UniProtKB - Q8CI59 (STEA3_MOUSE)
Metalloreductase STEAP3
Steap3
Functioni
Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe3+ to Fe2+. Also mediates reduction of Cu2+ to Cu1+, suggesting that it participates in copper homeostasis. Uses NADP+ as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.
2 PublicationsCofactori
Protein has several cofactor binding sites:Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 116 | NADP; via amide nitrogenBy similarity | 1 | |
Binding sitei | 151 | NADP; via amide nitrogenBy similarity | 1 | |
Binding sitei | 152 | FADBy similarity | 1 | |
Binding sitei | 160 | FADBy similarity | 1 | |
Binding sitei | 281 | FADBy similarity | 1 | |
Binding sitei | 302 | FADBy similarity | 1 | |
Metal bindingi | 316 | Iron (heme axial ligand)By similarity | 1 | |
Binding sitei | 378 | FADBy similarity | 1 | |
Binding sitei | 395 | FADBy similarity | 1 | |
Metal bindingi | 409 | Iron (heme axial ligand)By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 36 – 39 | NADPBy similarity | 4 | |
Nucleotide bindingi | 58 – 59 | NADPBy similarity | 2 | |
Nucleotide bindingi | 91 – 98 | NADPBy similarity | 8 |
GO - Molecular functioni
- cupric reductase activity Source: MGI
- ferric-chelate reductase (NADPH) activity Source: MGI
- ferric-chelate reductase activity Source: MGI
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- cell cycle Source: UniProtKB-KW
- copper ion import Source: MGI
- exosomal secretion Source: MGI
- iron import into cell Source: MGI
- iron ion import across cell outer membrane Source: MGI
- iron ion transport Source: MGI
- positive regulation of apoptotic process Source: MGI
- positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: MGI
- protein secretion Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Apoptosis, Cell cycle, Ion transport, Iron transport, Transport |
Ligand | Copper, FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP |
Enzyme and pathway databases
Reactomei | R-MMU-6803204, TP53 Regulates Transcription of Genes Involved in Cytochrome C Release R-MMU-9013148, CDC42 GTPase cycle R-MMU-9013405, RHOD GTPase cycle R-MMU-9013406, RHOQ GTPase cycle R-MMU-9013409, RHOJ GTPase cycle R-MMU-9035034, RHOF GTPase cycle R-MMU-917977, Transferrin endocytosis and recycling |
Names & Taxonomyi
Protein namesi | Recommended name: Metalloreductase STEAP3 (EC:1.16.1.-)Alternative name(s): Dudulin-2 Protein nm1054 Six-transmembrane epithelial antigen of prostate 3 Tumor suppressor-activated pathway protein 6 |
Gene namesi | Name:Steap3 Synonyms:Tsap6 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915678, Steap3 |
Subcellular locationi
Endosome
- Endosome membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endosome
- endosome Source: MGI
- endosome membrane Source: UniProtKB-SubCell
- multivesicular body Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: MGI
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 207 | CytoplasmicSequence analysisAdd BLAST | 207 | |
Transmembranei | 208 – 228 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 229 – 258 | VesicularSequence analysisAdd BLAST | 30 | |
Transmembranei | 259 – 279 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 280 – 304 | CytoplasmicSequence analysisAdd BLAST | 25 | |
Transmembranei | 305 – 325 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 326 – 358 | VesicularSequence analysisAdd BLAST | 33 | |
Transmembranei | 359 – 379 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 380 – 390 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 391 – 411 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 412 – 433 | VesicularSequence analysisAdd BLAST | 22 | |
Transmembranei | 434 – 454 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 455 – 488 | CytoplasmicSequence analysisAdd BLAST | 34 |
Keywords - Cellular componenti
Endosome, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 316 | H → L: Loss of function. 1 Publication | 1 | |
Mutagenesisi | 409 | H → L: Loss of function. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000285172 | 1 – 488 | Metalloreductase STEAP3Add BLAST | 488 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 11 | PhosphoserineBy similarity | 1 | |
Modified residuei | 17 | PhosphoserineCombined sources | 1 | |
Modified residuei | 20 | PhosphoserineCombined sources | 1 | |
Glycosylationi | 256 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 486 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 325 – 326 | Cleavage; by RHBDL4/RHBDD1By similarity | 2 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | Q8CI59 |
MaxQBi | Q8CI59 |
PaxDbi | Q8CI59 |
PeptideAtlasi | Q8CI59 |
PRIDEi | Q8CI59 |
ProteomicsDBi | 257489 [Q8CI59-1] 257490 [Q8CI59-2] |
PTM databases
GlyGeni | Q8CI59, 1 site |
iPTMneti | Q8CI59 |
PhosphoSitePlusi | Q8CI59 |
SwissPalmi | Q8CI59 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Homodimer.
