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Protein

RNA helicase aquarius

Gene

Aqr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing as component of the spliceosome. Intron-binding spliceosomal protein required to link pre-mRNA splicing and snoRNP (small nucleolar ribonucleoprotein) biogenesis. Plays a key role in position-dependent assembly of intron-encoded box C/D small snoRNP, splicing being required for snoRNP assembly. May act by helping the folding of the snoRNA sequence. Binds to intron of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns (40 nucleotides upstream of the branchpoint) during the late stages of splicing. Has ATP-dependent RNA helicase activity and can unwind double-stranded RNA molecules with a 3' overhang (in vitro).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei801ATP; via carbonyl oxygenBy similarity1
Binding sitei806ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi826 – 831ATPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processmRNA processing, mRNA splicing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-6781823 Formation of TC-NER Pre-Incision Complex
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
RNA helicase aquarius (EC:3.6.4.13By similarity)
Alternative name(s):
Intron-binding protein of 160 kDaBy similarity
Gene namesi
Name:Aqr
Synonyms:Kiaa0560
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1276102 Aqr

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002523901 – 1481RNA helicase aquariusAdd BLAST1481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1055N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8CFQ3
MaxQBiQ8CFQ3
PaxDbiQ8CFQ3
PRIDEiQ8CFQ3

PTM databases

iPTMnetiQ8CFQ3
PhosphoSitePlusiQ8CFQ3

Expressioni

Developmental stagei

First detected at 8.5 dpc, when neural crest cells are visible at the lateral ridges of the neural plate. During embryogenesis, it is expressed in mesoderm, in the neural crest (and its target tissues) and in neuroepithelium.1 Publication

Inductioni

By retinoic acid (RA).1 Publication

Gene expression databases

BgeeiENSMUSG00000040383
CleanExiMM_AQR
ExpressionAtlasiQ8CFQ3 baseline and differential
GenevisibleiQ8CFQ3 MM

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE. Identified in a pentameric intron-binding (IB) complex composed of AQR, XAB2, ISY1, ZNF830 and PPIE that is incorporated into the spliceosome as a preassembled complex. The IB complex does not contain PRPF19. Within the spliceosome, interacts with SNRPA1, SF3B1, SF3B3, SF3A1 and SF3A2.By similarity

Protein-protein interaction databases

BioGridi198178, 1 interactor
IntActiQ8CFQ3, 2 interactors
MINTiQ8CFQ3
STRINGi10090.ENSMUSP00000047157

Structurei

3D structure databases

ProteinModelPortaliQ8CFQ3
SMRiQ8CFQ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 416Helical region with structural similarity to ARM repeat domainsBy similarityAdd BLAST416
Regioni417 – 1481Required for assembly of the IB complexBy similarityAdd BLAST1065

Domaini

Contains an N-terminal domain with structural similarity to ARM repeat regions; this domain functions as scaffold for protein-protein interactions, but is not required for RNA binding or for ATP-dependent RNA helicase activity.By similarity

Sequence similaritiesi

Belongs to the CWF11 family.Curated

Phylogenomic databases

eggNOGiKOG1806 Eukaryota
ENOG410XPV8 LUCA
GeneTreeiENSGT00810000125415
HOGENOMiHOG000170551
HOVERGENiHBG080430
InParanoidiQ8CFQ3
KOiK12874
OMAiCEIQGML
OrthoDBiEOG091G09WT
PhylomeDBiQ8CFQ3
TreeFamiTF105711

Family and domain databases

InterProiView protein in InterPro
IPR032174 Aquarius_N
IPR026300 CWF11_fam
IPR027417 P-loop_NTPase
PANTHERiPTHR10887:SF5 PTHR10887:SF5, 1 hit
PfamiView protein in Pfam
PF16399 Aquarius_N, 1 hit
PIRSFiPIRSF038901 AQR_cwf11, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits

Sequencei

Sequence statusi: Complete.

Q8CFQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPAQPKKI VAPTVSQINA EFVTQLACKY WAPHIKKKSP FDIKVIEEIY
60 70 80 90 100
EKEIVKSRFA IRKIMLLEFS QYLENYLWMN YSPEVSSKAY LMSICCMVNE
110 120 130 140 150
KFRENVPAWE TFKKKPDHFP FFFKCILKAA LAETDGEFSL HEQTLLLLFL
160 170 180 190 200
DHCFNSLEVD LIRSQVQQLI SLPMWMGLQP ARLELELKKT PKLRKFWNLI
210 220 230 240 250
KKNDEKMDPE AREQAYQERR FLSRLIQKFI SVLKSIPLSE PVTMDKVHYC
260 270 280 290 300
ERFIELMIDL EALLPTRRWF NTILDDSHLL VHCYLSSLVH REEDGHLFSQ
310 320 330 340 350
LLDMLKFYTG FEINDQTGNA LTENEMTTIH YDRITSLQRA AFAHFPELYD
360 370 380 390 400
FALSNVAEVD ARDSLVKFFG PLSSNTLHQV ASYLCLLPTL PKNEDTTFDK
410 420 430 440 450
EFLLELLVSR HERRISQIQQ LNQMPLYPTE KIIWDENIVP TEYYSGEGCL
460 470 480 490 500
ALPKLNLQFL TLHDYLLRNF NLFRLESTYE IRQDIEDSVS RMKPWQSEYG
510 520 530 540 550
GVVFGGWARM AQPIVAFTVV EVAKPNIGEN WPTRVRADVT INLNVRDHIK
560 570 580 590 600
DEWEGLRKHD VCFLITVRPT KPYGTKFDRR RPFIEQVGLV YVRGCEIQGM
610 620 630 640 650
LDDKGRVIED GPEPRPNLRG ESRTFRVFLD PNQYQQDMTN TIQNGAEDVY
660 670 680 690 700
DTFNVIMRRK PKENNFKAVL ETIRNLMNTD CVVPDWLHDI ILGYGDPSSA
710 720 730 740 750
HYSKMPNQIA TLDFNDTFLS IEHLKASFPG HNVKVTVSDP ALQIPPFRIT
760 770 780 790 800
FPVRSGKGKK RKDADGEEDD TEEAKTLIVE PHVIPNRGPY PYNQPKRNTI
810 820 830 840 850
QFTHTQIEAI RAGMQPGLTM VVGPPGTGKT DVAVQIISNI YHNFPEQRTL
860 870 880 890 900
IVTHSNQALN QLFEKIMALD IDERHLLRLG HGEEELETEK DFSRYGRVNY
910 920 930 940 950
VLARRIELLE EVKRLQKSLG VPGDASYTCE TAGYFFLYQV MSRWEEYMSR
960 970 980 990 1000
VKNSGTACPD AAPDAAQVAT FFPFHEYFAN APQPIFKGRS YEEDMEIAEG
1010 1020 1030 1040 1050
CFRHIKKIFT QLEEFRASEL LRSGLDRSKY LLVKEAKIIA MTCTHAALKR
1060 1070 1080 1090 1100
HDLVKLGFKY DNILMEEAAQ ILEIETFIPL LLQNPQDGFS RLKRWIMIGD
1110 1120 1130 1140 1150
HHQLPPVIKN MAFQKYSNME QSLFTRFVRV GVPTVDLDAQ GRARASLCNL
1160 1170 1180 1190 1200
YNWRYKNLGN LPHVQLLPEF STANAGLLYD FQLINVEDFQ GVGESEPNPY
1210 1220 1230 1240 1250
FYQNLGEAEY VVALFMYMCL LGYPADKISI LTTYNGQKHL IRDIINRRCG
1260 1270 1280 1290 1300
NNPLIGRPNK VTTVDRFQGQ QNDYILLSLV RTRAVGHLRD VRRLVVAMSR
1310 1320 1330 1340 1350
ARLGLYIFAR VSLFQNCFEL TPAFSQLTAR PLHLHIIPTE PFPTSRKNGE
1360 1370 1380 1390 1400
RPPHEVQVIK NMPQMANFVY NMYMHLIQTT HHYHQTFLQL PPAMVEEGEE
1410 1420 1430 1440 1450
GQSQETEMEA EEETVSAQGN LTPSPADASL SQETPAAQPD CSSQTEDTSA
1460 1470 1480
PCDIATAAEP VSAAAEAATP QDAESVPTET E
Length:1,481
Mass (Da):170,294
Last modified:October 17, 2006 - v2
Checksum:iDD0687337803C06B
GO

Sequence cautioni

The sequence AAB50008 differs from that shown. Reason: Frameshift at several positions.Curated
The sequence BAC65592 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112F → L in AAH42479 (PubMed:15489334).Curated1
Sequence conflicti875H → Y in AAH42479 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122310 mRNA Translation: BAC65592.1 Different initiation.
AK145547 mRNA Translation: BAE26500.1
AK151716 mRNA Translation: BAE30635.1
BC042479 mRNA Translation: AAH42479.1
U90333 mRNA Translation: AAB50008.1 Sequence problems.
CCDSiCCDS16565.1
RefSeqiNP_001277717.1, NM_001290788.1
NP_033832.2, NM_009702.3
UniGeneiMm.2545

Genome annotation databases

EnsembliENSMUST00000043160; ENSMUSP00000047157; ENSMUSG00000040383
GeneIDi11834
KEGGimmu:11834
UCSCiuc008lqd.4 mouse

Similar proteinsi

Entry informationi

Entry nameiAQR_MOUSE
AccessioniPrimary (citable) accession number: Q8CFQ3
Secondary accession number(s): P97871
, Q3U9N1, Q3ULE8, Q80TX8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 17, 2006
Last modified: May 23, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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