UniProtKB - Q8CHT1 (NGEF_MOUSE)
Ephexin-1
Ngef
Functioni
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.
3 PublicationsGO - Molecular functioni
- ephrin receptor binding Source: UniProtKB
- guanyl-nucleotide exchange factor activity Source: MGI
GO - Biological processi
- activation of GTPase activity Source: GO_Central
- cell differentiation Source: UniProtKB-KW
- ephrin receptor signaling pathway Source: UniProtKB
- negative regulation of dendritic spine morphogenesis Source: UniProtKB
- nervous system development Source: UniProtKB-KW
- regulation of GTPase activity Source: UniProtKB
- synapse pruning Source: SynGO
Keywordsi
Molecular function | Developmental protein, Guanine-nucleotide releasing factor |
Biological process | Differentiation, Neurogenesis |
Enzyme and pathway databases
Reactomei | R-MMU-193648, NRAGE signals death through JNK R-MMU-3928663, EPHA-mediated growth cone collapse R-MMU-416482, G alpha (12/13) signalling events R-MMU-8980692, RHOA GTPase cycle R-MMU-9013148, CDC42 GTPase cycle R-MMU-9013149, RAC1 GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Ephexin-1Alternative name(s): Eph-interacting exchange protein Neuronal guanine nucleotide exchange factor |
Gene namesi | Name:Ngef |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1858414, Ngef |
VEuPathDBi | HostDB:ENSMUSG00000026259 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- Membrane By similarity
- growth cone By similarity
Note: Associated with membranes. Localizes to axonal growth cones (By similarity).By similarity
Cytosol
- cytosol Source: Reactome
Other locations
- glutamatergic synapse Source: SynGO
- growth cone Source: UniProtKB-SubCell
- membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell projection, Cytoplasm, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 177 | Y → F: Loss of ephrin-mediated growth cone collapse. No effect on interaction with EPHA4. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000248389 | 1 – 710 | Ephexin-1Add BLAST | 710 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 177 | PhosphotyrosineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q8CHT1 |
PaxDbi | Q8CHT1 |
PRIDEi | Q8CHT1 |
ProteomicsDBi | 252961 [Q8CHT1-1] 252962 [Q8CHT1-2] |
PTM databases
iPTMneti | Q8CHT1 |
PhosphoSitePlusi | Q8CHT1 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000026259, Expressed in striatum and 206 other tissues |
Genevisiblei | Q8CHT1, MM |
Interactioni
Subunit structurei
Interacts with CDK5R1 and EPHA4; activated by EPHA4 through the CDK5 kinase.
2 PublicationsGO - Molecular functioni
- ephrin receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 207543, 12 interactors |
IntActi | Q8CHT1, 6 interactors |
MINTi | Q8CHT1 |
STRINGi | 10090.ENSMUSP00000066894 |
Miscellaneous databases
RNActi | Q8CHT1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 273 – 457 | DHPROSITE-ProRule annotationAdd BLAST | 185 | |
Domaini | 489 – 601 | PHPROSITE-ProRule annotationAdd BLAST | 113 | |
Domaini | 612 – 673 | SH3PROSITE-ProRule annotationAdd BLAST | 62 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 272 | Regulatory region; modulates activity toward RHOA, RAC1 and CDC42Add BLAST | 272 | |
Regioni | 1 – 146 | DisorderedSequence analysisAdd BLAST | 146 | |
Regioni | 192 – 234 | DisorderedSequence analysisAdd BLAST | 43 | |
Regioni | 688 – 710 | DisorderedSequence analysisAdd BLAST | 23 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 24 | Basic and acidic residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 40 – 58 | Basic and acidic residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 116 – 146 | Polar residuesSequence analysisAdd BLAST | 31 | |
Compositional biasi | 209 – 226 | Acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 688 – 703 | Basic and acidic residuesSequence analysisAdd BLAST | 16 |
Domaini
Keywords - Domaini
SH3 domainPhylogenomic databases
eggNOGi | KOG3523, Eukaryota |
GeneTreei | ENSGT01030000234571 |
HOGENOMi | CLU_012820_5_1_1 |
InParanoidi | Q8CHT1 |
OrthoDBi | 1176939at2759 |
PhylomeDBi | Q8CHT1 |
Family and domain databases
CDDi | cd00160, RhoGEF, 1 hit cd11939, SH3_ephexin1, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR035899, DBL_dom_sf IPR000219, DH-domain IPR035635, Ephexin-1_SH3 IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF00621, RhoGEF, 1 hit PF00018, SH3_1, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit PS50002, SH3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
METKNSEDWG KPQRKSESSS RKSNHGPAEM RPALPPENRE APETGEETQN
60 70 80 90 100
EEPRRLIPIQ RHSLFNRAVR HRHKARSTSE RRASDQADLP KMGKSVNERS
110 120 130 140 150
AFNLPQGRLS PWRTPAQRDT GAQEASESSS TPGNGTTPEE CPALTDSPTT
160 170 180 190 200
LTEALQMIHP IPADSWRNLI EQIGLLYQEY RDKSTLQEIE TRRQQDAEIQ
210 220 230 240 250
GNSDGSQVGE DAGEEEEEEE EGEEEELASP PERRALPQIC LLSNPHSRFN
260 270 280 290 300
LWQDLPEIQS SGVLDILQPE EIRLQEAMFE LVTSEASYYK SLNLLVSHFM
310 320 330 340 350
ENERLKKILH PSEAHILFSN VLDVMAVSER FLLELEHRME ENIVISDVCD
360 370 380 390 400
IVYRYAADHF SVYITYVSNQ TYQERTYKQL LQEKAAFREL IAQLELDPKC
410 420 430 440 450
KGLPFSSFLI LPFQRITRLK LLVQNILKRV EERSEREGTA LDAHKELEMV
460 470 480 490 500
VKACNEGVRK MSRTEQMISI QKKMEFKIKS VPIISHSRWL LKQGELQQMS
510 520 530 540 550
GPKTSRTLRT KKLFREIYLF LFNDLLVICR QIPGDKYQVF DSAPRGLLRV
560 570 580 590 600
EELEDQGQTL ANVFILRLLE NADDREATYM LKASSQSEMK RWMTSLAPNR
610 620 630 640 650
RTKFVSFTSR LLDCPQVQCV HPYVAQQPDE LTLELADILN ILEKTEDGWI
660 670 680 690 700
FGERLHDQER GWFPSSMTEE ILNPKIRSQN LKECFRVHKM EDPQRSQNKD
710
RRKLGSRNRQ
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9QK62 | E9QK62_MOUSE | Ephexin-1 | Ngef | 710 | Annotation score: | ||
F6SWQ9 | F6SWQ9_MOUSE | Ephexin-1 | Ngef | 245 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 530 | R → W in AAK71494 (PubMed:11336673).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020262 | 1 – 90 | Missing in isoform 2. 2 PublicationsAdd BLAST | 90 | |
Alternative sequenceiVSP_020263 | 91 – 125 | KMGKS…GAQEA → MELLAAAFSAACAVDHDSST SESDTRDSAAGHLPG in isoform 2. 2 PublicationsAdd BLAST | 35 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ238898 mRNA Translation: CAC00698.1 Frameshift. AY038025 mRNA Translation: AAK71494.1 BC022680 mRNA Translation: AAH22680.1 Different initiation. BC039279 mRNA Translation: AAH39279.1 |
CCDSi | CCDS48306.1 [Q8CHT1-2] CCDS48307.1 [Q8CHT1-1] |
RefSeqi | NP_001104784.1, NM_001111314.1 NP_063920.2, NM_019867.2 [Q8CHT1-2] |
Genome annotation databases
Ensembli | ENSMUST00000027477; ENSMUSP00000027477; ENSMUSG00000026259 [Q8CHT1-2] |
GeneIDi | 53972 |
KEGGi | mmu:53972 |
UCSCi | uc007bwx.2, mouse [Q8CHT1-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ238898 mRNA Translation: CAC00698.1 Frameshift. AY038025 mRNA Translation: AAK71494.1 BC022680 mRNA Translation: AAH22680.1 Different initiation. BC039279 mRNA Translation: AAH39279.1 |
CCDSi | CCDS48306.1 [Q8CHT1-2] CCDS48307.1 [Q8CHT1-1] |
RefSeqi | NP_001104784.1, NM_001111314.1 NP_063920.2, NM_019867.2 [Q8CHT1-2] |
3D structure databases
AlphaFoldDBi | Q8CHT1 |
SMRi | Q8CHT1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 207543, 12 interactors |
IntActi | Q8CHT1, 6 interactors |
MINTi | Q8CHT1 |
STRINGi | 10090.ENSMUSP00000066894 |
PTM databases
iPTMneti | Q8CHT1 |
PhosphoSitePlusi | Q8CHT1 |
Proteomic databases
MaxQBi | Q8CHT1 |
PaxDbi | Q8CHT1 |
PRIDEi | Q8CHT1 |
ProteomicsDBi | 252961 [Q8CHT1-1] 252962 [Q8CHT1-2] |
Protocols and materials databases
Antibodypediai | 2119, 140 antibodies from 21 providers |
Genome annotation databases
Ensembli | ENSMUST00000027477; ENSMUSP00000027477; ENSMUSG00000026259 [Q8CHT1-2] |
GeneIDi | 53972 |
KEGGi | mmu:53972 |
UCSCi | uc007bwx.2, mouse [Q8CHT1-2] |
Organism-specific databases
CTDi | 25791 |
MGIi | MGI:1858414, Ngef |
VEuPathDBi | HostDB:ENSMUSG00000026259 |
Phylogenomic databases
eggNOGi | KOG3523, Eukaryota |
GeneTreei | ENSGT01030000234571 |
HOGENOMi | CLU_012820_5_1_1 |
InParanoidi | Q8CHT1 |
OrthoDBi | 1176939at2759 |
PhylomeDBi | Q8CHT1 |
Enzyme and pathway databases
Reactomei | R-MMU-193648, NRAGE signals death through JNK R-MMU-3928663, EPHA-mediated growth cone collapse R-MMU-416482, G alpha (12/13) signalling events R-MMU-8980692, RHOA GTPase cycle R-MMU-9013148, CDC42 GTPase cycle R-MMU-9013149, RAC1 GTPase cycle |
Miscellaneous databases
BioGRID-ORCSi | 53972, 1 hit in 74 CRISPR screens |
ChiTaRSi | Ngef, mouse |
PROi | PR:Q8CHT1 |
RNActi | Q8CHT1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026259, Expressed in striatum and 206 other tissues |
Genevisiblei | Q8CHT1, MM |
Family and domain databases
CDDi | cd00160, RhoGEF, 1 hit cd11939, SH3_ephexin1, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR035899, DBL_dom_sf IPR000219, DH-domain IPR035635, Ephexin-1_SH3 IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF00621, RhoGEF, 1 hit PF00018, SH3_1, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit PS50002, SH3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NGEF_MOUSE | |
Accessioni | Q8CHT1Primary (citable) accession number: Q8CHT1 Secondary accession number(s): Q8R204, Q923H2, Q9JHT9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot