UniProtKB - Q8CHP6 (PHC3_MOUSE)
Protein
Polyhomeotic-like protein 3
Gene
Phc3
Organism
Mus musculus (Mouse)
Status
Functioni
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 774 – 808 | FCS-typePROSITE-ProRule annotationAdd BLAST | 35 |
GO - Molecular functioni
- chromatin binding Source: GO_Central
- DNA binding Source: UniProtKB-KW
- histone binding Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- multicellular organism development Source: UniProtKB-KW
- negative regulation of transcription, DNA-templated Source: GO_Central
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-3108214, SUMOylation of DNA damage response and repair proteins R-MMU-3899300, SUMOylation of transcription cofactors R-MMU-4551638, SUMOylation of chromatin organization proteins R-MMU-4570464, SUMOylation of RNA binding proteins R-MMU-8939243, RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known R-MMU-8943724, Regulation of PTEN gene transcription R-MMU-8953750, Transcriptional Regulation by E2F6 |
Names & Taxonomyi
Protein namesi | Recommended name: Polyhomeotic-like protein 3 |
Gene namesi | Name:Phc3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2181434, Phc3 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
- PcG protein complex Source: MGI
- PRC1 complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076291 | 1 – 981 | Polyhomeotic-like protein 3Add BLAST | 981 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 231 | PhosphoserineCombined sources | 1 | |
Modified residuei | 261 | PhosphoserineBy similarity | 1 | |
Modified residuei | 269 | PhosphoserineBy similarity | 1 | |
Modified residuei | 312 | PhosphoserineBy similarity | 1 | |
Modified residuei | 607 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 612 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 614 | PhosphoserineCombined sources | 1 | |
Cross-linki | 689 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 730 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 759 | PhosphoserineBy similarity | 1 | |
Modified residuei | 760 | PhosphoserineCombined sources | 1 | |
Cross-linki | 808 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8CHP6 |
jPOSTi | Q8CHP6 |
PaxDbi | Q8CHP6 |
PRIDEi | Q8CHP6 |
PTM databases
iPTMneti | Q8CHP6 |
PhosphoSitePlusi | Q8CHP6 |
Expressioni
Tissue specificityi
Ubiquitous expression.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000037652, Expressed in mesenteric lymph node and 257 other tissues |
Genevisiblei | Q8CHP6, MM |
Interactioni
Subunit structurei
Component of a PRC1-like complex.
By similarityGO - Molecular functioni
- histone binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 232356, 6 interactors |
IntActi | Q8CHP6, 3 interactors |
MINTi | Q8CHP6 |
STRINGi | 10090.ENSMUSP00000130142 |
Miscellaneous databases
RNActi | Q8CHP6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 917 – 981 | SAMPROSITE-ProRule annotationAdd BLAST | 65 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 689 – 718 | HD1Add BLAST | 30 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 18 – 25 | Poly-Thr | 8 | |
Compositional biasi | 31 – 99 | Gln-richAdd BLAST | 69 | |
Compositional biasi | 321 – 537 | Gln-richAdd BLAST | 217 | |
Compositional biasi | 339 – 615 | Pro-richAdd BLAST | 277 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 774 – 808 | FCS-typePROSITE-ProRule annotationAdd BLAST | 35 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502QS5Q, Eukaryota |
