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Protein

Calcium-binding and coiled-coil domain-containing protein 1

Gene

Calcoco1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N-terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1-mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions. In association with CCAR1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding and coiled-coil domain-containing protein 1
Alternative name(s):
Coiled-coil coactivator protein
Gene namesi
Name:Calcoco1
Synonyms:CocoA, Kiaa1536
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1914738 Calcoco1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi17F → A: Loss of interaction with p300 KIX domain; eliminated the autonomous transactivation function. 1 Publication1
Mutagenesisi20V → A: Reduced binding to p300 KIX domain; severe reduction in the autonomous transactivation function. 1 Publication1
Mutagenesisi20V → P: Reduced binding to p300 KIX domain; severe reduction in the autonomous transactivation function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003089001 – 691Calcium-binding and coiled-coil domain-containing protein 1Add BLAST691

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8CGU1
PaxDbiQ8CGU1
PRIDEiQ8CGU1

PTM databases

iPTMnetiQ8CGU1
PhosphoSitePlusiQ8CGU1

Expressioni

Tissue specificityi

Expressed in all tissues examined except spleen, with high levels of expression in the heart and kidney.2 Publications

Gene expression databases

BgeeiENSMUSG00000023055 Expressed in 220 organ(s), highest expression level in ascending aorta
CleanExiMM_CALCOCO1
ExpressionAtlasiQ8CGU1 baseline and differential
GenevisibleiQ8CGU1 MM

Interactioni

Subunit structurei

Part of a calphoglin complex consisting of CALCOCO1, PPA1 and PGM (By similarity). Interacts with the bHLH-PAS domains of GRIP1, AHR and ARNT. Interacts with CTNNB1 via both its N- and C-terminal regions. Interacts with EP300. Interacts with CCAR1 (via N-terminus) and GATA1.By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BcatO424864EBI-972374,EBI-972394From Gallus gallus.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212224, 3 interactors
IntActiQ8CGU1, 2 interactors
STRINGi10090.ENSMUSP00000023818

Structurei

3D structure databases

ProteinModelPortaliQ8CGU1
SMRiQ8CGU1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 190N-terminal AD (CTNNB1 binding site)Add BLAST190
Regioni1 – 30p300 KIX-bindingAdd BLAST30
Regioni45 – 125Interaction with GATA11 PublicationAdd BLAST81
Regioni501 – 691C-terminal AD (CTNNB1 binding site); interaction with CCAR11 PublicationAdd BLAST191

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili145 – 205Sequence analysisAdd BLAST61
Coiled coili232 – 339Sequence analysisAdd BLAST108
Coiled coili417 – 514Sequence analysisAdd BLAST98

Domaini

The C-terminal activation region (AD) is used for downstream signaling. Seems to be essential for coactivator function with nuclear receptors and with the aryl hydrocarbon receptor.
The N-terminal activation region (AD) is necessary and sufficient for synergistic activation of LEF1-mediated transcription by CTNNB1. Contains a EP3000 binding region which is important for synergistic cooperation.
Recruitment by nuclear receptors is accomplished by the interaction of the coiled-coiled domain with p160 coactivators.

Sequence similaritiesi

Belongs to the CALCOCO family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IHKH Eukaryota
ENOG410ZVZ6 LUCA
GeneTreeiENSGT00530000063216
HOGENOMiHOG000111283
HOVERGENiHBG107573
InParanoidiQ8CGU1
OMAiYDMASGF
OrthoDBiEOG091G01W2
PhylomeDBiQ8CGU1
TreeFamiTF329501

Family and domain databases

InterProiView protein in InterPro
IPR012852 CALCOCO1-like
PfamiView protein in Pfam
PF07888 CALCOCO1, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.iShow all

Q8CGU1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEESSLSRAP SRGGVNFLNV ARTYIPNTKV ECHYTLPPGT MPSASDWIGI
60 70 80 90 100
FKVEAACVRD YHTFVWSSVP ESTTDGSPTH ASVQFQASYL PKPGAQLYQF
110 120 130 140 150
RYVNRQGRVC GQSPPFQFRE PRPMDELVTL EEADGGSDIL LVVPKATVLQ
160 170 180 190 200
NQLDESQQER NDLMQLKLQL EDQVTELRSR VQELEAALAT ARQEHSELTE
210 220 230 240 250
QYKGLSRSHG ELSEERDILS QQQGDHVARI LELEDDIQTM SDKVLMKEVE
260 270 280 290 300
LDRVRDTVKA LTREQEKLLR QLKEFQADKE QSEAELQTVR EENCCLNTEL
310 320 330 340 350
EEAKSRQEEQ GAQVQRLKDK LAHMKDTLGQ AQQKVAELEP LKEQLRGVQE
360 370 380 390 400
LAASSQQKAA LLGEELASAA GARDRTIAEL HRSRLEVAEV NGRLAELSLH
410 420 430 440 450
MKEEKCQWSK ERTGLLQSME AEKDKILKLS AEILRLEKTV QEERTQSHVF
460 470 480 490 500
KTELAREKDS SLVQLSESKR ELTELRSALR VLQKEKEQLQ TEKQELLEYM
510 520 530 540 550
RKLEARLEKV ADEKWTEDAA TEDEEATAGL SCPASLTDSE DESPEDMRLP
560 570 580 590 600
SYGLCESGNT SSSPPGPREP SSLVVINQPA PIAPQFSGPG EASSSDSEAE
610 620 630 640 650
DEKSVLMAAV QSGGEEASLL LPELGSAFYD VASAFTVSSL SEASPGVPAN
660 670 680 690
PPWKECPICK ERFPAESDKD ALEGHMDGHF FFSTQDPFTF E
Length:691
Mass (Da):77,280
Last modified:October 23, 2007 - v2
Checksum:i4759E5478839D9B7
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q7U2E9Q7U2_MOUSE
Calcium-binding and coiled-coil dom...
Calcoco1
633Annotation score:
F6Q5V4F6Q5V4_MOUSE
Calcium-binding and coiled-coil dom...
Calcoco1
232Annotation score:
A0A2R8VKP5A0A2R8VKP5_MOUSE
Calcium-binding and coiled-coil dom...
Calcoco1
606Annotation score:
A0A2R8VHU8A0A2R8VHU8_MOUSE
Calcium-binding and coiled-coil dom...
Calcoco1
533Annotation score:
A0A2R8VKE2A0A2R8VKE2_MOUSE
Calcium-binding and coiled-coil dom...
Calcoco1
68Annotation score:

Sequence cautioni

The sequence BAD90452 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti301E → Q in AAN10148 (PubMed:14690606).Curated1
Sequence conflicti301E → Q in BAD90452 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY131329 mRNA Translation: AAN10148.1
AK007393 mRNA Translation: BAB25009.1
AK035598 mRNA Translation: BAC29119.1
AK220400 mRNA Translation: BAD90452.1 Different initiation.
BC054783 mRNA Translation: AAH54783.1
CCDSiCCDS27889.1
RefSeqiNP_080468.1, NM_026192.3
UniGeneiMm.71682

Genome annotation databases

EnsembliENSMUST00000023818; ENSMUSP00000023818; ENSMUSG00000023055
GeneIDi67488
KEGGimmu:67488
UCSCiuc007xws.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCACO1_MOUSE
AccessioniPrimary (citable) accession number: Q8CGU1
Secondary accession number(s): Q5DTX0, Q9D935
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: September 12, 2018
This is version 106 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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