UniProtKB - Q8CI71 (VPS50_MOUSE)
Protein
Syndetin
Gene
Vps50
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane. Within the EARP complex, required to tether the complex to recycling endosomes. Not involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).By similarity
GO - Molecular functioni
- SNARE binding Source: MGI
GO - Biological processi
- endocytic recycling Source: MGI
- protein transport Source: UniProtKB-KW
Keywordsi
Biological process | Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: SyndetinBy similarityAlternative name(s): Bcl2-like protein blm1 Publication Coiled-coil domain-containing protein 132Curated EARP/GARPII complex subunit VPS50By similarity |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1920538, Vps50 |
Subcellular locationi
Endosome
- Recycling endosome By similarity
Other locations
- Membrane By similarity
Note: Associates with membranes in an EIPR1-dependent manner.By similarity
Cytosol
- cytosol Source: GOC
Endosome
- EARP complex Source: UniProtKB
- recycling endosome Source: UniProtKB
Other locations
- membrane Source: UniProtKB
- perinuclear region of cytoplasm Source: MGI
Keywords - Cellular componenti
Endosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000307266 | 1 – 964 | SyndetinAdd BLAST | 964 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 15 | PhosphoserineBy similarity | 1 | |
Modified residuei | 494 | PhosphoserineBy similarity | 1 | |
Modified residuei | 498 | PhosphoserineBy similarity | 1 | |
Modified residuei | 559 | PhosphoserineCombined sources | 1 | |
Modified residuei | 561 | PhosphoserineCombined sources | 1 | |
Cross-linki | 963 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 963 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8CI71 |
jPOSTi | Q8CI71 |
PaxDbi | Q8CI71 |
PeptideAtlasi | Q8CI71 |
PRIDEi | Q8CI71 |
PTM databases
iPTMneti | Q8CI71 |
PhosphoSitePlusi | Q8CI71 |
SwissPalmi | Q8CI71 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000001376, Expressed in visual cortex and 279 other tissues |
Genevisiblei | Q8CI71, MM |
Interactioni
Subunit structurei
Component of the endosome-associated retrograde protein (EARP) complex, composed of VPS51, VPS52, VPS53 and VPS50/Syndetin (By similarity). The EARP complex interacts with EIPR1 (By similarity).
Interacts with VPS51 and VPS53 in an EIPR1-independent manner (By similarity).
By similarityGO - Molecular functioni
- SNARE binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 215896, 5 interactors |
STRINGi | 10090.ENSMUSP00000001412 |
Miscellaneous databases
RNActi | Q8CI71, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 81 – 107 | Sequence analysisAdd BLAST | 27 | |
Coiled coili | 216 – 244 | Sequence analysisAdd BLAST | 29 |
Sequence similaritiesi
Belongs to the syndetin family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG2939, Eukaryota |
GeneTreei | ENSGT00390000003442 |
HOGENOMi | CLU_009513_1_0_1 |
InParanoidi | Q8CI71 |
OMAi | AKVKWDV |
OrthoDBi | 293145at2759 |
PhylomeDBi | Q8CI71 |
TreeFami | TF106152 |
Family and domain databases
