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UniProtKB - Q8CIM3 (D2HDH_MOUSE)
Protein
D-2-hydroxyglutarate dehydrogenase, mitochondrial
Gene
D2hgdh
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the oxidation of D-2-hydroxyglutarate (D-2-HG) to alpha-ketoglutarate (By similarity).
Also catalyzes the oxidation of other D-2-hydroxyacids, such as D-malate (D-MAL) and D-lactate (D-LAC) (By similarity).
Exhibits high activities towards D-2-HG and D-MAL but a very weak activity towards D-LAC (By similarity).
By similarityCatalytic activityi
Cofactori
FADCurated
Activity regulationi
Activated by zinc and cobalt ions.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 400 | D-2-hydroxyglutarateBy similarity | 1 | |
Binding sitei | 400 | D-lactateBy similarity | 1 | |
Binding sitei | 400 | D-malateBy similarity | 1 | |
Binding sitei | 404 | D-2-hydroxyglutarateBy similarity | 1 | |
Binding sitei | 404 | D-malateBy similarity | 1 | |
Binding sitei | 415 | D-2-hydroxyglutarateBy similarity | 1 | |
Binding sitei | 415 | D-malateBy similarity | 1 | |
Metal bindingi | 448 | ZincBy similarity | 1 | |
Metal bindingi | 455 | ZincBy similarity | 1 | |
Binding sitei | 457 | D-2-hydroxyglutarateBy similarity | 1 | |
Metal bindingi | 489 | ZincBy similarity | 1 | |
Binding sitei | 490 | D-2-hydroxyglutarateBy similarity | 1 | |
Binding sitei | 490 | D-lactateBy similarity | 1 | |
Binding sitei | 490 | D-malateBy similarity | 1 |
GO - Molecular functioni
- (R)-2-hydroxyglutarate dehydrogenase activity Source: UniProtKB
- FAD binding Source: InterPro
- zinc ion binding Source: UniProtKB
GO - Biological processi
- cellular protein metabolic process Source: HGNC-UCL
- malate metabolic process Source: UniProtKB
- response to cobalt ion Source: HGNC-UCL
- response to manganese ion Source: HGNC-UCL
- response to zinc ion Source: HGNC-UCL
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-880009, Interconversion of 2-oxoglutarate and 2-hydroxyglutarate |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:D2hgdh |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2138209, D2hgdh |
VEuPathDBi | HostDB:ENSMUSG00000073609 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Mitochondrion
- mitochondrion Source: HGNC-UCL
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 50 | MitochondrionSequence analysisAdd BLAST | 50 | |
ChainiPRO_0000231675 | 51 – 535 | D-2-hydroxyglutarate dehydrogenase, mitochondrialAdd BLAST | 485 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 115 | N6-succinyllysineCombined sources | 1 |
Proteomic databases
MaxQBi | Q8CIM3 |
PaxDbi | Q8CIM3 |
PeptideAtlasi | Q8CIM3 |
PRIDEi | Q8CIM3 |
ProteomicsDBi | 279872 |
PTM databases
iPTMneti | Q8CIM3 |
PhosphoSitePlusi | Q8CIM3 |
SwissPalmi | Q8CIM3 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000073609, Expressed in mesenteric lymph node and 266 other tissues |
Genevisiblei | Q8CIM3, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 221035, 1 interactor |
STRINGi | 10090.ENSMUSP00000095235 |
Miscellaneous databases
RNActi | Q8CIM3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 110 – 289 | FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST | 180 |
Sequence similaritiesi
Belongs to the FAD-binding oxidoreductase/transferase type 4 family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1232, Eukaryota |
GeneTreei | ENSGT00550000075086 |
HOGENOMi | CLU_017779_4_1_1 |
InParanoidi | Q8CIM3 |
OMAi | TPRTCGE |
OrthoDBi | 515900at2759 |
TreeFami | TF323342 |
Family and domain databases
Gene3Di | 1.10.45.10, 1 hit 3.30.43.10, 1 hit 3.30.465.10, 1 hit |
InterProi | View protein in InterPro IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR016164, FAD-linked_Oxase-like_C IPR004113, FAD-linked_oxidase_C IPR006094, Oxid_FAD_bind_N IPR016171, Vanillyl_alc_oxidase_C-sub2 |
Pfami | View protein in Pfam PF02913, FAD-oxidase_C, 1 hit PF01565, FAD_binding_4, 1 hit |
SUPFAMi | SSF55103, SSF55103, 1 hit SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Q8CIM3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVLPLVSRWS ARVLWASPGW RRTYTQRACV GLKRLGCPRG VYSPLAHRAY
60 70 80 90 100
SVVAGGPEVT LTPERYPVQR LPFSTVSEED LAAFECIIPG RVITDPEQLQ
110 120 130 140 150
TCNVDWLKTV RGCSKVLLRP QTSEEVSQIL RHCYKRNLAV NPQGGNTGMV
160 170 180 190 200
GGSVPVFDEV ILSTALMNQV ISFHDVSGIL VCQAGCVLEE LSRYVQERDF
210 220 230 240 250
IMPLDLGAKG SCHIGGNVAT NAGGLRFLRY GSLRGTVLGL EVVLADGTIL
260 270 280 290 300
NCLTSLRKDN TGYDLKQMFI GSEGTLGVIT AVSIVCPPRP KAVNVAFLGC
310 320 330 340 350
PGFAEVLQTF RTCRGMLGEI LSAFEFMDTE CMQLVGQHLQ LTNPVQESPF
360 370 380 390 400
YVLVETSGSS AGHDAEKLTN VLEQVLNSGL VTDGTMATDQ RKVQMLWALR
410 420 430 440 450
ERITEALSRD GYVFKYDLSL PVERLYDLVI DLRTRLGPRA KHVVGYGHLG
460 470 480 490 500
DGNLHLNVTA EAFSRELLGA LEPYVYAWTA EQRGSVSAEH GLGFKKKDVL
510 520 530
GYSKPPVAVT LMQQLKAMLD PEGILNPYKT LPAQA
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9QN44 | E9QN44_MOUSE | D-2-hydroxyglutarate dehydrogenase,... | D2hgdh | 557 | Annotation score: | ||
A0A087WR78 | A0A087WR78_MOUSE | D-2-hydroxyglutarate dehydrogenase,... | D2hgdh | 219 | Annotation score: | ||
A0A087WPG6 | A0A087WPG6_MOUSE | D-2-hydroxyglutarate dehydrogenase,... | D2hgdh | 37 | Annotation score: | ||
A0A087WQG0 | A0A087WQG0_MOUSE | D-2-hydroxyglutarate dehydrogenase,... | D2hgdh | 50 | Annotation score: | ||
A0A087WPK4 | A0A087WPK4_MOUSE | D-2-hydroxyglutarate dehydrogenase,... | D2hgdh | 77 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 56 | G → S in AAH23277 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 451 | D → G in BAE41647 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 522 | E → K in BAE41647 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 522 | E → K in BAC40205 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 522 | E → K in AAH23277 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 522 | E → K in AAI17795 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK088200 mRNA Translation: BAC40205.1 AK170226 mRNA Translation: BAE41647.1 AC167139 Genomic DNA No translation available. BC023277 mRNA Translation: AAH23277.1 BC117794 mRNA Translation: AAI17795.1 |
CCDSi | CCDS15196.1 |
RefSeqi | NP_849213.2, NM_178882.4 |
Genome annotation databases
Ensembli | ENSMUST00000097633; ENSMUSP00000095235; ENSMUSG00000073609 |
GeneIDi | 98314 |
KEGGi | mmu:98314 |
UCSCi | uc007cep.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK088200 mRNA Translation: BAC40205.1 AK170226 mRNA Translation: BAE41647.1 AC167139 Genomic DNA No translation available. BC023277 mRNA Translation: AAH23277.1 BC117794 mRNA Translation: AAI17795.1 |
CCDSi | CCDS15196.1 |
RefSeqi | NP_849213.2, NM_178882.4 |
3D structure databases
SMRi | Q8CIM3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 221035, 1 interactor |
STRINGi | 10090.ENSMUSP00000095235 |
PTM databases
iPTMneti | Q8CIM3 |
PhosphoSitePlusi | Q8CIM3 |
SwissPalmi | Q8CIM3 |
Proteomic databases
MaxQBi | Q8CIM3 |
PaxDbi | Q8CIM3 |
PeptideAtlasi | Q8CIM3 |
PRIDEi | Q8CIM3 |
ProteomicsDBi | 279872 |
Protocols and materials databases
Antibodypediai | 34576, 113 antibodies from 18 providers |
Genome annotation databases
Ensembli | ENSMUST00000097633; ENSMUSP00000095235; ENSMUSG00000073609 |
GeneIDi | 98314 |
KEGGi | mmu:98314 |
UCSCi | uc007cep.2, mouse |
Organism-specific databases
CTDi | 728294 |
MGIi | MGI:2138209, D2hgdh |
VEuPathDBi | HostDB:ENSMUSG00000073609 |
Phylogenomic databases
eggNOGi | KOG1232, Eukaryota |
GeneTreei | ENSGT00550000075086 |
HOGENOMi | CLU_017779_4_1_1 |
InParanoidi | Q8CIM3 |
OMAi | TPRTCGE |
OrthoDBi | 515900at2759 |
TreeFami | TF323342 |
Enzyme and pathway databases
Reactomei | R-MMU-880009, Interconversion of 2-oxoglutarate and 2-hydroxyglutarate |
Miscellaneous databases
BioGRID-ORCSi | 98314, 2 hits in 63 CRISPR screens |
ChiTaRSi | D2hgdh, mouse |
PROi | PR:Q8CIM3 |
RNActi | Q8CIM3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000073609, Expressed in mesenteric lymph node and 266 other tissues |
Genevisiblei | Q8CIM3, MM |
Family and domain databases
Gene3Di | 1.10.45.10, 1 hit 3.30.43.10, 1 hit 3.30.465.10, 1 hit |
InterProi | View protein in InterPro IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR016164, FAD-linked_Oxase-like_C IPR004113, FAD-linked_oxidase_C IPR006094, Oxid_FAD_bind_N IPR016171, Vanillyl_alc_oxidase_C-sub2 |
Pfami | View protein in Pfam PF02913, FAD-oxidase_C, 1 hit PF01565, FAD_binding_4, 1 hit |
SUPFAMi | SSF55103, SSF55103, 1 hit SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | D2HDH_MOUSE | |
Accessioni | Q8CIM3Primary (citable) accession number: Q8CIM3 Secondary accession number(s): E9QLL1 Q8BU06 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 4, 2006 |
Last sequence update: | October 3, 2012 | |
Last modified: | February 23, 2022 | |
This is version 130 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families