UniProtKB - Q8CFB4 (GBP5_MOUSE)
Guanylate-binding protein 5
Gbp5
Functioni
Catalytic activityi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 45 – 52 | GTPBy similarity | 8 | |
Nucleotide bindingi | 67 – 69 | GTPBy similarity | 3 | |
Nucleotide bindingi | 97 – 101 | GTPBy similarity | 5 |
GO - Molecular functioni
- GTPase activity Source: GO_Central
- GTP binding Source: GO_Central
- identical protein binding Source: MGI
- protein homodimerization activity Source: MGI
GO - Biological processi
- cellular response to interferon-gamma Source: MGI
- inflammatory response Source: UniProtKB-KW
- interleukin-18 production Source: UniProtKB
- interleukin-1 beta production Source: UniProtKB
- positive regulation of cytokine production involved in inflammatory response Source: UniProtKB
- positive regulation of innate immune response Source: UniProtKB
- positive regulation of NLRP3 inflammasome complex assembly Source: UniProtKB
- protein homotetramerization Source: UniProtKB
- protein localization to Golgi apparatus Source: MGI
- response to bacterium Source: MGI
Keywordsi
Molecular function | Antimicrobial, Hydrolase |
Biological process | Immunity, Inflammatory response |
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Guanylate-binding protein 5 (EC:3.6.5.-By similarity)Alternative name(s): GTP-binding protein 5 Short name: GBP-5 Short name: MuGBP-5 Guanine nucleotide-binding protein 5 |
Gene namesi | Name:Gbp5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2429943, Gbp5 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Lipid-anchor By similarity
Other locations
- Cytoplasm By similarity
Golgi apparatus
- Golgi apparatus Source: MGI
- Golgi membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: MGI
- cytoplasmic vesicle Source: MGI
- perinuclear region of cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000190970 | 1 – 587 | Guanylate-binding protein 5Add BLAST | 587 | |
PropeptideiPRO_0000370786 | 588 – 590 | Removed in mature formSequence analysis | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 587 | Cysteine methyl esterSequence analysis | 1 | |
Lipidationi | 587 | S-geranylgeranyl cysteineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Methylation, PrenylationProteomic databases
EPDi | Q8CFB4 |
PaxDbi | Q8CFB4 |
PRIDEi | Q8CFB4 |
PTM databases
iPTMneti | Q8CFB4 |
PhosphoSitePlusi | Q8CFB4 |
SwissPalmi | Q8CFB4 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000105504, Expressed in spleen and 48 other tissues |
Interactioni
Subunit structurei
Homodimer. Heterodimer with other family members, including GBP1, GBP2, GBP3 and GBP4 (By similarity). Dimerization is nucleotide-dependent (By similarity). Dimerization regulates subcellular location. May also form tetramers (dimer of dimers) in the presence of GTP (By similarity).
Interacts with NLRP3, possibly in its tetrameric form, and promotes PYCARD/ASC polymerization (PubMed:22461501).
By similarity1 PublicationGO - Molecular functioni
- identical protein binding Source: MGI
- protein homodimerization activity Source: MGI
Miscellaneous databases
RNActi | Q8CFB4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 277 | GB1/RHD3-type GPROSITE-ProRule annotationAdd BLAST | 243 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 310 | GTPase domain (Globular)By similarityAdd BLAST | 310 | |
Regioni | 1 – 306 | NLRP3-bindingBy similarityAdd BLAST | 306 | |
Regioni | 529 – 590 | Required for tetramerization, but not for dimerizationBy similarityAdd BLAST | 62 |
Sequence similaritiesi
Phylogenomic databases
GeneTreei | ENSGT00940000162684 |
HOGENOMi | CLU_018608_2_2_1 |
InParanoidi | Q8CFB4 |
OMAi | ICPDLVW |
TreeFami | TF331602 |
Family and domain databases
CDDi | cd16269, GBP_C, 1 hit |
InterProi | View protein in InterPro IPR030386, G_GB1_RHD3_dom IPR037684, GBP_C IPR003191, Guanylate-bd/ATL_C IPR036543, Guanylate-bd_C_sf IPR015894, Guanylate-bd_N IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF02263, GBP, 1 hit PF02841, GBP_C, 1 hit |
SUPFAMi | SSF48340, SSF48340, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51715, G_GB1_RHD3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPEIHMPEP LCLIGSTEGH LVTNQEALKI LSAITQPVVV VAIVGLYRTG
60 70 80 90 100
KSYLMNKLAG KEKGFSVGST VQSHTKGIWM WCVPHPQKPD HTLVLLDTEG
110 120 130 140 150
LGDVEKDDKK NDTQIFALAI LLSSTFVYNT MNKIDQGAID LLHNVTELTD
160 170 180 190 200
LLRTRNSSDS NQTEGEGPAD MSFFPDLVWT LRDFFLDLQA NGHAITSDEY
210 220 230 240 250
LENSLKLKQG SDERTQTFNL PRLCIQKFFP VKKCFVFDAP ALGSKLSQLP
260 270 280 290 300
TLSNEELNSD FVQDLSEFCS HIFTQSKTKT LPGGIQVNGP RLESLVLTYV
310 320 330 340 350
DAINSGALPS IENTVVTLAR RENSAAVQKA IGHYDQLMSE KVQLPTETLQ
360 370 380 390 400
ELLDLHRTCE REAIEIFRKH SFKDEGEFFQ KELESLLSAK QDEICKKNAD
410 420 430 440 450
ASAALCSTLL GSIFKPLEQE VAQEFYHKPG GHKLFLQRME QLKANYRQQP
460 470 480 490 500
GKGTQAEEVL QTYLNAKETV SRTILQTDQV LTDKEIQSKA EQERAEAARL
510 520 530 540 550
EAQRLEAIRI QEEQRKAEME RQHQEQLRQI ALEKARVAQE QQWILKQRAQ
560 570 580 590
EEADRIKAEQ EAQLRALQQQ LQHMREMNHH RRHHHDCVIS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ8BMN7 | Q8BMN7_MOUSE | Guanylate-binding protein 5 | Gbp5 | 724 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 488 – 490 | SKA → T in AAN46362 (Ref. 2) Curated | 3 | |
Sequence conflicti | 488 | S → K in AAN31451 (PubMed:12396730).Curated | 1 | |
Sequence conflicti | 488 | S → K in AAN52282 (PubMed:12396730).Curated | 1 | |
Sequence conflicti | 488 | S → K in AAH58555 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 501 | E → K in AAN31451 (PubMed:12396730).Curated | 1 | |
Sequence conflicti | 501 | E → K in AAN52282 (PubMed:12396730).Curated | 1 | |
Sequence conflicti | 501 | E → K in AAH58555 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_057889 | 64 – 175 | Missing in isoform 2. Add BLAST | 112 | |
Alternative sequenceiVSP_057890 | 551 – 590 | EEADR…DCVIS → GRCPVIWCLDLLLAEDEGPK QDLSQKLCCFGQEGGRLSGA EDGAASEALWISPFPETAGL CIPHCHPSNLPSVESRSQGG SRRGLRHKPLRPGGPLCTHQ EGARLSGAEDGTASEALWLS PVLETVGLFIPHPHPCSLPS TDSRSEGGSRRGLRQKPLGL VDPCALTRKVAGCL in isoform 2. Add BLAST | 40 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF422243 mRNA Translation: AAN31451.1 AF487898 mRNA Translation: AAN52282.1 AY128412 Genomic DNA Translation: AAN46362.1 AC102108 Genomic DNA No translation available. BC058555 mRNA Translation: AAH58555.1 |
CCDSi | CCDS17877.1 [Q8CFB4-1] |
RefSeqi | NP_705792.2, NM_153564.2 [Q8CFB4-1] |
Genome annotation databases
Ensembli | ENSMUST00000090127; ENSMUSP00000087587; ENSMUSG00000105504 [Q8CFB4-1] ENSMUST00000197459; ENSMUSP00000142938; ENSMUSG00000105504 [Q8CFB4-2] |
GeneIDi | 229898 |
KEGGi | mmu:229898 |
UCSCi | uc008rop.1, mouse [Q8CFB4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF422243 mRNA Translation: AAN31451.1 AF487898 mRNA Translation: AAN52282.1 AY128412 Genomic DNA Translation: AAN46362.1 AC102108 Genomic DNA No translation available. BC058555 mRNA Translation: AAH58555.1 |
CCDSi | CCDS17877.1 [Q8CFB4-1] |
RefSeqi | NP_705792.2, NM_153564.2 [Q8CFB4-1] |
3D structure databases
SMRi | Q8CFB4 |
ModBasei | Search... |
PTM databases
iPTMneti | Q8CFB4 |
PhosphoSitePlusi | Q8CFB4 |
SwissPalmi | Q8CFB4 |
Proteomic databases
EPDi | Q8CFB4 |
PaxDbi | Q8CFB4 |
PRIDEi | Q8CFB4 |
Protocols and materials databases
Antibodypediai | 33615, 302 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000090127; ENSMUSP00000087587; ENSMUSG00000105504 [Q8CFB4-1] ENSMUST00000197459; ENSMUSP00000142938; ENSMUSG00000105504 [Q8CFB4-2] |
GeneIDi | 229898 |
KEGGi | mmu:229898 |
UCSCi | uc008rop.1, mouse [Q8CFB4-1] |
Organism-specific databases
CTDi | 115362 |
MGIi | MGI:2429943, Gbp5 |
Phylogenomic databases
GeneTreei | ENSGT00940000162684 |
HOGENOMi | CLU_018608_2_2_1 |
InParanoidi | Q8CFB4 |
OMAi | ICPDLVW |
TreeFami | TF331602 |
Miscellaneous databases
BioGRID-ORCSi | 229898, 0 hits in 17 CRISPR screens |
ChiTaRSi | Gbp5, mouse |
PROi | PR:Q8CFB4 |
RNActi | Q8CFB4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000105504, Expressed in spleen and 48 other tissues |
Family and domain databases
CDDi | cd16269, GBP_C, 1 hit |
InterProi | View protein in InterPro IPR030386, G_GB1_RHD3_dom IPR037684, GBP_C IPR003191, Guanylate-bd/ATL_C IPR036543, Guanylate-bd_C_sf IPR015894, Guanylate-bd_N IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF02263, GBP, 1 hit PF02841, GBP_C, 1 hit |
SUPFAMi | SSF48340, SSF48340, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51715, G_GB1_RHD3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GBP5_MOUSE | |
Accessioni | Q8CFB4Primary (citable) accession number: Q8CFB4 Secondary accession number(s): E9QJR4, Q8CFA4, Q8CFA8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 24, 2003 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families