UniProtKB - Q8CG09 (MRP1_RAT)
Multidrug resistance-associated protein 1
Abcc1
Functioni
Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export. Hydrolyzes ATP with low efficiency (PubMed:15129170).
Catalyzes the export of sphingosine 1-phosphate from mast cells independently of their degranulation (PubMed:17050692).
Participates in inflammatory response by allowing export of leukotriene C4 from leukotriene C4-synthezing cells (By similarity).
By similarity2 PublicationsCatalytic activityi
- an S-substituted glutathione(in) + ATP + H2O = ADP + an S-substituted glutathione(out) + H+ + phosphateBy similarityEC:7.6.2.3By similarityThis reaction proceeds in the forwardBy similarity direction.
- ATP + H2O + sphing-4-enine 1-phosphate(in) = ADP + H+ + phosphate + sphing-4-enine 1-phosphate(out)1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
- 17β-estradiol 17-O-(β-D-glucuronate)(in) + ATP + H2O = 17β-estradiol 17-O-(β-D-glucuronate)(out) + ADP + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2).By similarity EC:7.6.2.2
Activity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 679 – 686 | ATP 1PROSITE-ProRule annotation | 8 | |
Nucleotide bindingi | 1328 – 1335 | ATP 2PROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ABC-type glutathione S-conjugate transporter activity Source: UniProtKB
- ABC-type transporter activity Source: UniProtKB
- ABC-type vitamin B12 transporter activity Source: Ensembl
- ABC-type xenobiotic transporter activity Source: RGD
- amide transmembrane transporter activity Source: RGD
- ATPase-coupled lipid transmembrane transporter activity Source: RGD
- ATPase-coupled transmembrane transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- carboxylic acid transmembrane transporter activity Source: RGD
- efflux transmembrane transporter activity Source: RGD
- glutathione transmembrane transporter activity Source: RGD
- hydrolase activity Source: UniProtKB-KW
- long-chain fatty acid transporter activity Source: RGD
- oxidoreductase activity Source: InterPro
- xenobiotic transmembrane transporter activity Source: UniProtKB
GO - Biological processi
- carboxylic acid transmembrane transport Source: RGD
- cell chemotaxis Source: RGD
- cellular response to amyloid-beta Source: RGD
- cobalamin metabolic process Source: Ensembl
- export across plasma membrane Source: RGD
- glutathione transmembrane transport Source: RGD
- leukotriene transport Source: UniProtKB
- long-chain fatty acid import across plasma membrane Source: RGD
- negative regulation of cell death Source: RGD
- negative regulation of neuron death Source: RGD
- phospholipid efflux Source: RGD
- phospholipid translocation Source: RGD
- positive regulation of cell migration Source: RGD
- positive regulation of inflammatory response Source: UniProtKB
- response to oxidative stress Source: RGD
- response to xenobiotic stimulus Source: RGD
- sphingolipid translocation Source: RGD
- transepithelial transport Source: RGD
- transmembrane transport Source: RGD
- xenobiotic detoxification by transmembrane export across the plasma membrane Source: RGD
- xenobiotic transport Source: RGD
- xenobiotic transport across blood-brain barrier Source: RGD
Keywordsi
Molecular function | Hydrolase, Translocase |
Biological process | Lipid transport, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-189483, Heme degradation R-RNO-196741, Cobalamin (Cbl, vitamin B12) transport and metabolism R-RNO-2142691, Synthesis of Leukotrienes (LT) and Eoxins (EX) R-RNO-382556, ABC-family proteins mediated transport R-RNO-9707564, Cytoprotection by HMOX1 |
Chemistry databases
SwissLipidsi | SLP:000000405 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3112, Abcc1 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein PROSITE-ProRule annotation1 Publication
Plasma Membrane
