UniProtKB - Q8CG47 (SMC4_MOUSE)
Protein
Structural maintenance of chromosomes protein 4
Gene
Smc4
Organism
Mus musculus (Mouse)
Status
Functioni
Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 111 – 118 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATPase activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- chromatin binding Source: MGI
- single-stranded DNA binding Source: MGI
GO - Biological processi
- cell division Source: UniProtKB-KW
- kinetochore organization Source: MGI
- meiotic chromosome condensation Source: MGI
- meiotic chromosome segregation Source: MGI
- mitotic chromosome condensation Source: UniProtKB
- single strand break repair Source: MGI
Keywordsi
Biological process | Cell cycle, Cell division, DNA condensation, Mitosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2299718, Condensation of Prophase Chromosomes R-MMU-2514853, Condensation of Prometaphase Chromosomes |
Names & Taxonomyi
Protein namesi | Recommended name: Structural maintenance of chromosomes protein 4Short name: SMC protein 4 Short name: SMC-4 Alternative name(s): Chromosome-associated polypeptide C XCAP-C homolog |
Gene namesi | Name:Smc4 Synonyms:Capc, Smc4l1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1917349, Smc4 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Nucleus
- Nucleus By similarity
Other locations
- Chromosome By similarity
Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase (By similarity).By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nuclear speck Source: MGI
- nucleus Source: MGI
Other locations
- chromosome, centromeric region Source: MGI
- condensin complex Source: MGI
Keywords - Cellular componenti
Chromosome, Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119008 | 1 – 1286 | Structural maintenance of chromosomes protein 4Add BLAST | 1286 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 21 | PhosphoserineBy similarity | 1 | |
Modified residuei | 27 | PhosphoserineBy similarity | 1 | |
Modified residuei | 40 | PhosphoserineBy similarity | 1 | |
Modified residuei | 141 | PhosphoserineBy similarity | 1 | |
Modified residuei | 379 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 677 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 980 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q8CG47 |
jPOSTi | Q8CG47 |
MaxQBi | Q8CG47 |
PaxDbi | Q8CG47 |
PeptideAtlasi | Q8CG47 |
PRIDEi | Q8CG47 |
ProteomicsDBi | 261520 |
PTM databases
iPTMneti | Q8CG47 |
PhosphoSitePlusi | Q8CG47 |
SwissPalmi | Q8CG47 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000034349, Expressed in telencephalon and 344 other tissues |
Genevisiblei | Q8CG47, MM |
Interactioni
Subunit structurei
Forms a heterodimer with SMC2.
Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG (By similarity).
By similarityBinary interactionsi
Q8CG47
With | #Exp. | IntAct |
---|---|---|
Yy1 [Q00899] | 2 | EBI-6921575,EBI-6921536 |
Protein-protein interaction databases
BioGRIDi | 213863, 22 interactors |
ComplexPortali | CPX-980, Condensin I complex CPX-986, Condensin II complex |
CORUMi | Q8CG47 |
IntActi | Q8CG47, 10 interactors |
MINTi | Q8CG47 |
STRINGi | 10090.ENSMUSP00000047872 |
Miscellaneous databases
RNActi | Q8CG47, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8CG47 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8CG47 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 611 – 725 | SMC hingeAdd BLAST | 115 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 270 – 589 | Sequence analysisAdd BLAST | 320 | |
Coiled coili | 768 – 1018 | Sequence analysisAdd BLAST | 251 | |
Coiled coili | 1068 – 1133 | Sequence analysisAdd BLAST | 66 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 64 – 69 | Poly-Pro | 6 | |
Compositional biasi | 1189 – 1224 | Ala/Asp-rich (DA-box)Add BLAST | 36 |
Domaini
The SMC hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC2, forming a V-shaped heterodimer.By similarity
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0996, Eukaryota |
GeneTreei | ENSGT00900000141094 |
InParanoidi | Q8CG47 |
OrthoDBi | 326079at2759 |
PhylomeDBi | Q8CG47 |
TreeFami | TF101158 |
Family and domain databases
CDDi | cd03274, ABC_SMC4_euk, 2 hits |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR003395, RecF/RecN/SMC_N IPR024704, SMC IPR041738, SMC4_ABC_euk IPR010935, SMC_hinge IPR036277, SMC_hinge_sf |
Pfami | View protein in Pfam PF06470, SMC_hinge, 1 hit PF02463, SMC_N, 1 hit |
PIRSFi | PIRSF005719, SMC, 1 hit |
SMARTi | View protein in SMART SM00968, SMC_hinge, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF75553, SSF75553, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Q8CG47-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRRKGTKPST ACHQEEGPPP SQDGAHSDEE MEQPAGEAES AAPAKPPGEE
60 70 80 90 100
LDNRSLEEIL NSIPPPPPPA MASEAGAPRL MITHIVNQNF KSYAGEKVLG
110 120 130 140 150
PFHKRFSCII GPNGSGKSNV IDSMLFVFGY RAQKIRSKKL SVLIHNSDEH
160 170 180 190 200
KDIQSCTVEV HFQKIIDKEG DDYEVLPNSN FYVSRTAYRD STSVYHISGK
210 220 230 240 250
KKTFKDVGNL LRSHGIDLDH NRFLILQGEV EQIAMMKPKG QTEHDEGMLE
260 270 280 290 300
YLEDIIGCGR LNEPIKVLCR RVEILNEHRG EKLNRVKMVE KEKDALEGEK
310 320 330 340 350
NIAIEFLTLE NEMFKKKNHI CQYYIYDLQN RIAEITTQKE KIHEDTKEIT
360 370 380 390 400
EKSNVLSNEM KAKNSAVKDV EKKLNKVTKF IEQNKEKFTQ LDLEDVQVRE
410 420 430 440 450
KLKHATSKAK KLEKQLQKDK EKVEELKSVP AKSKTVINET TTRNNSLEKE
460 470 480 490 500
REKEEKKLKE VMDSLKQETQ GLQKEKEIQE KELMGFNKSV NEARSKMEVA
510 520 530 540 550
QSELDIYLSR HNTAVSQLSK AKEALITASE TLKERKAAIK DINTKLPQTQ
560 570 580 590 600
QELKEKEKEL QKLTQEEINL KSLVHDLFQK VEEAKSSLAM NRSRGKVLDA
610 620 630 640 650
IIQEKKSGRI PGIYGRLGDL GAIDEKYDIA ISSCCHALDY IVVDSIDTAQ
660 670 680 690 700
ECVNFLKKHN IGIATFIGLD KMTVWAKKMS KIQTPENTPR LFDLVKVKNE
710 720 730 740 750
EIRQAFYFAL RDTLVANNLD QATRVAYQRD RRWRVVTLQG QIIEQSGTMS
760 770 780 790 800
GGGSKVMRGR MGSSVIDEIS VEEVNKMESQ LERHSKQAMQ IQEQKVQHEE
810 820 830 840 850
AVVKLRHSER DMRNTLEKFA ASIQGLSEQE EYLCVQIKEL EANVLTTAPD
860 870 880 890 900
RKQQKLLEEN VSVFKKEYDA VAEKAGKVEA EIKRLHNTII DINNRKLKAQ
910 920 930 940 950
QNKLDTINKQ LDECASAITK AQVAIKTADR NLKKAQDSVC RTEKEIKDTE
960 970 980 990 1000
KEINDLKTEL KNIEDKAEEV INNTKTAETS LPEIQKEHRN LLQELKVIQE
1010 1020 1030 1040 1050
NEHALQKDAL SIKLKLEQID GHISEHNSKI KYWQKEISKI KLHPVEDNPV
1060 1070 1080 1090 1100
ETVAVLSQEE LEAIKNPESI TNEIALLEAQ CREMKPNLGA IAEYKKKEDL
1110 1120 1130 1140 1150
YLQRVAELDK ITSERDNFRQ AYEDLRKQRL NEFMAGFYVI TNKLKENYQM
1160 1170 1180 1190 1200
LTLGGDAELE LVDSLDPFSE GIMFSVRPPK KSWKKIFNLS GGEKTLSSLA
1210 1220 1230 1240 1250
LVFALHHYKP TPLYFMDEID AALDFKNVSI VAFYIYEQTK NAQFIIISLR
1260 1270 1280
NNMFEISDRL IGIYKTYNST KSVAVNPKQI ASKGLC
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q2X6 | E9Q2X6_MOUSE | Structural maintenance of chromosom... | Smc4 | 1,261 | Annotation score: | ||
Q3U763 | Q3U763_MOUSE | Structural maintenance of chromosom... | Smc4 Smc4l1 | 337 | Annotation score: | ||
A0A0A6YXY3 | A0A0A6YXY3_MOUSE | Structural maintenance of chromosom... | Smc4 | 109 | Annotation score: | ||
A0A0A6YX07 | A0A0A6YX07_MOUSE | Structural maintenance of chromosom... | Smc4 | 56 | Annotation score: | ||
D6RCX2 | D6RCX2_MOUSE | Structural maintenance of chromosom... | Smc4 | 46 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 454 | E → V in BAC40608 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ534940 mRNA Translation: CAD59183.1 BC005507 mRNA Translation: AAH05507.1 BC062939 mRNA Translation: AAH62939.1 AK088350 mRNA Translation: BAC40297.1 AK088846 mRNA Translation: BAC40608.2 |
CCDSi | CCDS17401.1 |
RefSeqi | NP_598547.1, NM_133786.3 XP_006502109.1, XM_006502046.2 |
Genome annotation databases
Ensembli | ENSMUST00000042901; ENSMUSP00000047872; ENSMUSG00000034349 |
GeneIDi | 70099 |
KEGGi | mmu:70099 |
UCSCi | uc008pma.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ534940 mRNA Translation: CAD59183.1 BC005507 mRNA Translation: AAH05507.1 BC062939 mRNA Translation: AAH62939.1 AK088350 mRNA Translation: BAC40297.1 AK088846 mRNA Translation: BAC40608.2 |
CCDSi | CCDS17401.1 |
RefSeqi | NP_598547.1, NM_133786.3 XP_006502109.1, XM_006502046.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3L51 | X-ray | 1.51 | B | 595-752 | [»] | |
SMRi | Q8CG47 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 213863, 22 interactors |
ComplexPortali | CPX-980, Condensin I complex CPX-986, Condensin II complex |
CORUMi | Q8CG47 |
IntActi | Q8CG47, 10 interactors |
MINTi | Q8CG47 |
STRINGi | 10090.ENSMUSP00000047872 |
PTM databases
iPTMneti | Q8CG47 |
PhosphoSitePlusi | Q8CG47 |
SwissPalmi | Q8CG47 |
Proteomic databases
EPDi | Q8CG47 |
jPOSTi | Q8CG47 |
MaxQBi | Q8CG47 |
PaxDbi | Q8CG47 |
PeptideAtlasi | Q8CG47 |
PRIDEi | Q8CG47 |
ProteomicsDBi | 261520 |
Protocols and materials databases
Antibodypediai | 18504, 244 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000042901; ENSMUSP00000047872; ENSMUSG00000034349 |
GeneIDi | 70099 |
KEGGi | mmu:70099 |
UCSCi | uc008pma.1, mouse |
Organism-specific databases
CTDi | 10051 |
MGIi | MGI:1917349, Smc4 |
Phylogenomic databases
eggNOGi | KOG0996, Eukaryota |
GeneTreei | ENSGT00900000141094 |
InParanoidi | Q8CG47 |
OrthoDBi | 326079at2759 |
PhylomeDBi | Q8CG47 |
TreeFami | TF101158 |
Enzyme and pathway databases
Reactomei | R-MMU-2299718, Condensation of Prophase Chromosomes R-MMU-2514853, Condensation of Prometaphase Chromosomes |
Miscellaneous databases
BioGRID-ORCSi | 70099, 23 hits in 54 CRISPR screens |
ChiTaRSi | Smc4, mouse |
EvolutionaryTracei | Q8CG47 |
PROi | PR:Q8CG47 |
RNActi | Q8CG47, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000034349, Expressed in telencephalon and 344 other tissues |
Genevisiblei | Q8CG47, MM |
Family and domain databases
CDDi | cd03274, ABC_SMC4_euk, 2 hits |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR003395, RecF/RecN/SMC_N IPR024704, SMC IPR041738, SMC4_ABC_euk IPR010935, SMC_hinge IPR036277, SMC_hinge_sf |
Pfami | View protein in Pfam PF06470, SMC_hinge, 1 hit PF02463, SMC_N, 1 hit |
PIRSFi | PIRSF005719, SMC, 1 hit |
SMARTi | View protein in SMART SM00968, SMC_hinge, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF75553, SSF75553, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SMC4_MOUSE | |
Accessioni | Q8CG47Primary (citable) accession number: Q8CG47 Secondary accession number(s): Q8BTS7, Q8BTY9, Q99K21 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 23, 2003 |
Last sequence update: | March 1, 2003 | |
Last modified: | April 7, 2021 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families