UniProtKB - Q8CD15 (RIOX2_MOUSE)
Protein
Ribosomal oxygenase 2
Gene
Riox2
Organism
Mus musculus (Mouse)
Status
Functioni
Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39' (By similarity). May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.By similarity1 Publication
Catalytic activityi
Cofactori
Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 179 | Iron; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 181 | Iron; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 240 | Iron; catalyticPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 2-oxoglutarate-dependent dioxygenase activity Source: GO_Central
- histone demethylase activity (H3-K36 specific) Source: GO_Central
- histone demethylase activity (H3-K4 specific) Source: GO_Central
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- transcription corepressor activity Source: MGI
GO - Biological processi
- cell population proliferation Source: MGI
- histone H3-K36 demethylation Source: GO_Central
- histone H3-K4 demethylation Source: GO_Central
- regulation of cell population proliferation Source: MGI
- ribosome biogenesis Source: UniProtKB-KW
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Ribosome biogenesis, Transcription, Transcription regulation |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-3214842, HDMs demethylate histones |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal oxygenase 2ImportedAlternative name(s): Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA (EC:1.14.11.-) Histone lysine demethylase MINA MYC-induced nuclear antigen |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914264, Riox2 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- nucleolus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000308378 | 1 – 465 | Ribosomal oxygenase 2Add BLAST | 465 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 309 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8CD15 |
MaxQBi | Q8CD15 |
PaxDbi | Q8CD15 |
PeptideAtlasi | Q8CD15 |
PRIDEi | Q8CD15 |
PTM databases
iPTMneti | Q8CD15 |
PhosphoSitePlusi | Q8CD15 |
Expressioni
Tissue specificityi
Predominantly expressed in testis. Expressed at high levels in spleen, thymus, and colon, but barely detectable in brain, skeletal muscle, and seminal vesicle (at protein level).1 Publication
Developmental stagei
In testis, expressed in the nuclei of spermatogonia at all stages of the seminiferous epithelial cycle, and in meiotic prophase cells such as preleptotene, leptotene and zygotene, and weakly in early pachytene spermatocytes, but is absent in late pachytene spermatocytes, spermatids and mature sperm (at protein level).1 Publication
Inductioni
Up-regulated in experimentally-induced cryptorchid testis.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000022724, Expressed in ear and 304 other tissues |
ExpressionAtlasi | Q8CD15, baseline and differential |
Genevisiblei | Q8CD15, MM |
Interactioni
GO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
IntActi | Q8CD15, 1 interactor |
MINTi | Q8CD15 |
STRINGi | 10090.ENSMUSP00000023407 |
Miscellaneous databases
RNActi | Q8CD15, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 139 – 271 | JmjCPROSITE-ProRule annotationAdd BLAST | 133 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3706, Eukaryota |
GeneTreei | ENSGT00390000000083 |
HOGENOMi | CLU_013645_0_1_1 |
InParanoidi | Q8CD15 |
OMAi | RPTHEFT |
OrthoDBi | 693909at2759 |
PhylomeDBi | Q8CD15 |
TreeFami | TF318659 |
Family and domain databases
InterProi | View protein in InterPro IPR003347, JmjC_dom IPR039994, JmjC_protein |
PANTHERi | PTHR13096, PTHR13096, 1 hit |
Pfami | View protein in Pfam PF08007, Cupin_4, 1 hit |
PROSITEi | View protein in PROSITE PS51184, JMJC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Q8CD15-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPKKVQPTGD ENEEASVPCK RVKEELPETL SVLNFDSPSS FFESLISPIK
60 70 80 90 100
VETFFKEFWE QKPLLIQRDD PVLAKYYQSL FSLSDLKRLC KKGVYYGRDV
110 120 130 140 150
NVCRSISGKK KVLNKDGRAH FLQLRKDFDQ KRATIQFHQP QRYKDELWRI
160 170 180 190 200
QEKLECYFGS LVGSNVYMTP AGSQGLPPHY DDVEVFILQL EGTKHWRLYS
210 220 230 240 250
