Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2

Gene

Agap2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. Aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase.4 Publications

Activity regulationi

GAP activity is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and, to a lesser extent, by phosphatidylinositol 3,4,5-trisphosphate (PIP3). Phosphatidic acid potentiates PIP2 stimulation (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi407 – 414GTPSequence analysis8
Nucleotide bindingi451 – 455GTPSequence analysis5
Nucleotide bindingi509 – 512GTPSequence analysis4
Zinc fingeri940 – 963C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation
LigandGTP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
Short name:
AGAP-2
Alternative name(s):
Centaurin-gamma-1
Short name:
Cnt-g1
Phosphatidylinositol 3-kinase enhancer
Short name:
PIKE
Gene namesi
Name:Agap2
Synonyms:Centg1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi628844 Agap2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi187P → L: Abolishes interactions with HOMER1C and NF2. 2 Publications1
Mutagenesisi414S → N: Fails to activate PI3 kinase. 1 Publication1
Mutagenesisi679K → N: Abolishes phospholipid binding and nuclear localization; when associated with N-687. 1 Publication1
Mutagenesisi687K → N: Abolishes phospholipid binding and nuclear localization; when associated with N-679. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002359141 – 1186Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2Add BLAST1186

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei113PhosphoserineBy similarity1
Modified residuei128PhosphoserineBy similarity1
Modified residuei149PhosphoserineBy similarity1
Modified residuei632PhosphoserineBy similarity1
Modified residuei744PhosphoserineBy similarity1
Modified residuei746PhosphoserineBy similarity1
Modified residuei802PhosphoserineBy similarity1
Modified residuei921PhosphoserineBy similarity1
Modified residuei979PhosphoserineBy similarity1
Modified residuei1172PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8CGU4
PRIDEiQ8CGU4

PTM databases

iPTMnetiQ8CGU4
PhosphoSitePlusiQ8CGU4

Expressioni

Tissue specificityi

Present in cortex, hippocampus and olfactory bulb but absent in cerebellum (at protein level).2 Publications

Gene expression databases

BgeeiENSRNOG00000025584 Expressed in 10 organ(s), highest expression level in brain
ExpressionAtlasiQ8CGU4 baseline and differential
GenevisibleiQ8CGU4 RN

Interactioni

Subunit structurei

Interacts with EPB41L1, PLCG1, NF2, HOMER1 and HOMER2.4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249323, 3 interactors
IntActiQ8CGU4, 9 interactors
MINTiQ8CGU4
STRINGi10116.ENSRNOP00000029533

Structurei

3D structure databases

ProteinModelPortaliQ8CGU4
SMRiQ8CGU4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini670 – 904PHPROSITE-ProRule annotationAdd BLAST235
Domaini925 – 1045Arf-GAPPROSITE-ProRule annotationAdd BLAST121
Repeati1084 – 1113ANK 1Add BLAST30
Repeati1117 – 1146ANK 2Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 23Interaction with EPB41L11 PublicationAdd BLAST23
Regioni180 – 225Interactions with HOMER1 and NF2Add BLAST46
Regioni262 – 384Interaction with PLCG11 PublicationAdd BLAST123
Regioni399 – 566G domainBy similarityAdd BLAST168

Domaini

G domain binds GTP and has GTPase activity.By similarity
Arf-GAP domain interacts with G domain and may regulate its GTPase activity.By similarity
PH domain binds phospholipids and is required for nuclear targeting.By similarity

Sequence similaritiesi

Belongs to the centaurin gamma-like family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri940 – 963C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0705 Eukaryota
COG5347 LUCA
GeneTreeiENSGT00760000118874
HOGENOMiHOG000007233
HOVERGENiHBG054045
InParanoidiQ8CGU4
KOiK17848
OMAiPAKRKMW
OrthoDBiEOG091G123A
PhylomeDBiQ8CGU4
TreeFamiTF317762

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
2.30.29.30, 2 hits
3.30.40.160, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR037278 ARFGAP/RecO
IPR001164 ArfGAP_dom
IPR038508 ArfGAP_dom_sf
IPR027417 P-loop_NTPase
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF01412 ArfGap, 1 hit
PF00071 Ras, 1 hit
PRINTSiPR00405 REVINTRACTNG
SMARTiView protein in SMART
SM00105 ArfGap, 1 hit
SM00233 PH, 1 hit
SUPFAMiSSF48403 SSF48403, 1 hit
SSF52540 SSF52540, 1 hit
SSF57863 SSF57863, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 1 hit
PS50115 ARFGAP, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS51419 RAB, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8CGU4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSRGAGALQR RTTTYLISLT LVKLESVPPP PPSPSAAAVG APGARGSEPR
60 70 80 90 100
DPGSPRGAEE PGKKRHERLF HRQDALWIST SSAGAGGAEP PALSPAPASP
110 120 130 140 150
ARPVSPAPGR RLSLWAAPPG PPLSGGLSPD SKPGGAPSSS RRPLLSSPSW
160 170 180 190 200
GGPEPEGRTG GGVPGSSSPH PGTGSRRLKV APPPPAPKPF KTVTTSGAKA
210 220 230 240 250
GGGKGAGSRL SWPESEGKPR VKGSKSTAGT GASAVAAGGG GSAAVTTSGG
260 270 280 290 300
VGAGAGARGK LSPRKGKSKT LDNSDLHPGP SAGSPPLTVP AIPVPATSVT
310 320 330 340 350
AASTQPLGPA PPITLEPPAP GLKRGREGGR ASTRDRKMLK FISGIFTKST
360 370 380 390 400
GGPPGPGPLP GPQGLSSSSG SRELLGAELR ASPKAVVNSQ EWTLSRSIPE
410 420 430 440 450
LRLGVLGDVR SGKSSLIHRF LTGSYQVLEK PESEQYKKEM LVDGQTHLVL
460 470 480 490 500
IREEAGAPDA KFSGWADAVI FVFSLEDESS FQAVSHLHGQ LISLRGEGRG
510 520 530 540 550
GLALALVGTQ DRISASSPRV VGDARARALC TDMKRCSYYE TCATYGLNVD
560 570 580 590 600
RVFQEVAQKV VTLRKQQQLL AACKSLPSSP SHSAASTPVA GQASNGGHTS
610 620 630 640 650
DYSSSLPSSP NVGHRELRAE AAAVAGLSTP GSLHRAAKRR TSLFANRRGS
660 670 680 690 700
DSEKRSLDSR GETTGSGRAI PIKQSFLLKR SGNSLNKEWK KKYVTLSSNG
710 720 730 740 750
FLLYHPSIND YIHSTHGKEM DLLRTTVKVP GKRPPRAISA FGPSASINGL
760 770 780 790 800
VKDMSTVQMG EGPEASTPMP SPSPSPSSLQ LPTDQTSKHL LKPDRNLARA
810 820 830 840 850
LSTDCTPSGD LSPLSREPPP SPMVKKQRRK KLSTPSKTEG SAVQAEAKRK
860 870 880 890 900
MWKLKSFGSL RNIYKAEENF EFLIVSSTGQ TWHFEAASFE ERDAWVQAIE
910 920 930 940 950
SQILASLQCC ESSKVKLRTD SQSEAVAIQA IRNAKGNSTC VDCGAPNPTW
960 970 980 990 1000
ASLNLGALIC IECSGIHRNL GTHLSRVRSL DLDDWPRELT LVLTAIGNDT
1010 1020 1030 1040 1050
ANRVWESDTR GRAKPTRDSS REERESWIRA KYEQLLFLAP LGTTEEPLGR
1060 1070 1080 1090 1100
QLWAAVEAQD VAAVLLLLAH ARHGPLDTSV EDPQLRSPLH LAAELAHVVI
1110 1120 1130 1140 1150
TQLLLWYGAD VAARDAQGRT ALFYARQAGS QLCADILLQH GCPGEGGSTA
1160 1170 1180
TTPSAATTPS ITATPSPRRR SSAASLGRVD TTIALV
Length:1,186
Mass (Da):124,438
Last modified:March 1, 2003 - v1
Checksum:iB9C8E987D2989378
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JVS4A0A0G2JVS4_RAT
Arf-GAP with GTPase, ANK repeat and...
Agap2
753Annotation score:

Sequence cautioni

The sequence AAF97595 differs from that shown. Reason: Frameshift at several positions. This sequence would represent a shorter strictly nuclear isoform named PIKE-S. It may be an artifactual frameshifted form whose existence in vivo is dubious.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY128688 mRNA Translation: AAM97539.1
AF280816 mRNA Translation: AAF97595.1 Frameshift.
RefSeqiNP_075415.2, NM_023026.2
UniGeneiRn.91094

Genome annotation databases

EnsembliENSRNOT00000031230; ENSRNOP00000029533; ENSRNOG00000025584
GeneIDi65218
KEGGirno:65218

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY128688 mRNA Translation: AAM97539.1
AF280816 mRNA Translation: AAF97595.1 Frameshift.
RefSeqiNP_075415.2, NM_023026.2
UniGeneiRn.91094

3D structure databases

ProteinModelPortaliQ8CGU4
SMRiQ8CGU4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249323, 3 interactors
IntActiQ8CGU4, 9 interactors
MINTiQ8CGU4
STRINGi10116.ENSRNOP00000029533

PTM databases

iPTMnetiQ8CGU4
PhosphoSitePlusiQ8CGU4

Proteomic databases

PaxDbiQ8CGU4
PRIDEiQ8CGU4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000031230; ENSRNOP00000029533; ENSRNOG00000025584
GeneIDi65218
KEGGirno:65218

Organism-specific databases

CTDi116986
RGDi628844 Agap2

Phylogenomic databases

eggNOGiKOG0705 Eukaryota
COG5347 LUCA
GeneTreeiENSGT00760000118874
HOGENOMiHOG000007233
HOVERGENiHBG054045
InParanoidiQ8CGU4
KOiK17848
OMAiPAKRKMW
OrthoDBiEOG091G123A
PhylomeDBiQ8CGU4
TreeFamiTF317762

Miscellaneous databases

PROiPR:Q8CGU4

Gene expression databases

BgeeiENSRNOG00000025584 Expressed in 10 organ(s), highest expression level in brain
ExpressionAtlasiQ8CGU4 baseline and differential
GenevisibleiQ8CGU4 RN

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
2.30.29.30, 2 hits
3.30.40.160, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR037278 ARFGAP/RecO
IPR001164 ArfGAP_dom
IPR038508 ArfGAP_dom_sf
IPR027417 P-loop_NTPase
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF01412 ArfGap, 1 hit
PF00071 Ras, 1 hit
PRINTSiPR00405 REVINTRACTNG
SMARTiView protein in SMART
SM00105 ArfGap, 1 hit
SM00233 PH, 1 hit
SUPFAMiSSF48403 SSF48403, 1 hit
SSF52540 SSF52540, 1 hit
SSF57863 SSF57863, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 1 hit
PS50115 ARFGAP, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS51419 RAB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAGAP2_RAT
AccessioniPrimary (citable) accession number: Q8CGU4
Secondary accession number(s): Q9JHW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again