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Protein

Phosphatidylinositide phosphatase SAC2

Gene

Inpp5f

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inositol 4-phosphatase which mainly acts on phosphatidylinositol 4-phosphate. May be functionally linked to OCRL, which converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol, for a sequential dephosphorylation of phosphatidylinositol 4,5-bisphosphate at the 5 and 4 position of inositol, thus playing an important role in the endocytic recycling (PubMed:25869668, PubMed:25869669). Regulator of TF:TFRC and integrins recycling pathway, is also involved in cell migration mechanisms (By similarity). Modulates AKT/GSK3B pathway by decreasing AKT and GSK3B phosphorylation (PubMed:17322895). Negatively regulates STAT3 signaling pathway through inhibition of STAT3 phosphorylation and translocation to the nucleus (By similarity). Functionally important modulator of cardiac myocyte size and of the cardiac response to stress (PubMed:19875726). May play a role as negative regulator of axon regeneration after central nervous system injuries (PubMed:26203138).By similarity5 Publications

Caution

INPP5F has been initially described as an inositol polyphosphate 5-phosphatase.By similarity

Catalytic activityi

Myo-inositol phosphate + H2O = myo-inositol + phosphate.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

ReactomeiR-MMU-1660516 Synthesis of PIPs at the early endosome membrane

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositide phosphatase SAC2 (EC:3.1.3.25By similarity1 Publication)
Alternative name(s):
Inositol polyphosphate 5-phosphatase FImported
Sac domain-containing inositol phosphatase 2
Sac domain-containing phosphoinositide 4-phosphatase 21 Publication
Short name:
hSAC2
Gene namesi
Name:Inpp5fImported
Synonyms:Kiaa0966, Sac2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2141867 Inpp5f

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Coated pit, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Animals develop normal corticospinal tract and raphespinal tract. Mutants show greater axonal growth and functional recovery after central nervous system trauma (PubMed:26203138). Knockout mice have normal cardiac form and function but show augmented hypertrophy and reactivation of the fetal gene program in response to stress compared to wild-type littermates (PubMed:19875726).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi460D → A: Has a diffuse cytosolic localization. 1 Publication1
Mutagenesisi460D → N: Loss of inositol 4-phosphatase activity. No effect on subcellular localization. No effect on interaction with OCRL, INPP5B and IPP4A. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003316221 – 1132Phosphatidylinositide phosphatase SAC2Add BLAST1132

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei714PhosphoserineCombined sources1
Modified residuei827PhosphoserineCombined sources1
Modified residuei830PhosphoserineCombined sources1
Modified residuei879PhosphoserineCombined sources1
Modified residuei882PhosphoserineCombined sources1
Modified residuei908PhosphoserineCombined sources1
Modified residuei911PhosphoserineCombined sources1
Modified residuei1103PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8CDA1
PeptideAtlasiQ8CDA1
PRIDEiQ8CDA1

PTM databases

iPTMnetiQ8CDA1
PhosphoSitePlusiQ8CDA1

Expressioni

Tissue specificityi

Highly expressed in brain and hypothalamus, expressed in lung and pancreas, and detected at low levels in liver and heart (at protein level).2 Publications

Inductioni

Up-regulated in the absence of histone deacetylase 2/HDAC2 in the heart from HDAC2-null mice.1 Publication

Gene expression databases

BgeeiENSMUSG00000042105 Expressed in 251 organ(s), highest expression level in central nervous system
ExpressionAtlasiQ8CDA1 baseline and differential
GenevisibleiQ8CDA1 MM

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with OCRL and RAB5. Interacts with INPP5B and INPP4A (PubMed:25869668). Interacts with STAT3; the interaction is independent of STAT3 'TYR-705' phosphorylation status (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045910

Structurei

3D structure databases

ProteinModelPortaliQ8CDA1
SMRiQ8CDA1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini167 – 518SACPROSITE-ProRule annotationAdd BLAST352
Domaini593 – 760hSac2PROSITE-ProRule annotationAdd BLAST168

Phylogenomic databases

eggNOGiKOG1890 Eukaryota
COG5329 LUCA
GeneTreeiENSGT00530000063393
HOGENOMiHOG000046510
HOVERGENiHBG095361
InParanoidiQ8CDA1
KOiK21798
OMAiAYCHQFV
OrthoDBiEOG091G059J
PhylomeDBiQ8CDA1
TreeFamiTF313543

Family and domain databases

InterProiView protein in InterPro
IPR034753 hSac2
IPR022158 Inositol_phosphatase
IPR002013 SAC_dom
PfamiView protein in Pfam
PF12456 hSac2, 1 hit
PF02383 Syja_N, 1 hit
PROSITEiView protein in PROSITE
PS51791 HSAC2, 1 hit
PS50275 SAC, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8CDA1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MELFQAKDHY ILQQGERALW CSRRDGGLQL RPATDLLLAW NPICLGLVEG
60 70 80 90 100
VIGKIQLHSD LPWWLILIRQ KALVGKLPGD HEVCKVTKIA VLSLSEMEPQ
110 120 130 140 150
ELELELCKKH HFGINKPEKI IPSPDDSKFL LKTFTNIKSN VSAPNKKKVK
160 170 180 190 200
ESKEKEKLER RLLEELLKMF MDSESFYYSL TYDLTNSVQR QSTGERDGRP
210 220 230 240 250
LWQKVDDRFF WNKYMIQALT EIGTPDVDFW IIPIIQGFVQ IEELVVNYNE
260 270 280 290 300
SSDDDKSSPE TPPQDSTCVD DIHPRFLVAL ISRRSRHRAG MRYKRRGVDK
310 320 330 340 350
NGNVANYVET EQLIHVHHHT LSFIQTRGSV PVFWSQVGYR YNPRPRLDKS
360 370 380 390 400
EKETVDCFCA HFEEQLKIYK KQVIVNLVDQ AGREKIIGDA YLKQVLLFNN
410 420 430 440 450
PKLTYVSFDF HEHCRGMKFE NVQTLTDAIH DIIIDMKWCW VDQAGVICKQ
460 470 480 490 500
EGIFRVNCMD CLDRTNVVQA AIARVVMEQQ LKKLGVMPPE QPLPVKCNRT
510 520 530 540 550
YQIMWANNGD SISRQYAGTA ALKGDFTRTG ERKLAGVMKD GVNSANRYYL
560 570 580 590 600
SRFKDAYRQA VIDLMQGVPV TEDLYSIFTK EKEHEALHKE SQRSHQELIS
610 620 630 640 650
QLLQSYMQLL LPGDEKFHGG WALVDCDPSL TDAAHRDVEV LLLLSNAAYY
660 670 680 690 700
VAYYDDEVDK VNQYQRLGLE DLERIEIGPE PTLFGKPKFS CMRLHYRCKE
710 720 730 740 750
AGGYFHTLRA VPRSPEEDGK DTLQCIAEML QITKQAMGLD VPIIEKKLER
760 770 780 790 800
KSSKPHEDII GIRSQNQGSL AQGKSFLMSK FSSLNQKVKQ TKSNVNIGNL
810 820 830 840 850
RKLGNFTKPE MKVNFLKPNL KVNLWKSDSS LETMENPGVM GNKVQGESDG
860 870 880 890 900
DISSDNDSYH SDEFLTNSKS EEDKQLANSL ESVGPIDYIL PSCGIIVSAP
910 920 930 940 950
RLGSRSQSAS SIDVSTHAPS EAAAGPGSEL GKGLESPLKK SPSADSIHTR
960 970 980 990 1000
TGFTKPMDVY CQRFVQDAQN KMNDLSEIRS VAQKSEEGSH KTNRVSNEET
1010 1020 1030 1040 1050
QSEPMGQTPP RPSQLNVSCS VAGPPFLSVE PVHSVLSQKT PSSGSSLLEL
1060 1070 1080 1090 1100
EAGLCVTPSS ESSSSRAVSP FAKIRSSMVQ VANITQAGLT HGINLAVAKV
1110 1120 1130
QKSPAEPEAV NEIQQNELKN MFTQCQTRII QI
Length:1,132
Mass (Da):127,608
Last modified:March 1, 2003 - v1
Checksum:i4EDEA936E95D486C
GO
Isoform 2 (identifier: Q8CDA1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-627: Missing.
     628-629: PS → MH

Show »
Length:505
Mass (Da):55,241
Checksum:iD23C6ACE913BFB85
GO
Isoform 3 (identifier: Q8CDA1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-691: Missing.

Show »
Length:441
Mass (Da):47,885
Checksum:i4C855E8EBE10F926
GO
Isoform 4 (identifier: Q8CDA1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     373-414: VIVNLVDQAG...YVSFDFHEHC → RIWVWSQHPL...REEKRREEVT
     415-1132: Missing.

Show »
Length:414
Mass (Da):48,995
Checksum:i851CDFAF785F1EF6
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0J9YU13A0A0J9YU13_MOUSE
Phosphatidylinositide phosphatase S...
Inpp5f
121Annotation score:

Sequence cautioni

The sequence BAC28723 differs from that shown. Reason: Frameshift at position 692.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti728E → D in AAH67200 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0332701 – 691Missing in isoform 3. 1 PublicationAdd BLAST691
Alternative sequenceiVSP_0332711 – 627Missing in isoform 2. 1 PublicationAdd BLAST627
Alternative sequenceiVSP_033272373 – 414VIVNL…FHEHC → RIWVWSQHPLTQREEKRREE KRREEKRREEKRREEKRREE VT in isoform 4. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_033273415 – 1132Missing in isoform 4. 1 PublicationAdd BLAST718
Alternative sequenceiVSP_033274628 – 629PS → MH in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129249 mRNA Translation: BAC98059.1
AK030870 mRNA Translation: BAC27166.1
AK034482 mRNA Translation: BAC28723.1 Frameshift.
AK047166 mRNA Translation: BAC32978.1
AK150418 mRNA Translation: BAE29542.1
BC067200 mRNA Translation: AAH67200.1
BC125437 mRNA Translation: AAI25438.1
CCDSiCCDS21899.1 [Q8CDA1-1]
CCDS85430.1 [Q8CDA1-2]
RefSeqiNP_001333446.1, NM_001346517.1 [Q8CDA1-2]
NP_848756.2, NM_178641.5 [Q8CDA1-1]
UniGeneiMm.490344

Genome annotation databases

EnsembliENSMUST00000043138; ENSMUSP00000045910; ENSMUSG00000042105 [Q8CDA1-1]
ENSMUST00000118605; ENSMUSP00000113700; ENSMUSG00000042105 [Q8CDA1-2]
ENSMUST00000151237; ENSMUSP00000146197; ENSMUSG00000042105 [Q8CDA1-3]
GeneIDi101490
KEGGimmu:101490
UCSCiuc009jzc.1 mouse [Q8CDA1-1]
uc009jzf.1 mouse [Q8CDA1-3]
uc009jzg.1 mouse [Q8CDA1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129249 mRNA Translation: BAC98059.1
AK030870 mRNA Translation: BAC27166.1
AK034482 mRNA Translation: BAC28723.1 Frameshift.
AK047166 mRNA Translation: BAC32978.1
AK150418 mRNA Translation: BAE29542.1
BC067200 mRNA Translation: AAH67200.1
BC125437 mRNA Translation: AAI25438.1
CCDSiCCDS21899.1 [Q8CDA1-1]
CCDS85430.1 [Q8CDA1-2]
RefSeqiNP_001333446.1, NM_001346517.1 [Q8CDA1-2]
NP_848756.2, NM_178641.5 [Q8CDA1-1]
UniGeneiMm.490344

3D structure databases

ProteinModelPortaliQ8CDA1
SMRiQ8CDA1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045910

PTM databases

iPTMnetiQ8CDA1
PhosphoSitePlusiQ8CDA1

Proteomic databases

PaxDbiQ8CDA1
PeptideAtlasiQ8CDA1
PRIDEiQ8CDA1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043138; ENSMUSP00000045910; ENSMUSG00000042105 [Q8CDA1-1]
ENSMUST00000118605; ENSMUSP00000113700; ENSMUSG00000042105 [Q8CDA1-2]
ENSMUST00000151237; ENSMUSP00000146197; ENSMUSG00000042105 [Q8CDA1-3]
GeneIDi101490
KEGGimmu:101490
UCSCiuc009jzc.1 mouse [Q8CDA1-1]
uc009jzf.1 mouse [Q8CDA1-3]
uc009jzg.1 mouse [Q8CDA1-2]

Organism-specific databases

CTDi22876
MGIiMGI:2141867 Inpp5f
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1890 Eukaryota
COG5329 LUCA
GeneTreeiENSGT00530000063393
HOGENOMiHOG000046510
HOVERGENiHBG095361
InParanoidiQ8CDA1
KOiK21798
OMAiAYCHQFV
OrthoDBiEOG091G059J
PhylomeDBiQ8CDA1
TreeFamiTF313543

Enzyme and pathway databases

ReactomeiR-MMU-1660516 Synthesis of PIPs at the early endosome membrane

Miscellaneous databases

PROiPR:Q8CDA1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042105 Expressed in 251 organ(s), highest expression level in central nervous system
ExpressionAtlasiQ8CDA1 baseline and differential
GenevisibleiQ8CDA1 MM

Family and domain databases

InterProiView protein in InterPro
IPR034753 hSac2
IPR022158 Inositol_phosphatase
IPR002013 SAC_dom
PfamiView protein in Pfam
PF12456 hSac2, 1 hit
PF02383 Syja_N, 1 hit
PROSITEiView protein in PROSITE
PS51791 HSAC2, 1 hit
PS50275 SAC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSAC2_MOUSE
AccessioniPrimary (citable) accession number: Q8CDA1
Secondary accession number(s): Q3UCS0
, Q6NX83, Q6ZQ16, Q8C8G7, Q8CBW2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2003
Last modified: November 7, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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