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Protein

Histone acetyltransferase KAT5

Gene

Kat5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. Relieves NR1D2-mediated inhibition of APOC3 expression by acetylating NR1D2. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. Promotes FOXP3 acetylation and positively regulates its transcriptional repressor activity. Acetylates RAN at 'Lys-134'.By similarity

Catalytic activityi

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei403Proton donor/acceptorBy similarity1
Binding sitei407Acetyl-CoABy similarity1
Binding sitei416Acetyl-CoABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri260 – 285C2HC MYST-typePROSITE-ProRule annotationAdd BLAST26

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Acyltransferase, Chromatin regulator, Transferase
Biological processGrowth regulation, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-201722 Formation of the beta-catenin:TCF transactivating complex
R-MMU-2559586 DNA Damage/Telomere Stress Induced Senescence
R-MMU-5685938 HDR through Single Strand Annealing (SSA)
R-MMU-5685942 HDR through Homologous Recombination (HRR)
R-MMU-5693548 Sensing of DNA Double Strand Breaks
R-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-MMU-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-MMU-5693571 Nonhomologous End-Joining (NHEJ)
R-MMU-5693579 Homologous DNA Pairing and Strand Exchange
R-MMU-5693607 Processing of DNA double-strand break ends
R-MMU-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-69473 G2/M DNA damage checkpoint
R-MMU-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
Histone acetyltransferase KAT5 (EC:2.3.1.48By similarity)
Alternative name(s):
60 kDa Tat-interactive protein
Short name:
Tip60
Histone acetyltransferase HTATIP
Lysine acetyltransferase 5
Gene namesi
Name:Kat5
Synonyms:Htatip, Tip60
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1932051 Kat5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000515811 – 513Histone acetyltransferase KAT5Add BLAST513

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52N6-acetyllysineBy similarity1
Modified residuei86PhosphoserineBy similarity1
Modified residuei90Phosphoserine; by CDK1By similarity1
Modified residuei199PhosphoserineCombined sources1
Modified residuei327N6-acetyllysine; by autocatalysisBy similarity1
Cross-linki430Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki451Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

Sumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies.By similarity
Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. The phosphorylated form has a higher HAT activity.By similarity
Ubiquitinated by MDM2, leading to its proteasome-dependent degradation.By similarity
Autoacetylation at Lys-327 is required for proper function.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ8CHK4
PeptideAtlasiQ8CHK4
PRIDEiQ8CHK4

PTM databases

iPTMnetiQ8CHK4
PhosphoSitePlusiQ8CHK4

Expressioni

Tissue specificityi

Expressed in testis, heart, brain, kidney and liver. Weakly expressed in lung.1 Publication

Gene expression databases

BgeeiENSMUSG00000024926 Expressed in 262 organ(s), highest expression level in cerebellum
ExpressionAtlasiQ8CHK4 baseline and differential
GenevisibleiQ8CHK4 MM

Interactioni

Subunit structurei

Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. HTATTIP/TIP60, EPC1, and ING3 together constitute a minimal HAT complex termed Piccolo NuA4. The NuA4 complex interacts with MYC. Interacts with ATM. Interacts with JADE1. Interacts with PLA2G4A/CPLA2, EDNRA and HDAC7. Interacts with the cytoplasmic tail of APP and APBB1/FE65. Interacts with TRIM24 and TRIM68. Forms a complex with SENP6 and UBE2I in response to UV irradiation. Identified in a complex with HINT1. Interacts with ATF2 and CUL3. Interacts with NR1D2 (via N-terminus). Component of a SWR1-like complex (By similarity). Interacts with FOXP3. Interacts with ZBTB49 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Atxn1P542542EBI-1169948,EBI-1169713

GO - Molecular functioni

Protein-protein interaction databases

BioGridi219884, 59 interactors
ComplexPortaliCPX-747 Piccolo NuA4 histone acetyltransferase complex
CPX-990 NuA4 histone acetyltransferase complex
IntActiQ8CHK4, 24 interactors
MINTiQ8CHK4
STRINGi10090.ENSMUSP00000109271

Structurei

3D structure databases

ProteinModelPortaliQ8CHK4
SMRiQ8CHK4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini227 – 504MYST-type HATPROSITE-ProRule annotationAdd BLAST278

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni368 – 513Interaction with ATF2By similarityAdd BLAST146
Regioni370 – 372Acetyl-CoA bindingBy similarity3
Regioni377 – 383Acetyl-CoA bindingBy similarity7

Sequence similaritiesi

Belongs to the MYST (SAS/MOZ) family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri260 – 285C2HC MYST-typePROSITE-ProRule annotationAdd BLAST26

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2747 Eukaryota
COG5027 LUCA
GeneTreeiENSGT00930000150839
HOGENOMiHOG000182457
InParanoidiQ8CHK4
KOiK11304
OMAiKMQVYHK
OrthoDBiEOG091G0B73
PhylomeDBiQ8CHK4
TreeFamiTF317619

Family and domain databases

CDDicd00024 CHROMO, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR016197 Chromo-like_dom_sf
IPR000953 Chromo/chromo_shadow_dom
IPR037995 Esa1/KAT5/Tip60
IPR002717 HAT_MYST-type
IPR025995 Tudor-knot
IPR036388 WH-like_DNA-bd_sf
PANTHERiPTHR10615:SF124 PTHR10615:SF124, 1 hit
PfamiView protein in Pfam
PF01853 MOZ_SAS, 1 hit
PF11717 Tudor-knot, 1 hit
SMARTiView protein in SMART
SM00298 CHROMO, 1 hit
SUPFAMiSSF54160 SSF54160, 1 hit
SSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS51726 MYST_HAT, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q8CHK4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAEVGEIIEG CRLPVLRRNQ DNEDEWPLAE ILSVKDISGR KLFYVHYIDF
60 70 80 90 100
NKRLDEWVTH ERLDLKKIQF PKKEAKTPTK NGLPGSRPGS PEREVPASAQ
110 120 130 140 150
ASGKTLPIPV QITLRFNLPK EREAIPGGEP DQPLSSSSCL QPNHRSTKRK
160 170 180 190 200
VEVVSPATPV PSETAPASVF PQNGSARRAV AAQPGRKRKS NCLGTDEDSQ
210 220 230 240 250
DSSDGIPSAP RMTGSLVSDR SHDDIVTRMK NIECIELGRH RLKPWYFSPY
260 270 280 290 300
PQELTTLPVL YLCEFCLKYG RSLKCLQRHL TKCDLRHPPG NEIYRKGTIS
310 320 330 340 350
FFEIDGRKNK SYSQNLCLLA KCFLDHKTLY YDTDPFLFYV MTEYDCKGFH
360 370 380 390 400
IVGYFSKEKE STEDYNVACI LTLPPYQRRG YGKLLIEFSY ELSKVEGKTG
410 420 430 440 450
TPEKPLSDLG LLSYRSYWSQ TILEILMGLK SESGERPQIT INEISEITSI
460 470 480 490 500
KKEDVISTLQ YLNLINYYKG QYILTLSEDI VDGHERAMLK RLLRIDSKCL
510
HFTPKDWSKR GKW
Length:513
Mass (Da):58,598
Last modified:November 28, 2003 - v2
Checksum:iEACEE4D544C0DB60
GO
Isoform 2 (identifier: Q8CHK4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     96-147: Missing.

Show »
Length:461
Mass (Da):53,093
Checksum:iD3B238AF01737EF3
GO
Isoform 3 (identifier: Q8CHK4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     4-4: V → VVSPVPGAGRREPGEVGRARGPPVADPGVALSPQ

Show »
Length:546
Mass (Da):61,814
Checksum:i8557B105D8F4B291
GO
Isoform 4 (identifier: Q8CHK4-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-211: Missing.

Note: No experimental confirmation available.
Show »
Length:302
Mass (Da):35,396
Checksum:iD96A7FCCB49FA85D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0091051 – 211Missing in isoform 4. 1 PublicationAdd BLAST211
Alternative sequenceiVSP_0091064V → VVSPVPGAGRREPGEVGRAR GPPVADPGVALSPQ in isoform 3. Curated1
Alternative sequenceiVSP_00910796 – 147Missing in isoform 2. 2 PublicationsAdd BLAST52

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061983 Genomic DNA Translation: AAL34981.1
AF528194 mRNA Translation: AAN77140.1
AF528195 mRNA Translation: AAN77141.1
AF528196 mRNA Translation: AAN77142.1
AB055409 mRNA Translation: BAC53807.1
BC129968 mRNA Translation: AAI29969.1
CCDSiCCDS29472.1 [Q8CHK4-1]
RefSeqiNP_001186176.1, NM_001199247.1 [Q8CHK4-2]
NP_001186177.1, NM_001199248.1
NP_848752.1, NM_178637.2 [Q8CHK4-1]
UniGeneiMm.228930

Genome annotation databases

EnsembliENSMUST00000113641; ENSMUSP00000109271; ENSMUSG00000024926 [Q8CHK4-1]
GeneIDi81601
KEGGimmu:81601
UCSCiuc008gdy.2 mouse [Q8CHK4-1]
uc008geb.2 mouse [Q8CHK4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061983 Genomic DNA Translation: AAL34981.1
AF528194 mRNA Translation: AAN77140.1
AF528195 mRNA Translation: AAN77141.1
AF528196 mRNA Translation: AAN77142.1
AB055409 mRNA Translation: BAC53807.1
BC129968 mRNA Translation: AAI29969.1
CCDSiCCDS29472.1 [Q8CHK4-1]
RefSeqiNP_001186176.1, NM_001199247.1 [Q8CHK4-2]
NP_001186177.1, NM_001199248.1
NP_848752.1, NM_178637.2 [Q8CHK4-1]
UniGeneiMm.228930

3D structure databases

ProteinModelPortaliQ8CHK4
SMRiQ8CHK4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219884, 59 interactors
ComplexPortaliCPX-747 Piccolo NuA4 histone acetyltransferase complex
CPX-990 NuA4 histone acetyltransferase complex
IntActiQ8CHK4, 24 interactors
MINTiQ8CHK4
STRINGi10090.ENSMUSP00000109271

PTM databases

iPTMnetiQ8CHK4
PhosphoSitePlusiQ8CHK4

Proteomic databases

PaxDbiQ8CHK4
PeptideAtlasiQ8CHK4
PRIDEiQ8CHK4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113641; ENSMUSP00000109271; ENSMUSG00000024926 [Q8CHK4-1]
GeneIDi81601
KEGGimmu:81601
UCSCiuc008gdy.2 mouse [Q8CHK4-1]
uc008geb.2 mouse [Q8CHK4-2]

Organism-specific databases

CTDi10524
MGIiMGI:1932051 Kat5

Phylogenomic databases

eggNOGiKOG2747 Eukaryota
COG5027 LUCA
GeneTreeiENSGT00930000150839
HOGENOMiHOG000182457
InParanoidiQ8CHK4
KOiK11304
OMAiKMQVYHK
OrthoDBiEOG091G0B73
PhylomeDBiQ8CHK4
TreeFamiTF317619

Enzyme and pathway databases

ReactomeiR-MMU-201722 Formation of the beta-catenin:TCF transactivating complex
R-MMU-2559586 DNA Damage/Telomere Stress Induced Senescence
R-MMU-5685938 HDR through Single Strand Annealing (SSA)
R-MMU-5685942 HDR through Homologous Recombination (HRR)
R-MMU-5693548 Sensing of DNA Double Strand Breaks
R-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-MMU-5693568 Resolution of D-loop Structures through Holliday Junction Intermediates
R-MMU-5693571 Nonhomologous End-Joining (NHEJ)
R-MMU-5693579 Homologous DNA Pairing and Strand Exchange
R-MMU-5693607 Processing of DNA double-strand break ends
R-MMU-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-69473 G2/M DNA damage checkpoint
R-MMU-9018519 Estrogen-dependent gene expression

Miscellaneous databases

ChiTaRSiKat5 mouse
PROiPR:Q8CHK4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024926 Expressed in 262 organ(s), highest expression level in cerebellum
ExpressionAtlasiQ8CHK4 baseline and differential
GenevisibleiQ8CHK4 MM

Family and domain databases

CDDicd00024 CHROMO, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR016197 Chromo-like_dom_sf
IPR000953 Chromo/chromo_shadow_dom
IPR037995 Esa1/KAT5/Tip60
IPR002717 HAT_MYST-type
IPR025995 Tudor-knot
IPR036388 WH-like_DNA-bd_sf
PANTHERiPTHR10615:SF124 PTHR10615:SF124, 1 hit
PfamiView protein in Pfam
PF01853 MOZ_SAS, 1 hit
PF11717 Tudor-knot, 1 hit
SMARTiView protein in SMART
SM00298 CHROMO, 1 hit
SUPFAMiSSF54160 SSF54160, 1 hit
SSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS51726 MYST_HAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKAT5_MOUSE
AccessioniPrimary (citable) accession number: Q8CHK4
Secondary accession number(s): A1L394
, Q8CGZ3, Q8CGZ4, Q8VIH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: November 7, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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