UniProtKB - Q8CF66 (LTOR4_MOUSE)
Ragulator complex protein LAMTOR4
Lamtor4
Functioni
As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated (By similarity).
By similarityGO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: Ensembl
- molecular adaptor activity Source: Ensembl
GO - Biological processi
- cellular response to amino acid stimulus Source: UniProtKB
- positive regulation of TOR signaling Source: UniProtKB
- protein localization to lysosome Source: UniProtKB
- regulation of cell size Source: UniProtKB
Enzyme and pathway databases
Reactomei | R-MMU-1632852, Macroautophagy R-MMU-165159, MTOR signalling R-MMU-166208, mTORC1-mediated signalling R-MMU-380972, Energy dependent regulation of mTOR by LKB1-AMPK R-MMU-5628897, TP53 Regulates Metabolic Genes R-MMU-8943724, Regulation of PTEN gene transcription R-MMU-9639288, Amino acids regulate mTORC1 |
Names & Taxonomyi
Protein namesi | Recommended name: Ragulator complex protein LAMTOR4Alternative name(s): Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4 Cleaved into the following chain: |
Gene namesi | Name:Lamtor4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913346, Lamtor4 |
VEuPathDBi | HostDB:ENSMUSG00000050552 |
Subcellular locationi
Lysosome
- Lysosome By similarity
Endosome
- Ragulator complex Source: UniProtKB
Lysosome
- lysosome Source: UniProtKB
Other locations
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
LysosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000424499 | 1 – 99 | Ragulator complex protein LAMTOR4Add BLAST | 99 | |
Initiator methioninei | Removed; alternateBy similarity | |||
ChainiPRO_0000325842 | 2 – 99 | Ragulator complex protein LAMTOR4, N-terminally processedAdd BLAST | 98 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 2 | N-acetylthreonine; in Ragulator complex protein LAMTOR4, N-terminally processedBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q8CF66 |
jPOSTi | Q8CF66 |
MaxQBi | Q8CF66 |
PaxDbi | Q8CF66 |
PRIDEi | Q8CF66 |
ProteomicsDBi | 292051 |
TopDownProteomicsi | Q8CF66 |
PTM databases
PhosphoSitePlusi | Q8CF66 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000050552, Expressed in granulocyte and 303 other tissues |
Genevisiblei | Q8CF66, MM |
Interactioni
Subunit structurei
Part of the Ragulator complex composed of LAMTOR1, LAMTOR2, LAMTOR3, LAMTOR4 and LAMTOR5. LAMTOR4 and LAMTOR5 form a heterodimer that interacts, through LAMTOR1, with a LAMTOR2, LAMTOR3 heterodimer. The Ragulator complex interacts with both the mTORC1 complex and heterodimers constituted of the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD; regulated by amino acid availability. The Ragulator complex interacts with SLC38A9; the probable amino acid sensor.
Component of the lysosomal folliculin complex (LFC), composed of FLCN, FNIP1 (or FNIP2), RRAGA/RagA or RRAGB/RagB GDP-bound, RRAGC/RagC or RRAGD/RagD GTP-bound, and Ragulator.
By similarityProtein-protein interaction databases
BioGRIDi | 211211, 7 interactors |
ComplexPortali | CPX-4761, Ragulator complex |
CORUMi | Q8CF66 |
IntActi | Q8CF66, 1 interactor |
STRINGi | 10090.ENSMUSP00000056693 |
Miscellaneous databases
RNActi | Q8CF66, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502S3B2, Eukaryota |
GeneTreei | ENSGT00390000016053 |
HOGENOMi | CLU_137556_1_0_1 |
InParanoidi | Q8CF66 |
OMAi | DESFMPN |
OrthoDBi | 1589714at2759 |
PhylomeDBi | Q8CF66 |
TreeFami | TF324352 |
Family and domain databases
InterProi | View protein in InterPro IPR034601, LAMTOR4 |
PANTHERi | PTHR33967, PTHR33967, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MTSALTQGLE RIPDQLGYLV LSEGAVLASS GDLENDEQAA SAISELVSTA
60 70 80 90
CGFQLHHGTN IPFKRLSVVF GEHTLLVTVS GQRVFVVKRQ NRGREPIDV
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 81 | G → R in BAC24993 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK002488 mRNA Translation: BAC24993.1 Different initiation. AK003086 mRNA Translation: BAC25020.1 Different initiation. AK010598 mRNA Translation: BAC25306.1 Different initiation. BC144958 mRNA Translation: AAI44959.1 |
CCDSi | CCDS39342.1 |
RefSeqi | NP_001074577.1, NM_001081108.2 |
Genome annotation databases
Ensembli | ENSMUST00000062067; ENSMUSP00000056693; ENSMUSG00000050552 |
GeneIDi | 66096 |
KEGGi | mmu:66096 |
UCSCi | uc009afd.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK002488 mRNA Translation: BAC24993.1 Different initiation. AK003086 mRNA Translation: BAC25020.1 Different initiation. AK010598 mRNA Translation: BAC25306.1 Different initiation. BC144958 mRNA Translation: AAI44959.1 |
CCDSi | CCDS39342.1 |
RefSeqi | NP_001074577.1, NM_001081108.2 |
3D structure databases
SMRi | Q8CF66 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 211211, 7 interactors |
ComplexPortali | CPX-4761, Ragulator complex |
CORUMi | Q8CF66 |
IntActi | Q8CF66, 1 interactor |
STRINGi | 10090.ENSMUSP00000056693 |
PTM databases
PhosphoSitePlusi | Q8CF66 |
Proteomic databases
EPDi | Q8CF66 |
jPOSTi | Q8CF66 |
MaxQBi | Q8CF66 |
PaxDbi | Q8CF66 |
PRIDEi | Q8CF66 |
ProteomicsDBi | 292051 |
TopDownProteomicsi | Q8CF66 |
Protocols and materials databases
Antibodypediai | 16403, 64 antibodies from 16 providers |
Genome annotation databases
Ensembli | ENSMUST00000062067; ENSMUSP00000056693; ENSMUSG00000050552 |
GeneIDi | 66096 |
KEGGi | mmu:66096 |
UCSCi | uc009afd.2, mouse |
Organism-specific databases
CTDi | 389541 |
MGIi | MGI:1913346, Lamtor4 |
VEuPathDBi | HostDB:ENSMUSG00000050552 |
Phylogenomic databases
eggNOGi | ENOG502S3B2, Eukaryota |
GeneTreei | ENSGT00390000016053 |
HOGENOMi | CLU_137556_1_0_1 |
InParanoidi | Q8CF66 |
OMAi | DESFMPN |
OrthoDBi | 1589714at2759 |
PhylomeDBi | Q8CF66 |
TreeFami | TF324352 |
Enzyme and pathway databases
Reactomei | R-MMU-1632852, Macroautophagy R-MMU-165159, MTOR signalling R-MMU-166208, mTORC1-mediated signalling R-MMU-380972, Energy dependent regulation of mTOR by LKB1-AMPK R-MMU-5628897, TP53 Regulates Metabolic Genes R-MMU-8943724, Regulation of PTEN gene transcription R-MMU-9639288, Amino acids regulate mTORC1 |
Miscellaneous databases
BioGRID-ORCSi | 66096, 17 hits in 65 CRISPR screens |
ChiTaRSi | Lamtor4, mouse |
PROi | PR:Q8CF66 |
RNActi | Q8CF66, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000050552, Expressed in granulocyte and 303 other tissues |
Genevisiblei | Q8CF66, MM |
Family and domain databases
InterProi | View protein in InterPro IPR034601, LAMTOR4 |
PANTHERi | PTHR33967, PTHR33967, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LTOR4_MOUSE | |
Accessioni | Q8CF66Primary (citable) accession number: Q8CF66 Secondary accession number(s): B7ZN18, Q8CEW4, Q8CF77 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | March 18, 2008 | |
Last modified: | February 23, 2022 | |
This is version 106 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families