InterProi | View protein in InterPro IPR019514, Syndetin_C IPR040047, VPS50 IPR019515, VPS54_N |
PANTHERi | PTHR13258, PTHR13258, 1 hit |
Pfami | View protein in Pfam PF10474, DUF2451, 1 hit PF10475, Vps54_N, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q8CI71-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MQKIKSLMTR QGLKSPPESL NDLGAFESLR VPGKEEFREL REQPSDPQAE
60 70 80 90 100
QELINSIEQV YFSADPFDIV KYELEKLPPV LNLQELEEYR DKLKQQQSAV
110 120 130 140 150
SKKVADLILE KQPAYVKELE RVTSLQTGLQ LAAVICTNGR RHLNIAKEGF
160 170 180 190 200
TQASLGLLAN QRKRQLLIGL LKSLRTIKTL QRTDIRLSEM LEEEDYPGAI
210 220 230 240 250
QLCLECQKAA STFKHYSCIS ELNSKLQDTL EQIEEQLDVA LSKICKNFDI
260 270 280 290 300
NHYTKVQQAY RLLGKTQTAM DQLHMHFTQA IHNTVFQVVL GYVELCAGNT
310 320 330 340 350
DTKFQKLQYK DLCTHVTPDS YIPCLADLCK ALWEVMLSYY RTMEWHEKHD
360 370 380 390 400
NEETAAAAEG SNVMSTEEAT FDRGYVKKKL EHGLTRIWQD VQLKVKTYLL
410 420 430 440 450
GTDLSIFKYD DFIFVLDIVS RLMQVGEEFC GSKSEVLQES IRKQSVNYFK
460 470 480 490 500
NHHRIRLDEL RMFLENETWE LCPVKSNFSI LQLHEFKFLE QSRSPSVSPS
510 520 530 540 550
KQPSATSSKP VTLFEQYCSG GNPFEIQADH KDEETEDVLA SNGYESDEQE
560 570 580 590 600
KSAYQDYDSD SDVPEELKRD YVDEQTGDVP VKSVSRETLK SRKKSDYSLN
610 620 630 640 650
KVNAPILTNT TLNVIRLVGK YMQMMNILKP IAFDVIHFMS QLFDYYLYAI
660 670 680 690 700
YTFFGRNDSL ESTGLGLSSS RLKTTLNRIQ ESLIDLEGSA DPTATLTAAE
710 720 730 740 750
ERKEKVPSPH LNQLVILTSG DTLYGLAERV VATESLVFLA EQFEFLQPHL
760 770 780 790 800
DAVMPAVKKP FLQQFYSQTV STASELRKPI YWIVAGKAID YEQMLLLMMN
810 820 830 840 850
VKWDVKEIMS QHNIYVDALL KEFEQFNKRL NEVSKRVRIP LPVSNILWEH
860 870 880 890 900
CIRLANRTIV EGYANVKKCS NEGRALMQLD FQQFLMKLEK LTDIRPIPDK
910 920 930 940 950
EFVETYIKAY YLTENDMERW IKEHREYSTK QLTNLVNVCL GSHINKKARQ
960
KLLAAIDEID RPKR
Sequence cautioni
The sequence AAH02304 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC98268 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 77 – 78 | LP → PS in BAC31137 (PubMed:16141072).Curated | 2 | |
Sequence conflicti | 89 – 96 | YRDKLKQQ → SRYQFKQH in BAC31137 (PubMed:16141072).Curated | 8 | |
Sequence conflicti | 160 | N → Y in BAC31137 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 429 | F → S in BAC36038 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 511 | V → M in AAH02304 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_028660 | 926 – 938 | EYSTK…NLVNV → VRTRVGVLILSGV in isoform 3. 1 PublicationAdd BLAST | 13 | |
Alternative sequenceiVSP_028661 | 926 – 929 | EYST → IFSV in isoform 2. 1 Publication | 4 | |
Alternative sequenceiVSP_028662 | 930 – 964 | Missing in isoform 2. 1 PublicationAdd BLAST | 35 | |
Alternative sequenceiVSP_028663 | 939 – 964 | Missing in isoform 3. 1 PublicationAdd BLAST | 26 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY254697 mRNA Translation: AAO84056.1 AK129458 mRNA Translation: BAC98268.1 Different initiation. AK042031 mRNA Translation: BAC31137.1 AK075898 mRNA Translation: BAC36038.1 BC002304 mRNA Translation: AAH02304.1 Different initiation. BC036294 mRNA Translation: AAH36294.2 BC043092 mRNA Translation: AAH43092.1 |
CCDSi | CCDS39415.1 [Q8CI71-1] CCDS51713.1 [Q8CI71-2] CCDS51714.1 [Q8CI71-3] |
RefSeqi | NP_001161222.1, NM_001167750.1 [Q8CI71-2] NP_001161223.1, NM_001167751.1 [Q8CI71-3] NP_077222.4, NM_024260.5 [Q8CI71-1] |
Genome annotation databases
Ensembli | ENSMUST00000001412; ENSMUSP00000001412; ENSMUSG00000001376 [Q8CI71-1] ENSMUST00000164052; ENSMUSP00000125872; ENSMUSG00000001376 [Q8CI71-2] ENSMUST00000170873; ENSMUSP00000128323; ENSMUSG00000001376 [Q8CI71-3] |
GeneIDi | 73288 |
KEGGi | mmu:73288 |
UCSCi | uc009avb.2, mouse [Q8CI71-3] uc009avc.2, mouse [Q8CI71-1] uc012ehr.1, mouse [Q8CI71-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY254697 mRNA Translation: AAO84056.1 AK129458 mRNA Translation: BAC98268.1 Different initiation. AK042031 mRNA Translation: BAC31137.1 AK075898 mRNA Translation: BAC36038.1 BC002304 mRNA Translation: AAH02304.1 Different initiation. BC036294 mRNA Translation: AAH36294.2 BC043092 mRNA Translation: AAH43092.1 |
CCDSi | CCDS39415.1 [Q8CI71-1] CCDS51713.1 [Q8CI71-2] CCDS51714.1 [Q8CI71-3] |
RefSeqi | NP_001161222.1, NM_001167750.1 [Q8CI71-2] NP_001161223.1, NM_001167751.1 [Q8CI71-3] NP_077222.4, NM_024260.5 [Q8CI71-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 215896, 5 interactors |
STRINGi | 10090.ENSMUSP00000001412 |
PTM databases
iPTMneti | Q8CI71 |
PhosphoSitePlusi | Q8CI71 |
SwissPalmi | Q8CI71 |
Proteomic databases
EPDi | Q8CI71 |
jPOSTi | Q8CI71 |
PaxDbi | Q8CI71 |
PeptideAtlasi | Q8CI71 |
PRIDEi | Q8CI71 |
Protocols and materials databases
Antibodypediai | 15650, 166 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000001412; ENSMUSP00000001412; ENSMUSG00000001376 [Q8CI71-1] ENSMUST00000164052; ENSMUSP00000125872; ENSMUSG00000001376 [Q8CI71-2] ENSMUST00000170873; ENSMUSP00000128323; ENSMUSG00000001376 [Q8CI71-3] |
GeneIDi | 73288 |
KEGGi | mmu:73288 |
UCSCi | uc009avb.2, mouse [Q8CI71-3] uc009avc.2, mouse [Q8CI71-1] uc012ehr.1, mouse [Q8CI71-2] |
Organism-specific databases
CTDi | 55610 |
MGIi | MGI:1920538, Vps50 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG2939, Eukaryota |
GeneTreei | ENSGT00390000003442 |
HOGENOMi | CLU_009513_1_0_1 |
InParanoidi | Q8CI71 |
OMAi | AKVKWDV |
OrthoDBi | 293145at2759 |
PhylomeDBi | Q8CI71 |
TreeFami | TF106152 |
Miscellaneous databases
BioGRID-ORCSi | 73288, 4 hits in 17 CRISPR screens |
ChiTaRSi | Vps50, mouse |
PROi | PR:Q8CI71 |
RNActi | Q8CI71, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000001376, Expressed in visual cortex and 279 other tissues |
Genevisiblei | Q8CI71, MM |
Family and domain databases
InterProi | View protein in InterPro IPR019514, Syndetin_C IPR040047, VPS50 IPR019515, VPS54_N |
PANTHERi | PTHR13258, PTHR13258, 1 hit |
Pfami | View protein in Pfam PF10474, DUF2451, 1 hit PF10475, Vps54_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VPS50_MOUSE | |
Accessioni | Q8CI71Primary (citable) accession number: Q8CI71 Secondary accession number(s): Q6ZPG9 Q99LN1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 23, 2007 |
Last sequence update: | May 24, 2005 | |
Last modified: | December 2, 2020 | |
This is version 113 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families