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Protein

Epsin-2

Gene

Epn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in the formation of clathrin-coated invaginations and endocytosis.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei8Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei11Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei25Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei30Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei63Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei73Phosphatidylinositol lipid headgroupBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-2
Alternative name(s):
EPS-15-interacting protein 2
Intersectin-EH-binding protein 2
Short name:
Ibp2
Gene namesi
Name:Epn2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1333766 Epn2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745171 – 595Epsin-2Add BLAST595

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei170Omega-N-methylarginineCombined sources1
Modified residuei173PhosphoserineCombined sources1
Modified residuei192PhosphoserineCombined sources1
Modified residuei195PhosphoserineBy similarity1
Modified residuei443PhosphoserineBy similarity1
Modified residuei465PhosphothreonineBy similarity1
Modified residuei526PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated.By similarity

Keywords - PTMi

Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PeptideAtlasiQ8CHU3
PRIDEiQ8CHU3

PTM databases

iPTMnetiQ8CHU3
PhosphoSitePlusiQ8CHU3

Expressioni

Gene expression databases

BgeeiENSMUSG00000001036 Expressed in 286 organ(s), highest expression level in secondary oocyte
CleanExiMM_EPN2
ExpressionAtlasiQ8CHU3 baseline and differential
GenevisibleiQ8CHU3 MM

Interactioni

Subunit structurei

Binds EPS15, AP-2 and clathrin (By similarity). Interacts with UBQLN2 (By similarity). Interacts with ITSN1.By similarity1 Publication

Protein-protein interaction databases

BioGridi199486, 5 interactors
ELMiQ8CHU3
IntActiQ8CHU3, 3 interactors
MINTiQ8CHU3

Structurei

3D structure databases

ProteinModelPortaliQ8CHU3
SMRiQ8CHU3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 144ENTHPROSITE-ProRule annotationAdd BLAST133
Domaini218 – 237UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini255 – 274UIM 2PROSITE-ProRule annotationAdd BLAST20
Repeati313 – 31513
Repeati325 – 32723
Repeati338 – 34033
Repeati352 – 35443
Repeati370 – 37253
Repeati387 – 38963
Repeati494 – 49613
Repeati508 – 51023
Repeati591 – 59333

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni313 – 3896 X 3 AA repeats of [DE]-P-WAdd BLAST77
Regioni494 – 5933 X 3 AA repeats of N-P-FAdd BLAST100

Domaini

The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP-2 and clathrin binding.

Sequence similaritiesi

Belongs to the epsin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000074611
HOGENOMiHOG000008298
HOVERGENiHBG006690
InParanoidiQ8CHU3
KOiK12471
OMAiEQEYGKS
PhylomeDBiQ8CHU3
TreeFamiTF313361

Family and domain databases

CDDicd03571 ENTH_epsin, 1 hit
Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR013809 ENTH
IPR008942 ENTH_VHS
IPR027319 Epsin-2_metazoa
IPR039416 Epsin_ENTH
IPR003903 UIM_dom
PANTHERiPTHR12276:SF50 PTHR12276:SF50, 1 hit
PfamiView protein in Pfam
PF01417 ENTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SM00726 UIM, 2 hits
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit
PS50330 UIM, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8CHU3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTTSSIRRQM KNIVNNYSEA EIKVREATSN DPWGPSSSLM TEIADLTYNV
60 70 80 90 100
VAFSEIMSMV WKRLNDHGKN WRHVYKALTL LDYLIKTGSE RVAQQCRENI
110 120 130 140 150
FAIQTLKDFQ YIDRDGKDQG INVREKSKQL VALLKDEERL KVERVQALKT
160 170 180 190 200
KERMAQVATG VGSNQITFGR GSSQPNLSTS YSEQEYGKAG GSPASYHGST
210 220 230 240 250
SPRVSSELEQ ARPQTSGEEE LQLQLALAMS REVAEQSSES VQTARGSKEE
260 270 280 290 300
RLRRGDDLRL QMALEESRRD TVKVPKKKEA KACCKPGSHS QQTTLLDLMD
310 320 330 340 350
ALPSSGPVTQ KTEPWSAGAS ANQTNPWGGT VAPSNITDPW PSFGTKPAAS
360 370 380 390 400
VDPWGVPTTA STQSVPKNSD PWAASQQPAS NAGKTTDAWG AAKPSSASGS
410 420 430 440 450
FELFSNFNGT VKDDFSEFDN LRTSKKPAES GASVPPQDSR TTSPDLFESQ
460 470 480 490 500
SLTSASSKPS SARKTPESFL GPNAALVNLD SLVTKPAPPA QSLNPFLAPG
510 520 530 540 550
AAAPAPVNPF QVNQPQPLTL NQLRGSPVLG SSASFGSGPG VETVAPMTSV
560 570 580 590
APHSSVGASG SSLTPLGPTA MNMVGSVGIP PSAAQSTGTT NPFLL
Length:595
Mass (Da):63,472
Last modified:March 1, 2003 - v1
Checksum:i255DC7BC7E1B2D13
GO
Isoform 2 (identifier: Q8CHU3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     237-248: Missing.

Note: No experimental confirmation available.
Show »
Length:583
Mass (Da):62,253
Checksum:iEFC06A1371A85980
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3QNT7J3QNT7_MOUSE
Epsin-2
Epn2
640Annotation score:
Q5NCM5Q5NCM5_MOUSE
Epsin-2
Epn2
634Annotation score:
Q5NCM6Q5NCM6_MOUSE
Epsin-2
Epn2
577Annotation score:
F7CUV7F7CUV7_MOUSE
Epsin-2
Epn2
274Annotation score:
F7CD65F7CD65_MOUSE
Epsin-2
Epn2
148Annotation score:
A8Y5D4A8Y5D4_MOUSE
Epsin-2
Epn2
79Annotation score:
A8Y5D5A8Y5D5_MOUSE
Epsin-2
Epn2
17Annotation score:

Sequence cautioni

The sequence AAC97476 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75Y → S in AAC97476 (PubMed:9813051).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009157237 – 248Missing in isoform 2. 2 PublicationsAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036331 mRNA Translation: BAC29387.1
AL604029 Genomic DNA No translation available.
BC039138 mRNA Translation: AAH39138.1
AF057286 mRNA Translation: AAC97476.1 Different initiation.
CCDSiCCDS24816.1 [Q8CHU3-1]
CCDS56779.1 [Q8CHU3-2]
RefSeqiNP_001239118.1, NM_001252189.1 [Q8CHU3-2]
NP_034278.1, NM_010148.3 [Q8CHU3-1]
XP_017169742.1, XM_017314253.1
UniGeneiMm.139695
Mm.489800

Genome annotation databases

EnsembliENSMUST00000001063; ENSMUSP00000001063; ENSMUSG00000001036 [Q8CHU3-1]
ENSMUST00000108713; ENSMUSP00000104353; ENSMUSG00000001036 [Q8CHU3-2]
ENSMUST00000178202; ENSMUSP00000136553; ENSMUSG00000001036 [Q8CHU3-1]
GeneIDi13855
KEGGimmu:13855
UCSCiuc007jhv.2 mouse [Q8CHU3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036331 mRNA Translation: BAC29387.1
AL604029 Genomic DNA No translation available.
BC039138 mRNA Translation: AAH39138.1
AF057286 mRNA Translation: AAC97476.1 Different initiation.
CCDSiCCDS24816.1 [Q8CHU3-1]
CCDS56779.1 [Q8CHU3-2]
RefSeqiNP_001239118.1, NM_001252189.1 [Q8CHU3-2]
NP_034278.1, NM_010148.3 [Q8CHU3-1]
XP_017169742.1, XM_017314253.1
UniGeneiMm.139695
Mm.489800

3D structure databases

ProteinModelPortaliQ8CHU3
SMRiQ8CHU3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199486, 5 interactors
ELMiQ8CHU3
IntActiQ8CHU3, 3 interactors
MINTiQ8CHU3

PTM databases

iPTMnetiQ8CHU3
PhosphoSitePlusiQ8CHU3

Proteomic databases

PeptideAtlasiQ8CHU3
PRIDEiQ8CHU3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001063; ENSMUSP00000001063; ENSMUSG00000001036 [Q8CHU3-1]
ENSMUST00000108713; ENSMUSP00000104353; ENSMUSG00000001036 [Q8CHU3-2]
ENSMUST00000178202; ENSMUSP00000136553; ENSMUSG00000001036 [Q8CHU3-1]
GeneIDi13855
KEGGimmu:13855
UCSCiuc007jhv.2 mouse [Q8CHU3-1]

Organism-specific databases

CTDi22905
MGIiMGI:1333766 Epn2

Phylogenomic databases

GeneTreeiENSGT00550000074611
HOGENOMiHOG000008298
HOVERGENiHBG006690
InParanoidiQ8CHU3
KOiK12471
OMAiEQEYGKS
PhylomeDBiQ8CHU3
TreeFamiTF313361

Enzyme and pathway databases

ReactomeiR-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

ChiTaRSiEpn2 mouse
PROiPR:Q8CHU3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001036 Expressed in 286 organ(s), highest expression level in secondary oocyte
CleanExiMM_EPN2
ExpressionAtlasiQ8CHU3 baseline and differential
GenevisibleiQ8CHU3 MM

Family and domain databases

CDDicd03571 ENTH_epsin, 1 hit
Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR013809 ENTH
IPR008942 ENTH_VHS
IPR027319 Epsin-2_metazoa
IPR039416 Epsin_ENTH
IPR003903 UIM_dom
PANTHERiPTHR12276:SF50 PTHR12276:SF50, 1 hit
PfamiView protein in Pfam
PF01417 ENTH, 1 hit
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SM00726 UIM, 2 hits
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit
PS50330 UIM, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiEPN2_MOUSE
AccessioniPrimary (citable) accession number: Q8CHU3
Secondary accession number(s): O70447, Q5NCM4, Q8BZ85
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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