UniProtKB - Q8CDA1 (SAC2_MOUSE)
Protein
Phosphatidylinositide phosphatase SAC2
Gene
Inpp5f
Organism
Mus musculus (Mouse)
Status
Functioni
Inositol 4-phosphatase which mainly acts on phosphatidylinositol 4-phosphate. May be functionally linked to OCRL, which converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol, for a sequential dephosphorylation of phosphatidylinositol 4,5-bisphosphate at the 5 and 4 position of inositol, thus playing an important role in the endocytic recycling (PubMed:25869668, PubMed:25869669). Regulator of TF:TFRC and integrins recycling pathway, is also involved in cell migration mechanisms (By similarity). Modulates AKT/GSK3B pathway by decreasing AKT and GSK3B phosphorylation (PubMed:17322895). Negatively regulates STAT3 signaling pathway through inhibition of STAT3 phosphorylation and translocation to the nucleus (By similarity). Functionally important modulator of cardiac myocyte size and of the cardiac response to stress (PubMed:19875726). May play a role as negative regulator of axon regeneration after central nervous system injuries (PubMed:26203138).By similarity5 Publications
Caution
INPP5F has been initially described as an inositol polyphosphate 5-phosphatase.By similarity
Catalytic activityi
- EC:3.1.3.25By similarity
GO - Molecular functioni
- inositol monophosphate 1-phosphatase activity Source: UniProtKB-EC
- inositol monophosphate 3-phosphatase activity Source: UniProtKB-EC
- inositol monophosphate 4-phosphatase activity Source: UniProtKB
- phosphatidylinositol-4-phosphate phosphatase activity Source: GO_Central
- phosphatidylinositol phosphate 4-phosphatase activity Source: ParkinsonsUK-UCL
- phosphatidylinositol phosphate 5-phosphatase activity Source: Ensembl
- protein homodimerization activity Source: MGI
GO - Biological processi
- adult locomotory behavior Source: ParkinsonsUK-UCL
- cardiac muscle hypertrophy in response to stress Source: MGI
- clathrin-dependent endocytosis Source: UniProtKB
- negative regulation of axon regeneration Source: ParkinsonsUK-UCL
- negative regulation of peptidyl-serine phosphorylation Source: MGI
- negative regulation of tyrosine phosphorylation of STAT protein Source: UniProtKB
- phosphatidylinositol catabolic process Source: MGI
- phosphatidylinositol dephosphorylation Source: ParkinsonsUK-UCL
- phosphatidylinositol-mediated signaling Source: MGI
- positive regulation of receptor recycling Source: ParkinsonsUK-UCL
- regulation of cell motility Source: UniProtKB
- regulation of endocytic recycling Source: UniProtKB
- regulation of protein kinase B signaling Source: MGI
Keywordsi
Molecular function | Hydrolase |
Enzyme and pathway databases
Reactomei | R-MMU-1660516, Synthesis of PIPs at the early endosome membrane |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphatidylinositide phosphatase SAC2 (EC:3.1.3.25By similarity1 Publication)Alternative name(s): Inositol polyphosphate 5-phosphatase FImported Sac domain-containing inositol phosphatase 2 Sac domain-containing phosphoinositide 4-phosphatase 21 Publication Short name: hSAC2 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2141867, Inpp5f |
Subcellular locationi
Endosome
- Early endosome 1 Publication
- Recycling endosome By similarity
Other locations
- clathrin-coated pit 1 Publication
Note: Also found on macropinosomes.1 Publication
Endosome
- early endosome Source: UniProtKB
- recycling endosome Source: UniProtKB
Plasma Membrane
- clathrin-coated pit Source: UniProtKB-SubCell
Other locations
- axon Source: ParkinsonsUK-UCL
- clathrin-coated endocytic vesicle Source: UniProtKB
- cytoplasm Source: ParkinsonsUK-UCL
- dendrite Source: ParkinsonsUK-UCL
- intracellular membrane-bounded organelle Source: MGI
- neuronal cell body Source: ParkinsonsUK-UCL
Keywords - Cellular componenti
Coated pit, Endosome, MembranePathology & Biotechi
Disruption phenotypei
Animals develop normal corticospinal tract and raphespinal tract. Mutants show greater axonal growth and functional recovery after central nervous system trauma (PubMed:26203138). Knockout mice have normal cardiac form and function but show augmented hypertrophy and reactivation of the fetal gene program in response to stress compared to wild-type littermates (PubMed:19875726).2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 460 | D → A: Has a diffuse cytosolic localization. 1 Publication | 1 | |
Mutagenesisi | 460 | D → N: Loss of inositol 4-phosphatase activity. No effect on subcellular localization. No effect on interaction with OCRL, INPP5B and IPP4A. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000331622 | 1 – 1132 | Phosphatidylinositide phosphatase SAC2Add BLAST | 1132 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 714 | PhosphoserineCombined sources | 1 | |
Modified residuei | 827 | PhosphoserineCombined sources | 1 | |
Modified residuei | 830 | PhosphoserineCombined sources | 1 | |
Modified residuei | 879 | PhosphoserineCombined sources | 1 | |
Modified residuei | 882 | PhosphoserineCombined sources | 1 | |
Modified residuei | 908 | PhosphoserineCombined sources | 1 | |
Modified residuei | 911 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1103 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q8CDA1 |
PaxDbi | Q8CDA1 |
PeptideAtlasi | Q8CDA1 |
PRIDEi | Q8CDA1 |
PTM databases
iPTMneti | Q8CDA1 |
PhosphoSitePlusi | Q8CDA1 |
Expressioni
Tissue specificityi
Highly expressed in brain and hypothalamus, expressed in lung and pancreas, and detected at low levels in liver and heart (at protein level).2 Publications
Inductioni
Up-regulated in the absence of histone deacetylase 2/HDAC2 in the heart from HDAC2-null mice.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000042105, Expressed in central nervous system and 267 other tissues |
Genevisiblei | Q8CDA1, MM |
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with OCRL and RAB5.
Interacts with INPP5B and INPP4A (PubMed:25869668).
Interacts with STAT3; the interaction is independent of STAT3 'TYR-705' phosphorylation status (By similarity).
By similarity1 PublicationGO - Molecular functioni
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000045910 |
Miscellaneous databases
RNActi | Q8CDA1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 167 – 518 | SACPROSITE-ProRule annotationAdd BLAST | 352 | |
Domaini | 593 – 760 | hSac2PROSITE-ProRule annotationAdd BLAST | 168 |
Phylogenomic databases
eggNOGi | KOG1890, Eukaryota |
GeneTreei | ENSGT00940000155996 |
HOGENOMi | CLU_044255_0_0_1 |
InParanoidi | Q8CDA1 |
OMAi | DNCCLEL |
PhylomeDBi | Q8CDA1 |
TreeFami | TF313543 |
Family and domain databases
InterProi | View protein in InterPro IPR034753, hSac2 IPR022158, Inositol_phosphatase IPR002013, SAC_dom |
Pfami | View protein in Pfam PF12456, hSac2, 1 hit PF02383, Syja_N, 1 hit |
PROSITEi | View protein in PROSITE PS51791, HSAC2, 1 hit PS50275, SAC, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8CDA1-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MELFQAKDHY ILQQGERALW CSRRDGGLQL RPATDLLLAW NPICLGLVEG
60 70 80 90 100
VIGKIQLHSD LPWWLILIRQ KALVGKLPGD HEVCKVTKIA VLSLSEMEPQ
110 120 130 140 150
ELELELCKKH HFGINKPEKI IPSPDDSKFL LKTFTNIKSN VSAPNKKKVK
160 170 180 190 200
ESKEKEKLER RLLEELLKMF MDSESFYYSL TYDLTNSVQR QSTGERDGRP
210 220 230 240 250
LWQKVDDRFF WNKYMIQALT EIGTPDVDFW IIPIIQGFVQ IEELVVNYNE
260 270 280 290 300
SSDDDKSSPE TPPQDSTCVD DIHPRFLVAL ISRRSRHRAG MRYKRRGVDK
310 320 330 340 350
NGNVANYVET EQLIHVHHHT LSFIQTRGSV PVFWSQVGYR YNPRPRLDKS
360 370 380 390 400
EKETVDCFCA HFEEQLKIYK KQVIVNLVDQ AGREKIIGDA YLKQVLLFNN
410 420 430 440 450
PKLTYVSFDF HEHCRGMKFE NVQTLTDAIH DIIIDMKWCW VDQAGVICKQ
460 470 480 490 500
EGIFRVNCMD CLDRTNVVQA AIARVVMEQQ LKKLGVMPPE QPLPVKCNRT
510 520 530 540 550
YQIMWANNGD SISRQYAGTA ALKGDFTRTG ERKLAGVMKD GVNSANRYYL
560 570 580 590 600
SRFKDAYRQA VIDLMQGVPV TEDLYSIFTK EKEHEALHKE SQRSHQELIS
610 620 630 640 650
QLLQSYMQLL LPGDEKFHGG WALVDCDPSL TDAAHRDVEV LLLLSNAAYY
660 670 680 690 700
VAYYDDEVDK VNQYQRLGLE DLERIEIGPE PTLFGKPKFS CMRLHYRCKE
710 720 730 740 750
AGGYFHTLRA VPRSPEEDGK DTLQCIAEML QITKQAMGLD VPIIEKKLER
760 770 780 790 800
KSSKPHEDII GIRSQNQGSL AQGKSFLMSK FSSLNQKVKQ TKSNVNIGNL
810 820 830 840 850
RKLGNFTKPE MKVNFLKPNL KVNLWKSDSS LETMENPGVM GNKVQGESDG
860 870 880 890 900
DISSDNDSYH SDEFLTNSKS EEDKQLANSL ESVGPIDYIL PSCGIIVSAP
910 920 930 940 950
RLGSRSQSAS SIDVSTHAPS EAAAGPGSEL GKGLESPLKK SPSADSIHTR
960 970 980 990 1000
TGFTKPMDVY CQRFVQDAQN KMNDLSEIRS VAQKSEEGSH KTNRVSNEET
1010 1020 1030 1040 1050
QSEPMGQTPP RPSQLNVSCS VAGPPFLSVE PVHSVLSQKT PSSGSSLLEL
1060 1070 1080 1090 1100
EAGLCVTPSS ESSSSRAVSP FAKIRSSMVQ VANITQAGLT HGINLAVAKV
1110 1120 1130
QKSPAEPEAV NEIQQNELKN MFTQCQTRII QI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0J9YU13 | A0A0J9YU13_MOUSE | Phosphatidylinositide phosphatase S... | Inpp5f | 121 | Annotation score: |
Sequence cautioni
The sequence BAC28723 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 728 | E → D in AAH67200 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_033270 | 1 – 691 | Missing in isoform 3. 1 PublicationAdd BLAST | 691 | |
Alternative sequenceiVSP_033271 | 1 – 627 | Missing in isoform 2. 1 PublicationAdd BLAST | 627 | |
Alternative sequenceiVSP_033272 | 373 – 414 | VIVNL…FHEHC → RIWVWSQHPLTQREEKRREE KRREEKRREEKRREEKRREE VT in isoform 4. 1 PublicationAdd BLAST | 42 | |
Alternative sequenceiVSP_033273 | 415 – 1132 | Missing in isoform 4. 1 PublicationAdd BLAST | 718 | |
Alternative sequenceiVSP_033274 | 628 – 629 | PS → MH in isoform 2. 1 Publication | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK129249 mRNA Translation: BAC98059.1 AK030870 mRNA Translation: BAC27166.1 AK034482 mRNA Translation: BAC28723.1 Frameshift. AK047166 mRNA Translation: BAC32978.1 AK150418 mRNA Translation: BAE29542.1 BC067200 mRNA Translation: AAH67200.1 BC125437 mRNA Translation: AAI25438.1 |