- apical plasma membrane Source: RGD
- basal plasma membrane Source: ARUK-UCL
- basolateral plasma membrane Source: ARUK-UCL
- integral component of plasma membrane Source: RGD
- lateral plasma membrane Source: RGD
- plasma membrane Source: RGD
Other locations
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 33 | ExtracellularBy similarityAdd BLAST | 33 | |
Transmembranei | 34 – 54 | Helical; Name=1PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 55 – 74 | CytoplasmicBy similarityAdd BLAST | 20 | |
Transmembranei | 75 – 95 | Helical; Name=2PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 96 – 100 | ExtracellularBy similarity | 5 | |
Transmembranei | 101 – 121 | Helical; Name=3PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 122 – 133 | CytoplasmicBy similarityAdd BLAST | 12 | |
Transmembranei | 134 – 154 | Helical; Name=4PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 155 – 172 | ExtracellularBy similarityAdd BLAST | 18 | |
Transmembranei | 173 – 193 | Helical; Name=5PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 194 – 317 | CytoplasmicBy similarityAdd BLAST | 124 | |
Transmembranei | 318 – 338 | Helical; Name=6PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 339 – 364 | ExtracellularBy similarityAdd BLAST | 26 | |
Transmembranei | 365 – 385 | Helical; Name=7PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 386 – 441 | CytoplasmicBy similarityAdd BLAST | 56 | |
Transmembranei | 442 – 462 | Helical; Name=8PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 463 – 465 | ExtracellularBy similarity | 3 | |
Transmembranei | 466 – 486 | Helical; Name=9PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 487 – 548 | CytoplasmicBy similarityAdd BLAST | 62 | |
Transmembranei | 549 – 569 | Helical; Name=10PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 570 – 591 | ExtracellularBy similarityAdd BLAST | 22 | |
Transmembranei | 592 – 612 | Helical; Name=11PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 613 – 967 | CytoplasmicBy similarityAdd BLAST | 355 | |
Transmembranei | 968 – 988 | Helical; Name=12PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 989 – 1026 | ExtracellularBy similarityAdd BLAST | 38 | |
Transmembranei | 1027 – 1047 | Helical; Name=13PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1048 – 1090 | CytoplasmicBy similarityAdd BLAST | 43 | |
Transmembranei | 1091 – 1111 | Helical; Name=14PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1112 | ExtracellularBy similarity | 1 | |
Transmembranei | 1113 – 1133 | Helical; Name=15PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1134 – 1204 | CytoplasmicBy similarityAdd BLAST | 71 | |
Transmembranei | 1205 – 1225 | Helical; Name=16PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1226 – 1227 | ExtracellularBy similarity | 2 | |
Transmembranei | 1228 – 1248 | Helical; Name=17PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1249 – 1532 | CytoplasmicBy similarityAdd BLAST | 284 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 983 | L → M: No effect on estradiol glucuronide transport. 1 Publication | 1 | |
Mutagenesisi | 1090 | Q → E: 7.6-fold increase of the estradiol glucuronide transport; when associated with T-1243. Increases doxorubicin inhibition. 1 Publication | 1 | |
Mutagenesisi | 1101 | S → N: 50% increase of estradiol glucuronide transport. 1 Publication | 1 | |
Mutagenesisi | 1106 | V → C: No effect on estradiol glucuronide transport. 1 Publication | 1 | |
Mutagenesisi | 1243 | A → T: 7.6-fold increase of the estradiol glucuronide transport; when associated with E-1090. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL5680 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093354 | 1 – 1532 | Multidrug resistance-associated protein 1Add BLAST | 1532 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 19 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 277 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 290 | PhosphoserineBy similarity | 1 | |
Modified residuei | 504 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 879 | PhosphoserineCombined sources | 1 | |
Modified residuei | 883 | PhosphoserineBy similarity | 1 | |
Modified residuei | 916 | PhosphoserineBy similarity | 1 | |
Modified residuei | 931 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | Q8CG09 |
PaxDbi | Q8CG09 |
PTM databases
GlyGeni | Q8CG09, 1 site |
iPTMneti | Q8CG09 |
PhosphoSitePlusi | Q8CG09 |
Expressioni
Tissue specificityi
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 326 – 609 | ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST | 284 | |
Domaini | 645 – 869 | ABC transporter 1PROSITE-ProRule annotationAdd BLAST | 225 | |
Domaini | 975 – 1257 | ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST | 283 | |
Domaini | 1294 – 1528 | ABC transporter 2PROSITE-ProRule annotationAdd BLAST | 235 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0054, Eukaryota |
InParanoidi | Q8CG09 |
OrthoDBi | 138195at2759 |
PhylomeDBi | Q8CG09 |
Family and domain databases
Gene3Di | 1.20.1560.10, 2 hits 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR018170, Aldo/ket_reductase_CS IPR005292, Multidrug-R_assoc IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00664, ABC_membrane, 2 hits PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF90123, SSF90123, 2 hits |
TIGRFAMsi | TIGR00957, MRP_assoc_pro, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 2 hits PS00211, ABC_TRANSPORTER_1, 2 hits PS50893, ABC_TRANSPORTER_2, 2 hits PS00063, ALDOKETO_REDUCTASE_3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MALSSFCSSD GSDPLWDWNV TWHTSNPDFT KCFQNTVLTW VPCFYLWSCF
60 70 80 90 100
PLYFLYLSRH DRGYIQMTHL NKAKTALGFF LWIICWADLF YSFWERSQGM
110 120 130 140 150
LLAPVLLVSP TLLGITMLLA TFLIQFERRK GVQSSGIMLT FWLVALLCAL
160 170 180 190 200
AILRSKIISA LKKDAQVDMF RDSAFYLYFT LVFIQLVLSC FSDSSPLFSE
210 220 230 240 250
TVRDPNPCPE SSASFLSRIT FWWITGMMVQ GYRQPLKSSD LWSLNKEDTS
260 270 280 290 300
EEVVPVLVNN WKKECVKSRK QPVRIVYAPP KDPTKPKGSS QLDVNEEVEA
310 320 330 340 350
LIVKSSHKDR DPSLFKVLYK TFGPYFLMSF LYKALHDLMM FAGPEILELI
360 370 380 390 400
INFVNDREAP DWQGYLYTAL LFVSACLQTL ALHQYFHICF VTGMRIKTAV
410 420 430 440 450
VGAVYRKALV ITNSARKSST VGEIVNLMSV DAQRFMDLAT YINMIWSAPL
460 470 480 490 500
QVTLALYFLW LNLGPSVLAG VAVMILMVPF NAVMAMKTKT YQVAHMKSKD
510 520 530 540 550
NRIKLMNEIL NGIKVLKLYA WELAFQDKVM NIRQEELKVL KKSAYLAAVG
560 570 580 590 600
TFTWVCTPFL VALSTFAVFV TVDEKNILDA KKAFVSLALF NILRFPLNIL
610 620 630 640 650
PMVISSIVQA SVSLKRLRIF LSHEELEPDS IERWSIKDGG GMNSITVKNA
660 670 680 690 700
TFTWARDEPP TLNGITFAIP DGALVAVVGQ VGCGKSSLLS ALLAEMDKVE
710 720 730 740 750
GHVTLKGSVA YVPQQAWIQN DSLRENILFG RPLQEHCYKA VMEACALLPD
760 770 780 790 800
LEILPSGDLT EIGEKGVNLS GGQKQRVSLA RAVYCNSDIY LLDDPLSAVD
810 820 830 840 850
AHVGKHIFEK VVGPMGLLKN KTRILVTHGI SYLPQVDVII VMSGGKISEM
860 870 880 890 900
GSYQELLDRD GAFAEFVRTY ANTEQDLASE DDSKNGVSGL GKESKPVENG
910 920 930 940 950
ILVTDAVGKP LQRHLSNSSS HSVVTNQQHS STAELQKSGV KEETWKLMEA
960 970 980 990 1000
DKAQTGQVKL SVYWNYMKAI GLCISFLSIF LFLCNHVSAL ASNYWLSLWT
1010 1020 1030 1040 1050
DDRPAVNGTQ ENRNFRLSVY GALGILQGVA VFGYSMAVSI GGIFASRRLH
1060 1070 1080 1090 1100
LDLLQNVLRS PMSFFERTPS GNLVNRFSKE LDTVDSMIPQ VIKMFMGSLF
1110 1120 1130 1140 1150
SVIGAVIIIL LATPIAAVII PPLGLVYFFV QRFYVASSRQ LKRLESVSRS
1160 1170 1180 1190 1200
PVYSHFNETL LGVSVIRAFE EQERFIRQSD LKVDENQKAY YPSIVANRWL
1210 1220 1230 1240 1250
AVRLECVGNC IVLFAALFAV ISRHSLSAGL VGLSVSYSLQ ITAYLNWLVR
1260 1270 1280 1290 1300
MSSEMETNIV AVERLKEYSE TEKEASWQIQ ETAPPSTWPH SGRVEFRDYC
1310 1320 1330 1340 1350
LRYREDLDLV LKHINVTIEG GEKVGIVGRT GAGKSSLTLG LFRINESAEG
