UniProtKB - Q8CHW4 (EI2BE_MOUSE)
Protein
Translation initiation factor eIF-2B subunit epsilon
Gene
Eif2b5
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.By similarity
GO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: MGI
- translation initiation factor activity Source: GO_Central
- translation initiation factor binding Source: MGI
GO - Biological processi
- aging Source: MGI
- astrocyte development Source: UniProtKB
- astrocyte differentiation Source: UniProtKB
- hippocampus development Source: Ensembl
- myelination Source: UniProtKB
- oligodendrocyte development Source: UniProtKB
- ovarian follicle development Source: UniProtKB
- positive regulation of apoptotic process Source: MGI
- positive regulation of translation Source: MGI
- positive regulation of translational initiation Source: UniProtKB
- response to endoplasmic reticulum stress Source: UniProtKB
- response to glucose Source: UniProtKB
- response to heat Source: UniProtKB
- response to lithium ion Source: Ensembl
- response to peptide hormone Source: UniProtKB
- T cell receptor signaling pathway Source: UniProtKB
- translational initiation Source: UniProtKB
Keywordsi
Molecular function | Initiation factor |
Biological process | Protein biosynthesis |
Enzyme and pathway databases
Reactomei | R-MMU-72731, Recycling of eIF2:GDP |
Names & Taxonomyi
Protein namesi | Recommended name: Translation initiation factor eIF-2B subunit epsilonAlternative name(s): eIF-2B GDP-GTP exchange factor subunit epsilon |
Gene namesi | Name:Eif2b5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2446176, Eif2b5 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- eukaryotic translation initiation factor 2B complex Source: UniProtKB
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000239429 | 1 – 717 | Translation initiation factor eIF-2B subunit epsilonAdd BLAST | 717 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 18 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 23 | PhosphoserineBy similarity | 1 | |
Cross-linki | 57 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 99 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 126 | PhosphoserineBy similarity | 1 | |
Cross-linki | 137 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 213 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 318 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 446 | PhosphoserineBy similarity | 1 | |
Modified residuei | 462 | PhosphoserineBy similarity | 1 | |
Modified residuei | 465 | PhosphoserineBy similarity | 1 | |
Cross-linki | 501 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 528 | PhosphoserineBy similarity | 1 | |
Modified residuei | 536 | PhosphoserineBy similarity | 1 | |
Modified residuei | 540 | Phosphoserine; by DYRK2Combined sources | 1 | |
Modified residuei | 713 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Phosphorylated at Ser-540 by DYRK2; this is required for subsequent phosphorylation by GSK3B. Phosphorylated on serine and threonine residues by GSK3B; phosphorylation inhibits its function (By similarity).By similarity
Polyubiquitinated, probably by NEDD4.By similarity
Keywords - PTMi
Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8CHW4 |
jPOSTi | Q8CHW4 |
MaxQBi | Q8CHW4 |
PaxDbi | Q8CHW4 |
PeptideAtlasi | Q8CHW4 |
PRIDEi | Q8CHW4 |
PTM databases
iPTMneti | Q8CHW4 |
PhosphoSitePlusi | Q8CHW4 |
SwissPalmi | Q8CHW4 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000003235, Expressed in embryo and 309 other tissues |
ExpressionAtlasi | Q8CHW4, baseline and differential |
Genevisiblei | Q8CHW4, MM |
Interactioni
Subunit structurei
Complex of five different subunits; alpha, beta, gamma, delta and epsilon.
Interacts with RGS2 (By similarity).
By similarityGO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: MGI
- translation initiation factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 230231, 11 interactors |
STRINGi | 10090.ENSMUSP00000003320 |
Miscellaneous databases
RNActi | Q8CHW4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 539 – 716 | W2PROSITE-ProRule annotationAdd BLAST | 178 |
Sequence similaritiesi
Belongs to the eIF-2B gamma/epsilon subunits family.Curated
Phylogenomic databases
eggNOGi | KOG1461, Eukaryota |
GeneTreei | ENSGT00510000047568 |
HOGENOMi | CLU_012507_2_0_1 |
InParanoidi | Q8CHW4 |
OMAi | LRMSMNV |
OrthoDBi | 369837at2759 |
PhylomeDBi | Q8CHW4 |
TreeFami | TF101509 |
Family and domain databases
CDDi | cd04197, eIF-2B_epsilon_N, 1 hit |
Gene3Di | 1.25.40.180, 1 hit 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR035543, eIF-2B_epsilon_N IPR001451, Hexapep IPR016021, MIF4-like_sf IPR029044, Nucleotide-diphossugar_trans IPR011004, Trimer_LpxA-like_sf IPR003307, W2_domain |
Pfami | View protein in Pfam PF00132, Hexapep, 1 hit PF02020, W2, 1 hit |
SMARTi | View protein in SMART SM00515, eIF5C, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF51161, SSF51161, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS51363, W2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q8CHW4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAATAAVPGA AAGRASKRGG GGSGGGGTQG AEEEPPPPLQ AVLVADSFNR
60 70 80 90 100
RFFPISKDQP RVLLPLANVA LIDYTLEFLT ATGVQETFVF CCWKAAQIKE
110 120 130 140 150
HLQKSKWCHP TSPNVVRIIT SELYRSLGDV LRDVDAKALV RSDFLLIYGD
160 170 180 190 200
VISNINICRA LEEHRLRRKL EKNVSVMTMV FKESSPSHPT RCHEDNVVMA
210 220 230 240 250
VDSATNRVLH FQKTQGLRRF SFPLSLFQGS GDGVEIRYDL LDCHISICSP
260 270 280 290 300
QVAQLFTDNF DYQTRDDFVR GILMNEEVLG NQIHLHVTTR EYGARVSNLH
310 320 330 340 350
MYSAVCADVI RRWVYPLTPE VNFTDSTTQS YTHSRHNIYR GPEVSLGHGS
360 370 380 390 400
VLEENVLLGA GTVIGSNCSI TNSVIGPNCH IGDNVVLDQA YLWQGVRVAA
410 420 430 440 450
GAQIHQSLLC DRAEVKERVK LKPYCVLTSQ VVVGPDITLP EGSVISLHPP
460 470 480 490 500
DAEEDEDDGQ FSDDSGADQE KEKVKLKGYN PAEVGLEGQG YLWKAEGVNS
510 520 530 540 550
KEDEELRQSL WGLMIKTEEE SETESEGSVD PEELDSRAGS PQLDDIRVFQ
560 570 580 590 600
NEVLGTLQRG REENISCENL VLEINSLKHA YNISLKEVMQ VLTLVVLEFP
610 620 630 640 650
LQQVDGLLDP NRYCALLLPL LKAWSPVLRN YIKRAADHLE ALAAIEDFFL
660 670 680 690 700
EHETLVTSMA KVLMAFYQLE ILAEETILSW FSQRDTTDEG QQLRKNQQLQ
710
RFIQWLREAE EESSEDD
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A338P6D9 | A0A338P6D9_MOUSE | Translation initiation factor eIF-2... | Eif2b5 | 303 | Annotation score: | ||
F6T9C3 | F6T9C3_MOUSE | Translation initiation factor eIF-2... | Eif2b5 | 160 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 54 | P → H in BAE36132 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK160985 mRNA Translation: BAE36132.1 BC038620 mRNA Translation: AAH38620.1 BC085255 mRNA Translation: AAH85255.1 |
CCDSi | CCDS28047.1 |
RefSeqi | NP_758469.1, NM_172265.2 |
Genome annotation databases
Ensembli | ENSMUST00000003320; ENSMUSP00000003320; ENSMUSG00000003235 |
GeneIDi | 224045 |
KEGGi | mmu:224045 |
UCSCi | uc007ypu.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK160985 mRNA Translation: BAE36132.1 BC038620 mRNA Translation: AAH38620.1 BC085255 mRNA Translation: AAH85255.1 |
CCDSi | CCDS28047.1 |
RefSeqi | NP_758469.1, NM_172265.2 |
3D structure databases
SMRi | Q8CHW4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230231, 11 interactors |
STRINGi | 10090.ENSMUSP00000003320 |
PTM databases
iPTMneti | Q8CHW4 |
PhosphoSitePlusi | Q8CHW4 |
SwissPalmi | Q8CHW4 |
Proteomic databases
EPDi | Q8CHW4 |
jPOSTi | Q8CHW4 |
MaxQBi | Q8CHW4 |
PaxDbi | Q8CHW4 |
PeptideAtlasi | Q8CHW4 |
PRIDEi | Q8CHW4 |
Protocols and materials databases
Antibodypediai | 4211, 157 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000003320; ENSMUSP00000003320; ENSMUSG00000003235 |
GeneIDi | 224045 |
KEGGi | mmu:224045 |
UCSCi | uc007ypu.2, mouse |
Organism-specific databases
CTDi | 8893 |
MGIi | MGI:2446176, Eif2b5 |
Phylogenomic databases
eggNOGi | KOG1461, Eukaryota |
GeneTreei | ENSGT00510000047568 |
HOGENOMi | CLU_012507_2_0_1 |
InParanoidi | Q8CHW4 |
OMAi | LRMSMNV |
OrthoDBi | 369837at2759 |
PhylomeDBi | Q8CHW4 |
TreeFami | TF101509 |
Enzyme and pathway databases
Reactomei | R-MMU-72731, Recycling of eIF2:GDP |
Miscellaneous databases
BioGRID-ORCSi | 224045, 8 hits in 17 CRISPR screens |
ChiTaRSi | Eif2b5, mouse |
PROi | PR:Q8CHW4 |
RNActi | Q8CHW4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000003235, Expressed in embryo and 309 other tissues |
ExpressionAtlasi | Q8CHW4, baseline and differential |
Genevisiblei | Q8CHW4, MM |
Family and domain databases
CDDi | cd04197, eIF-2B_epsilon_N, 1 hit |
Gene3Di | 1.25.40.180, 1 hit 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR016024, ARM-type_fold IPR035543, eIF-2B_epsilon_N IPR001451, Hexapep IPR016021, MIF4-like_sf IPR029044, Nucleotide-diphossugar_trans IPR011004, Trimer_LpxA-like_sf IPR003307, W2_domain |
Pfami | View protein in Pfam PF00132, Hexapep, 1 hit PF02020, W2, 1 hit |
SMARTi | View protein in SMART SM00515, eIF5C, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF51161, SSF51161, 1 hit SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS51363, W2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EI2BE_MOUSE | |
Accessioni | Q8CHW4Primary (citable) accession number: Q8CHW4 Secondary accession number(s): Q3TU39 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 13, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families