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Protein

Riboflavin kinase

Gene

Rfk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase (By similarity).By similarity

Catalytic activityi

ATP + riboflavin = ADP + FMN.

Cofactori

Zn2+By similarity, Mg2+By similarityNote: Zinc or magnesium.By similarity

Pathwayi: FMN biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes FMN from riboflavin (ATP route).
Proteins known to be involved in this subpathway in this organism are:
  1. Riboflavin kinase (Rfk)
This subpathway is part of the pathway FMN biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes FMN from riboflavin (ATP route), the pathway FMN biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15ATP; via amide nitrogenBy similarity1
Binding sitei21ATP; via amide nitrogenBy similarity1
Metal bindingi27MagnesiumBy similarity1
Binding sitei27ATP; via amide nitrogenBy similarity1
Binding sitei29ATPBy similarity1
Active sitei79NucleophileBy similarity1
Binding sitei82ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei84ATP; via carbonyl oxygenBy similarity1
Binding sitei91ATPBy similarity1
Binding sitei104FMNBy similarity1
Binding sitei107FMN; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei109FMN; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: MGI
  • FMN biosynthetic process Source: UniProtKB-UniPathway
  • positive regulation of NAD(P)H oxidase activity Source: MGI
  • reactive oxygen species metabolic process Source: MGI
  • riboflavin biosynthetic process Source: InterPro

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Flavoprotein, FMN, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-196843 Vitamin B2 (riboflavin) metabolism
UniPathwayiUPA00276; UER00406

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin kinase (EC:2.7.1.26)
Alternative name(s):
ATP:riboflavin 5'-phosphotransferase
Flavokinase
KOI-4
Gene namesi
Name:Rfk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1914688 Rfk

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutant embryos die in utero before 7.5 dpc.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941491 – 155Riboflavin kinaseAdd BLAST155

Proteomic databases

EPDiQ8CFV9
MaxQBiQ8CFV9
PaxDbiQ8CFV9
PeptideAtlasiQ8CFV9
PRIDEiQ8CFV9

PTM databases

PhosphoSitePlusiQ8CFV9

Expressioni

Gene expression databases

BgeeiENSMUSG00000024712
CleanExiMM_RFK
GenevisibleiQ8CFV9 MM

Interactioni

Subunit structurei

Monomer (By similarity). Directly interacts with TNFRSF1A death domain; this interaction may be supported by TRADD. In the absence of TNFRSF1A, interacts with TRADD. Independently of TNFRSF1A, interacts with the NADPH oxidase subunit CYBA.By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-60455N
IntActiQ8CFV9, 3 interactors
STRINGi10090.ENSMUSP00000025617

Structurei

3D structure databases

ProteinModelPortaliQ8CFV9
SMRiQ8CFV9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiKOG3110 Eukaryota
COG0196 LUCA
GeneTreeiENSGT00390000015537
HOGENOMiHOG000260803
HOVERGENiHBG049989
InParanoidiQ8CFV9
KOiK00861
OMAiGKDLGCP
OrthoDBiEOG091G0Z4E
PhylomeDBiQ8CFV9
TreeFamiTF313786

Family and domain databases

Gene3Di2.40.30.30, 1 hit
InterProiView protein in InterPro
IPR023468 Riboflavin_kinase
IPR015865 Riboflavin_kinase_bac/euk
IPR023465 Riboflavin_kinase_dom_sf
PANTHERiPTHR22749 PTHR22749, 1 hit
PfamiView protein in Pfam
PF01687 Flavokinase, 1 hit
SMARTiView protein in SMART
SM00904 Flavokinase, 1 hit
SUPFAMiSSF82114 SSF82114, 1 hit

Sequencei

Sequence statusi: Complete.

Q8CFV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSLPFFCRG QVVRGFGRGS KQLGIPTANF PEQVVDNLPA DVSTGIYYGW
60 70 80 90 100
ASVGSGDVHK MVVSIGWNPY YKNVKKSMET HIIHTFKEDF YGEILNVAIV
110 120 130 140 150
GYLRPEKNFD SLESLISAIQ GDIEEAKKQL DLPEHLKLKD DNFFQVSKGK

IMNGH
Length:155
Mass (Da):17,437
Last modified:October 3, 2003 - v2
Checksum:iAE6577023E3D34CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010607 mRNA Translation: BAB27057.1
AK002806 mRNA Translation: BAB22372.1
AK008352 mRNA Translation: BAB25622.1
BC033521 mRNA Translation: AAH33521.2
BC051021 mRNA Translation: AAH51021.1
AF031380 Genomic DNA Translation: AAB86494.1
AF031381 Genomic DNA Translation: AAB86495.1
CCDSiCCDS29688.1
RefSeqiNP_062310.1, NM_019437.3
UniGeneiMm.7013

Genome annotation databases

EnsembliENSMUST00000025617; ENSMUSP00000025617; ENSMUSG00000024712
GeneIDi54391
KEGGimmu:54391
UCSCiuc008gxo.2 mouse

Similar proteinsi

Entry informationi

Entry nameiRIFK_MOUSE
AccessioniPrimary (citable) accession number: Q8CFV9
Secondary accession number(s): O35471, O35472, Q9CQ95
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: October 3, 2003
Last modified: May 23, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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