Atg14

Functionⁱ

Required for both basal and inducible autophagy (PubMed:19270696, PubMed:19270693). Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (By similarity). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:19270693). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (By similarity). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (By similarity). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation of PIK3C3 by AMPK (PubMed:23332761). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (By similarity). Modulates the hepatic lipid metabolism (PubMed:22992773).By similarity4 Publications

GO - Molecular functionⁱ

GTPase binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

autophagosome assembly Source: MGI
autophagosome membrane docking Source: MGI
autophagy
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 19270696
cellular response to glucose starvation
Source: UniProtKB
Inferred from direct assayⁱ
- 23332761
cellular response to starvation Source: MGI
endosome to lysosome transport
Source: MGI
Inferred from sequence orthologyⁱ
- 22797916
macroautophagy Source: MGI
mitophagy Source: MGI
negative regulation of protein phosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
- 23332761
positive regulation of phosphatidylinositol 3-kinase activity Source: MGI
positive regulation of protein phosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
- 23332761
posttranscriptional regulation of gene expression Source: MGI
regulation of protein phosphorylation Source: MGI
regulation of triglyceride metabolic process
Source: MGI
Inferred from mutant phenotypeⁱ
- 22992773
response to mitochondrial depolarisation Source: MGI

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Autophagy

Biological process

Autophagy

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1632852 Macroautophagy

Reactomeⁱ

R-MMU-1632852 Macroautophagy

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Beclin 1-associated autophagy-related key regulatorBy similarity Short name: BarkorBy similarity Alternative name(s): Autophagy-related protein 14-like protein1 Publication Short name: Atg14L1 Publication
Gene namesⁱ	Name:Atg14Imported Synonyms:Atg14L1 Publication, D14Ertd436e, Kiaa0831
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 14

Organism-specific databases

MGIⁱ	MGI:1261775 Atg14

Keywords - Cellular componentⁱ

Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000050775	1 – 492	Beclin 1-associated autophagy-related key regulatorAdd BLAST		492

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	29	PhosphoserineBy similarity	1
Modified residueⁱ	416	PhosphoserineBy similarity	1
Modified residueⁱ	429	PhosphothreonineBy similarity	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q8CDJ3
PaxDbⁱ	Q8CDJ3
PeptideAtlas More...PeptideAtlasⁱ	Q8CDJ3
PRIDEⁱ	Q8CDJ3

PTM databases

iPTMnetⁱ	Q8CDJ3
PhosphoSitePlusⁱ	Q8CDJ3

Expressionⁱ

Tissue specificityⁱ

Widely expressed.1 Publication

Inductionⁱ

Expression is controlled by forkhead box O FoxO1 transcription factor and circadian rhythms.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000037526 Expressed in 270 organ(s), highest expression level in morula
CleanExⁱ	MM_D14ERTD436E
Genevisibleⁱ	Q8CDJ3 MM

Interactionⁱ

Subunit structureⁱ

Forms homooligomers; homo-oligomerization is essential for the roles in membrane tethering and enhancement of SNARE-mediated fusion (By similarity). Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex I (PI3KC3-C1) in which the core composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 is associated with ATG14 (PubMed:19270693, PubMed:22745922, PubMed:23332761). PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex (By similarity). PI3KC3-C1 can associate with further regulatory subunits (PubMed:24849286). Interacts with PIK3CB (PubMed:21059846). Interacts (via coiled-coil domain) with BECN2 (via coiled-coil domain); this interaction is tighter than BECN2 self-association (By similarity). Interacts with the STX17-SNAP29 binary t-SNARE complex (By similarity). Interacts with NRBF2 (PubMed:24849286).By similarity5 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Gja1	P23242	2	EBI-3506699,EBI-298630
Nrbf2	Q8VCQ3	7	EBI-3506699,EBI-2365563

