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Protein

RNA-binding protein 28

Gene

Rbm28

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Nucleolar component of the spliceosomal ribonucleoprotein complexes.By similarity

GO - Molecular functioni

  • RNA binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein 28
Alternative name(s):
RNA-binding motif protein 28
Gene namesi
Name:Rbm28
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2655711 Rbm28

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000817862 – 750RNA-binding protein 28Add BLAST749

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei122PhosphoserineBy similarity1
Modified residuei231PhosphoserineCombined sources1
Modified residuei239PhosphoserineCombined sources1
Modified residuei387PhosphoserineBy similarity1
Cross-linki643Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8CGC6
MaxQBiQ8CGC6
PaxDbiQ8CGC6
PeptideAtlasiQ8CGC6
PRIDEiQ8CGC6

PTM databases

iPTMnetiQ8CGC6
PhosphoSitePlusiQ8CGC6
SwissPalmiQ8CGC6

Expressioni

Gene expression databases

BgeeiENSMUSG00000029701 Expressed in 277 organ(s), highest expression level in testis
CleanExiMM_RBM28
ExpressionAtlasiQ8CGC6 baseline and differential
GenevisibleiQ8CGC6 MM

Interactioni

Subunit structurei

Interacts with U1, U2, U4, U5, and U6 spliceosomal small nuclear RNAs (snRNAs).By similarity

Protein-protein interaction databases

BioGridi212775, 3 interactors
DIPiDIP-58949N
IntActiQ8CGC6, 1 interactor
STRINGi10090.ENSMUSP00000007993

Structurei

Secondary structure

1750
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8CGC6
SMRiQ8CGC6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8CGC6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 80RRM 1PROSITE-ProRule annotationAdd BLAST77
Domaini114 – 191RRM 2PROSITE-ProRule annotationAdd BLAST78
Domaini325 – 409RRM 3PROSITE-ProRule annotationAdd BLAST85
Domaini477 – 582RRM 4PROSITE-ProRule annotationAdd BLAST106

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi225 – 306Asp/Glu-rich (acidic)Add BLAST82

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0127 Eukaryota
ENOG410XSHP LUCA
GeneTreeiENSGT00550000074976
HOVERGENiHBG073213
InParanoidiQ8CGC6
KOiK14573
OMAiVGCAFVQ
OrthoDBiEOG091G0JKS
TreeFamiTF312798

Family and domain databases

Gene3Di3.30.70.330, 4 hits
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 4 hits
SMARTiView protein in SMART
SM00360 RRM, 4 hits
SUPFAMiSSF54928 SSF54928, 4 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 4 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q8CGC6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGLTLFVGR LPPSARSDQL EELFSQVGPV KQCFVVTEKG SKACRGFGYV
60 70 80 90 100
TFSMLEDVQR ALKEITTFEG CKIDVTVAKK KLRNKSKETR KNENAESPKK
110 120 130 140 150
EPKHKKAKVA DKKARLIIRN LSFKCSEDDL KAVFTHYGTV LEVNIPKKPD
160 170 180 190 200
GKMRGFAFVQ FKNLLEAGKA LKGANMKEIK GRTVAVDWAV AKDKYKDAQH
210 220 230 240 250
ASAPGVKKSS DRKPKESGKK NCRVEEQVED SDDEEDDDSH DDEEERESTI
260 270 280 290 300
ASPVSVHKRA VKRAAPEESI EEDDSYEDSD LEEGGSSYDE GTVDSESSAE
310 320 330 340 350
DQEDEDVPVS EKKKRKLPSD VTEGKTVFIR NLSFDSEEEA LGEVLQQFGD
360 370 380 390 400
LKYVRVVLHP DTEHSKGCAF AQFMTQEAAQ KCLAAASLEA EGGGLKLDGR
410 420 430 440 450
QLKVDLAVTR DEAAKLQTKK VKKPTGTRNL YLAREGLIRA GTKAAEGVSA
460 470 480 490 500
ADMAKRERFE LLKHQKLKNQ NIFVSQTRLC LHNLPKAVDD KQLRKLLLEA
510 520 530 540 550
TRGEKGVRIK ECRVMRDLKG VHGKMKGQSL GYAFAEFQKH EHALRALRHF
560 570 580 590 600
NNNPEIFGSQ KRPIVEFSLE DRRKLKVKEL RIQRSLQKME SKPVTSKPQK
610 620 630 640 650
EQKEVGKDKQ QKVVQSATQD QSKAPGEQKG KAGSTSWSGF QTKAEVEQVE
660 670 680 690 700
LPDGKKRRKV LALPSHRGPK IRLRDKGKVK SLPSRKPKPQ MGQRKQKKQQ
710 720 730 740 750
LASSVQAPKR KAKENKAEAR FNQLVEQYKQ KLLGPSKGAP LMKRSKWFDS
Length:750
Mass (Da):84,214
Last modified:July 27, 2011 - v4
Checksum:iC767D7C6333E9944
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6SB18F6SB18_MOUSE
RNA-binding protein 28
Rbm28
133Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti239S → I in BAB31653 (PubMed:16141072).Curated1
Sequence conflicti242 – 243DE → GR in BAB31653 (PubMed:16141072).Curated2
Sequence conflicti388L → P in AAH40811 (PubMed:15489334).Curated1
Sequence conflicti484L → F in AAH40811 (PubMed:15489334).Curated1
Sequence conflicti613V → A in BAE33848 (PubMed:16141072).Curated1
Sequence conflicti613V → A in AAH18373 (PubMed:15489334).Curated1
Sequence conflicti613V → A in AAH40811 (PubMed:15489334).Curated1
Sequence conflicti638S → T in BAE33848 (PubMed:16141072).Curated1
Sequence conflicti638S → T in AAH18373 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012408 mRNA Translation: BAC25367.1
AK013047 mRNA Translation: BAB28620.1
AK019296 mRNA Translation: BAB31653.3
AK019436 mRNA Translation: BAC25592.1
AK156773 mRNA Translation: BAE33848.1
AC153909 Genomic DNA No translation available.
BC018373 mRNA Translation: AAH18373.1
BC040811 mRNA Translation: AAH40811.1
CCDSiCCDS51733.1
RefSeqiNP_598686.2, NM_133925.2
UniGeneiMm.40802

Genome annotation databases

EnsembliENSMUST00000007993; ENSMUSP00000007993; ENSMUSG00000029701
GeneIDi68272
KEGGimmu:68272
UCSCiuc009bcx.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012408 mRNA Translation: BAC25367.1
AK013047 mRNA Translation: BAB28620.1
AK019296 mRNA Translation: BAB31653.3
AK019436 mRNA Translation: BAC25592.1
AK156773 mRNA Translation: BAE33848.1
AC153909 Genomic DNA No translation available.
BC018373 mRNA Translation: AAH18373.1
BC040811 mRNA Translation: AAH40811.1
CCDSiCCDS51733.1
RefSeqiNP_598686.2, NM_133925.2
UniGeneiMm.40802

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X4HNMR-A317-414[»]
ProteinModelPortaliQ8CGC6
SMRiQ8CGC6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212775, 3 interactors
DIPiDIP-58949N
IntActiQ8CGC6, 1 interactor
STRINGi10090.ENSMUSP00000007993

PTM databases

iPTMnetiQ8CGC6
PhosphoSitePlusiQ8CGC6
SwissPalmiQ8CGC6

Proteomic databases

EPDiQ8CGC6
MaxQBiQ8CGC6
PaxDbiQ8CGC6
PeptideAtlasiQ8CGC6
PRIDEiQ8CGC6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007993; ENSMUSP00000007993; ENSMUSG00000029701
GeneIDi68272
KEGGimmu:68272
UCSCiuc009bcx.2 mouse

Organism-specific databases

CTDi55131
MGIiMGI:2655711 Rbm28

Phylogenomic databases

eggNOGiKOG0127 Eukaryota
ENOG410XSHP LUCA
GeneTreeiENSGT00550000074976
HOVERGENiHBG073213
InParanoidiQ8CGC6
KOiK14573
OMAiVGCAFVQ
OrthoDBiEOG091G0JKS
TreeFamiTF312798

Miscellaneous databases

ChiTaRSiRbm28 mouse
EvolutionaryTraceiQ8CGC6
PROiPR:Q8CGC6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029701 Expressed in 277 organ(s), highest expression level in testis
CleanExiMM_RBM28
ExpressionAtlasiQ8CGC6 baseline and differential
GenevisibleiQ8CGC6 MM

Family and domain databases

Gene3Di3.30.70.330, 4 hits
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 4 hits
SMARTiView protein in SMART
SM00360 RRM, 4 hits
SUPFAMiSSF54928 SSF54928, 4 hits
PROSITEiView protein in PROSITE
PS50102 RRM, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiRBM28_MOUSE
AccessioniPrimary (citable) accession number: Q8CGC6
Secondary accession number(s): E9QKF8
, Q3U0K5, Q8BG25, Q8VEJ8, Q9CS22, Q9CSE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 129 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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