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Protein

Dihydropyrimidine dehydrogenase [NADP(+)]

Gene

Dpyd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine (By similarity).By similarity

Catalytic activityi

5,6-dihydrouracil + NADP+ = uracil + NADPH.

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 4 [4Fe-4S] clusters. Contains approximately 16 iron atoms per subunit.By similarity
  • FADBy similarity
  • FMNBy similarity

Activity regulationi

Inactivated by 5-iodouracil.By similarity

Pathwayi: beta-alanine biosynthesis

This protein is involved in the pathway beta-alanine biosynthesis, which is part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway beta-alanine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi79Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi82Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi87Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi91Iron-sulfur 2 (4Fe-4S)By similarity1
Binding sitei129FAD; via carbonyl oxygenBy similarity1
Metal bindingi130Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi136Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi140Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi156Iron-sulfur 2 (4Fe-4S)By similarity1
Binding sitei235FADBy similarity1
Binding sitei261FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei371NADPBy similarity1
Binding sitei550FMNBy similarity1
Binding sitei609SubstrateBy similarity1
Active sitei671Proton acceptorBy similarity1
Binding sitei709FMNBy similarity1
Binding sitei767FMN; via amide nitrogenBy similarity1
Metal bindingi953Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi956Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi959Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi963Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi986Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi989Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi992Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi996Iron-sulfur 4 (4Fe-4S)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi194 – 198FADBy similarity5
Nucleotide bindingi218 – 226FADBy similarity9
Nucleotide bindingi340 – 343NADPBy similarity4
Nucleotide bindingi364 – 365NADPBy similarity2
Nucleotide bindingi437 – 439NADPBy similarity3
Nucleotide bindingi480 – 489FADBy similarity10
Nucleotide bindingi481 – 487NADPBy similarity7
Nucleotide bindingi574 – 575FMNBy similarity2
Nucleotide bindingi793 – 795FMNBy similarity3
Nucleotide bindingi816 – 817FMNBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Ligand4Fe-4S, FAD, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-73621 Pyrimidine catabolism
UniPathwayi
UPA00131

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydropyrimidine dehydrogenase [NADP(+)] (EC:1.3.1.2)
Short name:
DHPDHase
Short name:
DPD
Alternative name(s):
Dihydrothymine dehydrogenase
Dihydrouracil dehydrogenase
Gene namesi
Name:Dpyd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2139667 Dpyd

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003275011 – 1025Dihydropyrimidine dehydrogenase [NADP(+)]Add BLAST1025

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei384N6-acetyllysineBy similarity1
Modified residuei905PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8CHR6
PaxDbiQ8CHR6
PeptideAtlasiQ8CHR6
PRIDEiQ8CHR6

PTM databases

iPTMnetiQ8CHR6
PhosphoSitePlusiQ8CHR6
SwissPalmiQ8CHR6

Expressioni

Gene expression databases

BgeeiENSMUSG00000033308 Expressed in 250 organ(s), highest expression level in liver
ExpressionAtlasiQ8CHR6 baseline and differential
GenevisibleiQ8CHR6 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8CHR6, 2 interactors
MINTiQ8CHR6
STRINGi10090.ENSMUSP00000039429

Structurei

3D structure databases

ProteinModelPortaliQ8CHR6
SMRiQ8CHR6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 1004Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST32
Domaini944 – 9764Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST33
Domaini978 – 10074Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni668 – 670Substrate bindingBy similarity3
Regioni736 – 737Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0399 Eukaryota
COG0167 LUCA
COG0493 LUCA
COG1146 LUCA
GeneTreeiENSGT00500000044896
HOGENOMiHOG000007797
HOVERGENiHBG004351
InParanoidiQ8CHR6
KOiK00207
OMAiEQGWGFV
OrthoDBiEOG091G00YL
PhylomeDBiQ8CHR6
TreeFamiTF105791

Family and domain databases

Gene3Di1.10.1060.10, 1 hit
3.20.20.70, 1 hit
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR013785 Aldolase_TIM
IPR005720 Dihydroorotate_DH
IPR028261 DPD_II
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR009051 Helical_ferredxn
PfamiView protein in Pfam
PF01180 DHO_dh, 1 hit
PF14691 Fer4_20, 1 hit
PF07992 Pyr_redox_2, 1 hit
SUPFAMiSSF46548 SSF46548, 1 hit
TIGRFAMsiTIGR01037 pyrD_sub1_fam, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 1 hit
PS51379 4FE4S_FER_2, 3 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8CHR6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGVLSRDAP DIESILALNP RVQAHATLRS TAAKKLDKKH WKRNTDKNCF
60 70 80 90 100
TCEKLESNFD DIKHTTLGER GALREAVRCL KCADAPCQKS CPTSLDIKSF
110 120 130 140 150
ITSIANKNYY GAAKLIFSDN PLGLTCGMVC PTSDLCVGGC NLHAAEEGPI
160 170 180 190 200
NIGGLQQFAT EVFKAMNIPQ IRNPSLPPPE HMPEAYSAKI ALFGAGPASI
210 220 230 240 250
SCASFLARLG YSNITIFEKQ EYVGGLSTSE IPQFRLPYDV VNFEIELMKD
260 270 280 290 300
LGVKIICGKS LSTDEMTLSS LKENGYRAAF IGIGLPEPKK DHIFQGLTQV
310 320 330 340 350
QGFYTSKDFL PLVAKSSKTG MCACHSPLPS IRGAVIVLGA GDTAFDCATS
360 370 380 390 400
ALRCGALRVF IVFRKGFVNI RAVPEEMELA KEEKCEFLPF LSPRKVIVKD
410 420 430 440 450
GKIVAMQFVR TEQDETGNWV EDEEQTVRLK ADVVISAFGS VLEDPKVKEA
460 470 480 490 500
LSPIKFNRWG LPEVNPETMQ TSEPWVFAGG DVVGMANTTV ESVNDGKQAS
510 520 530 540 550
WYIHKHIQAQ YGTSVPSQPT MPLFYTPVDL VDISVEMAGL RFPNPFGLAS
560 570 580 590 600
ATPATSTPMI RRAFEAGWGF ALTKTFSLDK DIVTNVSPRI IRGTTSGPLY
610 620 630 640 650
GPGQSSFLNI ELISEKTAAY WCHSVTELKA DFPDNILIAS IMCSYNKSDW
660 670 680 690 700
MELSKMAEAS GADALELNLS CPHGMGERGM GLACGQDPEL VRNICRWVRQ
710 720 730 740 750
AVRVPFFAKL TPNVTDIVSI ARAAKEGGAD GVTATNTVSG LMGLKADGTP
760 770 780 790 800
WPAVGIGRRT TYGGVSGTAI RPIALRAVTA IARALPGFPI LATGGIDSAE
810 820 830 840 850
SGLQFLHSGA SVLQVCSAIQ NQDFTVIEDY CTGLKALLYL KSIEELADWD
860 870 880 890 900
GQSPPIISHQ KGKPVPRVAE LMGQKLPSFG PYLEQRKKII AASKIRQKDQ
910 920 930 940 950
NTACSPLQRK HFNSQKPIPA IKDVIGKSLQ YLGTFGEMSI MEQVVALIDE
960 970 980 990 1000
EMCINCGKCY MTCNDSGYQA IQFDPETHLP TVSDTCTGCT LCLSVCPIMD
1010 1020
CIRMVSRATP YQPKRGLPLA VKPVC
Length:1,025
Mass (Da):111,253
Last modified:March 1, 2003 - v1
Checksum:iAA1D1B3E3B72C504
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JF48A0A0G2JF48_MOUSE
Dihydropyrimidine dehydrogenase [NA...
Dpyd
78Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC039699 mRNA Translation: AAH39699.1
CCDSiCCDS17797.1
RefSeqiNP_740748.1, NM_170778.2
UniGeneiMm.27907

Genome annotation databases

EnsembliENSMUST00000039177; ENSMUSP00000039429; ENSMUSG00000033308
GeneIDi99586
KEGGimmu:99586
UCSCiuc008rdh.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC039699 mRNA Translation: AAH39699.1
CCDSiCCDS17797.1
RefSeqiNP_740748.1, NM_170778.2
UniGeneiMm.27907

3D structure databases

ProteinModelPortaliQ8CHR6
SMRiQ8CHR6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CHR6, 2 interactors
MINTiQ8CHR6
STRINGi10090.ENSMUSP00000039429

PTM databases

iPTMnetiQ8CHR6
PhosphoSitePlusiQ8CHR6
SwissPalmiQ8CHR6

Proteomic databases

MaxQBiQ8CHR6
PaxDbiQ8CHR6
PeptideAtlasiQ8CHR6
PRIDEiQ8CHR6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039177; ENSMUSP00000039429; ENSMUSG00000033308
GeneIDi99586
KEGGimmu:99586
UCSCiuc008rdh.1 mouse

Organism-specific databases

CTDi1806
MGIiMGI:2139667 Dpyd

Phylogenomic databases

eggNOGiKOG0399 Eukaryota
COG0167 LUCA
COG0493 LUCA
COG1146 LUCA
GeneTreeiENSGT00500000044896
HOGENOMiHOG000007797
HOVERGENiHBG004351
InParanoidiQ8CHR6
KOiK00207
OMAiEQGWGFV
OrthoDBiEOG091G00YL
PhylomeDBiQ8CHR6
TreeFamiTF105791

Enzyme and pathway databases

UniPathwayi
UPA00131

ReactomeiR-MMU-73621 Pyrimidine catabolism

Miscellaneous databases

PROiPR:Q8CHR6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033308 Expressed in 250 organ(s), highest expression level in liver
ExpressionAtlasiQ8CHR6 baseline and differential
GenevisibleiQ8CHR6 MM

Family and domain databases

Gene3Di1.10.1060.10, 1 hit
3.20.20.70, 1 hit
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR013785 Aldolase_TIM
IPR005720 Dihydroorotate_DH
IPR028261 DPD_II
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR009051 Helical_ferredxn
PfamiView protein in Pfam
PF01180 DHO_dh, 1 hit
PF14691 Fer4_20, 1 hit
PF07992 Pyr_redox_2, 1 hit
SUPFAMiSSF46548 SSF46548, 1 hit
TIGRFAMsiTIGR01037 pyrD_sub1_fam, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 1 hit
PS51379 4FE4S_FER_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiDPYD_MOUSE
AccessioniPrimary (citable) accession number: Q8CHR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 1, 2003
Last modified: September 12, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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