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Protein

DIS3-like exonuclease 2

Gene

Dis3l2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of both mRNAs and miRNAs that have been polyuridylated by a terminal uridylyltransferase, such as ZCCHC11/TUT4. Mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'. Mediates degradation of uridylated pre-let-7 miRNAs, contributing to the maintenance of embryonic stem (ES) cells. Essential for correct mitosis, and negatively regulates cell proliferation.UniRule annotation2 Publications

Cofactori

Mg2+UniRule annotation1 Publication, Mn2+UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi381Magnesium1 Publication1
Sitei389Important for catalytic activity1 Publication1
Metal bindingi390Magnesium1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease, RNA-binding
Biological processCell cycle, Cell division, Mitosis
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DIS3-like exonuclease 2UniRule annotation (EC:3.1.13.-UniRule annotation)
Gene namesi
Name:Dis3l2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2442555 Dis3l2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi74R → A: Loss of enzyme activity and polyuridylated RNA-binding; when associated with A-612. 1 Publication1
Mutagenesisi389D → N: Loss of exoribonuclease activity. 1 Publication1
Mutagenesisi548 – 553RLDQLK → AAAAAA: 40% decreased enzyme activity. 1 Publication6
Mutagenesisi612Q → A: Loss of enzyme activity and polyuridylated RNA-binding; when associated with A-74. 1 Publication1
Mutagenesisi777N → A: 40% increased enzyme activity. 1 Publication1
Mutagenesisi778Q → A: Impaired enzyme activity and polyuridylated RNA-binding. 1 Publication1
Mutagenesisi796N → A: Does not affect enzyme activity. 1 Publication1

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003148181 – 870DIS3-like exonuclease 2Add BLAST870

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31PhosphoserineBy similarity1
Modified residuei172PhosphoserineBy similarity1
Modified residuei250N6-acetyllysineBy similarity1
Modified residuei864PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8CI75
PaxDbiQ8CI75
PeptideAtlasiQ8CI75
PRIDEiQ8CI75

PTM databases

iPTMnetiQ8CI75
PhosphoSitePlusiQ8CI75

Expressioni

Gene expression databases

BgeeiENSMUSG00000053333 Expressed in 209 organ(s), highest expression level in brain
ExpressionAtlasiQ8CI75 baseline and differential
GenevisibleiQ8CI75 MM

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Lin28aQ8K3Y32EBI-16045218,EBI-11109197

Protein-protein interaction databases

DIPiDIP-60224N
IntActiQ8CI75, 1 interactor
STRINGi10090.ENSMUSP00000132673

Structurei

Secondary structure

1870
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8CI75
SMRiQ8CI75
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Specifically recognizes and binds polyuridylated RNAs via 3 RNA-binding regions (named U-zone 1, U-zone 2 and U-zone 3) that form an open funnel on one face of the catalytic domain, allowing RNA to navigate a path to the active site.1 Publication

Sequence similaritiesi

Belongs to the RNR ribonuclease family. DIS3L2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2102 Eukaryota
COG0557 LUCA
GeneTreeiENSGT00530000063106
HOGENOMiHOG000017705
HOVERGENiHBG107810
InParanoidiQ8CI75
KOiK18758
OMAiREFEAFC
OrthoDBiEOG091G06EJ
PhylomeDBiQ8CI75
TreeFamiTF315191

Family and domain databases

HAMAPiMF_03045 DIS3L2, 1 hit
InterProiView protein in InterPro
IPR028591 DIS3L2
IPR012340 NA-bd_OB-fold
IPR001900 RNase_II/R
IPR022966 RNase_II/R_CS
IPR033771 Rrp44_CSD1
PfamiView protein in Pfam
PF00773 RNB, 1 hit
PF17216 Rrp44_CSD1, 1 hit
SMARTiView protein in SMART
SM00955 RNB, 1 hit
SUPFAMiSSF50249 SSF50249, 2 hits
PROSITEiView protein in PROSITE
PS01175 RIBONUCLEASE_II, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8CI75-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNHPDYKLNL RSPGTPRGVS SVVGPSAVGA SPGDKKSKNK SMRGKKKSIF
60 70 80 90 100
ETYMSKEDVS EGLKRGTLIQ GVLRINPKKF HEAFIPSPDG DRDIFIDGVV
110 120 130 140 150
ARNRALNGDL VVVKLLPEDQ WKAVKPESND KEIEATYEAD IPEEGCGHHP
160 170 180 190 200
LQQSRKGWSG PDVIIEAQFD DSDSEDRHGN TSGLVDGVKK LSISTPDRGK
210 220 230 240 250
EDSSTPVMKD ENTPIPQDTR GLSEKSLQKS AKVVYILEKK HSRAATGILK
260 270 280 290 300
LLADKNSDLF KKYALFSPSD HRVPRIYVPL KDCPQDFMTR PKDFANTLFI
310 320 330 340 350
CRIIDWKEDC NFALGQLAKS LGQAGEIEPE TEGILTEYGV DFSDFSSEVL
360 370 380 390 400
ECLPQSLPWT IPPDEVGKRR DLRKDCIFTI DPSTARDLDD ALACRRLTDG
410 420 430 440 450
TFEVGVHIAD VSYFVPEGSS LDKVAAERAT SVYLVQKVVP MLPRLLCEEL
460 470 480 490 500
CSLNPMTDKL TFSVIWKLTP EGKILEEWFG RTIIRSCTKL SYDHAQSMIE
510 520 530 540 550
NPTEKIPEEE LPPISPEHSV EEVHQAVLNL HSIAKQLRRQ RFVDGALRLD
560 570 580 590 600
QLKLAFTLDH ETGLPQGCHI YEYRDSNKLV EEFMLLANMA VAHKIFRTFP
610 620 630 640 650
EQALLRRHPP PQTKMLSDLV EFCDQMGLPM DVSSAGALNK SLTKTFGDDK
660 670 680 690 700
YSLARKEVLT NMYSRPMQMA LYFCSGMLQD QEQFRHYALN VPLYTHFTSP
710 720 730 740 750
IRRFADVIVH RLLAAALGYS EQPDVEPDTL QKQADHCNDR RMASKRVQEL
760 770 780 790 800
SIGLFFAVLV KESGPLESEA MVMGVLNQAF DVLVLRFGVQ KRIYCNALAL
810 820 830 840 850
RSYSFQKVGK KPELTLVWEP DDLEEEPTQQ VITIFSLVDV VLQAEATALK
860 870
YSAILKRPGL EKASDEEPED
Length:870
Mass (Da):97,775
Last modified:March 1, 2003 - v1
Checksum:iE54474450DCD363F
GO
Isoform 2 (identifier: Q8CI75-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     122-122: K → KPRITLSLPGVLGLQ

Note: No experimental confirmation available.
Show »
Length:884
Mass (Da):99,220
Checksum:iFDD0AEB9C4F05712
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WP08A0A087WP08_MOUSE
DIS3-like exonuclease 2
Dis3l2
123Annotation score:

Sequence cautioni

The sequence BAC27292 differs from that shown. Reason: Frameshift at position 53.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_030379122K → KPRITLSLPGVLGLQ in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031180 mRNA Translation: BAC27292.1 Frameshift.
BC036177 mRNA Translation: AAH36177.1
CCDSiCCDS15124.1 [Q8CI75-1]
CCDS48304.1 [Q8CI75-2]
RefSeqiNP_001165628.1, NM_001172157.1 [Q8CI75-2]
NP_705758.1, NM_153530.2 [Q8CI75-1]
UniGeneiMm.389152

Genome annotation databases

EnsembliENSMUST00000065694; ENSMUSP00000070506; ENSMUSG00000053333 [Q8CI75-1]
ENSMUST00000168237; ENSMUSP00000132673; ENSMUSG00000053333 [Q8CI75-2]
GeneIDi208718
KEGGimmu:208718
UCSCiuc007bvu.2 mouse [Q8CI75-1]
uc007bvw.2 mouse [Q8CI75-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK031180 mRNA Translation: BAC27292.1 Frameshift.
BC036177 mRNA Translation: AAH36177.1
CCDSiCCDS15124.1 [Q8CI75-1]
CCDS48304.1 [Q8CI75-2]
RefSeqiNP_001165628.1, NM_001172157.1 [Q8CI75-2]
NP_705758.1, NM_153530.2 [Q8CI75-1]
UniGeneiMm.389152

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4PMWX-ray2.95A/B37-856[»]
ProteinModelPortaliQ8CI75
SMRiQ8CI75
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60224N
IntActiQ8CI75, 1 interactor
STRINGi10090.ENSMUSP00000132673

PTM databases

iPTMnetiQ8CI75
PhosphoSitePlusiQ8CI75

Proteomic databases

EPDiQ8CI75
PaxDbiQ8CI75
PeptideAtlasiQ8CI75
PRIDEiQ8CI75

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065694; ENSMUSP00000070506; ENSMUSG00000053333 [Q8CI75-1]
ENSMUST00000168237; ENSMUSP00000132673; ENSMUSG00000053333 [Q8CI75-2]
GeneIDi208718
KEGGimmu:208718
UCSCiuc007bvu.2 mouse [Q8CI75-1]
uc007bvw.2 mouse [Q8CI75-2]

Organism-specific databases

CTDi129563
MGIiMGI:2442555 Dis3l2

Phylogenomic databases

eggNOGiKOG2102 Eukaryota
COG0557 LUCA
GeneTreeiENSGT00530000063106
HOGENOMiHOG000017705
HOVERGENiHBG107810
InParanoidiQ8CI75
KOiK18758
OMAiREFEAFC
OrthoDBiEOG091G06EJ
PhylomeDBiQ8CI75
TreeFamiTF315191

Miscellaneous databases

PROiPR:Q8CI75
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000053333 Expressed in 209 organ(s), highest expression level in brain
ExpressionAtlasiQ8CI75 baseline and differential
GenevisibleiQ8CI75 MM

Family and domain databases

HAMAPiMF_03045 DIS3L2, 1 hit
InterProiView protein in InterPro
IPR028591 DIS3L2
IPR012340 NA-bd_OB-fold
IPR001900 RNase_II/R
IPR022966 RNase_II/R_CS
IPR033771 Rrp44_CSD1
PfamiView protein in Pfam
PF00773 RNB, 1 hit
PF17216 Rrp44_CSD1, 1 hit
SMARTiView protein in SMART
SM00955 RNB, 1 hit
SUPFAMiSSF50249 SSF50249, 2 hits
PROSITEiView protein in PROSITE
PS01175 RIBONUCLEASE_II, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDI3L2_MOUSE
AccessioniPrimary (citable) accession number: Q8CI75
Secondary accession number(s): Q8BMG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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