UniProtKB - Q8CH18 (CCAR1_MOUSE)
Protein
Cell division cycle and apoptosis regulator protein 1
Gene
Ccar1
Organism
Mus musculus (Mouse)
Status
Functioni
Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation. May be involved in apoptosis signaling in the presence of the retinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation (By similarity). In association with CALCOCO1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781). Can act as a both a coactivator and corepressor of AR-mediated transcription. Contributes to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites. May play an important role in the growth and tumorigenesis of prostate cancer cells (PubMed:23887938).By similarity2 Publications
GO - Molecular functioni
- nuclear receptor transcription coactivator activity Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- transcription coactivator activity Source: UniProtKB
- transcription corepressor activity Source: UniProtKB
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- cell cycle Source: UniProtKB-KW
- positive regulation of apoptotic process Source: MGI
- positive regulation of cell migration Source: UniProtKB
- positive regulation of cell population proliferation Source: UniProtKB
- regulation of transcription, DNA-templated Source: GO_Central
Keywordsi
Molecular function | Activator, Repressor |
Biological process | Apoptosis, Cell cycle, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Cell division cycle and apoptosis regulator protein 1Alternative name(s): Cell cycle and apoptosis regulatory protein 1 Short name: CARP-1 |
Gene namesi | Name:Ccar1 Synonyms:Carp1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914750, Ccar1 |
Subcellular locationi
Other locations
- perinuclear region By similarity
Nucleus
- nuclear envelope lumen Source: MGI
- nucleus Source: GO_Central
Other locations
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000233149 | 1 – 1146 | Cell division cycle and apoptosis regulator protein 1Add BLAST | 1146 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 453 | PhosphoserineBy similarity | 1 | |
Modified residuei | 624 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 634 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 664 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 682 | PhosphoserineBy similarity | 1 | |
Modified residuei | 694 | PhosphoserineBy similarity | 1 | |
Modified residuei | 858 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 1008 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 1008 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Cross-linki | 1063 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 1131 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8CH18 |
jPOSTi | Q8CH18 |
PaxDbi | Q8CH18 |
PeptideAtlasi | Q8CH18 |
PRIDEi | Q8CH18 |
PTM databases
iPTMneti | Q8CH18 |
PhosphoSitePlusi | Q8CH18 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000020074, Expressed in pes and 279 other tissues |
Genevisiblei | Q8CH18, MM |
Interactioni
Subunit structurei
Directly interacts with ESR1, NR3C1 and p53/TP53.
Interacts (via N-terminus) with CALCOCO1.
Interacts with MED1 and GATA1.
Interacts with AR and GATA2 (By similarity).
By similarity2 PublicationsProtein-protein interaction databases
BioGRIDi | 212231, 9 interactors |
IntActi | Q8CH18, 6 interactors |
MINTi | Q8CH18 |
STRINGi | 10090.ENSMUSP00000020268 |
Miscellaneous databases
RNActi | Q8CH18, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 633 – 667 | SAPPROSITE-ProRule annotationAdd BLAST | 35 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 246 | Interaction with ARBy similarityAdd BLAST | 246 | |
Regioni | 200 – 657 | Interaction with GATA2By similarityAdd BLAST | 458 | |
Regioni | 640 – 1146 | Interaction with GATA11 PublicationAdd BLAST | 507 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 591 – 615 | Sequence analysisAdd BLAST | 25 | |
Coiled coili | 1029 – 1110 | Sequence analysisAdd BLAST | 82 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 3 – 287 | Gln-richAdd BLAST | 285 | |
Compositional biasi | 290 – 358 | Arg-richAdd BLAST | 69 | |
