UniProtKB - Q8CFG8 (CS1B_MOUSE)
Complement C1s-B subcomponent
C1sb
Functioni
C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4 (By similarity).
By similarityCatalytic activityi
- Cleavage of Arg-|-Ala bond in complement component C4 to form C4a and C4b, and Lys(or Arg)-|-Lys bond in complement component C2 to form C2a and C2b: the 'classical' pathway C3 convertase. EC:3.4.21.42
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 60 | CalciumBy similarity | 1 | |
Metal bindingi | 68 | CalciumBy similarity | 1 | |
Metal bindingi | 113 | CalciumBy similarity | 1 | |
Metal bindingi | 131 | CalciumBy similarity | 1 | |
Metal bindingi | 132 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 134 | CalciumBy similarity | 1 | |
Metal bindingi | 149 | CalciumBy similarity | 1 | |
Metal bindingi | 150 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 153 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 475 | Charge relay systemBy similarity | 1 | |
Active sitei | 529 | Charge relay systemBy similarity | 1 | |
Active sitei | 631 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- identical protein binding Source: MGI
- serine-type endopeptidase activity Source: MGI
GO - Biological processi
- complement activation, classical pathway Source: UniProtKB-KW
- innate immune response Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Complement pathway, Immunity, Innate immunity |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-166663, Initial triggering of complement R-MMU-173623, Classical antibody-mediated complement activation R-MMU-977606, Regulation of Complement cascade |
Protein family/group databases
MEROPSi | S01.210 |
Names & Taxonomyi
Protein namesi | Recommended name: Complement C1s-B subcomponent (EC:3.4.21.42)Alternative name(s): C1 esterase Complement component 1 subcomponent s-B Cleaved into the following 2 chains: |
Gene namesi | Name:C1sb Synonyms:C1s, Gm5077 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3644269, Gm5077 |
VEuPathDBi | HostDB:ENSMUSG00000079343 |
Subcellular locationi
Extracellular region or secreted
- extracellular region Source: MGI
- extracellular space Source: GO_Central
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 15 | By similarityAdd BLAST | 15 | |
ChainiPRO_0000042196 | 16 – 688 | Complement C1s-B subcomponentAdd BLAST | 673 | |
ChainiPRO_0000042197 | 16 – 437 | Complement C1s-B subcomponent heavy chainBy similarityAdd BLAST | 422 | |
ChainiPRO_0000042198 | 438 – 688 | Complement C1s-B subcomponent light chainBy similarityAdd BLAST | 251 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 65 ↔ 83 | By similarity | ||
Disulfide bondi | 135 ↔ 147 | By similarity | ||
Disulfide bondi | 143 ↔ 156 | By similarity | ||
Modified residuei | 149 | (3R)-3-hydroxyasparagineBy similarity | 1 | |
Disulfide bondi | 158 ↔ 171 | By similarity | ||
Glycosylationi | 174 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 175 ↔ 202 | By similarity | ||
Disulfide bondi | 234 ↔ 251 | By similarity | ||
Disulfide bondi | 294 ↔ 341 | By similarity | ||
Disulfide bondi | 321 ↔ 354 | By similarity | ||
Disulfide bondi | 359 ↔ 403 | By similarity | ||
Disulfide bondi | 386 ↔ 421 | By similarity | ||
Disulfide bondi | 425 ↔ 549 | Interchain (between heavy and light chains)PROSITE-ProRule annotation | ||
Disulfide bondi | 595 ↔ 618 | By similarity | ||
Disulfide bondi | 627 ↔ 659 | By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, HydroxylationProteomic databases
CPTACi | non-CPTAC-3971 |
PaxDbi | Q8CFG8 |
PeptideAtlasi | Q8CFG8 |
PRIDEi | Q8CFG8 |
PTM databases
GlyGeni | Q8CFG8, 1 site |
iPTMneti | Q8CFG8 |
PhosphoSitePlusi | Q8CFG8 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000079343, Expressed in multi-cellular organism and 58 other tissues |
ExpressionAtlasi | Q8CFG8, baseline and differential |
Interactioni
Subunit structurei
C1 is a calcium-dependent trimolecular complex of C1q, C1r and C1s in the molar ration of 1:2:2. Activated C1s is an disulfide-linked heterodimer of a heavy chain and a light chain (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
ComplexPortali | CPX-4985, Complement C1 complex, C1rb-C1sb variant |
STRINGi | 10090.ENSMUSP00000066999 |
Miscellaneous databases
RNActi | Q8CFG8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 16 – 130 | CUB 1PROSITE-ProRule annotationAdd BLAST | 115 | |
Domaini | 131 – 172 | EGF-like; calcium-bindingSequence analysisAdd BLAST | 42 | |
Domaini | 175 – 290 | CUB 2PROSITE-ProRule annotationAdd BLAST | 116 | |
Domaini | 292 – 356 | Sushi 1PROSITE-ProRule annotationAdd BLAST | 65 | |
Domaini | 357 – 423 | Sushi 2PROSITE-ProRule annotationAdd BLAST | 67 | |
Domaini | 438 – 680 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 243 |
Sequence similaritiesi
Keywords - Domaini
EGF-like domain, Repeat, Signal, SushiPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000157473 |
InParanoidi | Q8CFG8 |
OrthoDBi | 156878at2759 |
PhylomeDBi | Q8CFG8 |
Family and domain databases
CDDi | cd00033, CCP, 2 hits cd00041, CUB, 2 hits cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 2.60.120.290, 2 hits |
InterProi | View protein in InterPro IPR035708, Complement_C1s_subcomponent IPR000859, CUB_dom IPR001881, EGF-like_Ca-bd_dom IPR018097, EGF_Ca-bd_CS IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR035914, Sperma_CUB_dom_sf IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom IPR001254, Trypsin_dom IPR033116, TRYPSIN_SER |
PANTHERi | PTHR24255:SF18, PTHR24255:SF18, 1 hit |
Pfami | View protein in Pfam PF00431, CUB, 2 hits PF00084, Sushi, 2 hits PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00032, CCP, 2 hits SM00042, CUB, 2 hits SM00179, EGF_CA, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF49854, SSF49854, 2 hits SSF50494, SSF50494, 1 hit SSF57535, SSF57535, 2 hits |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS01180, CUB, 2 hits PS01187, EGF_CA, 1 hit PS50923, SUSHI, 2 hits PS50240, TRYPSIN_DOM, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MWYLVLFSLL ASFSAEPTMH GEILSPNYPQ AYPNDVVKSW DIEVPEGFGI
60 70 80 90 100
HLYFTHVDIE PSESCAYDSV QIISGGIEEG RLCGQRTSKS PNSPIIEEFQ
110 120 130 140 150
FPYNKLQVVF TSDFSIEEQF TGFAAYYTAI DVNECTDFTD VPCSHFCNNF
160 170 180 190 200
IGGYFCSCPP EYFLHDDMRN CGVNCSGDVF TALIGEISSP NYPNPYPENS
210 220 230 240 250
RCEYQIQLQE GFQVVVTMQR EDFDVEPADS EGNCPDSLTF AAKNQQFGPY
260 270 280 290 300
CGDGFPGPLT IRTQSNTLGI VFQTDLMGQK KGWKLRYHGD PISCPKESTA
310 320 330 340 350
NSNWEPDKAK YVFKDVVKIT CVDGFEVVEG HVSSTSYYST CQSDGQWSNS
360 370 380 390 400
GLKCQPVYCG IPDPIANGKV EEPENSVFGT VIHYTCEEPY YYMEHEEGGE
410 420 430 440 450
YRCAANGRWV NDQLGIELPR CIPVCGVPTE PFQVQQKIFG GQPAKIENFP
460 470 480 490 500
WQVFFNHPTA GGALINEYWV LTAAHVVEKN SDPSMYAGIT ALRLADLENA
510 520 530 540 550
QRLYTKRVII HPGWKEDDDL NPRTNFDNDI ALVQLKDPVK MGPKFSPICL
560 570 580 590 600
PGTSSEYNLS PGDMGLISGW GRTEKRLHVI NLRGAKVPVT SLETCKQVKE
610 620 630 640 650
ENPTARPEDY VITDNMICAG EKGVDSCKGD SGGAFAFQVP NVKAPKFYVA
660 670 680
GLVSWGKKCG AYGVYTKVKN YVDWILKTMQ ENSGPRKD
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1W2P7F1 | A0A1W2P7F1_MOUSE | Complement component 1, s subcompon... | C1s2 | 694 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF459020 mRNA Translation: AAO15559.1 |
RefSeqi | NP_776289.2, NM_173864.2 |
Genome annotation databases
Ensembli | ENSMUST00000068797; ENSMUSP00000066999; ENSMUSG00000079343 |
GeneIDi | 317677 |
KEGGi | mmu:317677 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF459020 mRNA Translation: AAO15559.1 |
RefSeqi | NP_776289.2, NM_173864.2 |
3D structure databases
SMRi | Q8CFG8 |
ModBasei | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-4985, Complement C1 complex, C1rb-C1sb variant |
STRINGi | 10090.ENSMUSP00000066999 |
Protein family/group databases
MEROPSi | S01.210 |
PTM databases
GlyGeni | Q8CFG8, 1 site |
iPTMneti | Q8CFG8 |
PhosphoSitePlusi | Q8CFG8 |
Proteomic databases
CPTACi | non-CPTAC-3971 |
PaxDbi | Q8CFG8 |
PeptideAtlasi | Q8CFG8 |
PRIDEi | Q8CFG8 |
Protocols and materials databases
DNASUi | 317677 |
Genome annotation databases
Ensembli | ENSMUST00000068797; ENSMUSP00000066999; ENSMUSG00000079343 |
GeneIDi | 317677 |
KEGGi | mmu:317677 |
Organism-specific databases
CTDi | 317677 |
MGIi | MGI:3644269, Gm5077 |
VEuPathDBi | HostDB:ENSMUSG00000079343 |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000157473 |
InParanoidi | Q8CFG8 |
OrthoDBi | 156878at2759 |
PhylomeDBi | Q8CFG8 |
Enzyme and pathway databases
Reactomei | R-MMU-166663, Initial triggering of complement R-MMU-173623, Classical antibody-mediated complement activation R-MMU-977606, Regulation of Complement cascade |
Miscellaneous databases
BioGRID-ORCSi | 317677, 0 hits in 64 CRISPR screens |
ChiTaRSi | C1s2, mouse |
PROi | PR:Q8CFG8 |
RNActi | Q8CFG8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000079343, Expressed in multi-cellular organism and 58 other tissues |
ExpressionAtlasi | Q8CFG8, baseline and differential |
Family and domain databases
CDDi | cd00033, CCP, 2 hits cd00041, CUB, 2 hits cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 2.60.120.290, 2 hits |
InterProi | View protein in InterPro IPR035708, Complement_C1s_subcomponent IPR000859, CUB_dom IPR001881, EGF-like_Ca-bd_dom IPR018097, EGF_Ca-bd_CS IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR035914, Sperma_CUB_dom_sf IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom IPR001254, Trypsin_dom IPR033116, TRYPSIN_SER |
PANTHERi | PTHR24255:SF18, PTHR24255:SF18, 1 hit |
Pfami | View protein in Pfam PF00431, CUB, 2 hits PF00084, Sushi, 2 hits PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00032, CCP, 2 hits SM00042, CUB, 2 hits SM00179, EGF_CA, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF49854, SSF49854, 2 hits SSF50494, SSF50494, 1 hit SSF57535, SSF57535, 2 hits |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 1 hit PS01180, CUB, 2 hits PS01187, EGF_CA, 1 hit PS50923, SUSHI, 2 hits PS50240, TRYPSIN_DOM, 1 hit PS00135, TRYPSIN_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CS1B_MOUSE | |
Accessioni | Q8CFG8Primary (citable) accession number: Q8CFG8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2005 |
Last sequence update: | March 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families