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Entry version 143 (22 Apr 2020)
Sequence version 1 (01 Mar 2003)
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Protein

Sphingosine kinase 1

Gene

Sphk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol (PubMed:9726979). In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis (PubMed:16118219). Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling (By similarity). In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway (By similarity). Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity (PubMed:27806293, PubMed:28049734). In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA (PubMed:25417698).By similarity1 Publication5 Publications
Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Acetyltransferase activity increases in presence of the kinase substrate, sphingosine (PubMed:29662056). In Purkinje cells, kinase activity on sphingosine increases in presence of VEGFA (PubMed:25417698). In neurons, kinase activity increases during the first 24h in presence of Amyloid-beta protein 42 to decrease after 96h (By similarity).By similarity2 Publications

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

Kcat=0.0185 min(-1) for Acetyl-CoA as substrate.1 Publication
  1. KM=60.2 µM for Acetyl-CoA (in presence of 10 µM of sphingosine)1 Publication
  2. KM=6.4 µM for Acetyl-CoA (in presence of 100 µM of sphingosine)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei81Proton donor/acceptorBy similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei86ATPPROSITE-ProRule annotation1
    Binding sitei178SubstrateBy similarity1
    Binding sitei185ATPPROSITE-ProRule annotation1
    Binding sitei191ATPPROSITE-ProRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi22 – 24ATPPROSITE-ProRule annotation3
    Nucleotide bindingi54 – 58ATPPROSITE-ProRule annotation5
    Nucleotide bindingi111 – 113ATPPROSITE-ProRule annotation3
    Nucleotide bindingi340 – 342ATPPROSITE-ProRule annotation3

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionCalmodulin-binding, Kinase, Transferase
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDA Comprehensive Enzyme Information System

    More...
    BRENDAi
    2.7.1.91 3474

    Reactome - a knowledgebase of biological pathways and processes

    More...
    Reactomei
    R-MMU-1660661 Sphingolipid de novo biosynthesis
    R-MMU-390471 Association of TriC/CCT with target proteins during biosynthesis
    R-MMU-5218921 VEGFR2 mediated cell proliferation
    R-MMU-9009391 Extra-nuclear estrogen signaling

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...
    SABIO-RKi
    Q8CI15

    Chemistry databases

    SwissLipids knowledge resource for lipid biology

    More...
    SwissLipidsi
    SLP:000000114

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Sphingosine kinase 1Curated (EC:2.7.1.912 Publications)
    Short name:
    SK 11 Publication
    Short name:
    SPK 1
    Alternative name(s):
    Acetyltransferase SPHK11 Publication (EC:2.3.1.-1 Publication)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:Sphk1Imported
    Synonyms:Sk11 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 11

    Organism-specific databases

    Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

    More...
    MGIi
    MGI:1316649 Sphk1

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasm, Endosome, Membrane, Nucleus, Synapse

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

    Mutants are viable, fertile and without any obvious abnormalities (PubMed:15459201). Mutant mice show reduced SPP levels in serum (PubMed:16314531, PubMed:15459201). Conditional mutants in neurons exhibit a decrease of amyloid-beta phagocytic activity (PubMed:29662056). Double knockout for SPHK1 and SPHK2 causes embryonic lethality (PubMed:16314531). Between 11.5 dpc and 12.5 dpc embryos exhibit cranial hemorrhage and die at 13.5 dpc (PubMed:16314531). At 11.5 dpc the wall of the dorsal aorta is poorly developed and endothelial cells are severely defective in all blood vessels in the mesenchymal region of the head (PubMed:16314531). Double knockout embryos also show a neural tube deffect (PubMed:16314531).3 Publications

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi82G → D: No effect on recruitment to endocytic membrane. No effect on membrane fusion produced by sphingosine. 1 Publication1

    Chemistry databases

    ChEMBL database of bioactive drug-like small molecules

    More...
    ChEMBLi
    CHEMBL2401605

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003330321 – 382Sphingosine kinase 1Add BLAST382

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei193PhosphothreonineBy similarity1
    Modified residuei225PhosphoserineBy similarity1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    Q8CI15