GO - Molecular functionⁱ

GTPase binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

ComplexPortalⁱ	CPX-75 Phosphatidylinositol 3-kinase complex, class III, ATG14 variant
Database of interacting proteins More...DIPⁱ	DIP-60849N
IntActⁱ	Q8CDJ3, 11 interactors
STRINGⁱ	10090.ENSMUSP00000039047

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q8CDJ3
SMRⁱ	Q8CDJ3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	413 – 492	BATSAdd BLAST		80

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	70 – 180	Sequence analysisAdd BLAST		111

Domainⁱ

The coiled-coil domain is required for BECN1- and PIK3C3-binding and for autophagy.By similarity

The final 80 residues in the C-terminus define a minimum required region for autophagosome binding called BATS.1 Publication

The N-terminal cysteine repeats are required for proper localization to the endoplasmic reticulum.By similarity

Sequence similaritiesⁱ

Belongs to the ATG14 family.Curated

Keywords - Domainⁱ

Coiled coil

Phylogenomic databases

eggNOGⁱ	KOG4398 Eukaryota ENOG410Y9W5 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000011854
HOGENOMⁱ	HOG000045344
HOVERGENⁱ	HBG081828
InParanoidⁱ	Q8CDJ3
KEGG Orthology (KO) More...KOⁱ	K17889
OMAⁱ	NTSYIRG
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G074J
PhylomeDBⁱ	Q8CDJ3
TreeFamⁱ	TF323392

Family and domain databases

InterProⁱ	View protein in InterPro IPR018791 UV_resistance/autophagy_Atg14
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF10186 Atg14, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q8CDJ3-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MASPSGKGSW TPEAPGFGPR ALARDLVDSV DDAEGLYVAV ERCPLCNTTR 
        60         70         80         90        100
RRLTCAKCVQ SGDFVYFDGR DRERFIDKKE RLSQLKNKQE EFQKEVLKAM 
       110        120        130        140        150
EGKRLTDQLR WKIMSCKMRI EQLKQTICKG NEEMKKNSEG LLKNKEKNQK 
       160        170        180        190        200
LYSRAQRHQE KKEKIQRHNR KLGDLVEKKT IDLKSHYERL ARLRRSHILE 
       210        220        230        240        250
LTSIIFPIDE VKTSGRDPAD VSSETDSAMT SSMVSKLAEA RRTTYLSGRW 
       260        270        280        290        300
VCDDHNGDTS ISITGPWISL PNNGDYSAYY NWVEEKKTTQ GPDMEHNNPA 
       310        320        330        340        350
YTISAALGYA TQLVNIVSHI LDINLPKKLC NSEFCGENLS KQKLTRAVRK 
       360        370        380        390        400
LNANILYLCS SQHVNLDQLQ PLHTLRNLMH LVSPRSEHLG RSGPFEVRAD 
       410        420        430        440        450
LEESMEFVDP GVAGESDASG DERVSDEETD LGTDWENLPS PRFCDIPSQP 
       460        470        480        490 
VEVSQSQSTQ VSPPIASSSA GGMISSAAAS VTSWFKAYTG HR

Length:492

Mass (Da):55,388

Last modified:March 1, 2003 - v1

Checksum:ⁱA221B3C03C8E2F9A

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A2I3BQC2	A0A2I3BQC2_MOUSE		Beclin 1-associated autophagy-relat... Beclin 1-associated autophagy-related key regulator	Atg14	125	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK029967 mRNA Translation: BAC26705.1 AK054196 mRNA Translation: BAC35689.1 BC025038 mRNA Translation: AAH25038.1 BC057360 mRNA Translation: AAH57360.1 BC090995 mRNA Translation: AAH90995.1 AK173037 mRNA Translation: BAD32315.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS26989.1
NCBI Reference Sequences More...RefSeqⁱ	NP_766187.1, NM_172599.4
UniGeneⁱ	Mm.287279 Mm.470462