PTVPLAHEYS VESEDRIGTP THDFLLKPGD LLYFPRGTIH QAETPSGLAY
260 270 280 290 300
SIHLTISTYQ NNSWGDCLLD SISGFVFDIA KEDVALRSGM PRRMLLNVET
310 320 330 340 350
PADVTRKLSG FLRTLADQLE GREELLSSDM KKDFVKHRLP PFFEGNGTET
360 370 380 390 400
MDPGKQLPRL DNIIRLQFKD HIVLTVGPDK NPFDEAQQKV VYIYHSLKNV
410 420 430 440 450
RQMHMIGEEE ESEIFGLRFP LSHVDALKQI WCGSPIRVKD LKLDTDEEKE
460
NLALSLWSES LIQVL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YTU3 | D3YTU3_MOUSE | Bifunctional lysine-specific demeth... | Riox2 Mina | 373 | Annotation score: | ||
D3Z287 | D3Z287_MOUSE | Bifunctional lysine-specific demeth... | Riox2 Mina | 73 | Annotation score: | ||
Q8CCK9 | Q8CCK9_MOUSE | Bifunctional lysine-specific demeth... | Riox2 Mina | 156 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 | A → E in AAH25109 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 15 | A → E in AAH23462 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 107 | S → N in BAC27503 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 440 | D → E in AAH25109 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 440 | D → E in AAH23462 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB177385 mRNA Translation: BAD60965.1 AK007810 mRNA Translation: BAB25275.1 AK013451 mRNA Translation: BAB28860.1 AK031671 mRNA Translation: BAC27503.1 BC023462 mRNA Translation: AAH23462.1 BC025109 mRNA Translation: AAH25109.1 BC058242 mRNA Translation: AAH58242.1 |
CCDSi | CCDS28257.1 |
RefSeqi | NP_080186.3, NM_025910.3 XP_006522542.1, XM_006522479.3 |
Genome annotation databases
Ensembli | ENSMUST00000023407; ENSMUSP00000023407; ENSMUSG00000022724 ENSMUST00000160571; ENSMUSP00000125297; ENSMUSG00000022724 |
GeneIDi | 67014 |
KEGGi | mmu:67014 |
UCSCi | uc007zph.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB177385 mRNA Translation: BAD60965.1 AK007810 mRNA Translation: BAB25275.1 AK013451 mRNA Translation: BAB28860.1 AK031671 mRNA Translation: BAC27503.1 BC023462 mRNA Translation: AAH23462.1 BC025109 mRNA Translation: AAH25109.1 BC058242 mRNA Translation: AAH58242.1 |
CCDSi | CCDS28257.1 |
RefSeqi | NP_080186.3, NM_025910.3 XP_006522542.1, XM_006522479.3 |
3D structure databases
SMRi | Q8CD15 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q8CD15, 1 interactor |
MINTi | Q8CD15 |
STRINGi | 10090.ENSMUSP00000023407 |
PTM databases
iPTMneti | Q8CD15 |
PhosphoSitePlusi | Q8CD15 |
Proteomic databases
EPDi | Q8CD15 |
MaxQBi | Q8CD15 |
PaxDbi | Q8CD15 |
PeptideAtlasi | Q8CD15 |
PRIDEi | Q8CD15 |
Protocols and materials databases
Antibodypediai | 2005, 370 antibodies |
DNASUi | 67014 |
Genome annotation databases
Ensembli | ENSMUST00000023407; ENSMUSP00000023407; ENSMUSG00000022724 ENSMUST00000160571; ENSMUSP00000125297; ENSMUSG00000022724 |
GeneIDi | 67014 |
KEGGi | mmu:67014 |
UCSCi | uc007zph.2, mouse |
Organism-specific databases
CTDi | 84864 |
MGIi | MGI:1914264, Riox2 |
Phylogenomic databases
eggNOGi | KOG3706, Eukaryota |
GeneTreei | ENSGT00390000000083 |
HOGENOMi | CLU_013645_0_1_1 |
InParanoidi | Q8CD15 |
OMAi | RPTHEFT |
OrthoDBi | 693909at2759 |
PhylomeDBi | Q8CD15 |
TreeFami | TF318659 |
Enzyme and pathway databases
Reactomei | R-MMU-3214842, HDMs demethylate histones |
Miscellaneous databases
BioGRID-ORCSi | 67014, 1 hit in 19 CRISPR screens |
ChiTaRSi | Mina, mouse |
PROi | PR:Q8CD15 |
RNActi | Q8CD15, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022724, Expressed in ear and 304 other tissues |
ExpressionAtlasi | Q8CD15, baseline and differential |
Genevisiblei | Q8CD15, MM |
Family and domain databases
InterProi | View protein in InterPro IPR003347, JmjC_dom IPR039994, JmjC_protein |
PANTHERi | PTHR13096, PTHR13096, 1 hit |
Pfami | View protein in Pfam PF08007, Cupin_4, 1 hit |
PROSITEi | View protein in PROSITE PS51184, JMJC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RIOX2_MOUSE | |
Accessioni | Q8CD15Primary (citable) accession number: Q8CD15 Secondary accession number(s): Q8QZX1, Q9CQ03 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 23, 2007 |
Last sequence update: | October 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 126 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families