UniProtKB - Q8CGE9 (RGS12_MOUSE)
Protein
Regulator of G-protein signaling 12
Gene
Rgs12
Organism
Mus musculus (Mouse)
Status
Functioni
Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form.By similarity
GO - Molecular functioni
- G-protein alpha-subunit binding Source: MGI
- GTPase activator activity Source: MGI
- GTPase regulator activity Source: MGI
GO - Biological processi
- signal transduction Source: InterPro
- termination of G protein-coupled receptor signaling pathway Source: MGI
Keywordsi
Molecular function | GTPase activation |
Enzyme and pathway databases
Reactomei | R-MMU-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Regulator of G-protein signaling 12Short name: RGS12 |
Gene namesi | Name:Rgs12 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1918979, Rgs12 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- nucleolus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- apical dendrite Source: MGI
- cytoplasm Source: UniProtKB
- dendrite Source: UniProtKB
- neuronal cell body Source: MGI
- protein-containing complex Source: MGI
- synapse Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Cell projection, Cytoplasm, Nucleus, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000408474 | 1 – 1381 | Regulator of G-protein signaling 12Add BLAST | 1381 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 171 | PhosphoserineCombined sources | 1 | |
Modified residuei | 194 | PhosphoserineCombined sources | 1 | |
Cross-linki | 195 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 524 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 633 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 661 | PhosphoserineCombined sources | 1 | |
Modified residuei | 671 | PhosphoserineCombined sources | 1 | |
Modified residuei | 850 | PhosphoserineCombined sources | 1 | |
Modified residuei | 879 | PhosphoserineCombined sources | 1 | |
Modified residuei | 943 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8CGE9 |
jPOSTi | Q8CGE9 |
MaxQBi | Q8CGE9 |
PaxDbi | Q8CGE9 |
PRIDEi | Q8CGE9 |
PTM databases
iPTMneti | Q8CGE9 |
PhosphoSitePlusi | Q8CGE9 |
Expressioni
Tissue specificityi
Expressed in brain.1 Publication
Developmental stagei
Expressed in germinal vesicle oocyte, metaphase II oocyte and blastocyst (at protein level). Expressed in oocyte.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000029101, Expressed in ear and 224 other tissues |
Genevisiblei | Q8CGE9, MM |
Interactioni
Subunit structurei
Interacts with GNAI1, GNAI2 and GNAI3; the interactions are GDP-dependent.
By similarityBinary interactionsi
Hide detailsQ8CGE9
With | #Exp. | IntAct |
---|---|---|
Map2k2 [Q63932] | 3 | EBI-7340552,EBI-397724 |
BRAF [P15056] from Homo sapiens. | 2 | EBI-7340552,EBI-365980 |
GO - Molecular functioni
- G-protein alpha-subunit binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 214886, 2 interactors |
IntActi | Q8CGE9, 7 interactors |
MINTi | Q8CGE9 |
STRINGi | 10090.ENSMUSP00000030984 |
Miscellaneous databases
RNActi | Q8CGE9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 98 | PDZPROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 223 – 390 | PIDPROSITE-ProRule annotationAdd BLAST | 168 | |
Domaini | 715 – 832 | RGSPROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 962 – 1032 | RBD 1PROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 1034 – 1104 | RBD 2PROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 1187 – 1209 | GoLocoPROSITE-ProRule annotationAdd BLAST | 23 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1362 – 1367 | Poly-Pro | 6 |
Domaini
The GoLoco domain is necessary for interaction with GNAI1, GNAI2 and GNAI3.By similarity
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3589, Eukaryota |
GeneTreei | ENSGT00940000159741 |
HOGENOMi | CLU_002190_0_0_1 |
InParanoidi | Q8CGE9 |
TreeFami | TF328814 |
Family and domain databases
CDDi | cd08742, RGS_RGS12, 1 hit |
Gene3Di | 1.10.196.10, 1 hit 2.30.29.30, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR003109, GoLoco_motif IPR001478, PDZ IPR036034, PDZ_sf IPR011993, PH-like_dom_sf IPR006020, PTB/PI_dom IPR003116, RBD_dom IPR016137, RGS IPR037880, RGS12_RGS IPR036305, RGS_sf IPR024066, RGS_subdom1/3 IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF02188, GoLoco, 1 hit PF00595, PDZ, 1 hit PF02196, RBD, 2 hits PF00615, RGS, 1 hit |
PRINTSi | PR01301, RGSPROTEIN |
SMARTi | View protein in SMART SM00390, GoLoco, 1 hit SM00228, PDZ, 1 hit SM00462, PTB, 1 hit SM00455, RBD, 2 hits SM00315, RGS, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit SSF50156, SSF50156, 1 hit SSF54236, SSF54236, 2 hits |
PROSITEi | View protein in PROSITE PS50877, GOLOCO, 1 hit PS50106, PDZ, 1 hit PS01179, PID, 1 hit PS50898, RBD, 2 hits PS50132, RGS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 11 potential isoforms that are computationally mapped.Show allAlign All
Q8CGE9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYRAGEPGKR QPGPAPPRVR SVEVARGRAG YGFTLSGQAP CVLSCVMRGS
60 70 80 90 100
PADFVGLRAG DQILAINEIN VKKASHEDVV KLIGKCSGVL HMVIAEGTSH
110 120 130 140 150
VESCSSDEEG GLYEGKGWLR PKLDSKALGI NRAERVVEEV QSGGIFNMIF
160 170 180 190 200
ESSSLCASGP EPLKLKQRSL SESAALRLDA GQAGLCAPHP SMLSKEDISK
210 220 230 240 250
VINDDSVFTV GLDSHDDFGL DASILNVAMV VGYLGSIELP STSSNLEHDS
260 270 280 290 300
LQAIRGCMRR LRAEQKIHSL VTMKVMHDCV QLVTDRAGVV AEYPAEKLAF
310 320 330 340 350
SAVCPDDRRF FGLVTMQTND DGGLAQEDEG ALRTSCHVFM VDPDLFHHKI
360 370 380 390 400
HQGIARRFGF ACTADPDTSG CLEFPASSLP VLQFISVLYR DMGELIEGVR
410 420 430 440 450
ARAFLDGDAD AHQNNSTSSN SDSGIGNFNQ EEKSNRVLVV DLGGGSSRHG
460 470 480 490 500
QGSSPGWESG GGRGSQPWSA PWNGAFCHDS EAGSPLETSP NTDRFWDLTK
510 520 530 540 550
HSGPVSHMEV PPATLRSSIP PSKRGAAGSS CGFNQRWLPV HVLQEWQCGH
560 570 580 590 600
ASDQESYTDS TDGWSSVNCG TLPPPMSKIP ADRYRVEGSF AQAPLSTQKR
610 620 630 640 650
DWSRKAFGMQ NLFGPHRNVR KTKEDKKSSK LGRGVALAQT SQRTSARRSF
660 670 680 690 700
GRSRRFSITR SLDDLESATV SDGELTGADL KDCISNNSLS SNASLPSVQS
710 720 730 740 750
CRRLRERRVA SWAVSFERLL QDPVGVRYFS DFLRKEFSEE NILFWQACEC
760 770 780 790 800
FSHVPAHDKK ELSYRAREIF SKFLCSKATT PVNIDSQAQL ADDILNAPHP
810 820 830 840 850
DMFKEQQLQI FNLMKFDSYT RFLKSQLYQE CVLAEVEGRT LPDSQQVPSS
860 870 880 890 900
PASKHSISSD HSNVSTPKKL SGKSKSGRSL NEDVGEEDSE KKRRGAFFSW
910 920 930 940 950
SRSRSTGRSQ KKKDHGDHAH DAPHANGGLC RRESQGSVSS AGSLDLSEAC
960 970 980 990 1000
RTSALEKDKA AKHCCVHLPD GTSCVVAVKS GFSIKEILSG LCERHGINGA
1010 1020 1030 1040 1050
AVDLFLVGGD KPLVLHQDSS ILATRDLRLE KRTLFRLDLV PINRSVGLKA
1060 1070 1080 1090 1100
KPTKPVTEVL RPVVAKYGLD LGSLLVRLSG EKEPLDLGAP ISSLDGQRVI
1110 1120 1130 1140 1150
LEERDPSRGK VSTDKQKGAP VKQNSAVNSS PRNHLAMGEE RTLGKSNSIK
1160 1170 1180 1190 1200
IRGENGKSAR DPRLSKREES IAKIGKKKYQ KINLDEAEEF FELISKAQSN
1210 1220 1230 1240 1250
RADDQRGLLR KEDLVLPEFL RLPAGSSELA LSSPPPVKGY SKRAVTGHGQ
1260 1270 1280 1290 1300
EGAAQTEESY SDSPATSPAS AQSPCSAYSP GSAHSPGSAH STPGPPGTTQ
1310 1320 1330 1340 1350
PGEKPTKPSC VSMVQEGTTQ AWRRLSPEME AGGIQTVEDE QVADLTLMGE
1360 1370 1380
GDISSPNSTL LPPPPTPQDT PGPPRPGTSR F
Computationally mapped potential isoform sequencesi
There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q1K7 | E9Q1K7_MOUSE | Regulator of G-protein-signaling 12 | Rgs12 | 723 | Annotation score: | ||
E9Q652 | E9Q652_MOUSE | Regulator of G-protein-signaling 12 | Rgs12 | 1,451 | Annotation score: | ||
D3Z0G5 | D3Z0G5_MOUSE | Regulator of G-protein-signaling 12 | Rgs12 | 803 | Annotation score: | ||
D3Z0G6 | D3Z0G6_MOUSE | Regulator of G-protein-signaling 12 | Rgs12 | 733 | Annotation score: | ||
