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Protein

Complement C1r-A subcomponent

Gene

C1ra

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.

Catalytic activityi

Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form the active form of C1s (EC 3.4.21.42).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei501Charge relay systemBy similarity1
Active sitei559Charge relay systemBy similarity1
Active sitei656Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processComplement pathway, Immunity, Innate immunity

Enzyme and pathway databases

ReactomeiR-MMU-166663 Initial triggering of complement
R-MMU-173623 Classical antibody-mediated complement activation
R-MMU-977606 Regulation of Complement cascade

Protein family/group databases

MEROPSiS01.209

Names & Taxonomyi

Protein namesi
Recommended name:
Complement C1r-A subcomponent (EC:3.4.21.41)
Alternative name(s):
Complement component 1 subcomponent r-A
Cleaved into the following 2 chains:
Gene namesi
Name:C1ra
Synonyms:C1r
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1355313 C1ra

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16By similarityAdd BLAST16
ChainiPRO_000002758017 – 707Complement C1r-A subcomponentAdd BLAST691
ChainiPRO_000002758117 – 462Complement C1r-A subcomponent heavy chainBy similarityAdd BLAST446
ChainiPRO_0000027582463 – 707Complement C1r-A subcomponent light chainBy similarityAdd BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi70 ↔ 88By similarity
Glycosylationi124N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi145 ↔ 164By similarity
Disulfide bondi160 ↔ 173By similarity
Modified residuei166(3R)-3-hydroxyasparagineBy similarity1
Disulfide bondi175 ↔ 188By similarity
Disulfide bondi192 ↔ 219By similarity
Modified residuei205Phosphoserine; by CK2By similarity1
Glycosylationi220N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi249 ↔ 267By similarity
Disulfide bondi308 ↔ 357By similarity
Disulfide bondi337 ↔ 370By similarity
Disulfide bondi375 ↔ 428By similarity
Disulfide bondi405 ↔ 446By similarity
Disulfide bondi450 ↔ 579Interchain (between heavy and light chains)PROSITE-ProRule annotation
Glycosylationi583N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi622 ↔ 641By similarity
Disulfide bondi652 ↔ 682By similarity

Post-translational modificationi

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation, Phosphoprotein

Proteomic databases

MaxQBiQ8CG16
PaxDbiQ8CG16
PeptideAtlasiQ8CG16
PRIDEiQ8CG16

PTM databases

iPTMnetiQ8CG16
PhosphoSitePlusiQ8CG16

Expressioni

Gene expression databases

BgeeiENSMUSG00000055172
CleanExiMM_C1R
GenevisibleiQ8CG16 MM

Interactioni

Subunit structurei

C1 is a calcium-dependent trimolecular complex of C1q, C1r and C1s in the molar ration of 1:2:2. C1r is a dimer of identical chains, each of which is activated by cleavage into two chains, A and B, connected by disulfide bonds (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000063707

Structurei

3D structure databases

ProteinModelPortaliQ8CG16
SMRiQ8CG16
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 140CUB 1PROSITE-ProRule annotationAdd BLAST124
Domaini141 – 189EGF-like; calcium-bindingSequence analysisAdd BLAST49
Domaini192 – 304CUB 2PROSITE-ProRule annotationAdd BLAST113
Domaini306 – 372Sushi 1PROSITE-ProRule annotationAdd BLAST67
Domaini373 – 448Sushi 2PROSITE-ProRule annotationAdd BLAST76
Domaini463 – 704Peptidase S1PROSITE-ProRule annotationAdd BLAST242

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118890
HOGENOMiHOG000237311
HOVERGENiHBG000559
InParanoidiQ8CG16
KOiK01330
OMAiQYYCHEP
OrthoDBiEOG091G02DS
PhylomeDBiQ8CG16
TreeFamiTF330373

Family and domain databases

CDDicd00033 CCP, 2 hits
cd00041 CUB, 2 hits
cd00190 Tryp_SPc, 1 hit
Gene3Di2.60.120.290, 2 hits
InterProiView protein in InterPro
IPR035707 Complement_subcomponent_C1r
IPR000859 CUB_dom
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR035914 Sperma_CUB_dom_sf
IPR035976 Sushi/SCR/CCP_sf
IPR000436 Sushi_SCR_CCP_dom
IPR001254 Trypsin_dom
IPR033116 TRYPSIN_SER
PANTHERiPTHR45206 PTHR45206, 1 hit
PfamiView protein in Pfam
PF00431 CUB, 2 hits
PF00084 Sushi, 2 hits
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00032 CCP, 2 hits
SM00042 CUB, 2 hits
SM00181 EGF, 1 hit
SM00179 EGF_CA, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF49854 SSF49854, 2 hits
SSF50494 SSF50494, 1 hit
SSF57535 SSF57535, 2 hits
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 1 hit
PS01180 CUB, 2 hits
PS01186 EGF_2, 1 hit
PS01187 EGF_CA, 1 hit
PS50923 SUSHI, 2 hits
PS50240 TRYPSIN_DOM, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CG16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLFALLVTL FYGVEGSIYL PQKLYGEVTS PLYPKPYPSD LETTTVITVP
60 70 80 90 100
MGYRVKLVFW QFDVEPSEGC FYDYVKISAD KQTLGRFCGQ LDSPLGNPPG
110 120 130 140 150
SKEFMSQGNK MLLTFHTDFS NEENGTIMFY KGFLAYYQAV DLDECASQPN
160 170 180 190 200
SVEEGLQPRC QHLCHNYVGG YFCSCHPGYE LQKDGQSCQA ECSSELYTEP
210 220 230 240 250
SGYVSSLEYP QPYPPDLRCN YSIRVERGLT VHLKFLDPFE IDDHQQVHCP
260 270 280 290 300
YDQLQIYANG KNLGEFCGKQ RPPDLDTSSN AVDLLFFTDE SGDSRGWKLH
310 320 330 340 350
YTTETIKCPQ PKALDEFTII QDPQPQYQFR DYFIVTCKQG YQLMEGNQAL
360 370 380 390 400
LSFTAVCQND GTWHRAMPRC KIKNCGQPQS LSNGDFRYIT TKGVTTYEAS
410 420 430 440 450
IQYHCHEPYY KMLTRAGSSE SMRGIYTCTA QGIWKNEEEG EKMPRCLPVC
460 470 480 490 500
GKPVNPVTQK ERIIRGQPAR PGNFPWQAFT TTHGRGGGAL LGDRWILTAA
510 520 530 540 550
HTIYPKHHNK ENDNANPKML VFLGHTNVEQ IKKLGHHPVR RVIIHPDYRQ
560 570 580 590 600
DEPNNFEGDI ALLELENSVT LGPELLPICL PDNETFYGQG LMGYVSGFGI
610 620 630 640 650
TEDKLAFDLR FVRLPVADSE ACQRWLQTKK DTSPFSQNMF CSGDPAVQQD
660 670 680 690 700
ACQGDSGGVF AVRDRNRDIW VATGIVSWGI GCGEGYGFYT KVLNYVDWIK

KEMGDEN
Length:707
Mass (Da):80,073
Last modified:March 1, 2003 - v1
Checksum:i29DAAEB3C047D8E8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101S → R in AAG01898 (Ref. 1) Curated1
Sequence conflicti359N → H in AAH04637 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148216 mRNA Translation: AAG01898.1
AF459011
, AF459008, AF459009, AF459010 Genomic DNA Translation: AAO15553.1
BC004637 mRNA Translation: AAH04637.1
CCDSiCCDS20521.1
RefSeqiNP_075632.3, NM_023143.3
UniGeneiMm.333375

Genome annotation databases

EnsembliENSMUST00000068593; ENSMUSP00000063707; ENSMUSG00000055172
GeneIDi50909
KEGGimmu:50909
UCSCiuc009dra.2 mouse

Similar proteinsi

Entry informationi

Entry nameiC1RA_MOUSE
AccessioniPrimary (citable) accession number: Q8CG16
Secondary accession number(s): Q99KI6, Q9ET60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2003
Last modified: February 28, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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