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Protein

Transcription factor MafA

Gene

Mafa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional factor that activates insulin gene expression (PubMed:12368292, PubMed:15665000). Acts synergistically with NEUROD1/BETA2 and PDX1 (PubMed:15665000). Binds the insulin enhancer C1/RIPE3b element (PubMed:12917329, PubMed:14680841, PubMed:14973194, PubMed:15665000). Binds to consensus TRE-type MARE 5'-TGCTGACTCAGCA-3' DNA sequence (By similarity).By similarity5 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor MafA
Alternative name(s):
Pancreatic beta-cell-specific transcriptional activator
V-maf musculoaponeurotic fibrosarcoma oncogene homolog A
Gene namesi
Name:Mafa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2673307 Mafa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian rate and survive until adulthood. They show a normal pancreatic morphology at birth. A 12 weeks, they exhibit a reduction in the proportion of Langerhans islet beta-cells and impaired glucose-stimulated insulin secretion and eventually they develop diabetes mellitus.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003202751 – 359Transcription factor MafAAdd BLAST359

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineBy similarity1
Cross-linki32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei49PhosphoserineBy similarity1
Modified residuei53PhosphothreonineBy similarity1
Modified residuei57PhosphothreonineBy similarity1
Modified residuei61PhosphoserineBy similarity1
Modified residuei65PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated, leading to its degradation by the proteasome.By similarity
Phosphorylated at tyrosines.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ8CF90
PaxDbiQ8CF90
PRIDEiQ8CF90

PTM databases

iPTMnetiQ8CF90
PhosphoSitePlusiQ8CF90

Expressioni

Tissue specificityi

Expressed in brain, lung, spleen, pancreas and kidney (PubMed:12368292, PubMed:14680841). In the pancreas, expressed in the insulin-producing beta-cells of the islets of Langerhans (at protein level) (PubMed:12917329, PubMed:15923615). Also expressed in the eye (PubMed:12368292, PubMed:15923615).4 Publications

Developmental stagei

In the developing pancreas, expressed exclusively in the insulin-positive cells from 13.5 dpc onward and never in the glucagon-expressing cells (at protein level) (PubMed:14973194). At 12.5dpc, at the mRNA level, detected in each formed somite, in myotomal cells. Also detected in the head neural tube, liver cells and, at low levels, in some mesenchyme-like cells (PubMed:14680841).2 Publications

Inductioni

Up-regulated by glucose in pancreatic beta-cell lines.1 Publication

Gene expression databases

BgeeiENSMUSG00000047591 Expressed in 21 organ(s), highest expression level in brain

Interactioni

Subunit structurei

Forms homodimers (By similarity). Interacts with NEUROD1 and PDX1 (PubMed:15665000). May interact with MAFB, FOS, JUN and PCAF (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi237545, 12 interactors
STRINGi10090.ENSMUSP00000054226

Structurei

3D structure databases

ProteinModelPortaliQ8CF90
SMRiQ8CF90
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini260 – 323bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni260 – 285Basic motifAdd BLAST26
Regioni288 – 309Leucine-zipperAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi74 – 83Poly-Gly10
Compositional biasi145 – 226His-richAdd BLAST82

Sequence similaritiesi

Belongs to the bZIP family. Maf subfamily.Curated

Phylogenomic databases

eggNOGiENOG410KDH9 Eukaryota
ENOG4112BJ1 LUCA
GeneTreeiENSGT00550000074549
HOGENOMiHOG000261683
HOVERGENiHBG000313
InParanoidiQ8CF90
KOiK07595
OMAiQRHILES
OrthoDBiEOG091G0H46
PhylomeDBiQ8CF90
TreeFamiTF325689

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR004826 bZIP_Maf
IPR013592 Maf_TF_N
IPR028562 MafA
IPR008917 TF_DNA-bd_sf
IPR024874 Transciption_factor_Maf_fam
PANTHERiPTHR10129 PTHR10129, 1 hit
PTHR10129:SF30 PTHR10129:SF30, 1 hit
PfamiView protein in Pfam
PF03131 bZIP_Maf, 1 hit
PF08383 Maf_N, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
SUPFAMiSSF47454 SSF47454, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit

Sequencei

Sequence statusi: Complete.

Q8CF90-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAELAMGAE LPSSPLAIEY VNDFDLMKFE VKKEPPEAER FCHRLPPGSL
60 70 80 90 100
SSTPLSTPCS SVPSSPSFCA PSPGTGGGAG GGGSAAQAGG APGPPSGGPG
110 120 130 140 150
TVGGASGKAV LEDLYWMSGY QHHLNPEALN LTPEDAVEAL IGSGHHGAHH
160 170 180 190 200
GAHHPAAAAA YEAFRGQSFA GGGGADDMGA GHHHGAHHTA HHHHSAHHHH
210 220 230 240 250
HHHHHHGGSG HHGGGAGHGG GGAGHHVRLE ERFSDDQLVS MSVRELNRQL
260 270 280 290 300
RGFSKEEVIR LKQKRRTLKN RGYAQSCRFK RVQQRHILES EKCQLQSQVE
310 320 330 340 350
QLKLEVGRLA KERDLYKEKY EKLAGRGGPG GAGGAGFPRE PSPAQAGPGA

AKGAPDFFL
Length:359
Mass (Da):37,576
Last modified:March 1, 2003 - v1
Checksum:iB10BC396218AB8A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB086961 Genomic DNA Translation: BAC20390.1
CCDSiCCDS27549.1
RefSeqiNP_919331.1, NM_194350.1
UniGeneiMm.309589

Genome annotation databases

EnsembliENSMUST00000062002; ENSMUSP00000054226; ENSMUSG00000047591
GeneIDi378435
KEGGimmu:378435
UCSCiuc007whe.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB086961 Genomic DNA Translation: BAC20390.1
CCDSiCCDS27549.1
RefSeqiNP_919331.1, NM_194350.1
UniGeneiMm.309589

3D structure databases

ProteinModelPortaliQ8CF90
SMRiQ8CF90
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi237545, 12 interactors
STRINGi10090.ENSMUSP00000054226

PTM databases

iPTMnetiQ8CF90
PhosphoSitePlusiQ8CF90

Proteomic databases

MaxQBiQ8CF90
PaxDbiQ8CF90
PRIDEiQ8CF90

Protocols and materials databases

DNASUi378435
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000062002; ENSMUSP00000054226; ENSMUSG00000047591
GeneIDi378435
KEGGimmu:378435
UCSCiuc007whe.1 mouse

Organism-specific databases

CTDi389692
MGIiMGI:2673307 Mafa

Phylogenomic databases

eggNOGiENOG410KDH9 Eukaryota
ENOG4112BJ1 LUCA
GeneTreeiENSGT00550000074549
HOGENOMiHOG000261683
HOVERGENiHBG000313
InParanoidiQ8CF90
KOiK07595
OMAiQRHILES
OrthoDBiEOG091G0H46
PhylomeDBiQ8CF90
TreeFamiTF325689

Miscellaneous databases

PROiPR:Q8CF90
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047591 Expressed in 21 organ(s), highest expression level in brain

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR004826 bZIP_Maf
IPR013592 Maf_TF_N
IPR028562 MafA
IPR008917 TF_DNA-bd_sf
IPR024874 Transciption_factor_Maf_fam
PANTHERiPTHR10129 PTHR10129, 1 hit
PTHR10129:SF30 PTHR10129:SF30, 1 hit
PfamiView protein in Pfam
PF03131 bZIP_Maf, 1 hit
PF08383 Maf_N, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
SUPFAMiSSF47454 SSF47454, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMAFA_MOUSE
AccessioniPrimary (citable) accession number: Q8CF90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 1, 2003
Last modified: October 10, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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