Genome annotation databases

Ensemblⁱ	ENSMUST00000042988; ENSMUSP00000039047; ENSMUSG00000037526 ENSMUST00000226299; ENSMUSP00000153718; ENSMUSG00000037526
GeneIDⁱ	100504663
KEGGⁱ	mmu:100504663
UCSC genome browser More...UCSCⁱ	uc007tik.2 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q8CDJ3	Beclin 1-associated autophagy-related key regulator		HUMAN		492	UniRef90_Q6ZNE5
Beclin 1-associated autophagy-related key regulator		RAT		492
Autophagy related 14		SHEEP		512
Uncharacterized protein		CALJA		510
Uncharacterized protein		Cebus capucinus imitator		510
+112

Protein	Similar proteins		Species	Score	Length	Source
Q8CDJ3	Beclin 1-associated autophagy-related key regulator		HUMAN		492	UniRef50_Q6ZNE5
Beclin 1-associated autophagy-related key regulator		RAT		492
Autophagy related 14		SHEEP		512
Uncharacterized protein		PAPAN		510
Autophagy related 14		CHLSB		492
+247

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK029967 mRNA Translation: BAC26705.1 AK054196 mRNA Translation: BAC35689.1 BC025038 mRNA Translation: AAH25038.1 BC057360 mRNA Translation: AAH57360.1 BC090995 mRNA Translation: AAH90995.1 AK173037 mRNA Translation: BAD32315.1
CCDSⁱ	CCDS26989.1
RefSeqⁱ	NP_766187.1, NM_172599.4
UniGeneⁱ	Mm.287279 Mm.470462

3D structure databases

ProteinModelPortalⁱ	Q8CDJ3
SMRⁱ	Q8CDJ3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

ComplexPortalⁱ	CPX-75 Phosphatidylinositol 3-kinase complex, class III, ATG14 variant
DIPⁱ	DIP-60849N
IntActⁱ	Q8CDJ3, 11 interactors
STRINGⁱ	10090.ENSMUSP00000039047

PTM databases

iPTMnetⁱ	Q8CDJ3
PhosphoSitePlusⁱ	Q8CDJ3

Proteomic databases

MaxQBⁱ	Q8CDJ3
PaxDbⁱ	Q8CDJ3
PeptideAtlasⁱ	Q8CDJ3
PRIDEⁱ	Q8CDJ3

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000042988; ENSMUSP00000039047; ENSMUSG00000037526 ENSMUST00000226299; ENSMUSP00000153718; ENSMUSG00000037526
GeneIDⁱ	100504663
KEGGⁱ	mmu:100504663
UCSCⁱ	uc007tik.2 mouse

Organism-specific databases

CTDⁱ	22863
MGIⁱ	MGI:1261775 Atg14
Rougeⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG4398 Eukaryota ENOG410Y9W5 LUCA
GeneTreeⁱ	ENSGT00390000011854
HOGENOMⁱ	HOG000045344
HOVERGENⁱ	HBG081828
InParanoidⁱ	Q8CDJ3
KOⁱ	K17889
OMAⁱ	NTSYIRG
OrthoDBⁱ	EOG091G074J
PhylomeDBⁱ	Q8CDJ3
TreeFamⁱ	TF323392

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1632852 Macroautophagy

Reactomeⁱ

R-MMU-1632852 Macroautophagy

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q8CDJ3
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000037526 Expressed in 270 organ(s), highest expression level in morula
CleanExⁱ	MM_D14ERTD436E
Genevisibleⁱ	Q8CDJ3 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR018791 UV_resistance/autophagy_Atg14
Pfamⁱ	View protein in Pfam PF10186 Atg14, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	BAKOR_MOUSE
Accessionⁱ	Q8CDJ3Primary (citable) accession number: Q8CDJ3 Secondary accession number(s): Q69ZY1 , Q6PFY6, Q8C6N0, Q8R3M3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
	Last sequence update:	March 1, 2003
	Last modified:	November 7, 2018
	This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q8CDJ3 (BAKOR_MOUSE)

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Beclin 1-associated autophagy-related key regulator

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endoplasmic reticulum

Other locations

Cytoskeleton

Endoplasmic reticulum

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Coiled coil

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