UniProtKB - Q8CGF1 (RHG29_MOUSE)
Rho GTPase-activating protein 29
Arhgap29
Functioni
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho (By similarity).
In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis.
By similarity1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 614 – 659 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 46 |
GO - Molecular functioni
- GTPase activator activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- PDZ domain binding Source: MGI
GO - Biological processi
- activation of GTPase activity Source: GO_Central
- intracellular signal transduction Source: InterPro
Keywordsi
Molecular function | GTPase activation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-8980692, RHOA GTPase cycle R-MMU-9013148, CDC42 GTPase cycle R-MMU-9013149, RAC1 GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Rho GTPase-activating protein 29Alternative name(s): Rho-type GTPase-activating protein 29 |
Gene namesi | Name:Arhgap29 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2443818, Arhgap29 |
VEuPathDBi | HostDB:ENSMUSG00000039831 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000317583 | 1 – 1266 | Rho GTPase-activating protein 29Add BLAST | 1266 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 171 | PhosphoserineCombined sources | 1 | |
Modified residuei | 176 | PhosphoserineCombined sources | 1 | |
Modified residuei | 179 | PhosphoserineCombined sources | 1 | |
Modified residuei | 190 | PhosphoserineCombined sources | 1 | |
Modified residuei | 501 | PhosphoserineBy similarity | 1 | |
Modified residuei | 521 | PhosphoserineCombined sources | 1 | |
Modified residuei | 554 | PhosphoserineBy similarity | 1 | |
Modified residuei | 920 | PhosphoserineBy similarity | 1 | |
Modified residuei | 956 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1028 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1149 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1151 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q8CGF1 |
MaxQBi | Q8CGF1 |
PaxDbi | Q8CGF1 |
PeptideAtlasi | Q8CGF1 |
PRIDEi | Q8CGF1 |
ProteomicsDBi | 255332 [Q8CGF1-1] 255333 [Q8CGF1-2] |
PTM databases
iPTMneti | Q8CGF1 |
PhosphoSitePlusi | Q8CGF1 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000039831, Expressed in cumulus cell and 269 other tissues |
Genevisiblei | Q8CGF1, MM |
Interactioni
Subunit structurei
Interacts with PTPN13/PTPL1.
Interacts with RAP2A via its coiled coil domain (By similarity).
Interacts with RASIP1.
By similarity1 PublicationGO - Molecular functioni
- PDZ domain binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 229497, 9 interactors |
STRINGi | 10090.ENSMUSP00000044624 |
Miscellaneous databases
RNActi | Q8CGF1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 192 – 462 | F-BARPROSITE-ProRule annotationAdd BLAST | 271 | |
Domaini | 673 – 888 | Rho-GAPPROSITE-ProRule annotationAdd BLAST | 216 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 482 – 501 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 542 – 601 | DisorderedSequence analysisAdd BLAST | 60 | |
Regioni | 1039 – 1081 | DisorderedSequence analysisAdd BLAST | 43 | |
Regioni | 1116 – 1157 | DisorderedSequence analysisAdd BLAST | 42 | |
Regioni | 1209 – 1266 | DisorderedSequence analysisAdd BLAST | 58 | |
Regioni | 1263 – 1266 | Interaction with PTPN13/PTPL1By similarity | 4 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 296 – 418 | Sequence analysisAdd BLAST | 123 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 482 – 497 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 542 – 559 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 1048 – 1074 | Basic and acidic residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 1128 – 1145 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 1217 – 1231 | Polar residuesSequence analysisAdd BLAST | 15 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 614 – 659 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 46 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1453, Eukaryota |
GeneTreei | ENSGT00950000183110 |
HOGENOMi | CLU_006236_2_0_1 |
InParanoidi | Q8CGF1 |
OMAi | REHCKAC |
OrthoDBi | 1300981at2759 |
PhylomeDBi | Q8CGF1 |
TreeFami | TF351450 |
Family and domain databases
CDDi | cd00029, C1, 1 hit |
Gene3Di | 1.10.555.10, 1 hit 1.20.1270.60, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR031160, F_BAR IPR002219, PE/DAG-bd IPR008936, Rho_GTPase_activation_prot IPR000198, RhoGAP_dom |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF00620, RhoGAP, 1 hit |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00324, RhoGAP, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF48350, SSF48350, 1 hit |
PROSITEi | View protein in PROSITE PS51741, F_BAR, 1 hit PS50238, RHOGAP, 1 hit PS00479, ZF_DAG_PE_1, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MIAHKQKKAK KKRVWASGQP SAAITTSEMG LKSVSSSSSF DPEYIKELVN
60 70 80 90 100
DVRKFSHMLL YLKEAILSDC FKEVIHIRLD ELLRVLKSIL SKHQNLSSVD
110 120 130 140 150
LQSAAEVLTA KVKAVNFTEV NEENKNDIFR EVFSSIETLA FTFGNILTNF
160 170 180 190 200
LMGDVGSDSI LRLPISRESK SFENISVDSV DLPHEKGNFS PIELDNLLLK
210 220 230 240 250
NTDSIELALS YAKTWSKYTK NIVSWVEKKL NLELESTRNI VKLAEATRSS
260 270 280 290 300
IGIQEFMPLQ SLFTNALLSD IHSSHLLQQT IAALQANKFV QPLLGRKNEM
310 320 330 340 350
EKQRKEIKDL WKQQQNKLLE TETALKKAKL LCMQRQDEYE KAKSSMFRAE
360 370 380 390 400
EEQLSSSVGL AKNLNKQLEK RRRLEEEALQ KVEEANEHYK VCVTNVEERR
410 420 430 440 450
NDLENTKREI LTQLRTLVFQ CDLTLKAVTV NLFHMQQLQA ASLANSLQSL
460 470 480 490 500
CDSAKLYDPG QEYSEFVKAT SSSELEEKVD GNVNKQMTNS PQTSGYEPAD
510 520 530 540 550
SLEDVARLPD SCHKLEEDRC SNSADMTGPS FVRSWKFGMF SDSESTGGSS
560 570 580 590 600
ESRSLDSESI SPGDFHRKLP RTPSSGTMSS ADDLDEREPP SPSEAGPNSL
610 620 630 640 650
GAFKKTLMSK AALTHKFRKL RSPTKCRDCD GIVMFPGVEC EECLLVCHRK
660 670 680 690 700
CLENLVIICG HQKLQGKMHI FGAEFIQVAK KEPDGIPFVL KICASEIENR
710 720 730 740 750
ALCLQGIYRV CGNKIKTEKL CQALENGMHL VDISEFSSHD ICDVLKLYLR
760 770 780 790 800
QLPEPFILFR LYKEFIDLAK EIQHVNEEQE AKKDSPEDKK HPHVSIEVNR
810 820 830 840 850
ILLKSKDLLR QLPASHFNSL HYLIAHLRRV VDHAEENKMN SKNLGVIFGP
860 870 880 890 900
TLIRPRPTTA PVTISSLAEY SNQARLVEFL ITYSQKIFDG SLQPQAVVIS
910 920 930 940 950
NTGAVAPQVD QGYLPKPLLS PDERDTDHSM KPLFFSSKED IRSSDCESKS
960 970 980 990 1000
FELTTSFEES ERRQNALGKC DAPLLDNKVH LLFDQEHESA SQKMEDVCKS
1010 1020 1030 1040 1050
PKLLLLKSNR AANSVQRHTP RTKMRPVSLP VDRLLLLASS PTERSSRDVG
1060 1070 1080 1090 1100
NVDSDKFGKN PAFEGLHRKD NSNTTRSKVN GFDQQNVQKS WDTQYVRNNF
1110 1120 1130 1140 1150
TAKTTMIVPS AYPEKGLTVN TGNNRDHPGS KAHAEPARAA GDVSERRSSD
1160 1170 1180 1190 1200
SCPATAVRAP RTLQPQHWTT FYKPPNPTFS VRGTEEKTAL PSIAVPPVLV
1210 1220 1230 1240 1250
HAPQIHVTKS DPDSEATLAC PVQTSGQPKE SSEEPALPEG TPTCQRPRLK
1260
RMQQFEDLED EIPQFV
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JEY0 | A0A0G2JEY0_MOUSE | Rho GTPase-activating protein 29 | Arhgap29 | 780 | Annotation score: | ||
A0A0G2JGF7 | A0A0G2JGF7_MOUSE | Rho GTPase-activating protein 29 | Arhgap29 | 859 | Annotation score: | ||
A0A0G2JEL5 | A0A0G2JEL5_MOUSE | Rho GTPase-activating protein 29 | Arhgap29 | 272 | Annotation score: | ||
A0A0G2JGF8 | A0A0G2JGF8_MOUSE | Rho GTPase-activating protein 29 | Arhgap29 | 83 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 165 | I → T in AAH52858 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 201 | N → D in BAE21318 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 224 | S → L in BAE21318 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 338 | E → G in BAC31603 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 1088 | Q → R in AAH52858 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_031060 | 379 – 1035 | Missing in isoform 2. 1 PublicationAdd BLAST | 657 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK043637 mRNA Translation: BAC31603.2 AK044979 mRNA Translation: BAC32168.2 AK132716 mRNA Translation: BAE21318.1 BC040387 mRNA Translation: AAH40387.1 BC052858 mRNA Translation: AAH52858.1 |
CCDSi | CCDS17807.1 [Q8CGF1-1] |
RefSeqi | NP_766113.1, NM_172525.2 [Q8CGF1-1] XP_006501351.1, XM_006501288.3 |
Genome annotation databases
Ensembli | ENSMUST00000037958; ENSMUSP00000044624; ENSMUSG00000039831 [Q8CGF1-1] |
GeneIDi | 214137 |
KEGGi | mmu:214137 |
UCSCi | uc008reh.1, mouse [Q8CGF1-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK043637 mRNA Translation: BAC31603.2 AK044979 mRNA Translation: BAC32168.2 AK132716 mRNA Translation: BAE21318.1 BC040387 mRNA Translation: AAH40387.1 BC052858 mRNA Translation: AAH52858.1 |
CCDSi | CCDS17807.1 [Q8CGF1-1] |
RefSeqi | NP_766113.1, NM_172525.2 [Q8CGF1-1] XP_006501351.1, XM_006501288.3 |
3D structure databases
SMRi | Q8CGF1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 229497, 9 interactors |
STRINGi | 10090.ENSMUSP00000044624 |
PTM databases
iPTMneti | Q8CGF1 |
PhosphoSitePlusi | Q8CGF1 |
Proteomic databases
jPOSTi | Q8CGF1 |
MaxQBi | Q8CGF1 |
PaxDbi | Q8CGF1 |
PeptideAtlasi | Q8CGF1 |
PRIDEi | Q8CGF1 |
ProteomicsDBi | 255332 [Q8CGF1-1] 255333 [Q8CGF1-2] |
Protocols and materials databases
Antibodypediai | 19948, 98 antibodies from 18 providers |
DNASUi | 214137 |
Genome annotation databases
Ensembli | ENSMUST00000037958; ENSMUSP00000044624; ENSMUSG00000039831 [Q8CGF1-1] |
GeneIDi | 214137 |
KEGGi | mmu:214137 |
UCSCi | uc008reh.1, mouse [Q8CGF1-1] |
Organism-specific databases
CTDi | 9411 |
MGIi | MGI:2443818, Arhgap29 |
VEuPathDBi | HostDB:ENSMUSG00000039831 |
Phylogenomic databases
eggNOGi | KOG1453, Eukaryota |
GeneTreei | ENSGT00950000183110 |
HOGENOMi | CLU_006236_2_0_1 |
InParanoidi | Q8CGF1 |
OMAi | REHCKAC |
OrthoDBi | 1300981at2759 |
PhylomeDBi | Q8CGF1 |
TreeFami | TF351450 |
Enzyme and pathway databases
Reactomei | R-MMU-8980692, RHOA GTPase cycle R-MMU-9013148, CDC42 GTPase cycle R-MMU-9013149, RAC1 GTPase cycle |
Miscellaneous databases
BioGRID-ORCSi | 214137, 0 hits in 64 CRISPR screens |
ChiTaRSi | Arhgap29, mouse |
PROi | PR:Q8CGF1 |
RNActi | Q8CGF1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039831, Expressed in cumulus cell and 269 other tissues |
Genevisiblei | Q8CGF1, MM |
Family and domain databases
CDDi | cd00029, C1, 1 hit |
Gene3Di | 1.10.555.10, 1 hit 1.20.1270.60, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR031160, F_BAR IPR002219, PE/DAG-bd IPR008936, Rho_GTPase_activation_prot IPR000198, RhoGAP_dom |
Pfami | View protein in Pfam PF00130, C1_1, 1 hit PF00620, RhoGAP, 1 hit |
SMARTi | View protein in SMART SM00109, C1, 1 hit SM00324, RhoGAP, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF48350, SSF48350, 1 hit |
PROSITEi | View protein in PROSITE PS51741, F_BAR, 1 hit PS50238, RHOGAP, 1 hit PS00479, ZF_DAG_PE_1, 1 hit PS50081, ZF_DAG_PE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RHG29_MOUSE | |
Accessioni | Q8CGF1Primary (citable) accession number: Q8CGF1 Secondary accession number(s): Q3V135 Q8BLR8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 5, 2008 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot