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Protein

Cell division control protein 42 homolog

Gene

Cdc42

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. Facilitates filopodia formation upon DOCK11-activation. Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups.By similarity

Enzyme regulationi

Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17GTPBy similarity8
Nucleotide bindingi57 – 61GTPBy similarity5
Nucleotide bindingi115 – 118GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: RGD
  • GTP binding Source: RGD
  • GTP-dependent protein binding Source: Ensembl
  • mitogen-activated protein kinase kinase kinase binding Source: RGD
  • Rho GDP-dissociation inhibitor binding Source: Ensembl

GO - Biological processi

Keywordsi

Biological processDifferentiation, Neurogenesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-114604 GPVI-mediated activation cascade
R-RNO-182971 EGFR downregulation
R-RNO-194840 Rho GTPase cycle
R-RNO-2029482 Regulation of actin dynamics for phagocytic cup formation
R-RNO-375170 CDO in myogenesis
R-RNO-389359 CD28 dependent Vav1 pathway
R-RNO-3928662 EPHB-mediated forward signaling
R-RNO-416482 G alpha (12/13) signalling events
R-RNO-418885 DCC mediated attractive signaling
R-RNO-4420097 VEGFA-VEGFR2 Pathway
R-RNO-5625970 RHO GTPases activate KTN1
R-RNO-5626467 RHO GTPases activate IQGAPs
R-RNO-5627123 RHO GTPases activate PAKs
R-RNO-5663213 RHO GTPases Activate WASPs and WAVEs
R-RNO-5663220 RHO GTPases Activate Formins
R-RNO-5687128 MAPK6/MAPK4 signaling
R-RNO-68877 Mitotic Prometaphase
R-RNO-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division control protein 42 homolog
Gene namesi
Name:Cdc42
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi71043 Cdc42

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3308943

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002708291 – 188Cell division control protein 42 homologAdd BLAST188
PropeptideiPRO_0000270830189 – 191Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64Phosphotyrosine; by SRCBy similarity1
Modified residuei188Cysteine methyl esterBy similarity1
Lipidationi188S-geranylgeranyl cysteineBy similarity1

Post-translational modificationi

Phosphorylated by SRC in an EGF-dependent manner, this stimulates the binding of the Rho-GDP dissociation inhibitor RhoGDI.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiQ8CFN2
PRIDEiQ8CFN2

PTM databases

iPTMnetiQ8CFN2
PhosphoSitePlusiQ8CFN2
SwissPalmiQ8CFN2

Expressioni

Gene expression databases

BgeeiENSRNOG00000013536
ExpressionAtlasiQ8CFN2 baseline and differential
GenevisibleiQ8CFN2 RN

Interactioni

Subunit structurei

Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, CDC42SE1, CDC42SE2, PARD6A, PARD6B and PARD6G (in a GTP-dependent manner). Interacts with activated CSPG4 and with BAIAP2. Interacts with DOCK11/Zizimin2; the interaction activates CDC42 by exchanging GDP for GTP. Interacts with DOCK9; the interaction activates CDC42 by exchanging GDP for GTP. Interacts with DOCK8 (via DHR-2 domain); the interaction activates CDC42 by exchanging GDP for GTP. Interacts with IQGAP1. Interacts with NET1 and ARHGAP33/TCGAP. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ. The GTP-bound form interacts with CCPG1. Interacts with USP6. Interacts with NEK6. Part of a collagen stimulated complex involved in cell migration composed of CDC42, CRK, TNK2 and BCAR1/p130cas. Interacts with ITGB1BP1. Interacts with ARHGDIA; this interaction inactivates and stabilizes CDC42. Interacts with ARHGDIB; this maintains CDC42 in the inactive, GDP-bound form. Interacts in (GTP-bound form) with FNBP1L and ABI1, but only in the presence of FNBP1L.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
BAIAP2Q9UQB82EBI-7023929,EBI-525456From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249080, 4 interactors
CORUMiQ8CFN2
ELMiQ8CFN2
IntActiQ8CFN2, 3 interactors
MINTiQ8CFN2
STRINGi10116.ENSRNOP00000030928

Structurei

3D structure databases

ProteinModelPortaliQ8CFN2
SMRiQ8CFN2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector regionSequence analysis9

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118978
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiQ8CFN2
KOiK04393
OMAiMQTLKCV
PhylomeDBiQ8CFN2
TreeFamiTF101109

Family and domain databases

CDDicd01874 Cdc42, 1 hit
InterProiView protein in InterPro
IPR037874 Cdc42
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8CFN2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPTVFDNY AVTVMIGGEP
60 70 80 90 100
YTLGLFDTAG QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPE
110 120 130 140 150
ITHHCPKTPF LLVGTQIDLR DDPSTIEKLA KNKQKPITPE TAEKLARDLK
160 170 180 190
AVKYVECSAL TQKGLKNVFD EAILAALEPP EPKKSRRCVL L
Length:191
Mass (Da):21,259
Last modified:January 9, 2007 - v2
Checksum:i51A437E22A4D8FFF
GO
Isoform 2 (identifier: Q8CFN2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     182-191: PKKSRRCVLL → TQPKRKCCIF

Show »
Length:191
Mass (Da):21,283
Checksum:i34B44F923D7D996B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti159A → P in AAF15538 (PubMed:11048641).Curated1
Sequence conflicti159A → P in AAN63806 (Ref. 2) Curated1
Sequence conflicti163K → R in AAF15538 (PubMed:11048641).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022241182 – 191PKKSRRCVLL → TQPKRKCCIF in isoform 2. 1 Publication10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF205635 mRNA Translation: AAF15538.1
AF491841 mRNA Translation: AAN63806.1
BC060535 mRNA Translation: AAH60535.1
RefSeqiNP_741991.3, NM_171994.4 [Q8CFN2-1]
XP_008762508.1, XM_008764286.2 [Q8CFN2-1]
XP_008762509.1, XM_008764287.2
UniGeneiRn.228625

Genome annotation databases

EnsembliENSRNOT00000029025; ENSRNOP00000030928; ENSRNOG00000013536 [Q8CFN2-1]
GeneIDi64465
KEGGirno:64465
UCSCiRGD:71043 rat [Q8CFN2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCDC42_RAT
AccessioniPrimary (citable) accession number: Q8CFN2
Secondary accession number(s): Q6P9Y3, Q71TW5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: January 9, 2007
Last modified: July 18, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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