Compositional biasi | 670 – 886 | Glu-richAdd BLAST | 217 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG4246, Eukaryota |
GeneTreei | ENSGT00530000063672 |
HOGENOMi | CLU_008030_0_0_1 |
InParanoidi | Q8CH18 |
OMAi | LRYSDMH |
OrthoDBi | 614048at2759 |
PhylomeDBi | Q8CH18 |
TreeFami | TF316387 |
Family and domain databases
Gene3Di | 1.10.720.30, 1 hit |
InterProi | View protein in InterPro IPR025224, CCAR1/CCAR2 IPR025954, DBC1/CARP1_inactive_NUDIX_dom IPR011992, EF-hand-dom_pair IPR025223, S1-like_RNA-bd_dom IPR003034, SAP_dom IPR036361, SAP_dom_sf |
PANTHERi | PTHR14304, PTHR14304, 1 hit |
Pfami | View protein in Pfam PF14443, DBC1, 1 hit PF14444, S1-like, 1 hit PF02037, SAP, 1 hit |
SMARTi | View protein in SMART SM01122, DBC1, 1 hit SM00513, SAP, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF68906, SSF68906, 1 hit |
PROSITEi | View protein in PROSITE PS50800, SAP, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8CH18-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAQFGGQKNP PWATQFTATA VSQPAALGVQ QPSLLGASPT IYTQQTALAA
60 70 80 90 100
AGLTTQTPAN YQLTQTAALQ QQAAAVLQQQ YSQPQQALYS VQQQLQQPQQ
110 120 130 140 150
TILTQPAVAL PTSLSLSTPQ PAAQITVSYP TPRSSQQQTQ PQKQRVFTGV
160 170 180 190 200
VTKLHDTFGF VDEDVFFQLG AVKGKTPQVG DRVLVEATYN PNMPFKWNAQ
210 220 230 240 250
RIQTLPNQNQ SQTQPLLKTP TAVIQPIVPQ TTFGVQAQPQ PQSLLQAQIS
260 270 280 290 300
AASITPLLQT QPQPLLQQPQ QKAGLLQPPV RIVSQPQPAR RLDPPSRFSG
310 320 330 340 350
RNDRGDQVPN RKDDRSRERD RERRRSRERS PQRKRSRERS PRRERERSPR
360 370 380 390 400
RVRRVVPRYT VQFSKFSLDC PSCDMMELRR RYQNLYIPSD FFDAQFTWVD
410 420 430 440 450
AFPLSRPFQL GNYCNFYVMH REVESLEKNM AVLDPPDADH LYSAKVMLMA
460 470 480 490 500
SPSMEDLYHK SCALAEDPQD LRDGFQHPAR LVKFLVGMKG KDEAMAIGGH
510 520 530 540 550
WSPSLDGPNP EKDPSVLIKT AIRCCKALTG IDLSVCTQWY RFAEIRYHRP
560 570 580 590 600
EETHKGRTVP AHVETVVLFF PDVWHCLPTR SEWETLSRGY KQQLVEKLQG
610 620 630 640 650
ERKKADGEQD EEEKDDGEVK EIATPTHWSK LDPKAMKVND LRKELESRAL
660 670 680 690 700
SSKGLKSQLI ARLTKQLKIE EQKEEQKELE KSEKEEEDED DKKSEDDKEE
710 720 730 740 750
EERKRQEEVE RQRQERRYIL PDEPAIIVHP NWAAKSGKFD CSIMSLSVLL
760 770 780 790 800
DYRLEDNKEH SFEVSLFAEL FNEMLQRDFG VRIYKSLLSL PEKEDKKDKE
810 820 830 840 850
KKSKKEERKD KKEEREDDID EPKPKRRKSG DDKDKKEDRD ERKKEEKRKD
860 870 880 890 900
DSKDDDETEE DNNQDEYDPM EAEEAEDEDD DREEEEVKRD DKRDVSRYCK
910 920 930 940 950
DRPAKDKEKE KPQMVTVNRD LLMAFVYFDQ SHCGYLLEKD LEEILYTLGL
960 970 980 990 1000
HLSRAQVKKL LNKVVLRESC FYRKLTDTSK DDENHEESEA LQEDMLGNRL
1010 1020 1030 1040 1050
LLPTPTIKQE SKDGEENVGL IVYNGAMVDV GSLLQKLEKS EKVRAEVEQK
1060 1070 1080 1090 1100
LQLLEEKTDE DGKTILNLEN SNKSLSGELR EVKKDLGQLQ ENLEVSENMN
1110 1120 1130 1140
LQFENQLNKT LRNLSTVMDD IHTVLKKDNV KSEDRDEKSK ENGSGV
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2P765 | A0A1W2P765_MOUSE | Cell division cycle and apoptosis r... | Ccar1 | 198 | Annotation score: | ||
A0A1W2P7Q7 | A0A1W2P7Q7_MOUSE | Cell division cycle and apoptosis r... | Ccar1 | 161 | Annotation score: | ||
A0A1W2P6V0 | A0A1W2P6V0_MOUSE | Cell division cycle and apoptosis r... | Ccar1 | 92 | Annotation score: |
Sequence cautioni
The sequence AAH10199 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH34174 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH39939 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH51052 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH60130 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH79652 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 279 | P → L in AAH79652 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 558 | T → A in AAH39939 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 802 | K → E in BAC37267 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 821 | E → G in BAC37267 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018054 | 316 – 327 | SRERD…RRRSR → RYVLRQCGGLEK in isoform 2. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_018055 | 328 – 1146 | Missing in isoform 2. 1 PublicationAdd BLAST | 819 | |
