UniProtKB - Q8CHW4 (EI2BE_MOUSE)

BLAST

>sp|Q8CHW4|EI2BE_MOUSE Translation initiation factor eIF-2B subunit epsilon OS=Mus musculus OX=10090 GN=Eif2b5 PE=1 SV=1
MAATAAVPGAAAGRASKRGGGGSGGGGTQGAEEEPPPPLQAVLVADSFNRRFFPISKDQP
RVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLQKSKWCHPTSPNVVRIIT
SELYRSLGDVLRDVDAKALVRSDFLLIYGDVISNINICRALEEHRLRRKLEKNVSVMTMV
FKESSPSHPTRCHEDNVVMAVDSATNRVLHFQKTQGLRRFSFPLSLFQGSGDGVEIRYDL
LDCHISICSPQVAQLFTDNFDYQTRDDFVRGILMNEEVLGNQIHLHVTTREYGARVSNLH
MYSAVCADVIRRWVYPLTPEVNFTDSTTQSYTHSRHNIYRGPEVSLGHGSVLEENVLLGA
GTVIGSNCSITNSVIGPNCHIGDNVVLDQAYLWQGVRVAAGAQIHQSLLCDRAEVKERVK
LKPYCVLTSQVVVGPDITLPEGSVISLHPPDAEEDEDDGQFSDDSGADQEKEKVKLKGYN
PAEVGLEGQGYLWKAEGVNSKEDEELRQSLWGLMIKTEEESETESEGSVDPEELDSRAGS
PQLDDIRVFQNEVLGTLQRGREENISCENLVLEINSLKHAYNISLKEVMQVLTLVVLEFP
LQQVDGLLDPNRYCALLLPLLKAWSPVLRNYIKRAADHLEALAAIEDFFLEHETLVTSMA
KVLMAFYQLEILAEETILSWFSQRDTTDEGQQLRKNQQLQRFIQWLREAEEESSEDD

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History
Entry version 121 (07 Nov 2018)
Sequence version 1 (01 Mar 2003)
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Protein

Translation initiation factor eIF-2B subunit epsilon

Gene

Eif2b5

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.By similarity

GO - Molecular functionⁱ

guanyl-nucleotide exchange factor activity Source: MGI
translation initiation factor activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
translation initiation factor binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

aging Source: MGI
astrocyte development Source: UniProtKB
astrocyte differentiation Source: UniProtKB
hippocampus development Source: Ensembl
myelination Source: UniProtKB
oligodendrocyte development Source: UniProtKB
ovarian follicle development Source: UniProtKB
positive regulation of apoptotic process Source: MGI
positive regulation of translation Source: MGI
positive regulation of translational initiation Source: UniProtKB
response to endoplasmic reticulum stress Source: UniProtKB
response to glucose Source: UniProtKB
response to heat Source: UniProtKB
response to lithium ion Source: Ensembl
response to peptide hormone Source: UniProtKB
T cell receptor signaling pathway Source: UniProtKB
translational initiation Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Initiation factor
Biological process	Protein biosynthesis

Enzyme and pathway databases

Reactomeⁱ	R-MMU-72731 Recycling of eIF2:GDP

Reactomeⁱ

R-MMU-72731 Recycling of eIF2:GDP

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Translation initiation factor eIF-2B subunit epsilon Alternative name(s): eIF-2B GDP-GTP exchange factor subunit epsilon
Gene namesⁱ	Name:Eif2b5
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 16

Organism-specific databases

MGIⁱ	MGI:2446176 Eif2b5

Subcellular locationⁱ

GO - Cellular component

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000239429	1 – 717	Translation initiation factor eIF-2B subunit epsilonAdd BLAST		717

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	18	Omega-N-methylarginineCombined sources	1
Modified residueⁱ	23	PhosphoserineBy similarity	1
Cross-linkⁱ	57	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linkⁱ	99	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residueⁱ	126	PhosphoserineBy similarity	1
Cross-linkⁱ	137	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linkⁱ	213	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residueⁱ	318	PhosphothreonineBy similarity	1
Modified residueⁱ	446	PhosphoserineBy similarity	1
Modified residueⁱ	462	PhosphoserineBy similarity	1
Modified residueⁱ	465	PhosphoserineBy similarity	1
Cross-linkⁱ	501	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residueⁱ	528	PhosphoserineBy similarity	1
Modified residueⁱ	536	PhosphoserineBy similarity	1
Modified residueⁱ	540	Phosphoserine; by DYRK2Combined sources	1
Modified residueⁱ	713	PhosphoserineBy similarity	1

Post-translational modificationⁱ

Phosphorylated at Ser-540 by DYRK2; this is required for subsequent phosphorylation by GSK3B. Phosphorylated on serine and threonine residues by GSK3B; phosphorylation inhibits its function (By similarity).By similarity

Polyubiquitinated, probably by NEDD4.By similarity

Keywords - PTMⁱ

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q8CHW4
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q8CHW4
PaxDbⁱ	Q8CHW4
PeptideAtlas More...PeptideAtlasⁱ	Q8CHW4
PRIDEⁱ	Q8CHW4

PTM databases

iPTMnetⁱ	Q8CHW4
PhosphoSitePlusⁱ	Q8CHW4
SwissPalmⁱ	Q8CHW4

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000003235 Expressed in 292 organ(s), highest expression level in embryo
ExpressionAtlasⁱ	Q8CHW4 baseline and differential
Genevisibleⁱ	Q8CHW4 MM

Interactionⁱ

Subunit structureⁱ

Complex of five different subunits; alpha, beta, gamma, delta and epsilon. Interacts with RGS2 (By similarity).By similarity

GO - Molecular functionⁱ

guanyl-nucleotide exchange factor activity Source: MGI
translation initiation factor binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000003320

STRINGⁱ

10090.ENSMUSP00000003320

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8CHW4
SMRⁱ	Q8CHW4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	539 – 716	W2PROSITE-ProRule annotationAdd BLAST		178

Sequence similaritiesⁱ

Belongs to the eIF-2B gamma/epsilon subunits family.Curated

Phylogenomic databases

eggNOGⁱ	KOG1461 Eukaryota COG1208 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000047568
HOGENOMⁱ	HOG000216610
HOVERGENⁱ	HBG051460
InParanoidⁱ	Q8CHW4
KEGG Orthology (KO) More...KOⁱ	K03240
OMAⁱ	PQMDDIK
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G065U
PhylomeDBⁱ	Q8CHW4
TreeFamⁱ	TF101509

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd04197 eIF-2B_epsilon_N, 1 hit
Gene3Dⁱ	1.25.40.180, 1 hit 3.90.550.10, 1 hit
InterProⁱ	View protein in InterPro IPR016024 ARM-type_fold IPR035543 eIF-2B_epsilon_N IPR001451 Hexapep IPR016021 MIF4-like_sf IPR029044 Nucleotide-diphossugar_trans IPR011004 Trimer_LpxA-like_sf IPR003307 W2_domain
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00132 Hexapep, 1 hit PF02020 W2, 1 hit
SMARTⁱ	View protein in SMART SM00515 eIF5C, 1 hit
SUPFAMⁱ	SSF48371 SSF48371, 1 hit SSF51161 SSF51161, 1 hit SSF53448 SSF53448, 1 hit
PROSITEⁱ	View protein in PROSITE PS51363 W2, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show all Align All

Q8CHW4-1 [UniParc]FASTA Add to basket

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        10         20         30         40         50
MAATAAVPGA AAGRASKRGG GGSGGGGTQG AEEEPPPPLQ AVLVADSFNR 
        60         70         80         90        100
RFFPISKDQP RVLLPLANVA LIDYTLEFLT ATGVQETFVF CCWKAAQIKE 
       110        120        130        140        150
HLQKSKWCHP TSPNVVRIIT SELYRSLGDV LRDVDAKALV RSDFLLIYGD 
       160        170        180        190        200
VISNINICRA LEEHRLRRKL EKNVSVMTMV FKESSPSHPT RCHEDNVVMA 
       210        220        230        240        250
VDSATNRVLH FQKTQGLRRF SFPLSLFQGS GDGVEIRYDL LDCHISICSP 
       260        270        280        290        300
QVAQLFTDNF DYQTRDDFVR GILMNEEVLG NQIHLHVTTR EYGARVSNLH 
       310        320        330        340        350
MYSAVCADVI RRWVYPLTPE VNFTDSTTQS YTHSRHNIYR GPEVSLGHGS 
       360        370        380        390        400
VLEENVLLGA GTVIGSNCSI TNSVIGPNCH IGDNVVLDQA YLWQGVRVAA 
       410        420        430        440        450
GAQIHQSLLC DRAEVKERVK LKPYCVLTSQ VVVGPDITLP EGSVISLHPP 
       460        470        480        490        500
DAEEDEDDGQ FSDDSGADQE KEKVKLKGYN PAEVGLEGQG YLWKAEGVNS 
       510        520        530        540        550
KEDEELRQSL WGLMIKTEEE SETESEGSVD PEELDSRAGS PQLDDIRVFQ 
       560        570        580        590        600
NEVLGTLQRG REENISCENL VLEINSLKHA YNISLKEVMQ VLTLVVLEFP 
       610        620        630        640        650
LQQVDGLLDP NRYCALLLPL LKAWSPVLRN YIKRAADHLE ALAAIEDFFL 
       660        670        680        690        700
EHETLVTSMA KVLMAFYQLE ILAEETILSW FSQRDTTDEG QQLRKNQQLQ 
       710 
RFIQWLREAE EESSEDD

Length:717

Mass (Da):80,086

Last modified:March 1, 2003 - v1

Checksum:ⁱ61455A437B7D513B

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

F6T9C3	F6T9C3_MOUSE	Translation initiation factor eIF-2... Translation initiation factor eIF-2B subunit epsilon	Eif2b5	160	Annotation score:
A0A338P6D9	A0A338P6D9_MOUSE	Translation initiation factor eIF-2... Translation initiation factor eIF-2B subunit epsilon	Eif2b5	303	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	54	P → H in BAE36132 (PubMed:16141072).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK160985 mRNA Translation: BAE36132.1 BC038620 mRNA Translation: AAH38620.1 BC085255 mRNA Translation: AAH85255.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS28047.1
NCBI Reference Sequences More...RefSeqⁱ	NP_758469.1, NM_172265.2
UniGeneⁱ	Mm.233855

Genome annotation databases

Ensemblⁱ	ENSMUST00000003320; ENSMUSP00000003320; ENSMUSG00000003235
GeneIDⁱ	224045
KEGGⁱ	mmu:224045
UCSC genome browser More...UCSCⁱ	uc007ypu.2 mouse

Protein	Similar proteins		Species	Score	Length	Source
Q8CHW4	Translation initiation factor eIF-2B subunit epsilon		RAT		716	UniRef90_Q64350
translation initiation factor eIF-2B subunit epsilon		MESAU		716
UPI00038BDCC2		MICOH		717
UPI000A317204		MUSCR		716
UPI000B4F05E1		MERUN		716

Protein	Similar proteins		Species	Score	Length	Source
Q8CHW4	Translation initiation factor eIF-2B subunit epsilon	)	HUMAN		721	UniRef50_Q13144
Translation initiation factor eIF-2B subunit epsilon		RABIT		721
Translation initiation factor eIF-2B subunit epsilon		RAT		716
translation initiation factor eIF-2B subunit epsilon		TARSY		734
translation initiation factor eIF-2B subunit epsilon		Yangtze finless porpoise		728
+336

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK160985 mRNA Translation: BAE36132.1 BC038620 mRNA Translation: AAH38620.1 BC085255 mRNA Translation: AAH85255.1
CCDSⁱ	CCDS28047.1
RefSeqⁱ	NP_758469.1, NM_172265.2
UniGeneⁱ	Mm.233855

3D structure databases

ProteinModelPortalⁱ	Q8CHW4
SMRⁱ	Q8CHW4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000003320

STRINGⁱ

10090.ENSMUSP00000003320

PTM databases

iPTMnetⁱ	Q8CHW4
PhosphoSitePlusⁱ	Q8CHW4
SwissPalmⁱ	Q8CHW4

Proteomic databases

EPDⁱ	Q8CHW4
MaxQBⁱ	Q8CHW4
PaxDbⁱ	Q8CHW4
PeptideAtlasⁱ	Q8CHW4
PRIDEⁱ	Q8CHW4

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000003320; ENSMUSP00000003320; ENSMUSG00000003235
GeneIDⁱ	224045
KEGGⁱ	mmu:224045
UCSCⁱ	uc007ypu.2 mouse

Organism-specific databases

CTDⁱ	8893
MGIⁱ	MGI:2446176 Eif2b5

Phylogenomic databases

eggNOGⁱ	KOG1461 Eukaryota COG1208 LUCA
GeneTreeⁱ	ENSGT00510000047568
HOGENOMⁱ	HOG000216610
HOVERGENⁱ	HBG051460
InParanoidⁱ	Q8CHW4
KOⁱ	K03240
OMAⁱ	PQMDDIK
OrthoDBⁱ	EOG091G065U
PhylomeDBⁱ	Q8CHW4
TreeFamⁱ	TF101509

Enzyme and pathway databases

Reactomeⁱ	R-MMU-72731 Recycling of eIF2:GDP

Reactomeⁱ

R-MMU-72731 Recycling of eIF2:GDP

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q8CHW4
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000003235 Expressed in 292 organ(s), highest expression level in embryo
ExpressionAtlasⁱ	Q8CHW4 baseline and differential
Genevisibleⁱ	Q8CHW4 MM

Family and domain databases

CDDⁱ	cd04197 eIF-2B_epsilon_N, 1 hit
Gene3Dⁱ	1.25.40.180, 1 hit 3.90.550.10, 1 hit
InterProⁱ	View protein in InterPro IPR016024 ARM-type_fold IPR035543 eIF-2B_epsilon_N IPR001451 Hexapep IPR016021 MIF4-like_sf IPR029044 Nucleotide-diphossugar_trans IPR011004 Trimer_LpxA-like_sf IPR003307 W2_domain
Pfamⁱ	View protein in Pfam PF00132 Hexapep, 1 hit PF02020 W2, 1 hit
SMARTⁱ	View protein in SMART SM00515 eIF5C, 1 hit
SUPFAMⁱ	SSF48371 SSF48371, 1 hit SSF51161 SSF51161, 1 hit SSF53448 SSF53448, 1 hit
PROSITEⁱ	View protein in PROSITE PS51363 W2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	EI2BE_MOUSE
Accessionⁱ	Q8CHW4Primary (citable) accession number: Q8CHW4 Secondary accession number(s): Q3TU39
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
	Last sequence update:	March 1, 2003
	Last modified:	November 7, 2018
	This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8CHW4 (EI2BE_MOUSE)

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Translation initiation factor eIF-2B subunit epsilon

Eif2b5

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Cytosol

Nucleus

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