Interacts with BNIP3L, MYT1, RHBDL4/RHBDD1 and TCTP (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 212849, 3 interactors |
IntActi | Q8CI59, 4 interactors |
STRINGi | 10090.ENSMUSP00000108260 |
Miscellaneous databases
RNActi | Q8CI59, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 259 – 407 | Ferric oxidoreductaseAdd BLAST | 149 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QRP3, Eukaryota |
InParanoidi | Q8CI59 |
PhylomeDBi | Q8CI59 |
TreeFami | TF332031 |
Family and domain databases
InterProi | View protein in InterPro IPR013130, Fe3_Rdtase_TM_dom IPR036291, NAD(P)-bd_dom_sf IPR028939, P5C_Rdtase_cat_N |
Pfami | View protein in Pfam PF03807, F420_oxidored, 1 hit PF01794, Ferric_reduct, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSGEMDKPLI SRRLVDSDGS LAEVPKEAPK VGILGSGDFA RSLATRLVGS
60 70 80 90 100
GFSVVVGSRN PKRTAGLFPS LAQVTFQEEA VSSPEVIFVA VFREHYSSLC
110 120 130 140 150
SLADQLAGKI LVDVSNPTEK EHLQHRQSNA EYLASLFPAC TVVKAFNVIS
160 170 180 190 200
AWALQAGPRD GNRQVLICSD QPEAKRTISE MARAMGFTPL DMGSLASARE
210 220 230 240 250
VEAIPLRLLP SWKVPTLLAL GLFVCFYTYN FIRDVLQPYI RKDENKFYKM
260 270 280 290 300
PLSVVNTTLP CVAYVLLSLV YLPGVLAAAL QLRRGTKYQR FPDWLDHWLQ
310 320 330 340 350
HRKQIGLLSF FFAMLHALYS FCLPLRRSHR YDLVNLAVKQ VLANKSRLWV
360 370 380 390 400
EEEVWRMEIY LSLGVLALGM LSLLAVTSLP SIANSLNWKE FSFVQSTLGF
410 420 430 440 450
VALILSTMHT LTYGWTRAFE ENHYKFYLPP TFTLTLLLPC VIILAKGLFL
460 470 480
LPCLSRRLTK IRRGWEKDGA VKFMLPGDHT QGEKTSHV
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9QN92 | E9QN92_MOUSE | Metalloreductase STEAP3 | Steap3 | 526 | Annotation score: | ||
A0A0R4J1G9 | A0A0R4J1G9_MOUSE | Metalloreductase STEAP3 | Steap3 | 488 | Annotation score: | ||
D3YTP0 | D3YTP0_MOUSE | Metalloreductase STEAP3 | Steap3 | 418 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 81 | V → M in BAC37383 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 157 | G → V in AAK50539 (Ref. 2) Curated | 1 | |
Sequence conflicti | 169 – 172 | SDQP → GNQQ in AAK50539 (Ref. 2) Curated | 4 | |
Sequence conflicti | 176 – 179 | RTIS → QRVM in AAK50539 (Ref. 2) Curated | 4 | |
Sequence conflicti | 212 | W → G in AAK50539 (Ref. 2) Curated | 1 | |
Sequence conflicti | 350 | V → A in BAC37383 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 350 | V → A in BAE39201 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 455 | S → N in BAC37383 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 455 | S → N in BAE39201 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_024832 | 1 | M → MAAEAHRQQGSCPTIPSEGC GKSPEKKGSAADSRPGTAM in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY214462 mRNA Translation: AAO38239.1 AY029586 mRNA Translation: AAK50539.1 Frameshift. AK078769 mRNA Translation: BAC37383.1 AK167028 mRNA Translation: BAE39201.1 AK171237 mRNA Translation: BAE42333.1 BC037435 mRNA Translation: AAH37435.1 |
CCDSi | CCDS48343.1 [Q8CI59-1] |
RefSeqi | NP_001078878.1, NM_001085409.1 NP_573449.2, NM_133186.3 |
Genome annotation databases
GeneIDi | 68428 |
KEGGi | mmu:68428 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY214462 mRNA Translation: AAO38239.1 AY029586 mRNA Translation: AAK50539.1 Frameshift. AK078769 mRNA Translation: BAC37383.1 AK167028 mRNA Translation: BAE39201.1 AK171237 mRNA Translation: BAE42333.1 BC037435 mRNA Translation: AAH37435.1 |
CCDSi | CCDS48343.1 [Q8CI59-1] |
RefSeqi | NP_001078878.1, NM_001085409.1 NP_573449.2, NM_133186.3 |
3D structure databases
SMRi | Q8CI59 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 212849, 3 interactors |
IntActi | Q8CI59, 4 interactors |
STRINGi | 10090.ENSMUSP00000108260 |
PTM databases
GlyGeni | Q8CI59, 1 site |
iPTMneti | Q8CI59 |
PhosphoSitePlusi | Q8CI59 |
SwissPalmi | Q8CI59 |
Proteomic databases
jPOSTi | Q8CI59 |
MaxQBi | Q8CI59 |
PaxDbi | Q8CI59 |
PeptideAtlasi | Q8CI59 |
PRIDEi | Q8CI59 |
ProteomicsDBi | 257489 [Q8CI59-1] 257490 [Q8CI59-2] |
Genome annotation databases
GeneIDi | 68428 |
KEGGi | mmu:68428 |
Organism-specific databases
CTDi | 55240 |
MGIi | MGI:1915678, Steap3 |
Phylogenomic databases
eggNOGi | ENOG502QRP3, Eukaryota |
InParanoidi | Q8CI59 |
PhylomeDBi | Q8CI59 |
TreeFami | TF332031 |
Enzyme and pathway databases
Reactomei | R-MMU-6803204, TP53 Regulates Transcription of Genes Involved in Cytochrome C Release R-MMU-9013148, CDC42 GTPase cycle R-MMU-9013405, RHOD GTPase cycle R-MMU-9013406, RHOQ GTPase cycle R-MMU-9013409, RHOJ GTPase cycle R-MMU-9035034, RHOF GTPase cycle R-MMU-917977, Transferrin endocytosis and recycling |
Miscellaneous databases
BioGRID-ORCSi | 68428, 1 hit in 64 CRISPR screens |
ChiTaRSi | Steap3, mouse |
PROi | PR:Q8CI59 |
RNActi | Q8CI59, protein |
SOURCEi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR013130, Fe3_Rdtase_TM_dom IPR036291, NAD(P)-bd_dom_sf IPR028939, P5C_Rdtase_cat_N |
Pfami | View protein in Pfam PF03807, F420_oxidored, 1 hit PF01794, Ferric_reduct, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | STEA3_MOUSE | |
Accessioni | Q8CI59Primary (citable) accession number: Q8CI59 Secondary accession number(s): Q3TKE4 Q924Z1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families