UniProtKB - Q8CE96 (TRM6_MOUSE)
Protein
tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
Gene
Trmt6
Organism
Mus musculus (Mouse)
Status
Functioni
Substrate-binding subunit of tRNA (adenine-N1-)-methyltransferase, which catalyzes the formation of N1-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Together with the TRMT61A catalytic subunit, part of a mRNA N1-methyltransferase complex that mediates methylation of adenosine residues at the N1 position of a small subset of mRNAs: N1 methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 349 | SubstrateBy similarity | 1 | |
Binding sitei | 377 | SubstrateBy similarity | 1 |
GO - Biological processi
- mRNA methylation Source: UniProtKB
- tRNA methylation Source: InterPro
Keywordsi
Biological process | tRNA processing |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6Alternative name(s): mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6By similarity tRNA(m1A58)-methyltransferase subunit TRM6 Short name: tRNA(m1A58)MTase subunit TRM6 |
Gene namesi | Name:Trmt6 Synonyms:Kiaa1153, Trm6 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914176, Trmt6 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleus Source: GO_Central
Other locations
- tRNA (m1A) methyltransferase complex Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000233099 | 1 – 497 | tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6Add BLAST | 497 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 108 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8CE96 |
PaxDbi | Q8CE96 |
PeptideAtlasi | Q8CE96 |
PRIDEi | Q8CE96 |
PTM databases
iPTMneti | Q8CE96 |
PhosphoSitePlusi | Q8CE96 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000037376, Expressed in blastocyst and 255 other tissues |
Genevisiblei | Q8CE96, MM |
Interactioni
Subunit structurei
Heterotetramer; composed of two copies of TRMT6 and two copies of TRMT61A.
By similarityProtein-protein interaction databases
BioGRIDi | 211814, 10 interactors |
STRINGi | 10090.ENSMUSP00000044687 |
Miscellaneous databases
RNActi | Q8CE96, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 95 – 105 | SubstrateBy similarityAdd BLAST | 11 | |
Regioni | 146 – 155 | SubstrateBy similarity | 10 | |
Regioni | 176 – 183 | SubstrateBy similarity | 8 | |
Regioni | 415 – 423 | SubstrateBy similarity | 9 | |
Regioni | 434 – 441 | SubstrateBy similarity | 8 |
Sequence similaritiesi
Belongs to the TRM6/GCD10 family.Curated
Phylogenomic databases
eggNOGi | KOG1416, Eukaryota |
GeneTreei | ENSGT00390000008327 |
HOGENOMi | CLU_010916_0_1_1 |
InParanoidi | Q8CE96 |
OMAi | ETCSGLV |
OrthoDBi | 543764at2759 |
PhylomeDBi | Q8CE96 |
TreeFami | TF314835 |
Family and domain databases
InterProi | View protein in InterPro IPR017423, TRM6 |
PANTHERi | PTHR12945, PTHR12945, 1 hit |
Pfami | View protein in Pfam PF04189, Gcd10p, 1 hit |
PIRSFi | PIRSF038170, tRNA_m1A_mtfrase, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q8CE96-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEASAAEQPS SPPPPLGDHC IHDGDFVVLK REDVFKAVQV QRRKKVTFEK
60 70 80 90 100
QWFYLDNAIG HSYGSAFDVS SGGSLQLRKK LEEPASETKE AGTDNRNIVD
110 120 130 140 150
DGKSQKLTQD DIKALKDKGI KGEEIVQQLI ENSTTFRDKT EFAQDKYIKK
160 170 180 190 200
KKKKYEAIVT ILKPSTRILS IMYYAREPGK INHMRYDTLA QMLTLGNIRA
210 220 230 240 250
GNKMIVMETC SGLVLGAMME RMGGFGSIIQ LYPGDGPVRA ATACFGFPKS
260 270 280 290 300
FLSGLYEFPL NKVNSLLNGT FSAEMLSSEP KDSTPVEESN GELEEKEIAE
310 320 330 340 350
QADEDNIVDA AENNSGEQRP MEIVPGDPEN KEPKEKRSKR DYIQEKQRRQ
360 370 380 390 400
EEQRKRHLEA AALLGERNAD GLIVASRFHP TPLLLSLLDF VAPSRPFVVY
410 420 430 440 450
CQYKEPLLEC YTKLRERGGV INLRLSETWL RNYQVLPDRS HPKLLMSGGG
460 470 480 490
GYLLSGFTVV SDSLRADPSL KSCTGALDPH KAEEPAAKKQ KCMESAS
Sequence cautioni
The sequence BAC98110 differs from that shown. Reason: Frameshift.Curated
The sequence BAC98110 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 9 | P → Q in BAE38495 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 86 | S → P in BAE38495 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 159 | V → I in BAE38495 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 283 | S → N in BAE38495 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 377 | R → H in BAE39347 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018026 | 1 – 183 | Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST | 183 | |
Alternative sequenceiVSP_018027 | 343 – 358 | IQEKQ…RKRHL → VSMSRVIILVIASEPR in isoform 4. 1 PublicationAdd BLAST | 16 | |
Alternative sequenceiVSP_018028 | 359 – 497 | Missing in isoform 4. 1 PublicationAdd BLAST | 139 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK129300 mRNA Translation: BAC98110.1 Sequence problems. AK014359 mRNA Translation: BAC25432.1 AK165976 mRNA Translation: BAE38495.1 AK167222 mRNA Translation: BAE39347.1 AK028755 mRNA Translation: BAC26100.1 BC049083 mRNA Translation: AAH49083.1 BC052648 mRNA Translation: AAH52648.1 |
CCDSi | CCDS50728.1 [Q8CE96-1] |
RefSeqi | NP_780322.2, NM_175113.3 [Q8CE96-1] XP_006500115.1, XM_006500052.2 |
Genome annotation databases
Ensembli | ENSMUST00000039554; ENSMUSP00000044687; ENSMUSG00000037376 [Q8CE96-1] |
GeneIDi | 66926 |
KEGGi | mmu:66926 |
UCSCi | uc008mng.1, mouse [Q8CE96-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK129300 mRNA Translation: BAC98110.1 Sequence problems. AK014359 mRNA Translation: BAC25432.1 AK165976 mRNA Translation: BAE38495.1 AK167222 mRNA Translation: BAE39347.1 AK028755 mRNA Translation: BAC26100.1 BC049083 mRNA Translation: AAH49083.1 BC052648 mRNA Translation: AAH52648.1 |
CCDSi | CCDS50728.1 [Q8CE96-1] |
RefSeqi | NP_780322.2, NM_175113.3 [Q8CE96-1] XP_006500115.1, XM_006500052.2 |
3D structure databases
SMRi | Q8CE96 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 211814, 10 interactors |
STRINGi | 10090.ENSMUSP00000044687 |
PTM databases
iPTMneti | Q8CE96 |
PhosphoSitePlusi | Q8CE96 |
Proteomic databases
EPDi | Q8CE96 |
PaxDbi | Q8CE96 |
PeptideAtlasi | Q8CE96 |
PRIDEi | Q8CE96 |
Protocols and materials databases
Antibodypediai | 23943, 78 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000039554; ENSMUSP00000044687; ENSMUSG00000037376 [Q8CE96-1] |
GeneIDi | 66926 |
KEGGi | mmu:66926 |
UCSCi | uc008mng.1, mouse [Q8CE96-1] |
Organism-specific databases
CTDi | 51605 |
MGIi | MGI:1914176, Trmt6 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG1416, Eukaryota |
GeneTreei | ENSGT00390000008327 |
HOGENOMi | CLU_010916_0_1_1 |
InParanoidi | Q8CE96 |
OMAi | ETCSGLV |
OrthoDBi | 543764at2759 |
PhylomeDBi | Q8CE96 |
TreeFami | TF314835 |
Miscellaneous databases
BioGRID-ORCSi | 66926, 8 hits in 18 CRISPR screens |
ChiTaRSi | Trmt6, mouse |
PROi | PR:Q8CE96 |
RNActi | Q8CE96, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037376, Expressed in blastocyst and 255 other tissues |
Genevisiblei | Q8CE96, MM |
Family and domain databases
InterProi | View protein in InterPro IPR017423, TRM6 |
PANTHERi | PTHR12945, PTHR12945, 1 hit |
Pfami | View protein in Pfam PF04189, Gcd10p, 1 hit |
PIRSFi | PIRSF038170, tRNA_m1A_mtfrase, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRM6_MOUSE | |
Accessioni | Q8CE96Primary (citable) accession number: Q8CE96 Secondary accession number(s): Q3TJZ8 Q8CEU0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2006 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families