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Protein

GDH/6PGL endoplasmic bifunctional protein

Gene

H6pd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.By similarity

Catalytic activityi

D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H.PROSITE-ProRule annotation
D-glucose 6-phosphate + NAD(P)+ = 6-phospho-D-glucono-1,5-lactone + NAD(P)H.
6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31NADPBy similarity1
Binding sitei63NADPBy similarity1
Binding sitei201SubstratePROSITE-ProRule annotation1
Binding sitei205SubstratePROSITE-ProRule annotation1
Active sitei264Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Oxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
LigandNAD, NADP

Enzyme and pathway databases

SABIO-RKiQ8CFX1

Names & Taxonomyi

Protein namesi
Recommended name:
GDH/6PGL endoplasmic bifunctional protein
Including the following 2 domains:
Glucose 1-dehydrogenase (EC:1.1.1.47)
Alternative name(s):
Glucose-6-phosphate dehydrogenase (EC:1.1.1.363)
6-phosphogluconolactonase (EC:3.1.1.31)
Short name:
6PGL
Gene namesi
Name:H6pd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2140356 H6pd

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000023679417 – 789GDH/6PGL endoplasmic bifunctional proteinAdd BLAST773

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17Pyrrolidone carboxylic acidBy similarity1
Glycosylationi154N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei205N6-succinyllysineCombined sources1
Glycosylationi279N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei424N6-succinyllysineCombined sources1
Glycosylationi681N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

EPDiQ8CFX1
MaxQBiQ8CFX1
PaxDbiQ8CFX1
PRIDEiQ8CFX1

PTM databases

iPTMnetiQ8CFX1
PhosphoSitePlusiQ8CFX1
SwissPalmiQ8CFX1

Expressioni

Gene expression databases

BgeeiENSMUSG00000028980 Expressed in 204 organ(s), highest expression level in white adipose tissue
ExpressionAtlasiQ8CFX1 baseline and differential
GenevisibleiQ8CFX1 MM

Interactioni

Protein-protein interaction databases

IntActiQ8CFX1, 3 interactors
MINTiQ8CFX1
STRINGi10090.ENSMUSP00000030830

Structurei

3D structure databases

ProteinModelPortaliQ8CFX1
SMRiQ8CFX1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni17 – 524Glucose 1-dehydrogenaseAdd BLAST508
Regioni525 – 538LinkerAdd BLAST14
Regioni539 – 7896-phosphogluconolactonaseAdd BLAST251

Sequence similaritiesi

In the N-terminal section; belongs to the glucose-6-phosphate dehydrogenase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0563 Eukaryota
KOG3147 Eukaryota
COG0363 LUCA
COG0364 LUCA
GeneTreeiENSGT00530000063435
HOGENOMiHOG000231077
HOVERGENiHBG005780
InParanoidiQ8CFX1
KOiK13937

Family and domain databases

CDDicd01400 6PGL, 1 hit
InterProiView protein in InterPro
IPR005900 6-phosphogluconolactonase_DevB
IPR001282 G6P_DH
IPR019796 G6P_DH_AS
IPR022675 G6P_DH_C
IPR022674 G6P_DH_NAD-bd
IPR006148 Glc/Gal-6P_isomerase
IPR036291 NAD(P)-bd_dom_sf
IPR037171 NagB/RpiA_transferase-like
PANTHERiPTHR23429 PTHR23429, 1 hit
PfamiView protein in Pfam
PF02781 G6PD_C, 1 hit
PF00479 G6PD_N, 1 hit
PF01182 Glucosamine_iso, 1 hit
PRINTSiPR00079 G6PDHDRGNASE
SUPFAMiSSF100950 SSF100950, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01198 pgl, 1 hit
PROSITEiView protein in PROSITE
PS00069 G6P_DEHYDROGENASE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q8CFX1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLLAAMCLAL LGCLQAQELK GHVSIILLGA TGDLAKKYLW QGLFQLYLDE
60 70 80 90 100
AGKGHSFSFH GAALTAPQQG QKLMDKVLES LSCPKDLVPS RCDELKGQFL
110 120 130 140 150
QLSQYRQLKT VEDYQTLNKD IETQVQQDGL WEAGRIFYFS VPPFAYADIA
160 170 180 190 200
RNINSSCRPH PGAWLRVVFE KPFGHDHLSA QQLASELGSF FQEEEMYRVD
210 220 230 240 250
HYLGKQAVAQ ILPFRDQNRK ALDGLWNRHH VERVEIILKE TIDAEGRASF
260 270 280 290 300
YEEYGVIRDT LQNHLTEILT LVAMELPLNI SSSAAVLQHK LWAFQALRGL
310 320 330 340 350
QKSSAILGQY QAYSGQVRRE LQKPDGFQSL TPTFAGVLVH IDNLRWEGVP
360 370 380 390 400
FILMSGKALD ERVGYVRIVF KNRAYCTQSE RHWVPEQSRC LPQQIIFYIG
410 420 430 440 450
HGELGHPAIL VSRNLFKPSL PTQKWKEVQD QPGLRLFGRP LSDYYAYRPV
460 470 480 490 500
REQDAYSTLL SHIFHCRKES FITTENLLAS WVFWTPLLDS LAFEVPRPYP
510 520 530 540 550
GGAENGQLLD FEFSGGQLTF SQQQLEVLIP DLGSVPKPSD FQVLGARYRQ
560 570 580 590 600
SPLITAWPEE LISKLASDIE AAAVQAVRHF GKFHLALSGG SSPIALFQQL
610 620 630 640 650
ATGHYSFPWA HTHLWLVDER CVPLSDPDSN FQGLQAHLLQ HVRVPYYNIH
660 670 680 690 700
PMPVHLHQRL CAEEDQGAQT YASEISALVA NSSFDLVLLG MGTDGHTASL
710 720 730 740 750
FPQSPTGLDG DQLVVLTESP FRPHQRMSLS LPLINRAKKV AVLVMGRTKR
760 770 780
EITTLVSRVG HEPKKWPISG VVPLSGQLVW YMDYEAFLG
Length:789
Mass (Da):88,928
Last modified:July 27, 2011 - v2
Checksum:iE970CE8B35D3E1E5
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2A7A7A2A7A7_MOUSE
GDH/6PGL endoplasmic bifunctional p...
H6pd
797Annotation score:
A2A7A8A2A7A8_MOUSE
GDH/6PGL endoplasmic bifunctional p...
H6pd
171Annotation score:

Sequence cautioni

The sequence BAC32260 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77V → A in BAE35029 (PubMed:16141072).Curated1
Sequence conflicti77V → A in CAQ51682 (PubMed:19468303).Curated1
Sequence conflicti77V → A in AAH42677 (PubMed:15489334).Curated1
Sequence conflicti106R → H in BAE35029 (PubMed:16141072).Curated1
Sequence conflicti106R → H in CAQ51682 (PubMed:19468303).Curated1
Sequence conflicti106R → H in AAH42677 (PubMed:15489334).Curated1
Sequence conflicti285A → T in BAE35029 (PubMed:16141072).Curated1
Sequence conflicti285A → T in CAQ51682 (PubMed:19468303).Curated1
Sequence conflicti285A → T in AAH42677 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK045199 mRNA Translation: BAC32260.1 Different initiation.
AK159373 mRNA Translation: BAE35029.1
AL606914 Genomic DNA Translation: CAM16119.1
CU463327 Genomic DNA Translation: CAQ51682.1
BC042677 mRNA Translation: AAH42677.1
CCDSiCCDS71524.1
RefSeqiNP_001277933.1, NM_001291004.1
NP_775547.2, NM_173371.4
UniGeneiMm.22183

Genome annotation databases

EnsembliENSMUST00000084117; ENSMUSP00000081134; ENSMUSG00000028980
GeneIDi100198
KEGGimmu:100198
UCSCiuc008vxh.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK045199 mRNA Translation: BAC32260.1 Different initiation.
AK159373 mRNA Translation: BAE35029.1
AL606914 Genomic DNA Translation: CAM16119.1
CU463327 Genomic DNA Translation: CAQ51682.1
BC042677 mRNA Translation: AAH42677.1
CCDSiCCDS71524.1
RefSeqiNP_001277933.1, NM_001291004.1
NP_775547.2, NM_173371.4
UniGeneiMm.22183

3D structure databases

ProteinModelPortaliQ8CFX1
SMRiQ8CFX1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8CFX1, 3 interactors
MINTiQ8CFX1
STRINGi10090.ENSMUSP00000030830

PTM databases

iPTMnetiQ8CFX1
PhosphoSitePlusiQ8CFX1
SwissPalmiQ8CFX1

Proteomic databases

EPDiQ8CFX1
MaxQBiQ8CFX1
PaxDbiQ8CFX1
PRIDEiQ8CFX1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000084117; ENSMUSP00000081134; ENSMUSG00000028980
GeneIDi100198
KEGGimmu:100198
UCSCiuc008vxh.2 mouse

Organism-specific databases

CTDi9563
MGIiMGI:2140356 H6pd

Phylogenomic databases

eggNOGiKOG0563 Eukaryota
KOG3147 Eukaryota
COG0363 LUCA
COG0364 LUCA
GeneTreeiENSGT00530000063435
HOGENOMiHOG000231077
HOVERGENiHBG005780
InParanoidiQ8CFX1
KOiK13937

Enzyme and pathway databases

SABIO-RKiQ8CFX1

Miscellaneous databases

PROiPR:Q8CFX1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028980 Expressed in 204 organ(s), highest expression level in white adipose tissue
ExpressionAtlasiQ8CFX1 baseline and differential
GenevisibleiQ8CFX1 MM

Family and domain databases

CDDicd01400 6PGL, 1 hit
InterProiView protein in InterPro
IPR005900 6-phosphogluconolactonase_DevB
IPR001282 G6P_DH
IPR019796 G6P_DH_AS
IPR022675 G6P_DH_C
IPR022674 G6P_DH_NAD-bd
IPR006148 Glc/Gal-6P_isomerase
IPR036291 NAD(P)-bd_dom_sf
IPR037171 NagB/RpiA_transferase-like
PANTHERiPTHR23429 PTHR23429, 1 hit
PfamiView protein in Pfam
PF02781 G6PD_C, 1 hit
PF00479 G6PD_N, 1 hit
PF01182 Glucosamine_iso, 1 hit
PRINTSiPR00079 G6PDHDRGNASE
SUPFAMiSSF100950 SSF100950, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01198 pgl, 1 hit
PROSITEiView protein in PROSITE
PS00069 G6P_DEHYDROGENASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiG6PE_MOUSE
AccessioniPrimary (citable) accession number: Q8CFX1
Secondary accession number(s): A2A7A9, B2KGW7, Q8BLH1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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