Alternative sequenceiVSP_037737 | 638 – 699 | Missing in isoform 3. 1 PublicationAdd BLAST | 62 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF465615 mRNA Translation: AAO17318.1 AK012111 mRNA Translation: BAB28040.2 AK077151 mRNA Translation: BAC36646.1 AK078424 mRNA Translation: BAC37267.1 BC010199 mRNA Translation: AAH10199.1 Sequence problems. BC034174 mRNA Translation: AAH34174.1 Sequence problems. BC039939 mRNA Translation: AAH39939.1 Sequence problems. BC051052 mRNA Translation: AAH51052.1 Sequence problems. BC060130 mRNA Translation: AAH60130.1 Sequence problems. BC079652 mRNA Translation: AAH79652.1 Sequence problems. |
CCDSi | CCDS35922.1 [Q8CH18-1] |
RefSeqi | NP_080477.1, NM_026201.3 [Q8CH18-1] XP_006514057.1, XM_006513994.3 [Q8CH18-1] XP_006514058.1, XM_006513995.3 [Q8CH18-1] XP_006514059.1, XM_006513996.3 [Q8CH18-1] XP_006514060.1, XM_006513997.2 [Q8CH18-3] XP_011241839.1, XM_011243537.2 [Q8CH18-1] |
Genome annotation databases
Ensembli | ENSMUST00000020268; ENSMUSP00000020268; ENSMUSG00000020074 [Q8CH18-1] ENSMUST00000219527; ENSMUSP00000151895; ENSMUSG00000020074 [Q8CH18-1] |
GeneIDi | 67500 |
KEGGi | mmu:67500 |
UCSCi | uc007fja.2, mouse [Q8CH18-1] uc007fjc.2, mouse [Q8CH18-2] uc011xff.1, mouse [Q8CH18-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF465615 mRNA Translation: AAO17318.1 AK012111 mRNA Translation: BAB28040.2 AK077151 mRNA Translation: BAC36646.1 AK078424 mRNA Translation: BAC37267.1 BC010199 mRNA Translation: AAH10199.1 Sequence problems. BC034174 mRNA Translation: AAH34174.1 Sequence problems. BC039939 mRNA Translation: AAH39939.1 Sequence problems. BC051052 mRNA Translation: AAH51052.1 Sequence problems. BC060130 mRNA Translation: AAH60130.1 Sequence problems. BC079652 mRNA Translation: AAH79652.1 Sequence problems. |
CCDSi | CCDS35922.1 [Q8CH18-1] |
RefSeqi | NP_080477.1, NM_026201.3 [Q8CH18-1] XP_006514057.1, XM_006513994.3 [Q8CH18-1] XP_006514058.1, XM_006513995.3 [Q8CH18-1] XP_006514059.1, XM_006513996.3 [Q8CH18-1] XP_006514060.1, XM_006513997.2 [Q8CH18-3] XP_011241839.1, XM_011243537.2 [Q8CH18-1] |
3D structure databases
SMRi | Q8CH18 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 212231, 9 interactors |
IntActi | Q8CH18, 6 interactors |
MINTi | Q8CH18 |
STRINGi | 10090.ENSMUSP00000020268 |
PTM databases
iPTMneti | Q8CH18 |
PhosphoSitePlusi | Q8CH18 |
Proteomic databases
EPDi | Q8CH18 |
jPOSTi | Q8CH18 |
PaxDbi | Q8CH18 |
PeptideAtlasi | Q8CH18 |
PRIDEi | Q8CH18 |
Protocols and materials databases
Antibodypediai | 2118, 192 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000020268; ENSMUSP00000020268; ENSMUSG00000020074 [Q8CH18-1] ENSMUST00000219527; ENSMUSP00000151895; ENSMUSG00000020074 [Q8CH18-1] |
GeneIDi | 67500 |
KEGGi | mmu:67500 |
UCSCi | uc007fja.2, mouse [Q8CH18-1] uc007fjc.2, mouse [Q8CH18-2] uc011xff.1, mouse [Q8CH18-3] |
Organism-specific databases
CTDi | 55749 |
MGIi | MGI:1914750, Ccar1 |
Phylogenomic databases
eggNOGi | KOG4246, Eukaryota |
GeneTreei | ENSGT00530000063672 |
HOGENOMi | CLU_008030_0_0_1 |
InParanoidi | Q8CH18 |
OMAi | LRYSDMH |
OrthoDBi | 614048at2759 |
PhylomeDBi | Q8CH18 |
TreeFami | TF316387 |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway |
Miscellaneous databases
BioGRID-ORCSi | 67500, 5 hits in 17 CRISPR screens |
ChiTaRSi | Ccar1, mouse |
PROi | PR:Q8CH18 |
RNActi | Q8CH18, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020074, Expressed in pes and 279 other tissues |
Genevisiblei | Q8CH18, MM |
Family and domain databases
Gene3Di | 1.10.720.30, 1 hit |
InterProi | View protein in InterPro IPR025224, CCAR1/CCAR2 IPR025954, DBC1/CARP1_inactive_NUDIX_dom IPR011992, EF-hand-dom_pair IPR025223, S1-like_RNA-bd_dom IPR003034, SAP_dom IPR036361, SAP_dom_sf |
PANTHERi | PTHR14304, PTHR14304, 1 hit |
Pfami | View protein in Pfam PF14443, DBC1, 1 hit PF14444, S1-like, 1 hit PF02037, SAP, 1 hit |
SMARTi | View protein in SMART SM01122, DBC1, 1 hit SM00513, SAP, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF68906, SSF68906, 1 hit |
PROSITEi | View protein in PROSITE PS50800, SAP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CCAR1_MOUSE | |
Accessioni | Q8CH18Primary (citable) accession number: Q8CH18 Secondary accession number(s): Q05BR1 Q9CSR5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot