UniProtKB - Q8CIV8 (TBCE_MOUSE)
Tubulin-specific chaperone E
Tbce
Functioni
Tubulin-folding protein; involved in the second step of the tubulin folding pathway and in the regulation of tubulin heterodimer dissociation (PubMed:12389029, PubMed:17184771).
Required for correct organization of microtubule cytoskeleton and mitotic splindle, and maintenance of the neuronal microtubule network (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- alpha-tubulin binding Source: GO_Central
- unfolded protein binding Source: MGI
GO - Biological processi
- adult locomotory behavior Source: MGI
- axonogenesis Source: MGI
- developmental growth Source: MGI
- microtubule cytoskeleton organization Source: MGI
- mitotic spindle organization Source: UniProtKB
- muscle atrophy Source: MGI
- peripheral nervous system neuron axonogenesis Source: MGI
- post-chaperonin tubulin folding pathway Source: UniProtKB
- post-embryonic development Source: MGI
- protein folding Source: MGI
- tubulin complex assembly Source: GO_Central
Keywordsi
Molecular function | Chaperone |
Names & Taxonomyi
Protein namesi | Recommended name: Tubulin-specific chaperone EAlternative name(s): Tubulin-folding cofactor E |
Gene namesi | Name:Tbce |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1917680, Tbce |
VEuPathDBi | HostDB:ENSMUSG00000039233 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytoskeleton
- cytoskeleton By similarity
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000083539 | 2 – 524 | Tubulin-specific chaperone EAdd BLAST | 523 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 460 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 492 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8CIV8 |
MaxQBi | Q8CIV8 |
PaxDbi | Q8CIV8 |
PeptideAtlasi | Q8CIV8 |
PRIDEi | Q8CIV8 |
ProteomicsDBi | 263136 |
PTM databases
PhosphoSitePlusi | Q8CIV8 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000039233, Expressed in undifferentiated genital tubercle and 313 other tissues |
Genevisiblei | Q8CIV8, MM |
Interactioni
Subunit structurei
Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state. Cofactors B and E can form a heterodimer which binds to alpha-tubulin and enhances their ability to dissociate tubulin heterodimers.
Interacts with TBCD (By similarity).
By similarity1 PublicationGO - Molecular functioni
- alpha-tubulin binding Source: GO_Central
- unfolded protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 214045, 6 interactors |
STRINGi | 10090.ENSMUSP00000047880 |
Miscellaneous databases
RNActi | Q8CIV8, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q8CIV8 |
SMRi | Q8CIV8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8CIV8 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 71 | CAP-GlyPROSITE-ProRule annotationAdd BLAST | 45 | |
Repeati | 154 – 175 | LRR 1Add BLAST | 22 | |
Repeati | 180 – 201 | LRR 2Add BLAST | 22 | |
Repeati | 206 – 227 | LRR 3Add BLAST | 22 | |
Repeati | 231 – 253 | LRR 4Add BLAST | 23 | |
Repeati | 254 – 275 | LRR 5Add BLAST | 22 | |
Repeati | 279 – 300 | LRR 6Add BLAST | 22 | |
Repeati | 309 – 330 | LRR 7Add BLAST | 22 | |
Domaini | 343 – 381 | LRRCTAdd BLAST | 39 |
Sequence similaritiesi
Keywords - Domaini
Leucine-rich repeat, RepeatPhylogenomic databases
eggNOGi | KOG3207, Eukaryota |
GeneTreei | ENSGT00530000063405 |
HOGENOMi | CLU_017716_5_0_1 |
InParanoidi | Q8CIV8 |
OMAi | NAEMFYL |
OrthoDBi | 249920at2759 |
PhylomeDBi | Q8CIV8 |
TreeFami | TF313455 |
Family and domain databases
CDDi | cd17044, Ubl_TBCE, 1 hit |
Gene3Di | 2.30.30.190, 1 hit 3.80.10.10, 2 hits |
InterProi | View protein in InterPro IPR036859, CAP-Gly_dom_sf IPR000938, CAP-Gly_domain IPR032675, LRR_dom_sf IPR044994, TBCE IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR044079, Ubl_TBCE |
PANTHERi | PTHR15140, PTHR15140, 1 hit |
Pfami | View protein in Pfam PF01302, CAP_GLY, 1 hit PF14560, Ubiquitin_2, 1 hit |
SMARTi | View protein in SMART SM01052, CAP_GLY, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF74924, SSF74924, 1 hit |
PROSITEi | View protein in PROSITE PS00845, CAP_GLY_1, 1 hit PS50245, CAP_GLY_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSDILPLDVI GRRVEVNGEY ATVRFCGAVP PVAGLWLGVE WDNPERGKHD
60 70 80 90 100
GSHEGTMYFK CRHPTGGSFV RPSKVNFGDD FLTALKKRYV LEDGPDDDEN
110 120 130 140 150
SCSLKVGSKQ VQTIGFEHIT KKQSQLRALQ DISLWNCAVS HAGEQGRIAE
160 170 180 190 200
ACPNIRVVNL SKNLLSTWDE VVLIAEQLKD LEALDLSENK LQFPSDSPTL
210 220 230 240 250
TRTFSTLKTL VLNKTGITWT EVLHCAPSWP VLEELYLKSN NISISERPVN
260 270 280 290 300
VLQKMRLLDL SSNPSIDESQ LSLIADLPRL EHLVLSDIGL SSIHFPDAEI
310 320 330 340 350
GCKTSMFPAL KYLIVNDNQI SEWSFINELD KLQSLQALSC TRNPLSKADK
360 370 380 390 400
AEEIIIAKIA QLRTLNRCQI LPEERRGAEL DYRKAFGNEW RKAGGHPDPD
410 420 430 440 450
KNRPNAAFLS AHPRYQLLCC KYGAPEDEEL KTQQPFMLKK QLLTLKIKCS
460 470 480 490 500
NQPERQILEK QLPDSMTVQK VKGLLSRLLK VPVSELLLSY ESSKMPGREI
510 520
ELENDLQPLQ FYSVENGDCL LVRW
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE0CZ81 | E0CZ81_MOUSE | Tubulin-specific chaperone E | Tbce | 428 | Annotation score: | ||
E0CXJ6 | E0CXJ6_MOUSE | Tubulin-specific chaperone E | Tbce | 75 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 348 | A → V1 Publication | 1 | |
Natural varianti | 524 | W → G1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY082332 mRNA Translation: AAL92570.1 AK167383 mRNA Translation: BAE39475.1 BC050206 mRNA Translation: AAH50206.1 |
CCDSi | CCDS26247.1 |
RefSeqi | NP_848027.1, NM_178337.3 |
Genome annotation databases
Ensembli | ENSMUST00000039894; ENSMUSP00000047880; ENSMUSG00000039233 |
GeneIDi | 70430 |
KEGGi | mmu:70430 |
UCSCi | uc007pmq.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY082332 mRNA Translation: AAL92570.1 AK167383 mRNA Translation: BAE39475.1 BC050206 mRNA Translation: AAH50206.1 |
CCDSi | CCDS26247.1 |
RefSeqi | NP_848027.1, NM_178337.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WJN | NMR | - | A | 441-524 | [»] | |
BMRBi | Q8CIV8 | |||||
SMRi | Q8CIV8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 214045, 6 interactors |
STRINGi | 10090.ENSMUSP00000047880 |
PTM databases
PhosphoSitePlusi | Q8CIV8 |
Proteomic databases
EPDi | Q8CIV8 |
MaxQBi | Q8CIV8 |
PaxDbi | Q8CIV8 |
PeptideAtlasi | Q8CIV8 |
PRIDEi | Q8CIV8 |
ProteomicsDBi | 263136 |
Protocols and materials databases
DNASUi | 70430 |
Genome annotation databases
Ensembli | ENSMUST00000039894; ENSMUSP00000047880; ENSMUSG00000039233 |
GeneIDi | 70430 |
KEGGi | mmu:70430 |
UCSCi | uc007pmq.2, mouse |
Organism-specific databases
CTDi | 6905 |
MGIi | MGI:1917680, Tbce |
VEuPathDBi | HostDB:ENSMUSG00000039233 |
Phylogenomic databases
eggNOGi | KOG3207, Eukaryota |
GeneTreei | ENSGT00530000063405 |
HOGENOMi | CLU_017716_5_0_1 |
InParanoidi | Q8CIV8 |
OMAi | NAEMFYL |
OrthoDBi | 249920at2759 |
PhylomeDBi | Q8CIV8 |
TreeFami | TF313455 |
Miscellaneous databases
BioGRID-ORCSi | 70430, 20 hits in 50 CRISPR screens |
ChiTaRSi | Tbce, mouse |
EvolutionaryTracei | Q8CIV8 |
PROi | PR:Q8CIV8 |
RNActi | Q8CIV8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039233, Expressed in undifferentiated genital tubercle and 313 other tissues |
Genevisiblei | Q8CIV8, MM |
Family and domain databases
CDDi | cd17044, Ubl_TBCE, 1 hit |
Gene3Di | 2.30.30.190, 1 hit 3.80.10.10, 2 hits |
InterProi | View protein in InterPro IPR036859, CAP-Gly_dom_sf IPR000938, CAP-Gly_domain IPR032675, LRR_dom_sf IPR044994, TBCE IPR000626, Ubiquitin-like_dom IPR029071, Ubiquitin-like_domsf IPR044079, Ubl_TBCE |
PANTHERi | PTHR15140, PTHR15140, 1 hit |
Pfami | View protein in Pfam PF01302, CAP_GLY, 1 hit PF14560, Ubiquitin_2, 1 hit |
SMARTi | View protein in SMART SM01052, CAP_GLY, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF74924, SSF74924, 1 hit |
PROSITEi | View protein in PROSITE PS00845, CAP_GLY_1, 1 hit PS50245, CAP_GLY_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TBCE_MOUSE | |
Accessioni | Q8CIV8Primary (citable) accession number: Q8CIV8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 7, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families