma membrane. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002614391 – 603Cdc42-interacting protein 4Add BLAST603

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei296PhosphoserineCombined sources1
Modified residuei298PhosphoserineBy similarity1
Modified residuei299PhosphoserineBy similarity1
Modified residuei335PhosphoserineBy similarity1
Modified residuei351PhosphoserineBy similarity1
Modified residuei482PhosphoserineBy similarity1

Post-translational modificationi

Tyrosine phosphorylated. Also phosphorylated by PKA (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PeptideAtlasiQ8CJ53
PRIDEiQ8CJ53

PTM databases

iPTMnetiQ8CJ53
PhosphoSitePlusiQ8CJ53

Expressioni

Gene expression databases

BgeeiENSMUSG00000019487 Expressed in 221 organ(s), highest expression level in heart left ventricle
CleanExiMM_TRIP10
ExpressionAtlasiQ8CJ53 baseline and differential
GenevisibleiQ8CJ53 MM

Interactioni

Subunit structurei

Homodimerizes, the dimers can polymerize end-to-end to form filamentous structures (By similarity). Interacts with AKAP9, ARHGAP17, DAAM1, DIAPH1, DIAPH2, DNM1, FASLG/FASL, GAPVD1, LYN, microtubules, PDE6G, SRC and WAS/WASP. Interacts with the ligand binding domain of the thyroid receptor (TR) in the presence of thyroid hormone. May interact with CTNNB1 and HD/HTT (By similarity). Interacts specifically with GTP-bound CDC42 and RHOQ. Interacts with DNM2 and WASL.By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi223098, 1 interactor

Structurei

3D structure databases

ProteinModelPortaliQ8CJ53
SMRiQ8CJ53
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 264F-BARPROSITE-ProRule annotationAdd BLAST264
Domaini393 – 470REM-1PROSITE-ProRule annotationAdd BLAST78
Domaini542 – 603SH3PROSITE-ProRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 117Required for podosome formation and interaction with AKAP9 and microtubulesBy similarityAdd BLAST117
Regioni1 – 117Required for translocation to the plasma membrane in response to insulinAdd BLAST117
Regioni293 – 603Interaction with PDE6GBy similarityAdd BLAST311
Regioni293 – 539Interaction with CDC42By similarityAdd BLAST247
Regioni471 – 603Required for interaction with FASLG and localization to lysosomesBy similarityAdd BLAST133
Regioni487 – 543Interaction with DNM2 and WASL1 PublicationAdd BLAST57
Regioni532 – 603Interaction with DNM1 and WASLBy similarityAdd BLAST72
Regioni540 – 603Required for podosome formationBy similarityAdd BLAST64
Regioni546 – 603Interaction with WASBy similarityAdd BLAST58
Regioni548 – 603Interaction with ARHGAP17, DAAM1, DIAPH1 and DIAPH2By similarityAdd BLAST56

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili67 – 259By similarityAdd BLAST193
Coiled coili388 – 481By similarityAdd BLAST94

Domaini

The F-BAR domain binds the phospholipid membrane with its concave surface. The end-to-end polymerization of dimers of these domains provides a curved surface that fits best membranes with around 600 A diameter, and may drive tubulation (By similarity).By similarity

Sequence similaritiesi

Belongs to the FNBP1 family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

GeneTreeiENSGT00510000046403
HOGENOMiHOG000231767
HOVERGENiHBG002489
InParanoidiQ8CJ53
KOiK07196
OMAiPTVTEDF
OrthoDBiEOG091G038P
PhylomeDBiQ8CJ53
TreeFamiTF351162

Family and domain databases

Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR028498 CIP4
IPR031160 F_BAR
IPR001060 FCH_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR43990:SF2 PTHR43990:SF2, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF00018 SH3_1, 1 hit
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS51860 REM_1, 1 hit
PS50002 SH3, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8CJ53-1) [UniParc]FASTAAdd to basket
Also known as: Cip4/2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDWGTELWDQ FEVLERHTQW GLDLLDKYVK FVKERAEVEQ AYAKQLRSLV
60 70 80 90 100
KKYLPKRPTK DDPEVKFSQQ QSFVQLLQEV NDFAGQRELV AESLGIRVCL
110 120 130 140 150
ELAKYSQEMK QERKMHFQEG RRAQQQLENG FKQLENSKRK FERDCREAEK
160 170 180 190 200
AAHTAERLDQ DINATKADVE KAKQQAHLRN HMAEESKNEY AAQLQRFNRD
210 220 230 240 250
QAHFYFSQMP QIFDKLQDMD ERRATRLGAG YGLLSEAELQ VVPIIGKCLE
260 270 280 290 300
GMKVAAESVD AKNDSQVLIE LHKSGFARPG DLEFEDFSQV INRVPSDSSL
310 320 330 340 350
GTPDGRPELR AASSRSRAKR WPFGKKNKPR PPSLSLLGGH LPSTLSDGPS
360 370 380 390 400
SPRSGRDPLA ILSEISKSVK PRLASFRSFR GGRGTVATED FSHLPPEQQR
410 420 430 440 450
KRLQQQLEER NRELQKEEDQ REALKKMKDV YEKTPQMGDP ASLEPRIAET
460 470 480 490 500
LGNIERLKLE VQKYEAWLAE AESRVLSNRG DSLSRHARPP DPPTTAPPDS
510 520 530 540 550
SSSSTNSGSQ DNKESSSEEP PSEGQDTPIY TEFDEDFEEP ASPIGQCVAI
560 570 580 590 600
YHFEGSSEGT VSMSEGEDLS LMEEDKGDGW TRVRRKQGAE GYVPTSYLRV

TLN
Length:603
Mass (Da):68,489
Last modified:November 28, 2006 - v2
Checksum:iDDA6271D5FD515AC
GO
Isoform 2 (identifier: Q8CJ53-2) [UniParc]FASTAAdd to basket
Also known as: H

The sequence of this isoform differs from the canonical sequence as follows:
     515-515: Missing.

Show »
Length:602
Mass (Da):68,402
Checksum:i165D63A9EC6CD884
GO
Isoform 3 (identifier: Q8CJ53-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     329-384: Missing.

Show »
Length:547
Mass (Da):62,654
Checksum:i5DE65DB3D6C9B8E2
GO
Isoform 4 (identifier: Q8CJ53-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     329-384: Missing.
     515-515: Missing.

Show »
Length:546
Mass (Da):62,567
Checksum:i8627CD20F0C19667
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A286YEA1A0A286YEA1_MOUSE
Cdc42-interacting protein 4
Trip10
41Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti321W → C in AAN38709 (PubMed:12242347).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021722329 – 384Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST56
Alternative sequenceiVSP_021723515Missing in isoform 2 and isoform 4. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY081142 mRNA Translation: AAL89589.1
AF502565 mRNA Translation: AAN38709.1
AK088909 mRNA Translation: BAC40648.1
AK149902 mRNA Translation: BAE29155.1
BC003249 mRNA Translation: AAH03249.1
CCDSiCCDS28930.1 [Q8CJ53-3]
RefSeqiNP_001229318.1, NM_001242389.1 [Q8CJ53-1]
NP_001229319.1, NM_001242390.1 [Q8CJ53-2]
NP_001229320.1, NM_001242391.1 [Q8CJ53-4]
NP_598886.1, NM_134125.4 [Q8CJ53-3]
UniGeneiMm.37368

Genome annotation databases

EnsembliENSMUST00000019631; ENSMUSP00000019631; ENSMUSG00000019487 [Q8CJ53-4]
ENSMUST00000224152; ENSMUSP00000153081; ENSMUSG00000019487 [Q8CJ53-1]
ENSMUST00000224885; ENSMUSP00000153576; ENSMUSG00000019487 [Q8CJ53-2]
ENSMUST00000224947; ENSMUSP00000153467; ENSMUSG00000019487 [Q8CJ53-3]
GeneIDi106628
KEGGimmu:106628
UCSCiuc008dej.2 mouse [Q8CJ53-3]
uc008dek.2 mouse [Q8CJ53-1]
uc008del.2 mouse [Q8CJ53-2]
uc008dem.2 mouse [Q8CJ53-4]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY081142 mRNA Translation: AAL89589.1
AF502565 mRNA Translation: AAN38709.1
AK088909 mRNA Translation: BAC40648.1
AK149902 mRNA Translation: BAE29155.1
BC003249 mRNA Translation: AAH03249.1
CCDSiCCDS28930.1 [Q8CJ53-3]
RefSeqiNP_001229318.1, NM_001242389.1 [Q8CJ53-1]
NP_001229319.1, NM_001242390.1 [Q8CJ53-2]
NP_001229320.1, NM_001242391.1 [Q8CJ53-4]
NP_598886.1, NM_134125.4 [Q8CJ53-3]
UniGeneiMm.37368

3D structure databases

ProteinModelPortaliQ8CJ53
SMRiQ8CJ53
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi223098, 1 interactor

PTM databases

iPTMnetiQ8CJ53
PhosphoSitePlusiQ8CJ53

Proteomic databases

PeptideAtlasiQ8CJ53
PRIDEiQ8CJ53

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019631; ENSMUSP00000019631; ENSMUSG00000019487 [Q8CJ53-4]
ENSMUST00000224152; ENSMUSP00000153081; ENSMUSG00000019487 [Q8CJ53-1]
ENSMUST00000224885; ENSMUSP00000153576; ENSMUSG00000019487 [Q8CJ53-2]
ENSMUST00000224947; ENSMUSP00000153467; ENSMUSG00000019487 [Q8CJ53-3]
GeneIDi106628
KEGGimmu:106628
UCSCiuc008dej.2 mouse [Q8CJ53-3]
uc008dek.2 mouse [Q8CJ53-1]
uc008del.2 mouse [Q8CJ53-2]
uc008dem.2 mouse [Q8CJ53-4]

Organism-specific databases

CTDi9322
MGIiMGI:2146901 Trip10

Phylogenomic databases

GeneTreeiENSGT00510000046403
HOGENOMiHOG000231767
HOVERGENiHBG002489
InParanoidiQ8CJ53
KOiK07196
OMAiPTVTEDF
OrthoDBiEOG091G038P
PhylomeDBiQ8CJ53
TreeFamiTF351162

Enzyme and pathway databases

ReactomeiR-MMU-194840 Rho GTPase cycle
R-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

PROiPR:Q8CJ53
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019487 Expressed in 221 organ(s), highest expression level in heart left ventricle
CleanExiMM_TRIP10
ExpressionAtlasiQ8CJ53 baseline and differential
GenevisibleiQ8CJ53 MM

Family and domain databases

Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR028498 CIP4
IPR031160 F_BAR
IPR001060 FCH_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR43990:SF2 PTHR43990:SF2, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF00018 SH3_1, 1 hit
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS51860 REM_1, 1 hit
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCIP4_MOUSE
AccessioniPrimary (citable) accession number: Q8CJ53
Secondary accession number(s): Q8BTR8, Q8R433, Q99LI0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: November 7, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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