GeneTreei | ENSGT00940000154964 |
InParanoidi | Q8CHP6 |
OMAi | ANCNQAQ |
OrthoDBi | 298184at2759 |
PhylomeDBi | Q8CHP6 |
TreeFami | TF331299 |
Family and domain databases
Gene3Di | 1.10.150.50, 1 hit 3.30.60.160, 1 hit |
InterProi | View protein in InterPro IPR001660, SAM IPR013761, SAM/pointed_sf IPR012313, Znf_FCS IPR038603, Znf_FCS_sf |
Pfami | View protein in Pfam PF00536, SAM_1, 1 hit |
SMARTi | View protein in SMART SM00454, SAM, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit |
PROSITEi | View protein in PROSITE PS50105, SAM_DOMAIN, 1 hit PS51024, ZF_FCS, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8CHP6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDSEPSSGTS VSTTASSTTT TTITTSSSRM QQPQISVYSG SDRHAVQVIQ
60 70 80 90 100
QALHRPPSSA AQYLQQMYAA QQQHLMLHTA ALQQQHLSSS QLQSLAAVQA
110 120 130 140 150
SLSSGRPSTS PTGSVTQQSS MSQTSILSAS PAPAQLMNRS QTSSSTSGSI
160 170 180 190 200
TQQTMLLGST SPTLTASQAQ MYLRAQMLIF TPATTVAAVQ SDIPVVSSSP
210 220 230 240 250
SPSCQSAAAQ VQNLTLRSQK LGVLSSSQNG SPKSAGQTQS LTICHNKTTV
260 270 280 290 300
TSSKISQRDP SPESKKGGSP GLESRSTAVT RTSSIHQLIA PASYSPIQPH
310 320 330 340 350
SLIKHQQIPL HSPPPKVSHH QLLLQQQQQQ IQPITLQSPS QDPPPSQHCI
360 370 380 390 400
PLPNHGLSPA PSNAQPQHCS PVQSHPPPLT VSPNQAQSAQ QSVVVSPPPP
410 420 430 440 450
HSPSQSPTII IHPQALIQPH PLVSSALQTG PNLQQAAADQ VQSTAQLNLP
460 470 480 490 500
SHLPLPASPV VHIGPVQQSA LVSPGQQMVS PTSHQQYSAL QSSPIPIATP
510 520 530 540 550
PQMSASPPAQ LPPLPLQSMQ SLQVQPEILS QGQVLVQNAL VSEEELPAAE
560 570 580 590 600
ALVQLPFQTL PPPQTVAVNL QVQPPAPVDP PVVYQVEDVC EEEMPEESDE
610 620 630 640 650
CARMDRTPPP PTLSPAAVTV GRGEDLTSEH PLLEQVELPA VASVSASVIK
660 670 680 690 700
SPSDPTHASA PAPPLLIPAA STRSSSTSLA SSTPSLENKP PQAIVKPQIL
710 720 730 740 750
THVIEGFVIQ EGLEPFPVSR SSLLIEQPVK KRPLLDNQVV NSVCVQPELQ
760 770 780 790 800
NNTKHADNSS DTEIEDMMAE ETLEEMDSEL LKCEFCGKMG YPNEFLRSKR
810 820 830 840 850
FCTMSCAKRY NVSCSKKFAL SRWNRKPDNQ SLGHRGRRPS GPEGAAREHI
860 870 880 890 900
LRQLPITYPS AEEDVASHED PVPSAMTTRL RRQSERERER ELRDVRIRKM
910 920 930 940 950
PENSDLLPVA QTEPSIWTVD DVWAFIHSLP GCQDVADEFR AQEIDGQALL
960 970 980
LLKEDHLMSA MNMKLGPALK ICARINSLKD S
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB7ZNA5 | B7ZNA5_MOUSE | Phc3 protein | Phc3 | 951 | Annotation score: | ||
D3YY34 | D3YY34_MOUSE | Polyhomeotic-like protein 3 | Phc3 | 948 | Annotation score: | ||
E9QPT4 | E9QPT4_MOUSE | Polyhomeotic-like protein 3 | Phc3 | 715 | Annotation score: | ||
F8WIN6 | F8WIN6_MOUSE | Polyhomeotic-like protein 3 | Phc3 | 595 | Annotation score: | ||
D6REI6 | D6REI6_MOUSE | Polyhomeotic-like protein 3 | Phc3 | 232 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 147 | S → I in CAC93885 (PubMed:12384788).Curated | 1 | |
Sequence conflicti | 384 | N → T in BAE38687 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 437 | A → T in BAE38687 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 534 | V → G in BAE38687 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 867 | S → F in CAC93885 (PubMed:12384788).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016772 | 1 | M → MAEAEFKDHSTAM in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_016773 | 178 – 210 | Missing in isoform 2. 1 PublicationAdd BLAST | 33 | |
Alternative sequenceiVSP_016774 | 720 – 736 | RSSLL…RPLLD → QKLICVFKIIFSDTTLS in isoform 2. 1 PublicationAdd BLAST | 17 | |
Alternative sequenceiVSP_016775 | 737 – 981 | Missing in isoform 2. 1 PublicationAdd BLAST | 245 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ414610 mRNA Translation: CAC93885.1 AK166296 mRNA Translation: BAE38687.1 BC132533 mRNA Translation: AAI32534.1 |
CCDSi | CCDS17288.1 [Q8CHP6-1] |
RefSeqi | NP_001159426.1, NM_001165954.1 NP_700470.2, NM_153421.2 [Q8CHP6-1] |
Genome annotation databases
Ensembli | ENSMUST00000129817; ENSMUSP00000114916; ENSMUSG00000037652 [Q8CHP6-1] ENSMUST00000168645; ENSMUSP00000130142; ENSMUSG00000037652 [Q8CHP6-1] |
GeneIDi | 241915 |
KEGGi | mmu:241915 |
UCSCi | uc008ovl.2, mouse [Q8CHP6-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ414610 mRNA Translation: CAC93885.1 AK166296 mRNA Translation: BAE38687.1 BC132533 mRNA Translation: AAI32534.1 |
CCDSi | CCDS17288.1 [Q8CHP6-1] |
RefSeqi | NP_001159426.1, NM_001165954.1 NP_700470.2, NM_153421.2 [Q8CHP6-1] |
3D structure databases
SMRi | Q8CHP6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 232356, 6 interactors |
IntActi | Q8CHP6, 3 interactors |
MINTi | Q8CHP6 |
STRINGi | 10090.ENSMUSP00000130142 |
PTM databases
iPTMneti | Q8CHP6 |
PhosphoSitePlusi | Q8CHP6 |
Proteomic databases
EPDi | Q8CHP6 |
jPOSTi | Q8CHP6 |
PaxDbi | Q8CHP6 |
PRIDEi | Q8CHP6 |
Protocols and materials databases
Antibodypediai | 33708, 102 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000129817; ENSMUSP00000114916; ENSMUSG00000037652 [Q8CHP6-1] ENSMUST00000168645; ENSMUSP00000130142; ENSMUSG00000037652 [Q8CHP6-1] |
GeneIDi | 241915 |
KEGGi | mmu:241915 |
UCSCi | uc008ovl.2, mouse [Q8CHP6-1] |
Organism-specific databases
CTDi | 80012 |
MGIi | MGI:2181434, Phc3 |
Phylogenomic databases
eggNOGi | ENOG502QS5Q, Eukaryota |
GeneTreei | ENSGT00940000154964 |
InParanoidi | Q8CHP6 |
OMAi | ANCNQAQ |
OrthoDBi | 298184at2759 |
PhylomeDBi | Q8CHP6 |
TreeFami | TF331299 |
Enzyme and pathway databases
Reactomei | R-MMU-3108214, SUMOylation of DNA damage response and repair proteins R-MMU-3899300, SUMOylation of transcription cofactors R-MMU-4551638, SUMOylation of chromatin organization proteins R-MMU-4570464, SUMOylation of RNA binding proteins R-MMU-8939243, RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known R-MMU-8943724, Regulation of PTEN gene transcription R-MMU-8953750, Transcriptional Regulation by E2F6 |
Miscellaneous databases
BioGRID-ORCSi | 241915, 5 hits in 19 CRISPR screens |
ChiTaRSi | Phc3, mouse |
PROi | PR:Q8CHP6 |
RNActi | Q8CHP6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037652, Expressed in mesenteric lymph node and 257 other tissues |
Genevisiblei | Q8CHP6, MM |
Family and domain databases
Gene3Di | 1.10.150.50, 1 hit 3.30.60.160, 1 hit |
InterProi | View protein in InterPro IPR001660, SAM IPR013761, SAM/pointed_sf IPR012313, Znf_FCS IPR038603, Znf_FCS_sf |
Pfami | View protein in Pfam PF00536, SAM_1, 1 hit |
SMARTi | View protein in SMART SM00454, SAM, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit |
PROSITEi | View protein in PROSITE PS50105, SAM_DOMAIN, 1 hit PS51024, ZF_FCS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PHC3_MOUSE | |
Accessioni | Q8CHP6Primary (citable) accession number: Q8CHP6 Secondary accession number(s): A2RTJ9, Q3TLV5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 136 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot