UniProtKB - Q8CG70 (P3H3_MOUSE)
Prolyl 3-hydroxylase 3
P3h3
Functioni
Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (PubMed:27119146).
Required for normal hydroxylation of lysine residues in type I collagen chains in skin, bone, tendon, aorta and cornea (PubMed:28115524).
Required for normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly (PubMed:27119146, PubMed:28115524).
Apparently not required for normal prolyl 3-hydroxylation on collagen chains, possibly because it functions redundantly with other prolyl 3-hydroxylases (PubMed:28115524).
2 PublicationsCatalytic activityi
- EC:1.14.11.7
Cofactori
Protein has several cofactor binding sites:- Fe cationBy similarity
- L-ascorbateBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 580 | Iron | 1 | |
Metal bindingi | 582 | Iron | 1 | |
Metal bindingi | 652 | Iron | 1 | |
Active sitei | 662 | By similarity | 1 |
GO - Molecular functioni
- iron ion binding Source: InterPro
- L-ascorbic acid binding Source: UniProtKB-KW
- procollagen-proline 3-dioxygenase activity Source: GO_Central
GO - Biological processi
- collagen biosynthetic process Source: UniProtKB
- collagen metabolic process Source: GO_Central
- negative regulation of cell population proliferation Source: MGI
- peptidyl-lysine hydroxylation Source: UniProtKB
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Ligand | Iron, Metal-binding, Vitamin C |
Enzyme and pathway databases
BRENDAi | 1.14.11.7, 3474 |
Reactomei | R-MMU-1650814, Collagen biosynthesis and modifying enzymes |
Names & Taxonomyi
Protein namesi | Recommended name: Prolyl 3-hydroxylase 3Imported (EC:1.14.11.7)Alternative name(s): Leprecan-like protein 21 Publication Protein BBy similarity |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1315208, P3h3 |
VEuPathDBi | HostDB:ENSMUSG00000023191 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum PROSITE-ProRule annotation
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Other locations
- catalytic complex Source: UniProtKB
Keywords - Cellular componenti
Endoplasmic reticulumPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000240361 | 20 – 732 | Prolyl 3-hydroxylase 3Add BLAST | 713 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 327 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 458 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
MaxQBi | Q8CG70 |
PaxDbi | Q8CG70 |
PeptideAtlasi | Q8CG70 |
PRIDEi | Q8CG70 |
ProteomicsDBi | 287749 |
PTM databases
GlyGeni | Q8CG70, 2 sites |
PhosphoSitePlusi | Q8CG70 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000023191, Expressed in humerus cartilage element and 260 other tissues |
ExpressionAtlasi | Q8CG70, baseline and differential |
Genevisiblei | Q8CG70, MM |
Interactioni
Subunit structurei
Identified in a complex with PLOD1 and P3H4.
1 PublicationProtein-protein interaction databases
BioGRIDi | 200050, 2 interactors |
STRINGi | 10090.ENSMUSP00000023958 |
Miscellaneous databases
RNActi | Q8CG70, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 39 – 72 | TPR 1Add BLAST | 34 | |
Repeati | 152 – 185 | TPR 2Add BLAST | 34 | |
Repeati | 214 – 247 | TPR 3Add BLAST | 34 | |
Repeati | 312 – 345 | TPR 4Add BLAST | 34 | |
Domaini | 557 – 671 | Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST | 115 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 15 – 35 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 676 – 732 | DisorderedSequence analysisAdd BLAST | 57 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 674 – 703 | Sequence analysisAdd BLAST | 30 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 729 – 732 | Prevents secretion from ERPROSITE-ProRule annotation | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 15 – 32 | Pro residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 684 – 701 | Acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 702 – 732 | Basic and acidic residuesSequence analysisAdd BLAST | 31 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Repeat, Signal, TPR repeatPhylogenomic databases
eggNOGi | KOG4459, Eukaryota |
GeneTreei | ENSGT00940000159164 |
HOGENOMi | CLU_017820_0_0_1 |
InParanoidi | Q8CG70 |
OMAi | KLYRDME |
OrthoDBi | 660619at2759 |
PhylomeDBi | Q8CG70 |
TreeFami | TF320837 |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR005123, Oxoglu/Fe-dep_dioxygenase IPR039575, P3H IPR039839, P3H3 IPR006620, Pro_4_hyd_alph IPR044862, Pro_4_hyd_alph_FE2OG_OXY IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR14049, PTHR14049, 1 hit PTHR14049:SF14, PTHR14049:SF14, 1 hit |
Pfami | View protein in Pfam PF13640, 2OG-FeII_Oxy_3, 1 hit |
SMARTi | View protein in SMART SM00702, P4Hc, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit PS51471, FE2OG_OXY, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MLRLLRLLLL LLLPPPGSPE PPEPPGLAQL SPGSPPQAPD LLYADGLRAY
60 70 80 90 100
SAGAWAPAVA LLREALRSRA ALGRARQECG ASCAAEPGAA LPSQLLGAPH
110 120 130 140 150
PVSGPGVWEP LLLRATLRRA ECLTQCAVRR LGPGGAARLR VGSALRDAFR
160 170 180 190 200
RREPYNYLQR AYYQLKKLDL AASAAHTFFV ANPTHLQMRE DMAKYRRMSA
210 220 230 240 250
IRPQSFRDLV TPLYWAAYDT GLELLEQREA ALALPQLEEA LQGSLAHMES
260 270 280 290 300
CRAACEGPEE HQGAEEEGEG SQGGLYEAIA GHWIRVLQCR QHCVADTATR
310 320 330 340 350
PGRSFPVQDF LLSQLRRLHE AYAQVGNMSQ AMENVLSVLL FYPEDEAAKK
360 370 380 390 400
ALNQYQTQLG EPRPDLGPRE DIQRFILRSL GEKRQLYYAM EHLGTSFKDP
410 420 430 440 450
DSWTPEALIP KALRERLRED QEKKPWDHQP PQQKPLAHWK DALLMEGVTL
460 470 480 490 500
TQDAQQLNGS ERAVLDGLLT SAECGVLLQL AKDAAQAGAR SGYRGRRSPH
510 520 530 540 550
SPHERFEGLT VLKAAQLARA GTVGRPGAKL LLEVSERVRT LTQAYFSPER
560 570 580 590 600
PLHLSFTHLV CRSAIEGEQE QRMDLSHPVH ADNCVLDPDT GECWREPPAY
610 620 630 640 650
TYRDYSGLLY LNDDFKGGDL FFTQPNALTV TAQVRPRCGR LVAFSSGGEN
660 670 680 690 700
PHGVWAVTRG RRCALALWHT WAPEHSEQEW TEAKELLQEE EEEEEEEDIL
710 720 730
SRDPSPEPPS HKLQRVQEKA GKPRRVRVRE EL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD6RHQ3 | D6RHQ3_MOUSE | Prolyl 3-hydroxylase 3 | P3h3 | 337 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ441086 mRNA Translation: CAD29580.1 AY463530 mRNA Translation: AAS45239.1 BC003726 mRNA Translation: AAH03726.1 BC016431 mRNA Translation: AAH16431.1 AC002397 Genomic DNA Translation: AAC36012.1 |
CCDSi | CCDS20533.1 |
RefSeqi | NP_038562.2, NM_013534.4 |
Genome annotation databases
Ensembli | ENSMUST00000023958; ENSMUSP00000023958; ENSMUSG00000023191 |
GeneIDi | 14789 |
KEGGi | mmu:14789 |
UCSCi | uc009dsf.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ441086 mRNA Translation: CAD29580.1 AY463530 mRNA Translation: AAS45239.1 BC003726 mRNA Translation: AAH03726.1 BC016431 mRNA Translation: AAH16431.1 AC002397 Genomic DNA Translation: AAC36012.1 |
CCDSi | CCDS20533.1 |
RefSeqi | NP_038562.2, NM_013534.4 |
3D structure databases
AlphaFoldDBi | Q8CG70 |
SMRi | Q8CG70 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 200050, 2 interactors |
STRINGi | 10090.ENSMUSP00000023958 |
PTM databases
GlyGeni | Q8CG70, 2 sites |
PhosphoSitePlusi | Q8CG70 |
Proteomic databases
MaxQBi | Q8CG70 |
PaxDbi | Q8CG70 |
PeptideAtlasi | Q8CG70 |
PRIDEi | Q8CG70 |
ProteomicsDBi | 287749 |
Protocols and materials databases
Antibodypediai | 21590, 118 antibodies from 20 providers |
DNASUi | 14789 |
Genome annotation databases
Ensembli | ENSMUST00000023958; ENSMUSP00000023958; ENSMUSG00000023191 |
GeneIDi | 14789 |
KEGGi | mmu:14789 |
UCSCi | uc009dsf.1, mouse |
Organism-specific databases
CTDi | 10536 |
MGIi | MGI:1315208, P3h3 |
VEuPathDBi | HostDB:ENSMUSG00000023191 |
Phylogenomic databases
eggNOGi | KOG4459, Eukaryota |
GeneTreei | ENSGT00940000159164 |
HOGENOMi | CLU_017820_0_0_1 |
InParanoidi | Q8CG70 |
OMAi | KLYRDME |
OrthoDBi | 660619at2759 |
PhylomeDBi | Q8CG70 |
TreeFami | TF320837 |
Enzyme and pathway databases
BRENDAi | 1.14.11.7, 3474 |
Reactomei | R-MMU-1650814, Collagen biosynthesis and modifying enzymes |
Miscellaneous databases
BioGRID-ORCSi | 14789, 0 hits in 72 CRISPR screens |
ChiTaRSi | P3h3, mouse |
PROi | PR:Q8CG70 |
RNActi | Q8CG70, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000023191, Expressed in humerus cartilage element and 260 other tissues |
ExpressionAtlasi | Q8CG70, baseline and differential |
Genevisiblei | Q8CG70, MM |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR005123, Oxoglu/Fe-dep_dioxygenase IPR039575, P3H IPR039839, P3H3 IPR006620, Pro_4_hyd_alph IPR044862, Pro_4_hyd_alph_FE2OG_OXY IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR14049, PTHR14049, 1 hit PTHR14049:SF14, PTHR14049:SF14, 1 hit |
Pfami | View protein in Pfam PF13640, 2OG-FeII_Oxy_3, 1 hit |
SMARTi | View protein in SMART SM00702, P4Hc, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit PS51471, FE2OG_OXY, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | P3H3_MOUSE | |
Accessioni | Q8CG70Primary (citable) accession number: Q8CG70 Secondary accession number(s): O88836 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 27, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | May 25, 2022 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families