    PRoteomics IDEntifications database

    More...
    PRIDEi
    Q8CI15

    PTM databases

    iPTMnet integrated resource for PTMs in systems biology context

    More...
    iPTMneti
    Q8CI15

    Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

    More...
    PhosphoSitePlusi
    Q8CI15

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

    Widely expressed (PubMed:9726979). Expressed in brain (at protein level). Detected in neurons.2 Publications

    <p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

    At 10.5 dpc, expressed in the whole brain, with the highest levels in the telencephalon.1 Publication

    Gene expression databases

    Bgee dataBase for Gene Expression Evolution

    More...
    Bgeei
    ENSMUSG00000061878 Expressed in decidua and 153 other tissues

    ExpressionAtlas, Differential and Baseline Expression

    More...
    ExpressionAtlasi
    Q8CI15 baseline and differential

    Genevisible search portal to normalized and curated expression data from Genevestigator

    More...
    Genevisiblei
    Q8CI15 MM

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Interacts with ACY1 (PubMed:15196915). Binds to calmodulin.

    Interacts with SPHKAP (By similarity).

    Interacts with CIB1, the interaction occurs in a calcium-dependent manner (By similarity).

    Interacts with TRAF2 (By similarity).

    Interacts with EEF1A1; the interaction enhances SPHK1 kinase activity (By similarity).

    By similarity1 Publication

    GO - Molecular functioni

    Protein-protein interaction databases

    Protein interaction database and analysis system

    More...
    IntActi
    Q8CI15, 3 interactors

    STRING: functional protein association networks

    More...
    STRINGi
    10090.ENSMUSP00000131010

    Chemistry databases

    BindingDB database of measured binding affinities

    More...
    BindingDBi
    Q8CI15

    Miscellaneous databases

    RNAct, Protein-RNA interaction predictions for model organisms.

    More...
    RNActi
    Q8CI15 protein

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    Q8CI15

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini12 – 159DAGKcPROSITE-ProRule annotationAdd BLAST148

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni79 – 82Substrate bindingBy similarity4

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi147 – 155Nuclear export signal 1By similarity9
    Motifi161 – 169Nuclear export signal 2By similarity9

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    KOG1116 Eukaryota
    COG1597 LUCA

    Ensembl GeneTree

    More...
    GeneTreei
    ENSGT00940000157864

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    Q8CI15

    KEGG Orthology (KO)

    More...
    KOi
    K04718

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    GNMAIMS

    Database of Orthologous Groups

    More...
    OrthoDBi
    681139at2759

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    Q8CI15

    TreeFam database of animal gene trees

    More...
    TreeFami
    TF354296

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    3.40.50.10330, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR017438 ATP-NAD_kinase_N
    IPR001206 Diacylglycerol_kinase_cat_dom
    IPR016064 NAD/diacylglycerol_kinase_sf

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00781 DAGK_cat, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00046 DAGKc, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF111331 SSF111331, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS50146 DAGK, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

    This entry has 3 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

    Isoform 1 (identifier: Q8CI15-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MEPVECPRGL LPRPCRVLVL LNPQGGKGKA LQLFQSRVQP FLEEAEITFK
    60 70 80 90 100
    LILTERKNHA RELVCAEELG HWDALAVMSG DGLMHEVVNG LMERPDWETA
    110 120 130 140 150
    IQKPLCSLPG GSGNALAASV NHYAGYEQVT NEDLLINCTL LLCRRRLSPM
    160 170 180 190 200
    NLLSLHTASG LRLYSVLSLS WGFVADVDLE SEKYRRLGEI RFTVGTFFRL
    210 220 230 240 250
    ASLRIYQGQL AYLPVGTVAS KRPASTLVQK GPVDTHLVPL EEPVPSHWTV
    260 270 280 290 300
    VPEQDFVLVL VLLHTHLSSE LFAAPMGRCE AGVMHLFYVR AGVSRAALLR
    310 320 330 340 350
    LFLAMQKGKH MELDCPYLVH VPVVAFRLEP RSQRGVFSVD GELMVCEAVQ
    360 370 380
    GQVHPNYLWM VCGSRDAPSG RDSRRGPPPE EP
    Length:382
    Mass (Da):42,443
    Last modified:March 1, 2003 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB791FAA58FCE3D29
    GO
    Isoform 2 (identifier: Q8CI15-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-3: MEP → MWWCCVLFV

    Show »
    Length:388
    Mass (Da):43,254
    Checksum:i01EB032322542CAD
    GO
    Isoform 3 (identifier: Q8CI15-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         4-4: Missing.

    Show »
    Length:381
    Mass (Da):42,344
    Checksum:i2F2C1F10D59EB129
    GO

    <p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

    There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    B1AT89B1AT89_MOUSE
    Sphingosine kinase 1
    Sphk1
    251Annotation score:

    Annotation score:1 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    B1AT87B1AT87_MOUSE
    Sphingosine kinase 1
    Sphk1
    153Annotation score:

    Annotation score:1 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    B1AT88B1AT88_MOUSE
    Sphingosine kinase 1
    Sphk1
    157Annotation score:

    Annotation score:1 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    B1AT85B1AT85_MOUSE
    Sphingosine kinase 1
    Sphk1
    116Annotation score:

    Annotation score:1 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    B1AT86B1AT86_MOUSE
    Sphingosine kinase 1
    Sphk1
    153Annotation score:

    Annotation score:1 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti146R → H in BAE33198 (PubMed:9726979).Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0334481 – 3MEP → MWWCCVLFV in isoform 2. 1 Publication3
    Alternative sequenceiVSP_0334494Missing in isoform 3. 1 Publication1

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AF068749 mRNA Translation: AAC61698.1
    AF415213 mRNA Translation: AAL07499.1
    AK155332 mRNA Translation: BAE33198.1
    AK160900 mRNA Translation: BAE36079.1
    AL645851 Genomic DNA No translation available.
    BC037710 mRNA Translation: AAH37710.1

    The Consensus CDS (CCDS) project

    More...
    CCDSi
    CCDS25668.1 [Q8CI15-1]
    CCDS48986.1 [Q8CI15-3]

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_001165943.1, NM_001172472.1 [Q8CI15-1]
    NP_001165944.1, NM_001172473.1 [Q8CI15-1]
    NP_001165946.1, NM_001172475.1 [Q8CI15-3]
    NP_035581.1, NM_011451.3
    NP_079643.2, NM_025367.6 [Q8CI15-1]
    XP_006532733.1, XM_006532670.3
    XP_006532734.1, XM_006532671.3
    XP_006532735.1, XM_006532672.3 [Q8CI15-3]
    XP_006532736.1, XM_006532673.3

    Genome annotation databases

    Ensembl eukaryotic genome annotation project

    More...
    Ensembli
    ENSMUST00000063396; ENSMUSP00000064743; ENSMUSG00000061878 [Q8CI15-1]
    ENSMUST00000063446; ENSMUSP00000067865; ENSMUSG00000061878 [Q8CI15-1]
    ENSMUST00000100201; ENSMUSP00000097775; ENSMUSG00000061878 [Q8CI15-3]
    ENSMUST00000106386; ENSMUSP00000101994; ENSMUSG00000061878 [Q8CI15-1]
    ENSMUST00000106387; ENSMUSP00000101995; ENSMUSG00000061878 [Q8CI15-1]
    ENSMUST00000106388; ENSMUSP00000101996; ENSMUSG00000061878 [Q8CI15-1]
    ENSMUST00000141798; ENSMUSP00000131010; ENSMUSG00000061878 [Q8CI15-2]

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    20698

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    mmu:20698

    UCSC genome browser

    More...
    UCSCi
    uc007mlg.2 mouse [Q8CI15-3]
    uc007mlh.2 mouse [Q8CI15-1]
    uc007mll.2 mouse [Q8CI15-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF068749 mRNA Translation: AAC61698.1
    AF415213 mRNA Translation: AAL07499.1
    AK155332 mRNA Translation: BAE33198.1
    AK160900 mRNA Translation: BAE36079.1
    AL645851 Genomic DNA No translation available.
    BC037710 mRNA Translation: AAH37710.1
    CCDSiCCDS25668.1 [Q8CI15-1]
    CCDS48986.1 [Q8CI15-3]
    RefSeqiNP_001165943.1, NM_001172472.1 [Q8CI15-1]
    NP_001165944.1, NM_001172473.1 [Q8CI15-1]
    NP_001165946.1, NM_001172475.1 [Q8CI15-3]
    NP_035581.1, NM_011451.3
    NP_079643.2, NM_025367.6 [Q8CI15-1]
    XP_006532733.1, XM_006532670.3
    XP_006532734.1, XM_006532671.3
    XP_006532735.1, XM_006532672.3 [Q8CI15-3]
    XP_006532736.1, XM_006532673.3

    3D structure databases

    SMRiQ8CI15
    ModBaseiSearch...

    Protein-protein interaction databases

    IntActiQ8CI15, 3 interactors
    STRINGi10090.ENSMUSP00000131010

    Chemistry databases

    BindingDBiQ8CI15
    ChEMBLiCHEMBL2401605
    SwissLipidsiSLP:000000114

    PTM databases

    iPTMnetiQ8CI15
    PhosphoSitePlusiQ8CI15

    Proteomic databases

    PaxDbiQ8CI15
    PRIDEiQ8CI15

    Protocols and materials databases

    Antibodypedia a portal for validated antibodies

    More...
    Antibodypediai
    19687 700 antibodies

    Genome annotation databases

    EnsembliENSMUST00000063396; ENSMUSP00000064743; ENSMUSG00000061878 [Q8CI15-1]
    ENSMUST00000063446; ENSMUSP00000067865; ENSMUSG00000061878 [Q8CI15-1]
    ENSMUST00000100201; ENSMUSP00000097775; ENSMUSG00000061878 [Q8CI15-3]
    ENSMUST00000106386; ENSMUSP00000101994; ENSMUSG00000061878 [Q8CI15-1]
    ENSMUST00000106387; ENSMUSP00000101995; ENSMUSG00000061878 [Q8CI15-1]
    ENSMUST00000106388; ENSMUSP00000101996; ENSMUSG00000061878 [Q8CI15-1]
    ENSMUST00000141798; ENSMUSP00000131010; ENSMUSG00000061878 [Q8CI15-2]
    GeneIDi20698
    KEGGimmu:20698
    UCSCiuc007mlg.2 mouse [Q8CI15-3]
    uc007mlh.2 mouse [Q8CI15-1]
    uc007mll.2 mouse [Q8CI15-2]

    Organism-specific databases

    Comparative Toxicogenomics Database

    More...
    CTDi
    8877
    MGIiMGI:1316649 Sphk1

    Phylogenomic databases

    eggNOGiKOG1116 Eukaryota
    COG1597 LUCA
    GeneTreeiENSGT00940000157864
    InParanoidiQ8CI15
    KOiK04718
    OMAiGNMAIMS
    OrthoDBi681139at2759
    PhylomeDBiQ8CI15
    TreeFamiTF354296

    Enzyme and pathway databases

    BRENDAi2.7.1.91 3474
    ReactomeiR-MMU-1660661 Sphingolipid de novo biosynthesis
    R-MMU-390471 Association of TriC/CCT with target proteins during biosynthesis
    R-MMU-5218921 VEGFR2 mediated cell proliferation
    R-MMU-9009391 Extra-nuclear estrogen signaling
    SABIO-RKiQ8CI15

    Miscellaneous databases

    ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

    More...
    ChiTaRSi
    Sphk1 mouse

    Protein Ontology

    More...
    PROi
    PR:Q8CI15
    RNActiQ8CI15 protein

    The Stanford Online Universal Resource for Clones and ESTs

    More...
    SOURCEi
    Search...

    Gene expression databases

    BgeeiENSMUSG00000061878 Expressed in decidua and 153 other tissues
    ExpressionAtlasiQ8CI15 baseline and differential
    GenevisibleiQ8CI15 MM

    Family and domain databases

    Gene3Di3.40.50.10330, 1 hit
    InterProiView protein in InterPro
    IPR017438 ATP-NAD_kinase_N
    IPR001206 Diacylglycerol_kinase_cat_dom
    IPR016064 NAD/diacylglycerol_kinase_sf
    PfamiView protein in Pfam
    PF00781 DAGK_cat, 1 hit
    SMARTiView protein in SMART
    SM00046 DAGKc, 1 hit
    SUPFAMiSSF111331 SSF111331, 1 hit
    PROSITEiView protein in PROSITE
    PS50146 DAGK, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSPHK1_MOUSE
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8CI15
    Secondary accession number(s): O88886, Q3U2E3, Q91ZN3
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: March 1, 2003
    Last modified: April 22, 2020
    This is version 143 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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