UniProtKB - Q8CDK2 (CBPC2_MOUSE)
Protein
Cytosolic carboxypeptidase 2
Gene
Agbl2
Organism
Mus musculus (Mouse)
Status
Functioni
Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Does not show detyrosinase or deglycylase activities from the carboxy-terminus of tubulin.1 Publication
Caution
Was originally thought to have detyrosinating activity from C-terminal positions on tubulin.By similarity
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Activity regulationi
Inhibited by RARRES1.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 425 | ZincBy similarity | 1 | |
Metal bindingi | 428 | ZincBy similarity | 1 | |
Active sitei | 475 | NucleophileBy similarity | 1 | |
Metal bindingi | 521 | ZincBy similarity | 1 |
GO - Molecular functioni
- metallocarboxypeptidase activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- protein side chain deglutamylation Source: UniProtKB
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Protein family/group databases
MEROPSi | M14.029 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic carboxypeptidase 2 (EC:3.4.17.-)Alternative name(s): ATP/GTP-binding protein-like 2 |
Gene namesi | Name:Agbl2 Synonyms:Ccp2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2443254, Agbl2 |
Subcellular locationi
Cytosol
- cytosol 1 Publication
Cytoskeleton
- centriole By similarity
- cilium basal body By similarity
Note: Colocalizes with gamma-tubulin in the centrioles and with glutamylated tubulin in the basal bodies of ciliated cells.By similarity
Cytoskeleton
- centriole Source: UniProtKB
- ciliary basal body Source: UniProtKB
Cytosol
- cytosol Source: UniProtKB
Keywords - Cellular componenti
Cell projection, Cytoplasm, CytoskeletonPathology & Biotechi
Disruption phenotypei
AGBL2 and AGBL3 double mutants are viable and display no obvious phenotypic alterations.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 593 | E → A: Abolishes deglutamylase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000283750 | 1 – 862 | Cytosolic carboxypeptidase 2Add BLAST | 862 |
Proteomic databases
PaxDbi | Q8CDK2 |
PRIDEi | Q8CDK2 |
PTM databases
iPTMneti | Q8CDK2 |
PhosphoSitePlusi | Q8CDK2 |
Expressioni
Tissue specificityi
Widely expressed. Expressed in tissues with motile cilia such as testis, lung and trachea. Also detected in brain, eye, muscle, pancreas, intestine, stomach, pituitary, spleen, adrenal and kidney. Expressed in mitral and granular cells in brain.2 Publications
Inductioni
Up-regulated during ciliogenesis.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000040812, Expressed in spermatocyte and 49 other tissues |
ExpressionAtlasi | Q8CDK2, baseline and differential |
Interactioni
Protein-protein interaction databases
IntActi | Q8CDK2, 1 interactor |
MINTi | Q8CDK2 |
STRINGi | 10090.ENSMUSP00000129216 |
Miscellaneous databases
RNActi | Q8CDK2, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase M14 family.Curated
Phylogenomic databases
eggNOGi | KOG3641, Eukaryota |
GeneTreei | ENSGT00940000160201 |
InParanoidi | Q8CDK2 |
OrthoDBi | 481670at2759 |
PhylomeDBi | Q8CDK2 |
TreeFami | TF313794 |
Family and domain databases
InterProi | View protein in InterPro IPR040626, Pepdidase_M14_N IPR000834, Peptidase_M14 |
Pfami | View protein in Pfam PF18027, Pepdidase_M14_N, 1 hit PF00246, Peptidase_M14, 1 hit |
s (7+)i Sequence
Sequence statusi: Complete.
This entry describes 7 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 7 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8CDK2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNVLLEMAFL SQTLPDPYED FIHHHLQYYG YFKAQKSSLP NSGTHQRVWR
60 70 80 90 100
NSPRYMLNGS FGERDDFISD SLEKEMLLWP TCLSSTGHAH IDAVNRDSLL
110 120 130 140 150
LSSPLLRTRQ LIFDELDEAS PRLREPRELF SCFSSRGPLQ APRWPIECEV
160 170 180 190 200
IKENIHHIEW VPHQPEYFYQ PTGSEKVPEI VGEEQGTVVY QLDSVPAEGT
210 220 230 240 250
YFTSSRIGGK RGTIKELAVT LQGPDDNTLL FESRFESGNL QKAVRVGIYE
260 270 280 290 300
YELTLRTDLY TDKHTQWFYF RVQNTRKDAT YRFTIVNLLK PKSLYAVGMK
310 320 330 340 350
PLMYSQLDAT IYNIGWRREG REIKYYKNNV DDGQQPLYCL TWTTQFPHDQ
360 370 380 390 400
DTCFFAHFYP YTYTDLQCYL LSVANNPIQS QFCKLRALCR SLAGNTVYLL
410 420 430 440 450
TITNPSRTPQ EAAAKKAVVL SARVHPGESN SSWIMNGFLD FILSNSPDAQ
460 470 480 490 500
LLRDIFVFKV IPMLNPDGVI VGNYRCSLAG RDLNRHYKTV LKDSFPCIWY
510 520 530 540 550
TKNMIKRLLE EREVLLYCDF HGHSRKNNIF LYGCHSNNRK HWLHERVFPL
560 570 580 590 600
MLSKNAPDKF SFDSCNFKVQ KCKEGTGRVV MWRMGIINSY TMESTFGGST
610 620 630 640 650
LGSKRDTHFT IEDLKSLGYH VCDTLLDFCD PDQTKYTQCL QELKELLQQE
660 670 680 690 700
INKKLNNFGQ DMDLEGNWSD IPLSDIESST SGSDSSLSDG PPIRLLNIVA
710 720 730 740 750
DEPNQKTVLK NPKKKRLQTR KQRNEQYQKS YLMRELKLTE NAPGRARFVS
760 770 780 790 800
TLQKQPTFLK SPESPSPSVR RSENPRLNET QLSGREKGTS LDPPLTSPKN
810 820 830 840 850
KERIQSKKPG FTASCSPKRS TNSSLGPAPD VKPNWSKTRY SATRKDHATM
860
AVYPSLHIYT YP
Isoform 3 (identifier: Q8CDK2-3) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
807-836: KKPGFTASCSPKRSTNSSLGPAPDVKPNWS → YSQPCVFSLILHEHQAQTSSDTPCQNPESH
837-862: Missing.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.Curated
Show »Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0B4J1L6 | A0A0B4J1L6_MOUSE | Cytosolic carboxypeptidase 2 | Agbl2 | 711 | Annotation score: |
Sequence cautioni
The sequence ABI51945 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence ABI51946 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence ABI51947 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence ABI51948 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence ABI51949 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAC37726 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence CAM20823 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 134 | S → F in BAC26827 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 146 | I → L in BAC26827 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 206 | R → T in BAC26827 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 749 | V → F in BAC26827 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_024362 | 1 – 298 | Missing in isoform 7. 1 PublicationAdd BLAST | 298 | |
Alternative sequenceiVSP_024363 | 2 – 12 | NVLLEMAFLSQ → FPALETELKPE in isoform 6. 1 PublicationAdd BLAST | 11 | |
Alternative sequenceiVSP_024364 | 79 – 97 | WPTCL…AVNRD → Y in isoform 5. 1 PublicationAdd BLAST | 19 | |
Alternative sequenceiVSP_024365 | 744 – 779 | Missing in isoform 4 and isoform 5. 1 PublicationAdd BLAST | 36 | |
Alternative sequenceiVSP_024366 | 806 – 809 | SKKP → GFST in isoform 2 and isoform 6. 2 Publications | 4 | |
Alternative sequenceiVSP_024367 | 807 – 836 | KKPGF…KPNWS → YSQPCVFSLILHEHQAQTSS DTPCQNPESH in isoform 3 and isoform 7. 2 PublicationsAdd BLAST | 30 | |
Alternative sequenceiVSP_024368 | 810 – 862 | Missing in isoform 2 and isoform 6. 2 PublicationsAdd BLAST | 53 | |
Alternative sequenceiVSP_024369 | 837 – 862 | Missing in isoform 3 and isoform 7. 2 PublicationsAdd BLAST | 26 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ867026 mRNA Translation: ABI51945.1 Different initiation. DQ867027 mRNA Translation: ABI51946.1 Different initiation. DQ867028 mRNA Translation: ABI51947.1 Different initiation. DQ867029 mRNA Translation: ABI51948.1 Different initiation. DQ867030 mRNA Translation: ABI51949.1 Different initiation. AK029744 mRNA Translation: BAC26594.1 AK029932 mRNA Translation: BAC26686.1 AK030138 mRNA Translation: BAC26802.1 AK030182 mRNA Translation: BAC26827.1 AK040259 mRNA Translation: BAC30555.2 AK079691 mRNA Translation: BAC37726.2 Different initiation. AL714026 Genomic DNA Translation: CAM20823.1 Sequence problems. AL714026 Genomic DNA Translation: CAM20824.1 AL714026 Genomic DNA Translation: CAM20825.1 |
CCDSi | CCDS38177.2 [Q8CDK2-1] CCDS89512.1 [Q8CDK2-6] |
RefSeqi | NP_848870.2, NM_178755.3 [Q8CDK2-1] XP_006499695.1, XM_006499632.2 [Q8CDK2-1] XP_006499703.1, XM_006499640.1 [Q8CDK2-4] XP_006499704.1, XM_006499641.3 XP_006499705.1, XM_006499642.3 [Q8CDK2-2] XP_006499706.1, XM_006499643.1 [Q8CDK2-5] XP_011237881.1, XM_011239579.1 [Q8CDK2-5] |
Genome annotation databases
Ensembli | ENSMUST00000037206; ENSMUSP00000047936; ENSMUSG00000040812 [Q8CDK2-6] ENSMUST00000037219; ENSMUSP00000048647; ENSMUSG00000040812 [Q8CDK2-1] ENSMUST00000051831; ENSMUSP00000051620; ENSMUSG00000040812 [Q8CDK2-3] ENSMUST00000111481; ENSMUSP00000107106; ENSMUSG00000040812 [Q8CDK2-1] ENSMUST00000170320; ENSMUSP00000129216; ENSMUSG00000040812 [Q8CDK2-1] |
GeneIDi | 271813 |
KEGGi | mmu:271813 |
UCSCi | uc008ktc.2, mouse [Q8CDK2-6] uc008ktd.2, mouse [Q8CDK2-2] uc008kte.2, mouse [Q8CDK2-1] uc008ktg.2, mouse [Q8CDK2-4] uc008kth.2, mouse [Q8CDK2-5] uc008ktk.1, mouse [Q8CDK2-7] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ867026 mRNA Translation: ABI51945.1 Different initiation. DQ867027 mRNA Translation: ABI51946.1 Different initiation. DQ867028 mRNA Translation: ABI51947.1 Different initiation. DQ867029 mRNA Translation: ABI51948.1 Different initiation. DQ867030 mRNA Translation: ABI51949.1 Different initiation. AK029744 mRNA Translation: BAC26594.1 AK029932 mRNA Translation: BAC26686.1 AK030138 mRNA Translation: BAC26802.1 AK030182 mRNA Translation: BAC26827.1 AK040259 mRNA Translation: BAC30555.2 AK079691 mRNA Translation: BAC37726.2 Different initiation. AL714026 Genomic DNA Translation: CAM20823.1 Sequence problems. AL714026 Genomic DNA Translation: CAM20824.1 AL714026 Genomic DNA Translation: CAM20825.1 |
CCDSi | CCDS38177.2 [Q8CDK2-1] CCDS89512.1 [Q8CDK2-6] |
RefSeqi | NP_848870.2, NM_178755.3 [Q8CDK2-1] XP_006499695.1, XM_006499632.2 [Q8CDK2-1] XP_006499703.1, XM_006499640.1 [Q8CDK2-4] XP_006499704.1, XM_006499641.3 XP_006499705.1, XM_006499642.3 [Q8CDK2-2] XP_006499706.1, XM_006499643.1 [Q8CDK2-5] XP_011237881.1, XM_011239579.1 [Q8CDK2-5] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q8CDK2, 1 interactor |
MINTi | Q8CDK2 |
STRINGi | 10090.ENSMUSP00000129216 |
Protein family/group databases
MEROPSi | M14.029 |
PTM databases
iPTMneti | Q8CDK2 |
PhosphoSitePlusi | Q8CDK2 |
Proteomic databases
PaxDbi | Q8CDK2 |
PRIDEi | Q8CDK2 |
Protocols and materials databases
Antibodypediai | 1166, 107 antibodies |
DNASUi | 271813 |
Genome annotation databases
Ensembli | ENSMUST00000037206; ENSMUSP00000047936; ENSMUSG00000040812 [Q8CDK2-6] ENSMUST00000037219; ENSMUSP00000048647; ENSMUSG00000040812 [Q8CDK2-1] ENSMUST00000051831; ENSMUSP00000051620; ENSMUSG00000040812 [Q8CDK2-3] ENSMUST00000111481; ENSMUSP00000107106; ENSMUSG00000040812 [Q8CDK2-1] ENSMUST00000170320; ENSMUSP00000129216; ENSMUSG00000040812 [Q8CDK2-1] |
GeneIDi | 271813 |
KEGGi | mmu:271813 |
UCSCi | uc008ktc.2, mouse [Q8CDK2-6] uc008ktd.2, mouse [Q8CDK2-2] uc008kte.2, mouse [Q8CDK2-1] uc008ktg.2, mouse [Q8CDK2-4] uc008kth.2, mouse [Q8CDK2-5] uc008ktk.1, mouse [Q8CDK2-7] |
Organism-specific databases
CTDi | 79841 |
MGIi | MGI:2443254, Agbl2 |
Phylogenomic databases
eggNOGi | KOG3641, Eukaryota |
GeneTreei | ENSGT00940000160201 |
InParanoidi | Q8CDK2 |
OrthoDBi | 481670at2759 |
PhylomeDBi | Q8CDK2 |
TreeFami | TF313794 |
Miscellaneous databases
BioGRID-ORCSi | 271813, 0 hits in 17 CRISPR screens |
ChiTaRSi | Agbl2, mouse |
PROi | PR:Q8CDK2 |
RNActi | Q8CDK2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000040812, Expressed in spermatocyte and 49 other tissues |
ExpressionAtlasi | Q8CDK2, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR040626, Pepdidase_M14_N IPR000834, Peptidase_M14 |
Pfami | View protein in Pfam PF18027, Pepdidase_M14_N, 1 hit PF00246, Peptidase_M14, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CBPC2_MOUSE | |
Accessioni | Q8CDK2Primary (citable) accession number: Q8CDK2 Secondary accession number(s): A2AH35 Q8CDQ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries