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Protein

Deubiquitinating protein VCIP135

Gene

Vcpip1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a deubiquitinating enzyme. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP. Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei215By similarity1
Active sitei218NucleophileBy similarity1
Active sitei353By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deubiquitinating protein VCIP135 (EC:3.4.19.12)
Alternative name(s):
Valosin-containing protein p97/p47 complex-interacting protein 1
Valosin-containing protein p97/p47 complex-interacting protein p135
Gene namesi
Name:Vcpip1
Synonyms:Vcip135
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1917925 Vcpip1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000657701 – 1220Deubiquitinating protein VCIP135Add BLAST1220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei407N6-acetyllysineBy similarity1
Modified residuei746PhosphoserineBy similarity1
Modified residuei756PhosphoserineBy similarity1
Modified residuei762PhosphothreonineBy similarity1
Modified residuei767PhosphoserineBy similarity1
Modified residuei993PhosphoserineBy similarity1
Modified residuei997PhosphoserineBy similarity1
Modified residuei1076PhosphoserineBy similarity1
Modified residuei1196PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8CDG3
PaxDbiQ8CDG3
PeptideAtlasiQ8CDG3
PRIDEiQ8CDG3

PTM databases

iPTMnetiQ8CDG3
PhosphoSitePlusiQ8CDG3

Interactioni

Subunit structurei

Binds VCP and the ternary complex containing STX5A, NSFL1C and VCP.By similarity

Protein-protein interaction databases

BioGridi214195, 13 interactors
IntActiQ8CDG3, 13 interactors
MINTiQ8CDG3
STRINGi10090.ENSMUSP00000051248

Structurei

3D structure databases

ProteinModelPortaliQ8CDG3
SMRiQ8CDG3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini207 – 360OTUPROSITE-ProRule annotationAdd BLAST154

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 20Pro-richAdd BLAST17

Phylogenomic databases

eggNOGiENOG410IQZJ Eukaryota
ENOG410ZMR9 LUCA
HOVERGENiHBG059748
InParanoidiQ8CDG3
KOiK11861
PhylomeDBiQ8CDG3
TreeFamiTF329469

Family and domain databases

InterProiView protein in InterPro
IPR003323 OTU_dom
IPR029071 Ubiquitin-like_domsf
IPR039087 VCIP135
PANTHERiPTHR14843 PTHR14843, 1 hit
PfamiView protein in Pfam
PF02338 OTU, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50802 OTU, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8CDG3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSQPPPPPPL PPPPPPPEAP QTSSSLAAAA SPGGLSKRRD RRILSGSCPD
60 70 80 90 100
PKCQARLFFP ASGSVSIECT ECGQRHEQQQ LLGVEEVTDP DVVLHNLLRN
110 120 130 140 150
ALLGVTGAPK KNTELVKVMG LSNYHCKLLS PILARYGMDK QTGRAKLLRD
160 170 180 190 200
MNQGELFDCA LLGDRAFLIE PEHVNTVGYG KDRSGSLLYL HDTLEDIKRA
210 220 230 240 250
NKSQECLIPV HVDGDGHCLV HAVSRALVGR ELFWHALREN LKQHFQQHLA
260 270 280 290 300
RYQALFHDFI DAAEWEDIIN ECDPLFVPPE GVPLGLRNIH IFGLANVLHR
310 320 330 340 350
PIILLDSLSG MRSSGDYSAT FLPGLIPAEK CTGRDGHLNK PICIAWSSSG
360 370 380 390 400
RNHYIPLVGI KGAALPKLPM NLLPKAWGVP QDLIKKYIKL EEDGGCVIGG
410 420 430 440 450
DRSLQDKYLL RLVAAMEEVF MDKHGIHPSL VADVHQYFYR RTGVIGVQPE
460 470 480 490 500
EVTAAAKKAV MDNRLHKCLL YGALSELHVP SEWLAPGGKL YNLAKSTHGQ
510 520 530 540 550
LRPDKNYSFP LNNLVCSYDP VKDVLLPDYG LSNLTACNWC HGSSVRRVRG
560 570 580 590 600
DGSIVYLDGD RTNSRSTGGK CGCGFKHFWE GKEYDNLPEA FPITLEWGGR
610 620 630 640 650
VVRETVYWFQ YESDPSLNSN VYDVAMKLVT KHFPGEFGSE ILVQKVVHTI
660 670 680 690 700
LHQTAKKNPD DYTPVNIDGA HAQRVGDVQG QELESQLPTK IILTGQKTKT
710 720 730 740 750
LHKEELNMSK TERTIQQNIT EQASVMQKRK TEKLKQEQKG QPRTVSPSTI
760 770 780 790 800
RDGPSSAPAT PTKAPYSPTT SKEKKIRITT NDGRQSMVTL KPSTTFFELQ
810 820 830 840 850
ESIAREFNIP PYLQCIRYGF PPKELMPPQA GMEKEPVPLQ HGDRITIEIL
860 870 880 890 900
KGRAEGGPST AAHSAHTVKQ EEIAVTGKLS SKELQEQADK EMYSLCLLAT
910 920 930 940 950
LMGEDVWSYA KGLPHMFQQG GVFYNIMKKT MGMADGKHCT FPHLPGKTFV
960 970 980 990 1000
YNASEDRLEL CVDAAGHFPI GPDVEDLVKE AVSQVRAEAT TRSRESSPSH
1010 1020 1030 1040 1050
GLLKLGSGGV VKKKSEQLHN VTAFQGKGHS LGTASSHPHI DPRARETLAV
1060 1070 1080 1090 1100
RKHNTGTDFS NSSIKTEPPV FTAASSNSEL IRIAPGVVTM RDGRQIDPDV
1110 1120 1130 1140 1150
VEAQRKKLQE MVSSIQASMD KHLRDQSAEQ APSDLSQRKV EVVSSVRPVN
1160 1170 1180 1190 1200
LQTGLPEPFS LTGGTENLNT ETTDSHVADV LGAAFATRSK AQKENSMEEP
1210 1220
EEMDSQDAET TNTTEPMDHS
Length:1,220
Mass (Da):134,503
Last modified:March 1, 2003 - v1
Checksum:i8062E3C17DCD2E3B
GO
Isoform 2 (identifier: Q8CDG3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     904-906: EDV → KSN
     907-1220: Missing.

Note: May be due to an intron retention. No experimental confirmation available.
Show »
Length:906
Mass (Da):100,374
Checksum:i700778A8E7031FFE
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J0M9A0A0R4J0M9_MOUSE
Deubiquitinating protein VCIP135
Vcpip1
1,220Annotation score:

Sequence cautioni

The sequence AAH52908 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti471Y → C in AAH59209 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009376904 – 906EDV → KSN in isoform 2. 1 Publication3
Alternative sequenceiVSP_009377907 – 1220Missing in isoform 2. 1 PublicationAdd BLAST314

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030104 mRNA Translation: BAC26787.1
AK077494 mRNA Translation: BAC36830.1
BC052908 mRNA Translation: AAH52908.1 Different initiation.
BC059209 mRNA Translation: AAH59209.1
CCDSiCCDS14814.1 [Q8CDG3-1]
RefSeqiNP_775619.2, NM_173443.3
UniGeneiMm.274493
Mm.440951

Genome annotation databases

GeneIDi70675
KEGGimmu:70675
UCSCiuc007ags.3 mouse [Q8CDG3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030104 mRNA Translation: BAC26787.1
AK077494 mRNA Translation: BAC36830.1
BC052908 mRNA Translation: AAH52908.1 Different initiation.
BC059209 mRNA Translation: AAH59209.1
CCDSiCCDS14814.1 [Q8CDG3-1]
RefSeqiNP_775619.2, NM_173443.3
UniGeneiMm.274493
Mm.440951

3D structure databases

ProteinModelPortaliQ8CDG3
SMRiQ8CDG3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214195, 13 interactors
IntActiQ8CDG3, 13 interactors
MINTiQ8CDG3
STRINGi10090.ENSMUSP00000051248

PTM databases

iPTMnetiQ8CDG3
PhosphoSitePlusiQ8CDG3

Proteomic databases

EPDiQ8CDG3
PaxDbiQ8CDG3
PeptideAtlasiQ8CDG3
PRIDEiQ8CDG3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi70675
KEGGimmu:70675
UCSCiuc007ags.3 mouse [Q8CDG3-1]

Organism-specific databases

CTDi80124
MGIiMGI:1917925 Vcpip1

Phylogenomic databases

eggNOGiENOG410IQZJ Eukaryota
ENOG410ZMR9 LUCA
HOVERGENiHBG059748
InParanoidiQ8CDG3
KOiK11861
PhylomeDBiQ8CDG3
TreeFamiTF329469

Miscellaneous databases

ChiTaRSiVcpip1 mouse
PROiPR:Q8CDG3
SOURCEiSearch...

Family and domain databases

InterProiView protein in InterPro
IPR003323 OTU_dom
IPR029071 Ubiquitin-like_domsf
IPR039087 VCIP135
PANTHERiPTHR14843 PTHR14843, 1 hit
PfamiView protein in Pfam
PF02338 OTU, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50802 OTU, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVCIP1_MOUSE
AccessioniPrimary (citable) accession number: Q8CDG3
Secondary accession number(s): Q7TMU9, Q8BP90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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