UniProtKB - Q8CGU6 (NICA_RAT)
Nicastrin
Ncstn
Functioni
GO - Molecular functioni
- ATPase binding Source: RGD
- endopeptidase activity Source: Ensembl
- growth factor receptor binding Source: RGD
- peptidase activity Source: RGD
GO - Biological processi
- adult behavior Source: RGD
- amyloid-beta formation Source: UniProtKB
- amyloid-beta metabolic process Source: RGD
- amyloid precursor protein biosynthetic process Source: RGD
- amyloid precursor protein metabolic process Source: UniProtKB
- cellular response to calcium ion Source: RGD
- central nervous system myelination Source: RGD
- cerebellum development Source: RGD
- dopamine receptor signaling pathway Source: RGD
- epithelial cell proliferation Source: RGD
- glutamate receptor signaling pathway Source: RGD
- learning or memory Source: RGD
- membrane protein ectodomain proteolysis Source: UniProtKB
- myeloid cell homeostasis Source: RGD
- neuron apoptotic process Source: RGD
- neuron death Source: RGD
- Notch receptor processing Source: GO_Central
- Notch signaling pathway Source: UniProtKB-KW
- positive regulation of amyloid precursor protein biosynthetic process Source: RGD
- positive regulation of catalytic activity Source: UniProtKB
- protein processing Source: RGD
- regulation of long-term synaptic potentiation Source: RGD
- short-term synaptic potentiation Source: RGD
- T cell proliferation Source: RGD
Keywordsi
Biological process | Notch signaling pathway |
Enzyme and pathway databases
Reactomei | R-RNO-1251985, Nuclear signaling by ERBB4 R-RNO-193692, Regulated proteolysis of p75NTR R-RNO-205043, NRIF signals cell death from the nucleus R-RNO-3928665, EPH-ephrin mediated repulsion of cells R-RNO-6798695, Neutrophil degranulation R-RNO-9013507, NOTCH3 Activation and Transmission of Signal to the Nucleus R-RNO-9017802, Noncanonical activation of NOTCH3 |
Names & Taxonomyi
Protein namesi | Recommended name: Nicastrin |
Gene namesi | Name:Ncstn |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 631418, Ncstn |
Subcellular locationi
Other locations
- Membrane By similarity; Single-pass type I membrane protein By similarity
- Cytoplasmic vesicle membrane By similarity; Single-pass type I membrane protein By similarity
- Melanosome By similarity
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
Endosome
- early endosome Source: RGD
Golgi apparatus
- Golgi apparatus Source: UniProtKB
Lysosome
- lysosomal membrane Source: RGD
- lysosome Source: RGD
Mitochondrion
- mitochondrion Source: RGD
Plasma Membrane
- gamma-secretase complex Source: UniProtKB
- integral component of plasma membrane Source: UniProtKB
- integral component of presynaptic membrane Source: SynGO
- plasma membrane Source: RGD
- sarcolemma Source: RGD
- synaptic membrane Source: RGD
Other locations
- cytoplasm Source: RGD
- cytoplasmic vesicle membrane Source: UniProtKB-SubCell
- integral component of membrane Source: UniProtKB
- melanosome Source: UniProtKB-SubCell
- membrane Source: RGD
- protein-containing complex Source: RGD
- synapse Source: RGD
- synaptic vesicle Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 35 – 668 | LumenalBy similarityAdd BLAST | 634 | |
Transmembranei | 669 – 689 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 690 – 708 | CytoplasmicBy similarityAdd BLAST | 19 |
Keywords - Cellular componenti
Cytoplasmic vesicle, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 34 | Sequence analysisAdd BLAST | 34 | |
ChainiPRO_0000019683 | 35 – 708 | NicastrinAdd BLAST | 674 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 44 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 49 ↔ 61 | By similarity | ||
Glycosylationi | 54 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 128 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 139 ↔ 158 | By similarity | ||
Glycosylationi | 186 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 194 ↔ 212 | By similarity | ||
Glycosylationi | 203 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 229 ↔ 247 | By similarity | ||
Glycosylationi | 263 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 386 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 434 | N-linked (GlcNAc...) asparagineCombined sources | 1 | |
Glycosylationi | 463 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 507 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 529 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 561 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 572 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 579 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 585 ↔ 619 | By similarity | ||
Glycosylationi | 593 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 611 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
jPOSTi | Q8CGU6 |
PaxDbi | Q8CGU6 |
PRIDEi | Q8CGU6 |
PTM databases
GlyGeni | Q8CGU6, 16 sites, 7 N-linked glycans (1 site) |
iPTMneti | Q8CGU6 |
PhosphoSitePlusi | Q8CGU6 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000005355, Expressed in jejunum and 20 other tissues |
ExpressionAtlasi | Q8CGU6, baseline and differential |
Genevisiblei | Q8CGU6, RN |
Interactioni
Subunit structurei
Component of the gamma-secretase complex. The functional gamma-secretase complex is composed of at least four polypeptides: a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP).
Interacts with PSEN1 and PSEN2.
By similarityGO - Molecular functioni
- ATPase binding Source: RGD
- growth factor receptor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 252837, 1 interactor |
CORUMi | Q8CGU6 |
IntActi | Q8CGU6, 4 interactors |
STRINGi | 10116.ENSRNOP00000008150 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2657, Eukaryota |
GeneTreei | ENSGT00390000014633 |
HOGENOMi | CLU_024257_0_0_1 |
InParanoidi | Q8CGU6 |
OMAi | SYGSEFA |
OrthoDBi | 777987at2759 |
PhylomeDBi | Q8CGU6 |
TreeFami | TF317086 |
Family and domain databases
InterProi | View protein in InterPro IPR041084, Ncstrn_small IPR008710, Nicastrin |
PANTHERi | PTHR21092, PTHR21092, 1 hit |
Pfami | View protein in Pfam PF18266, Ncstrn_small, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATARGGSGP DPGSRGLLLL SFSVVLAGLC GGNSVERKIY IPLNKTAPCV
60 70 80 90 100
RLLNATHQIG CQSSISGDTG VIHVVEKEDD LKWVLTDGPN PPYMVLLEGK
110 120 130 140 150
LFTRDIMEKL KGETSRIAGL AVTLAKPNST SSFSPSVQCP NDGFGIYSNS
160 170 180 190 200
YGPEFAHCKK TLWNELGNGL AYDDFSFPIF LLEDENETKV IKQCYQDHNL
210 220 230 240 250
GQNGSAPSFP LCAMQLFSHM HAVISTATCM RRSFIQSTFS INPEIVCDPL
260 270 280 290 300
SDYNVWSMLK PINTSGGLEP DVRVVVAATR LDSRSFFWNV APGAESAVAS
310 320 330 340 350
FVTQLAAAEA LHKAPDVTTL PRNVMFVFFQ GETFDYIGSS RMVYDMENGK
360 370 380 390 400
FPVRLENIDS FVELGQVALR TSLELWMHTD PMSQKNESVK NQVEDLLVTL
410 420 430 440 450
EQSGADTPQV VLSRLVQSQA LPPSSLQRFL RARNISGVVL ADHSGSFHNR
460 470 480 490 500
YYQSIYDTAE NINVTYPESQ SPEEDLNFVT DTAKALADVA TVLARALYKL
510 520 530 540 550
AGGTNFNNSI QADPQTVTRL LYGFLVRANN SWFQSILRHD LRSYLDDGPL
560 570 580 590 600
QHYIAVSSPT NTTYVVQYAL ANLTGKVTNL TQEQCQDPSK VPNESKDLYE
610 620 630 640 650
YSWVQGPWNS NKTERLPRCV RSTVRLARAL SPAFELSQWS STEYSTWAES
660 670 680 690 700
RWKDIQARIF LIASKELEFI TLIVGFSILV FSLIVTYCIN AKADVLFVAP
REPGAVSY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketB3DM90 | B3DM90_RAT | Nicastrin | Ncstn rCG_20460 | 602 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008387 | 63 – 708 | Missing in isoform 2. 1 PublicationAdd BLAST | 646 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF510722 mRNA Translation: AAO15915.1 AY101378 mRNA Translation: AAM44078.1 |
RefSeqi | NP_777353.1, NM_174864.3 [Q8CGU6-1] XP_008767955.1, XM_008769733.1 [Q8CGU6-1] |
Genome annotation databases
Ensembli | ENSRNOT00000008150; ENSRNOP00000008150; ENSRNOG00000005355 [Q8CGU6-1] |
GeneIDi | 289231 |
KEGGi | rno:289231 |
UCSCi | RGD:631418, rat [Q8CGU6-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF510722 mRNA Translation: AAO15915.1 AY101378 mRNA Translation: AAM44078.1 |
RefSeqi | NP_777353.1, NM_174864.3 [Q8CGU6-1] XP_008767955.1, XM_008769733.1 [Q8CGU6-1] |
3D structure databases
SMRi | Q8CGU6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 252837, 1 interactor |
CORUMi | Q8CGU6 |
IntActi | Q8CGU6, 4 interactors |
STRINGi | 10116.ENSRNOP00000008150 |
PTM databases
GlyGeni | Q8CGU6, 16 sites, 7 N-linked glycans (1 site) |
iPTMneti | Q8CGU6 |
PhosphoSitePlusi | Q8CGU6 |
Proteomic databases
jPOSTi | Q8CGU6 |
PaxDbi | Q8CGU6 |
PRIDEi | Q8CGU6 |
Genome annotation databases
Ensembli | ENSRNOT00000008150; ENSRNOP00000008150; ENSRNOG00000005355 [Q8CGU6-1] |
GeneIDi | 289231 |
KEGGi | rno:289231 |
UCSCi | RGD:631418, rat [Q8CGU6-1] |
Organism-specific databases
CTDi | 23385 |
RGDi | 631418, Ncstn |
Phylogenomic databases
eggNOGi | KOG2657, Eukaryota |
GeneTreei | ENSGT00390000014633 |
HOGENOMi | CLU_024257_0_0_1 |
InParanoidi | Q8CGU6 |
OMAi | SYGSEFA |
OrthoDBi | 777987at2759 |
PhylomeDBi | Q8CGU6 |
TreeFami | TF317086 |
Enzyme and pathway databases
Reactomei | R-RNO-1251985, Nuclear signaling by ERBB4 R-RNO-193692, Regulated proteolysis of p75NTR R-RNO-205043, NRIF signals cell death from the nucleus R-RNO-3928665, EPH-ephrin mediated repulsion of cells R-RNO-6798695, Neutrophil degranulation R-RNO-9013507, NOTCH3 Activation and Transmission of Signal to the Nucleus R-RNO-9017802, Noncanonical activation of NOTCH3 |
Miscellaneous databases
PROi | PR:Q8CGU6 |
Gene expression databases
Bgeei | ENSRNOG00000005355, Expressed in jejunum and 20 other tissues |
ExpressionAtlasi | Q8CGU6, baseline and differential |
Genevisiblei | Q8CGU6, RN |
Family and domain databases
InterProi | View protein in InterPro IPR041084, Ncstrn_small IPR008710, Nicastrin |
PANTHERi | PTHR21092, PTHR21092, 1 hit |
Pfami | View protein in Pfam PF18266, Ncstrn_small, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NICA_RAT | |
Accessioni | Q8CGU6Primary (citable) accession number: Q8CGU6 Secondary accession number(s): Q8CH12 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2003 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 10, 2021 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families