D3Z0G7 | D3Z0G7_MOUSE | Regulator of G-protein-signaling 12 | Rgs12 | 793 | Annotation score: | ||
Q3TTW9 | Q3TTW9_MOUSE | Regulator of G-protein-signaling 12 | Rgs12 | 499 | Annotation score: | ||
F7B2U5 | F7B2U5_MOUSE | Regulator of G-protein-signaling 12 | Rgs12 | 236 | Annotation score: | ||
F6YRR2 | F6YRR2_MOUSE | Regulator of G-protein-signaling 12 | Rgs12 | 107 | Annotation score: | ||
D3YXA5 | D3YXA5_MOUSE | Regulator of G-protein-signaling 12 | Rgs12 | 116 | Annotation score: | ||
A0A0J9YU30 | A0A0J9YU30_MOUSE | Regulator of G-protein-signaling 12 | Rgs12 | 185 | Annotation score: | ||
There is more potential isoformShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 536 | R → K in AAH40396 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 1135 | L → S in AAH40396 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC126447 Genomic DNA No translation available. AC133204 Genomic DNA No translation available. BC040396 mRNA Translation: AAH40396.1 |
CCDSi | CCDS19222.1 |
RefSeqi | NP_775578.2, NM_173402.2 |
Genome annotation databases
Ensembli | ENSMUST00000030984; ENSMUSP00000030984; ENSMUSG00000029101 |
GeneIDi | 71729 |
KEGGi | mmu:71729 |
UCSCi | uc008xdg.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC126447 Genomic DNA No translation available. AC133204 Genomic DNA No translation available. BC040396 mRNA Translation: AAH40396.1 |
CCDSi | CCDS19222.1 |
RefSeqi | NP_775578.2, NM_173402.2 |
3D structure databases
SMRi | Q8CGE9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 214886, 2 interactors |
IntActi | Q8CGE9, 7 interactors |
MINTi | Q8CGE9 |
STRINGi | 10090.ENSMUSP00000030984 |
PTM databases
iPTMneti | Q8CGE9 |
PhosphoSitePlusi | Q8CGE9 |
Proteomic databases
EPDi | Q8CGE9 |
jPOSTi | Q8CGE9 |
MaxQBi | Q8CGE9 |
PaxDbi | Q8CGE9 |
PRIDEi | Q8CGE9 |
Protocols and materials databases
Antibodypediai | 22482, 94 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000030984; ENSMUSP00000030984; ENSMUSG00000029101 |
GeneIDi | 71729 |
KEGGi | mmu:71729 |
UCSCi | uc008xdg.2, mouse |
Organism-specific databases
CTDi | 6002 |
MGIi | MGI:1918979, Rgs12 |
Phylogenomic databases
eggNOGi | KOG3589, Eukaryota |
GeneTreei | ENSGT00940000159741 |
HOGENOMi | CLU_002190_0_0_1 |
InParanoidi | Q8CGE9 |
TreeFami | TF328814 |
Enzyme and pathway databases
Reactomei | R-MMU-418594, G alpha (i) signalling events |
Miscellaneous databases
BioGRID-ORCSi | 71729, 1 hit in 20 CRISPR screens |
ChiTaRSi | Rgs12, mouse |
PROi | PR:Q8CGE9 |
RNActi | Q8CGE9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029101, Expressed in ear and 224 other tissues |
Genevisiblei | Q8CGE9, MM |
Family and domain databases
CDDi | cd08742, RGS_RGS12, 1 hit |
Gene3Di | 1.10.196.10, 1 hit 2.30.29.30, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR003109, GoLoco_motif IPR001478, PDZ IPR036034, PDZ_sf IPR011993, PH-like_dom_sf IPR006020, PTB/PI_dom IPR003116, RBD_dom IPR016137, RGS IPR037880, RGS12_RGS IPR036305, RGS_sf IPR024066, RGS_subdom1/3 IPR029071, Ubiquitin-like_domsf |
Pfami | View protein in Pfam PF02188, GoLoco, 1 hit PF00595, PDZ, 1 hit PF02196, RBD, 2 hits PF00615, RGS, 1 hit |
PRINTSi | PR01301, RGSPROTEIN |
SMARTi | View protein in SMART SM00390, GoLoco, 1 hit SM00228, PDZ, 1 hit SM00462, PTB, 1 hit SM00455, RBD, 2 hits SM00315, RGS, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit SSF50156, SSF50156, 1 hit SSF54236, SSF54236, 2 hits |
PROSITEi | View protein in PROSITE PS50877, GOLOCO, 1 hit PS50106, PDZ, 1 hit PS01179, PID, 1 hit PS50898, RBD, 2 hits PS50132, RGS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RGS12_MOUSE | |
Accessioni | Q8CGE9Primary (citable) accession number: Q8CGE9 Secondary accession number(s): E9PXX2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 31, 2011 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot