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UniProtKB - Q8CHU3 (EPN2_MOUSE)
Protein
Epsin-2
Gene
Epn2
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a role in the formation of clathrin-coated invaginations and endocytosis.
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 8 | Phosphatidylinositol 4,5-bisphosphateBy similarity | 1 | |
Binding sitei | 11 | Phosphatidylinositol 4,5-bisphosphateBy similarity | 1 | |
Binding sitei | 25 | Phosphatidylinositol 4,5-bisphosphateBy similarity | 1 | |
Binding sitei | 30 | Phosphatidylinositol 4,5-bisphosphateBy similarity | 1 | |
Binding sitei | 63 | Phosphatidylinositol 4,5-bisphosphateBy similarity | 1 | |
Binding sitei | 73 | Phosphatidylinositol 4,5-bisphosphateBy similarity | 1 |
GO - Molecular functioni
- clathrin binding Source: GO_Central
- phospholipid binding Source: GO_Central
GO - Biological processi
- embryonic organ development Source: MGI
- endocytosis Source: GO_Central
- in utero embryonic development Source: MGI
- negative regulation of sprouting angiogenesis Source: MGI
- negative regulation of vascular endothelial growth factor receptor signaling pathway Source: MGI
- Notch signaling pathway Source: MGI
- positive regulation of Notch signaling pathway Source: MGI
- regulation of endocytosis Source: MGI
Keywordsi
Biological process | Endocytosis |
Ligand | Lipid-binding |
Enzyme and pathway databases
Reactomei | R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: Epsin-2Alternative name(s): EPS-15-interacting protein 2 Intersectin-EH-binding protein 2 Short name: Ibp2 |
Gene namesi | Name:Epn2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1333766, Epn2 |
VEuPathDBi | HostDB:ENSMUSG00000001036 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Note: In punctate structures throughout the cell and particularly concentrated in the region of the Golgi complex.By similarity
Endosome
- endosome Source: GO_Central
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- clathrin coat of endocytic vesicle Source: MGI
- clathrin vesicle coat Source: GO_Central
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074517 | 1 – 595 | Epsin-2Add BLAST | 595 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 170 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 173 | PhosphoserineCombined sources | 1 | |
Modified residuei | 192 | PhosphoserineCombined sources | 1 | |
Modified residuei | 195 | PhosphoserineBy similarity | 1 | |
Modified residuei | 443 | PhosphoserineBy similarity | 1 | |
Modified residuei | 465 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 526 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Ubiquitinated.By similarity
Keywords - PTMi
Methylation, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q8CHU3 |
MaxQBi | Q8CHU3 |
PeptideAtlasi | Q8CHU3 |
PRIDEi | Q8CHU3 |
ProteomicsDBi | 275634 [Q8CHU3-1] 275635 [Q8CHU3-2] |
PTM databases
iPTMneti | Q8CHU3 |
PhosphoSitePlusi | Q8CHU3 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000001036, Expressed in secondary oocyte and 305 other tissues |
ExpressionAtlasi | Q8CHU3, baseline and differential |
Genevisiblei | Q8CHU3, MM |
Interactioni
Subunit structurei
Binds EPS15, AP-2 and clathrin (By similarity).
Interacts with UBQLN2 (By similarity).
Interacts with ITSN1.
By similarity1 PublicationGO - Molecular functioni
- clathrin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 199486, 8 interactors |
ELMi | Q8CHU3 |
IntActi | Q8CHU3, 1 interactor |
MINTi | Q8CHU3 |
STRINGi | 10090.ENSMUSP00000001063 |
Miscellaneous databases
RNActi | Q8CHU3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 144 | ENTHPROSITE-ProRule annotationAdd BLAST | 133 | |
Domaini | 218 – 237 | UIM 1PROSITE-ProRule annotationAdd BLAST | 20 | |
Domaini | 255 – 274 | UIM 2PROSITE-ProRule annotationAdd BLAST | 20 | |
Repeati | 313 – 315 | 1 | 3 | |
Repeati | 325 – 327 | 2 | 3 | |
Repeati | 338 – 340 | 3 | 3 | |
Repeati | 352 – 354 | 4 | 3 | |
Repeati | 370 – 372 | 5 | 3 | |
Repeati | 387 – 389 | 6 | 3 | |
Repeati | 494 – 496 | 1 | 3 | |
Repeati | 508 – 510 | 2 | 3 | |
Repeati | 591 – 593 | 3 | 3 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 164 – 254 | DisorderedSequence analysisAdd BLAST | 91 | |
Regioni | 267 – 289 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 305 – 396 | DisorderedSequence analysisAdd BLAST | 92 | |
Regioni | 313 – 389 | 6 X 3 AA repeats of [DE]-P-WAdd BLAST | 77 | |
Regioni | 423 – 469 | DisorderedSequence analysisAdd BLAST | 47 | |
Regioni | 494 – 593 | 3 X 3 AA repeats of N-P-FAdd BLAST | 100 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 164 – 186 | Polar residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 194 – 221 | Polar residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 267 – 284 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 305 – 341 | Polar residuesSequence analysisAdd BLAST | 37 | |
Compositional biasi | 355 – 384 | Polar residuesSequence analysisAdd BLAST | 30 | |
Compositional biasi | 434 – 467 | Polar residuesSequence analysisAdd BLAST | 34 |
Domaini
The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP-2 and clathrin binding.
Sequence similaritiesi
Belongs to the epsin family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2056, Eukaryota |
GeneTreei | ENSGT00940000157239 |
HOGENOMi | CLU_012678_4_2_1 |
InParanoidi | Q8CHU3 |
OMAi | EKQSHML |
OrthoDBi | 1263849at2759 |
PhylomeDBi | Q8CHU3 |
TreeFami | TF313361 |
Family and domain databases
Gene3Di | 1.25.40.90, 1 hit |
InterProi | View protein in InterPro IPR013809, ENTH IPR008942, ENTH_VHS IPR027319, Epsin-2_metazoa IPR003903, UIM_dom |
PANTHERi | PTHR12276:SF50, PTHR12276:SF50, 1 hit |
Pfami | View protein in Pfam PF01417, ENTH, 1 hit |
SMARTi | View protein in SMART SM00273, ENTH, 1 hit SM00726, UIM, 2 hits |
SUPFAMi | SSF48464, SSF48464, 1 hit |
PROSITEi | View protein in PROSITE PS50942, ENTH, 1 hit PS50330, UIM, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8CHU3-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTTSSIRRQM KNIVNNYSEA EIKVREATSN DPWGPSSSLM TEIADLTYNV
60 70 80 90 100
VAFSEIMSMV WKRLNDHGKN WRHVYKALTL LDYLIKTGSE RVAQQCRENI
110 120 130 140 150
FAIQTLKDFQ YIDRDGKDQG INVREKSKQL VALLKDEERL KVERVQALKT
160 170 180 190 200
KERMAQVATG VGSNQITFGR GSSQPNLSTS YSEQEYGKAG GSPASYHGST
210 220 230 240 250
SPRVSSELEQ ARPQTSGEEE LQLQLALAMS REVAEQSSES VQTARGSKEE
260 270 280 290 300
RLRRGDDLRL QMALEESRRD TVKVPKKKEA KACCKPGSHS QQTTLLDLMD
310 320 330 340 350
ALPSSGPVTQ KTEPWSAGAS ANQTNPWGGT VAPSNITDPW PSFGTKPAAS
360 370 380 390 400
VDPWGVPTTA STQSVPKNSD PWAASQQPAS NAGKTTDAWG AAKPSSASGS
410 420 430 440 450
FELFSNFNGT VKDDFSEFDN LRTSKKPAES GASVPPQDSR TTSPDLFESQ
460 470 480 490 500
SLTSASSKPS SARKTPESFL GPNAALVNLD SLVTKPAPPA QSLNPFLAPG
510 520 530 540 550
AAAPAPVNPF QVNQPQPLTL NQLRGSPVLG SSASFGSGPG VETVAPMTSV
560 570 580 590
APHSSVGASG SSLTPLGPTA MNMVGSVGIP PSAAQSTGTT NPFLL
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketJ3QNT7 | J3QNT7_MOUSE | Epsin-2 | Epn2 | 640 | Annotation score: | ||
Q5NCM6 | Q5NCM6_MOUSE | Epsin-2 | Epn2 | 577 | Annotation score: | ||
Q5NCM5 | Q5NCM5_MOUSE | Epsin-2 | Epn2 | 634 | Annotation score: | ||
F7CD65 | F7CD65_MOUSE | Epsin-2 | Epn2 | 148 | Annotation score: | ||
F7CUV7 | F7CUV7_MOUSE | Epsin-2 | Epn2 | 274 | Annotation score: | ||
A8Y5D4 | A8Y5D4_MOUSE | Epsin-2 | Epn2 | 79 | Annotation score: | ||
A8Y5D5 | A8Y5D5_MOUSE | Epsin-2 | Epn2 | 17 | Annotation score: |
Sequence cautioni
The sequence AAC97476 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 75 | Y → S in AAC97476 (PubMed:9813051).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009157 | 237 – 248 | Missing in isoform 2. 2 PublicationsAdd BLAST | 12 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK036331 mRNA Translation: BAC29387.1 AL604029 Genomic DNA No translation available. BC039138 mRNA Translation: AAH39138.1 AF057286 mRNA Translation: AAC97476.1 Different initiation. |
CCDSi | CCDS24816.1 [Q8CHU3-1] CCDS56779.1 [Q8CHU3-2] |
RefSeqi | NP_001239118.1, NM_001252189.1 [Q8CHU3-2] NP_034278.1, NM_010148.3 [Q8CHU3-1] XP_017169742.1, XM_017314253.1 |
Genome annotation databases
Ensembli | ENSMUST00000001063; ENSMUSP00000001063; ENSMUSG00000001036 [Q8CHU3-1] ENSMUST00000108713; ENSMUSP00000104353; ENSMUSG00000001036 [Q8CHU3-2] ENSMUST00000178202; ENSMUSP00000136553; ENSMUSG00000001036 [Q8CHU3-1] |
GeneIDi | 13855 |
KEGGi | mmu:13855 |
UCSCi | uc007jhv.2, mouse [Q8CHU3-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK036331 mRNA Translation: BAC29387.1 AL604029 Genomic DNA No translation available. BC039138 mRNA Translation: AAH39138.1 AF057286 mRNA Translation: AAC97476.1 Different initiation. |
CCDSi | CCDS24816.1 [Q8CHU3-1] CCDS56779.1 [Q8CHU3-2] |
RefSeqi | NP_001239118.1, NM_001252189.1 [Q8CHU3-2] NP_034278.1, NM_010148.3 [Q8CHU3-1] XP_017169742.1, XM_017314253.1 |
3D structure databases
SMRi | Q8CHU3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199486, 8 interactors |
ELMi | Q8CHU3 |
IntActi | Q8CHU3, 1 interactor |
MINTi | Q8CHU3 |
STRINGi | 10090.ENSMUSP00000001063 |
PTM databases
iPTMneti | Q8CHU3 |
PhosphoSitePlusi | Q8CHU3 |
Proteomic databases
jPOSTi | Q8CHU3 |
MaxQBi | Q8CHU3 |
PeptideAtlasi | Q8CHU3 |
PRIDEi | Q8CHU3 |
ProteomicsDBi | 275634 [Q8CHU3-1] 275635 [Q8CHU3-2] |
Protocols and materials databases
Antibodypediai | 25881, 329 antibodies from 27 providers |
DNASUi | 13855 |
Genome annotation databases
Ensembli | ENSMUST00000001063; ENSMUSP00000001063; ENSMUSG00000001036 [Q8CHU3-1] ENSMUST00000108713; ENSMUSP00000104353; ENSMUSG00000001036 [Q8CHU3-2] ENSMUST00000178202; ENSMUSP00000136553; ENSMUSG00000001036 [Q8CHU3-1] |
GeneIDi | 13855 |
KEGGi | mmu:13855 |
UCSCi | uc007jhv.2, mouse [Q8CHU3-1] |
Organism-specific databases
CTDi | 22905 |
MGIi | MGI:1333766, Epn2 |
VEuPathDBi | HostDB:ENSMUSG00000001036 |
Phylogenomic databases
eggNOGi | KOG2056, Eukaryota |
GeneTreei | ENSGT00940000157239 |
HOGENOMi | CLU_012678_4_2_1 |
InParanoidi | Q8CHU3 |
OMAi | EKQSHML |
OrthoDBi | 1263849at2759 |
PhylomeDBi | Q8CHU3 |
TreeFami | TF313361 |
Enzyme and pathway databases
Reactomei | R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
BioGRID-ORCSi | 13855, 1 hit in 64 CRISPR screens |
ChiTaRSi | Epn2, mouse |
PROi | PR:Q8CHU3 |
RNActi | Q8CHU3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000001036, Expressed in secondary oocyte and 305 other tissues |
ExpressionAtlasi | Q8CHU3, baseline and differential |
Genevisiblei | Q8CHU3, MM |
Family and domain databases
Gene3Di | 1.25.40.90, 1 hit |
InterProi | View protein in InterPro IPR013809, ENTH IPR008942, ENTH_VHS IPR027319, Epsin-2_metazoa IPR003903, UIM_dom |
PANTHERi | PTHR12276:SF50, PTHR12276:SF50, 1 hit |
Pfami | View protein in Pfam PF01417, ENTH, 1 hit |
SMARTi | View protein in SMART SM00273, ENTH, 1 hit SM00726, UIM, 2 hits |
SUPFAMi | SSF48464, SSF48464, 1 hit |
PROSITEi | View protein in PROSITE PS50942, ENTH, 1 hit PS50330, UIM, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | EPN2_MOUSE | |
Accessioni | Q8CHU3Primary (citable) accession number: Q8CHU3 Secondary accession number(s): O70447, Q5NCM4, Q8BZ85 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 16, 2004 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families