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Protein

Translation initiation factor eIF-2B subunit epsilon

Gene

Eif2b5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-72731 Recycling of eIF2:GDP

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor eIF-2B subunit epsilon
Alternative name(s):
eIF-2B GDP-GTP exchange factor subunit epsilon
Gene namesi
Name:Eif2b5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:2446176 Eif2b5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002394291 – 717Translation initiation factor eIF-2B subunit epsilonAdd BLAST717

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18Omega-N-methylarginineCombined sources1
Modified residuei23PhosphoserineBy similarity1
Cross-linki57Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki99Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei126PhosphoserineBy similarity1
Cross-linki137Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki213Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei318PhosphothreonineBy similarity1
Modified residuei446PhosphoserineBy similarity1
Modified residuei462PhosphoserineBy similarity1
Modified residuei465PhosphoserineBy similarity1
Cross-linki501Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei528PhosphoserineBy similarity1
Modified residuei536PhosphoserineBy similarity1
Modified residuei540Phosphoserine; by DYRK2Combined sources1
Modified residuei713PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated at Ser-540 by DYRK2; this is required for subsequent phosphorylation by GSK3B. Phosphorylated on serine and threonine residues by GSK3B; phosphorylation inhibits its function (By similarity).By similarity
Polyubiquitinated, probably by NEDD4.By similarity

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8CHW4
MaxQBiQ8CHW4
PaxDbiQ8CHW4
PeptideAtlasiQ8CHW4
PRIDEiQ8CHW4

PTM databases

iPTMnetiQ8CHW4
PhosphoSitePlusiQ8CHW4
SwissPalmiQ8CHW4

Expressioni

Gene expression databases

BgeeiENSMUSG00000003235 Expressed in 292 organ(s), highest expression level in embryo
ExpressionAtlasiQ8CHW4 baseline and differential
GenevisibleiQ8CHW4 MM

Interactioni

Subunit structurei

Complex of five different subunits; alpha, beta, gamma, delta and epsilon. Interacts with RGS2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003320

Structurei

3D structure databases

ProteinModelPortaliQ8CHW4
SMRiQ8CHW4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini539 – 716W2PROSITE-ProRule annotationAdd BLAST178

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1461 Eukaryota
COG1208 LUCA
GeneTreeiENSGT00510000047568
HOGENOMiHOG000216610
HOVERGENiHBG051460
InParanoidiQ8CHW4
KOiK03240
OMAiPQMDDIK
OrthoDBiEOG091G065U
PhylomeDBiQ8CHW4
TreeFamiTF101509

Family and domain databases

CDDicd04197 eIF-2B_epsilon_N, 1 hit
Gene3Di1.25.40.180, 1 hit
3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR035543 eIF-2B_epsilon_N
IPR001451 Hexapep
IPR016021 MIF4-like_sf
IPR029044 Nucleotide-diphossugar_trans
IPR011004 Trimer_LpxA-like_sf
IPR003307 W2_domain
PfamiView protein in Pfam
PF00132 Hexapep, 1 hit
PF02020 W2, 1 hit
SMARTiView protein in SMART
SM00515 eIF5C, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
SSF51161 SSF51161, 1 hit
SSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS51363 W2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q8CHW4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAATAAVPGA AAGRASKRGG GGSGGGGTQG AEEEPPPPLQ AVLVADSFNR
60 70 80 90 100
RFFPISKDQP RVLLPLANVA LIDYTLEFLT ATGVQETFVF CCWKAAQIKE
110 120 130 140 150
HLQKSKWCHP TSPNVVRIIT SELYRSLGDV LRDVDAKALV RSDFLLIYGD
160 170 180 190 200
VISNINICRA LEEHRLRRKL EKNVSVMTMV FKESSPSHPT RCHEDNVVMA
210 220 230 240 250
VDSATNRVLH FQKTQGLRRF SFPLSLFQGS GDGVEIRYDL LDCHISICSP
260 270 280 290 300
QVAQLFTDNF DYQTRDDFVR GILMNEEVLG NQIHLHVTTR EYGARVSNLH
310 320 330 340 350
MYSAVCADVI RRWVYPLTPE VNFTDSTTQS YTHSRHNIYR GPEVSLGHGS
360 370 380 390 400
VLEENVLLGA GTVIGSNCSI TNSVIGPNCH IGDNVVLDQA YLWQGVRVAA
410 420 430 440 450
GAQIHQSLLC DRAEVKERVK LKPYCVLTSQ VVVGPDITLP EGSVISLHPP
460 470 480 490 500
DAEEDEDDGQ FSDDSGADQE KEKVKLKGYN PAEVGLEGQG YLWKAEGVNS
510 520 530 540 550
KEDEELRQSL WGLMIKTEEE SETESEGSVD PEELDSRAGS PQLDDIRVFQ
560 570 580 590 600
NEVLGTLQRG REENISCENL VLEINSLKHA YNISLKEVMQ VLTLVVLEFP
610 620 630 640 650
LQQVDGLLDP NRYCALLLPL LKAWSPVLRN YIKRAADHLE ALAAIEDFFL
660 670 680 690 700
EHETLVTSMA KVLMAFYQLE ILAEETILSW FSQRDTTDEG QQLRKNQQLQ
710
RFIQWLREAE EESSEDD
Length:717
Mass (Da):80,086
Last modified:March 1, 2003 - v1
Checksum:i61455A437B7D513B
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6T9C3F6T9C3_MOUSE
Translation initiation factor eIF-2...
Eif2b5
160Annotation score:
A0A338P6D9A0A338P6D9_MOUSE
Translation initiation factor eIF-2...
Eif2b5
303Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54P → H in BAE36132 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK160985 mRNA Translation: BAE36132.1
BC038620 mRNA Translation: AAH38620.1
BC085255 mRNA Translation: AAH85255.1
CCDSiCCDS28047.1
RefSeqiNP_758469.1, NM_172265.2
UniGeneiMm.233855

Genome annotation databases

EnsembliENSMUST00000003320; ENSMUSP00000003320; ENSMUSG00000003235
GeneIDi224045
KEGGimmu:224045
UCSCiuc007ypu.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK160985 mRNA Translation: BAE36132.1
BC038620 mRNA Translation: AAH38620.1
BC085255 mRNA Translation: AAH85255.1
CCDSiCCDS28047.1
RefSeqiNP_758469.1, NM_172265.2
UniGeneiMm.233855

3D structure databases

ProteinModelPortaliQ8CHW4
SMRiQ8CHW4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003320

PTM databases

iPTMnetiQ8CHW4
PhosphoSitePlusiQ8CHW4
SwissPalmiQ8CHW4

Proteomic databases

EPDiQ8CHW4
MaxQBiQ8CHW4
PaxDbiQ8CHW4
PeptideAtlasiQ8CHW4
PRIDEiQ8CHW4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003320; ENSMUSP00000003320; ENSMUSG00000003235
GeneIDi224045
KEGGimmu:224045
UCSCiuc007ypu.2 mouse

Organism-specific databases

CTDi8893
MGIiMGI:2446176 Eif2b5

Phylogenomic databases

eggNOGiKOG1461 Eukaryota
COG1208 LUCA
GeneTreeiENSGT00510000047568
HOGENOMiHOG000216610
HOVERGENiHBG051460
InParanoidiQ8CHW4
KOiK03240
OMAiPQMDDIK
OrthoDBiEOG091G065U
PhylomeDBiQ8CHW4
TreeFamiTF101509

Enzyme and pathway databases

ReactomeiR-MMU-72731 Recycling of eIF2:GDP

Miscellaneous databases

PROiPR:Q8CHW4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003235 Expressed in 292 organ(s), highest expression level in embryo
ExpressionAtlasiQ8CHW4 baseline and differential
GenevisibleiQ8CHW4 MM

Family and domain databases

CDDicd04197 eIF-2B_epsilon_N, 1 hit
Gene3Di1.25.40.180, 1 hit
3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR035543 eIF-2B_epsilon_N
IPR001451 Hexapep
IPR016021 MIF4-like_sf
IPR029044 Nucleotide-diphossugar_trans
IPR011004 Trimer_LpxA-like_sf
IPR003307 W2_domain
PfamiView protein in Pfam
PF00132 Hexapep, 1 hit
PF02020 W2, 1 hit
SMARTiView protein in SMART
SM00515 eIF5C, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
SSF51161 SSF51161, 1 hit
SSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS51363 W2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEI2BE_MOUSE
AccessioniPrimary (citable) accession number: Q8CHW4
Secondary accession number(s): Q3TU39
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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