1360 1370 1380 1390 1400
EIIIDGINIA KIGLHNLRFK ITIIPQDPVL FSGSLRMNLD PFSQYSDEEV
1410 1420 1430 1440 1450
WMALELAHLK GFVSALPDKL NHECAEGGEN LSVGQRQLVC LARALLRKTK
1460 1470 1480 1490 1500
ILVLDEATAA VDLETDDLIQ STIRTQFEDS TVLTIAHRLN TIMDYTRVIV
1510 1520 1530
LDKGEIRECG APSELLQQRG VFYSMAKDAG LV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE9PT28 | E9PT28_RAT | ABC-type glutathione-S-conjugate tr... | Abcc1 LOC688708 | 1,530 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 4 | S → R in AAN86532 (PubMed:12423064).Curated | 1 | |
Sequence conflicti | 4 | S → R in AAO44983 (Ref. 4) Curated | 1 | |
Sequence conflicti | 480 | F → S in AAO44983 (Ref. 4) Curated | 1 | |
Sequence conflicti | 820 | N → S in CAB97204 (PubMed:11208926).Curated | 1 | |
Sequence conflicti | 868 | R → P in CAB97204 (PubMed:11208926).Curated | 1 | |
Sequence conflicti | 916 | S → G in CAB97204 (PubMed:11208926).Curated | 1 | |
Sequence conflicti | 1122 | P → T in CAB97204 (PubMed:11208926).Curated | 1 | |
Sequence conflicti | 1382 | S → P in AAN86532 (PubMed:12423064).Curated | 1 | |
Sequence conflicti | 1473 | I → V in AAN86532 (PubMed:12423064).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_017015 | 912 – 920 | Missing in isoform 2. 1 Publication | 9 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY170916 mRNA Translation: AAN86532.1 AF487549 mRNA Translation: AAO85437.1 AY174892 mRNA Translation: AAO44983.1 AJ277881 mRNA Translation: CAB97204.1 X96394 mRNA Translation: CAA65258.1 |
RefSeqi | NP_071617.2, NM_022281.2 [Q8CG09-1] |
Genome annotation databases
GeneIDi | 24565 |
KEGGi | rno:24565 |
UCSCi | RGD:3112, rat [Q8CG09-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY170916 mRNA Translation: AAN86532.1 AF487549 mRNA Translation: AAO85437.1 AY174892 mRNA Translation: AAO44983.1 AJ277881 mRNA Translation: CAB97204.1 X96394 mRNA Translation: CAA65258.1 |
RefSeqi | NP_071617.2, NM_022281.2 [Q8CG09-1] |
3D structure databases
SMRi | Q8CG09 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000041695 |
Chemistry databases
ChEMBLi | CHEMBL5680 |
SwissLipidsi | SLP:000000405 |
PTM databases
GlyGeni | Q8CG09, 1 site |
iPTMneti | Q8CG09 |
PhosphoSitePlusi | Q8CG09 |
Proteomic databases
jPOSTi | Q8CG09 |
PaxDbi | Q8CG09 |
Genome annotation databases
GeneIDi | 24565 |
KEGGi | rno:24565 |
UCSCi | RGD:3112, rat [Q8CG09-1] |
Organism-specific databases
CTDi | 4363 |
RGDi | 3112, Abcc1 |
Phylogenomic databases
eggNOGi | KOG0054, Eukaryota |
InParanoidi | Q8CG09 |
OrthoDBi | 138195at2759 |
PhylomeDBi | Q8CG09 |
Enzyme and pathway databases
Reactomei | R-RNO-189483, Heme degradation R-RNO-196741, Cobalamin (Cbl, vitamin B12) transport and metabolism R-RNO-2142691, Synthesis of Leukotrienes (LT) and Eoxins (EX) R-RNO-382556, ABC-family proteins mediated transport R-RNO-9707564, Cytoprotection by HMOX1 |
Miscellaneous databases
PROi | PR:Q8CG09 |
Family and domain databases
Gene3Di | 1.20.1560.10, 2 hits 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR018170, Aldo/ket_reductase_CS IPR005292, Multidrug-R_assoc IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00664, ABC_membrane, 2 hits PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF90123, SSF90123, 2 hits |
TIGRFAMsi | TIGR00957, MRP_assoc_pro, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 2 hits PS00211, ABC_TRANSPORTER_1, 2 hits PS50893, ABC_TRANSPORTER_2, 2 hits PS00063, ALDOKETO_REDUCTASE_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MRP1_RAT | |
Accessioni | Q8CG09Primary (citable) accession number: Q8CG09 Secondary accession number(s): Q63346 Q9JHS0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2006 |
Last sequence update: | January 24, 2006 | |
Last modified: | February 23, 2022 | |
This is version 137 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families