CCDSi | CCDS21899.1 [Q8CDA1-1] CCDS85430.1 [Q8CDA1-2] |
RefSeqi | NP_001333446.1, NM_001346517.1 [Q8CDA1-2] NP_848756.2, NM_178641.5 [Q8CDA1-1] |
Genome annotation databases
Ensembli | ENSMUST00000043138; ENSMUSP00000045910; ENSMUSG00000042105 [Q8CDA1-1] ENSMUST00000118605; ENSMUSP00000113700; ENSMUSG00000042105 [Q8CDA1-2] ENSMUST00000151237; ENSMUSP00000146197; ENSMUSG00000042105 [Q8CDA1-3] |
GeneIDi | 101490 |
KEGGi | mmu:101490 |
UCSCi | uc009jzc.1, mouse [Q8CDA1-1] uc009jzf.1, mouse [Q8CDA1-3] uc009jzg.1, mouse [Q8CDA1-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK129249 mRNA Translation: BAC98059.1 AK030870 mRNA Translation: BAC27166.1 AK034482 mRNA Translation: BAC28723.1 Frameshift. AK047166 mRNA Translation: BAC32978.1 AK150418 mRNA Translation: BAE29542.1 BC067200 mRNA Translation: AAH67200.1 BC125437 mRNA Translation: AAI25438.1 |
CCDSi | CCDS21899.1 [Q8CDA1-1] CCDS85430.1 [Q8CDA1-2] |
RefSeqi | NP_001333446.1, NM_001346517.1 [Q8CDA1-2] NP_848756.2, NM_178641.5 [Q8CDA1-1] |
3D structure databases
SMRi | Q8CDA1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000045910 |
PTM databases
iPTMneti | Q8CDA1 |
PhosphoSitePlusi | Q8CDA1 |
Proteomic databases
jPOSTi | Q8CDA1 |
PaxDbi | Q8CDA1 |
PeptideAtlasi | Q8CDA1 |
PRIDEi | Q8CDA1 |
Protocols and materials databases
Antibodypediai | 32161, 88 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000043138; ENSMUSP00000045910; ENSMUSG00000042105 [Q8CDA1-1] ENSMUST00000118605; ENSMUSP00000113700; ENSMUSG00000042105 [Q8CDA1-2] ENSMUST00000151237; ENSMUSP00000146197; ENSMUSG00000042105 [Q8CDA1-3] |
GeneIDi | 101490 |
KEGGi | mmu:101490 |
UCSCi | uc009jzc.1, mouse [Q8CDA1-1] uc009jzf.1, mouse [Q8CDA1-3] uc009jzg.1, mouse [Q8CDA1-2] |
Organism-specific databases
CTDi | 22876 |
MGIi | MGI:2141867, Inpp5f |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG1890, Eukaryota |
GeneTreei | ENSGT00940000155996 |
HOGENOMi | CLU_044255_0_0_1 |
InParanoidi | Q8CDA1 |
OMAi | DNCCLEL |
PhylomeDBi | Q8CDA1 |
TreeFami | TF313543 |
Enzyme and pathway databases
Reactomei | R-MMU-1660516, Synthesis of PIPs at the early endosome membrane |
Miscellaneous databases
BioGRID-ORCSi | 101490, 0 hits in 17 CRISPR screens |
ChiTaRSi | Inpp5f, mouse |
PROi | PR:Q8CDA1 |
RNActi | Q8CDA1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000042105, Expressed in central nervous system and 267 other tissues |
Genevisiblei | Q8CDA1, MM |
Family and domain databases
InterProi | View protein in InterPro IPR034753, hSac2 IPR022158, Inositol_phosphatase IPR002013, SAC_dom |
Pfami | View protein in Pfam PF12456, hSac2, 1 hit PF02383, Syja_N, 1 hit |
PROSITEi | View protein in PROSITE PS51791, HSAC2, 1 hit PS50275, SAC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SAC2_MOUSE | |
Accessioni | Q8CDA1Primary (citable) accession number: Q8CDA1 Secondary accession number(s): Q3UCS0 Q8CBW2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 29, 2008 |
Last sequence update: | March 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot