UniProtKB - Q8CDJ3 (BAKOR_MOUSE)
Beclin 1-associated autophagy-related key regulator
Atg14
Functioni
GO - Molecular functioni
- GTPase binding Source: MGI
GO - Biological processi
- autophagosome assembly Source: MGI
- autophagosome membrane docking Source: MGI
- autophagy Source: UniProtKB
- cellular response to glucose starvation Source: UniProtKB
- cellular response to starvation Source: MGI
- endosome to lysosome transport Source: MGI
- macroautophagy Source: MGI
- mitophagy Source: MGI
- negative regulation of protein phosphorylation Source: UniProtKB
- positive regulation of phosphatidylinositol 3-kinase activity Source: MGI
- positive regulation of protein phosphorylation Source: UniProtKB
- posttranscriptional regulation of gene expression Source: MGI
- regulation of protein phosphorylation Source: MGI
- regulation of triglyceride metabolic process Source: MGI
- response to mitochondrial depolarisation Source: MGI
Keywordsi
Biological process | Autophagy |
Enzyme and pathway databases
Reactomei | R-MMU-1632852, Macroautophagy |
Names & Taxonomyi
Protein namesi | Recommended name: Beclin 1-associated autophagy-related key regulatorBy similarityShort name: BarkorBy similarity Alternative name(s): Autophagy-related protein 14-like protein1 Publication Short name: Atg14L1 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1261775, Atg14 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Peripheral membrane protein Curated
Other locations
- Cytoplasm 1 Publication
- Preautophagosomal structure membrane 3 Publications; Peripheral membrane protein Curated
- autophagosome membrane By similarity; Peripheral membrane protein Curated
Note: Cytosolic under nutrient-rich conditions (PubMed:20639694). Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes (PubMed:20639694). Accumulates on highly curved PtdIns3P enriched autophagic membrane via its BATS domain to sense and maintain membrane curvature (PubMed:21518905). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (PubMed:24089209).3 Publications
Cytoskeleton
- axoneme Source: UniProtKB
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- autophagosome Source: MGI
- autophagosome membrane Source: UniProtKB-SubCell
- extrinsic component of omegasome membrane Source: MGI
- extrinsic component of phagophore assembly site membrane Source: MGI
- mitochondria-associated endoplasmic reticulum membrane Source: MGI
- phagocytic vesicle Source: MGI
- phagophore assembly site membrane Source: MGI
- phosphatidylinositol 3-kinase complex, class III Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050775 | 1 – 492 | Beclin 1-associated autophagy-related key regulatorAdd BLAST | 492 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 29 | PhosphoserineBy similarity | 1 | |
Modified residuei | 416 | PhosphoserineBy similarity | 1 | |
Modified residuei | 429 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8CDJ3 |
MaxQBi | Q8CDJ3 |
PaxDbi | Q8CDJ3 |
PeptideAtlasi | Q8CDJ3 |
PRIDEi | Q8CDJ3 |
PTM databases
iPTMneti | Q8CDJ3 |
PhosphoSitePlusi | Q8CDJ3 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000037526, Expressed in morula and 286 other tissues |
ExpressionAtlasi | Q8CDJ3, baseline and differential |
Genevisiblei | Q8CDJ3, MM |
Interactioni
Subunit structurei
Forms homooligomers; homo-oligomerization is essential for the roles in membrane tethering and enhancement of SNARE-mediated fusion (By similarity).
Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex I (PI3KC3-C1) in which the core composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 is associated with ATG14 (PubMed:19270693, PubMed:22745922, PubMed:23332761). PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex (By similarity). PI3KC3-C1 can associate with further regulatory subunits (PubMed:24849286).
Interacts with PIK3CB (PubMed:21059846).
Interacts (via coiled-coil domain) with BECN2 (via coiled-coil domain); this interaction is tighter than BECN2 self-association (By similarity).
Interacts with the STX17-SNAP29 binary t-SNARE complex (By similarity).
Interacts with NRBF2 (PubMed:24849286)
Interacts with PIK3C3 and BECN1; this interaction is increased in the absence of TMEM39A (By similarity).
By similarity5 PublicationsBinary interactionsi
Hide detailsGO - Molecular functioni
- GTPase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 425286, 3 interactors |
ComplexPortali | CPX-75, Phosphatidylinositol 3-kinase complex, class III, ATG14 variant |
DIPi | DIP-60849N |
IntActi | Q8CDJ3, 11 interactors |
STRINGi | 10090.ENSMUSP00000039047 |
Miscellaneous databases
RNActi | Q8CDJ3, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 413 – 492 | BATSAdd BLAST | 80 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 70 – 180 | Sequence analysisAdd BLAST | 111 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG4398, Eukaryota |
GeneTreei | ENSGT00390000011854 |
HOGENOMi | CLU_046719_1_0_1 |
InParanoidi | Q8CDJ3 |
OMAi | QHRSNED |
OrthoDBi | 1332122at2759 |
PhylomeDBi | Q8CDJ3 |
TreeFami | TF323392 |
Family and domain databases
InterProi | View protein in InterPro IPR018791, UV_resistance/autophagy_Atg14 |
Pfami | View protein in Pfam PF10186, Atg14, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MASPSGKGSW TPEAPGFGPR ALARDLVDSV DDAEGLYVAV ERCPLCNTTR
60 70 80 90 100
RRLTCAKCVQ SGDFVYFDGR DRERFIDKKE RLSQLKNKQE EFQKEVLKAM
110 120 130 140 150
EGKRLTDQLR WKIMSCKMRI EQLKQTICKG NEEMKKNSEG LLKNKEKNQK
160 170 180 190 200
LYSRAQRHQE KKEKIQRHNR KLGDLVEKKT IDLKSHYERL ARLRRSHILE
210 220 230 240 250
LTSIIFPIDE VKTSGRDPAD VSSETDSAMT SSMVSKLAEA RRTTYLSGRW
260 270 280 290 300
VCDDHNGDTS ISITGPWISL PNNGDYSAYY NWVEEKKTTQ GPDMEHNNPA
310 320 330 340 350
YTISAALGYA TQLVNIVSHI LDINLPKKLC NSEFCGENLS KQKLTRAVRK
360 370 380 390 400
LNANILYLCS SQHVNLDQLQ PLHTLRNLMH LVSPRSEHLG RSGPFEVRAD
410 420 430 440 450
LEESMEFVDP GVAGESDASG DERVSDEETD LGTDWENLPS PRFCDIPSQP
460 470 480 490
VEVSQSQSTQ VSPPIASSSA GGMISSAAAS VTSWFKAYTG HR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A2I3BQC2 | A0A2I3BQC2_MOUSE | Beclin 1-associated autophagy-relat... | Atg14 | 125 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK029967 mRNA Translation: BAC26705.1 AK054196 mRNA Translation: BAC35689.1 BC025038 mRNA Translation: AAH25038.1 BC057360 mRNA Translation: AAH57360.1 BC090995 mRNA Translation: AAH90995.1 AK173037 mRNA Translation: BAD32315.1 |
CCDSi | CCDS26989.1 |
RefSeqi | NP_766187.1, NM_172599.4 |
Genome annotation databases
Ensembli | ENSMUST00000042988; ENSMUSP00000039047; ENSMUSG00000037526 ENSMUST00000226299; ENSMUSP00000153718; ENSMUSG00000037526 |
GeneIDi | 100504663 |
KEGGi | mmu:100504663 |
UCSCi | uc007tik.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK029967 mRNA Translation: BAC26705.1 AK054196 mRNA Translation: BAC35689.1 BC025038 mRNA Translation: AAH25038.1 BC057360 mRNA Translation: AAH57360.1 BC090995 mRNA Translation: AAH90995.1 AK173037 mRNA Translation: BAD32315.1 |
CCDSi | CCDS26989.1 |
RefSeqi | NP_766187.1, NM_172599.4 |
3D structure databases
SMRi | Q8CDJ3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 425286, 3 interactors |
ComplexPortali | CPX-75, Phosphatidylinositol 3-kinase complex, class III, ATG14 variant |
DIPi | DIP-60849N |
IntActi | Q8CDJ3, 11 interactors |
STRINGi | 10090.ENSMUSP00000039047 |
PTM databases
iPTMneti | Q8CDJ3 |
PhosphoSitePlusi | Q8CDJ3 |
Proteomic databases
EPDi | Q8CDJ3 |
MaxQBi | Q8CDJ3 |
PaxDbi | Q8CDJ3 |
PeptideAtlasi | Q8CDJ3 |
PRIDEi | Q8CDJ3 |
Protocols and materials databases
Antibodypediai | 24031, 200 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000042988; ENSMUSP00000039047; ENSMUSG00000037526 ENSMUST00000226299; ENSMUSP00000153718; ENSMUSG00000037526 |
GeneIDi | 100504663 |
KEGGi | mmu:100504663 |
UCSCi | uc007tik.2, mouse |
Organism-specific databases
CTDi | 22863 |
MGIi | MGI:1261775, Atg14 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG4398, Eukaryota |
GeneTreei | ENSGT00390000011854 |
HOGENOMi | CLU_046719_1_0_1 |
InParanoidi | Q8CDJ3 |
OMAi | QHRSNED |
OrthoDBi | 1332122at2759 |
PhylomeDBi | Q8CDJ3 |
TreeFami | TF323392 |
Enzyme and pathway databases
Reactomei | R-MMU-1632852, Macroautophagy |
Miscellaneous databases
BioGRID-ORCSi | 100504663, 5 hits in 17 CRISPR screens |
PROi | PR:Q8CDJ3 |
RNActi | Q8CDJ3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037526, Expressed in morula and 286 other tissues |
ExpressionAtlasi | Q8CDJ3, baseline and differential |
Genevisiblei | Q8CDJ3, MM |
Family and domain databases
InterProi | View protein in InterPro IPR018791, UV_resistance/autophagy_Atg14 |
Pfami | View protein in Pfam PF10186, Atg14, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BAKOR_MOUSE | |
Accessioni | Q8CDJ3Primary (citable) accession number: Q8CDJ3 Secondary accession number(s): Q69ZY1 Q8R3M3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 124 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families