UniProtKB - Q8CCS6 (PABP2_MOUSE)
Polyadenylate-binding protein 2
Pabpn1
Functioni
GO - Molecular functioni
- poly(A) binding Source: MGI
- protein self-association Source: MGI
- RNA polymerase binding Source: UniProtKB
GO - Biological processi
- cellular response to lipopolysaccharide Source: UniProtKB
- MAPK cascade Source: UniProtKB
- mRNA polyadenylation Source: MGI
- poly(A)+ mRNA export from nucleus Source: MGI
- positive regulation of polynucleotide adenylyltransferase activity Source: UniProtKB
Keywordsi
Molecular function | RNA-binding |
Biological process | mRNA processing |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway R-MMU-72187, mRNA 3'-end processing R-MMU-73856, RNA Polymerase II Transcription Termination R-MMU-77595, Processing of Intronless Pre-mRNAs |
Names & Taxonomyi
Protein namesi | Recommended name: Polyadenylate-binding protein 2Short name: PABP-2 Short name: Poly(A)-binding protein 2 Alternative name(s): Nuclear poly(A)-binding protein 1 Poly(A)-binding protein II Short name: PABII Polyadenylate-binding nuclear protein 1 |
Gene namesi | Name:Pabpn1 Synonyms:Pab2, Pabp2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1859158, Pabpn1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
- Nucleus speckle By similarity
Other locations
- Cytoplasm By similarity
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus. Its nuclear import may involve the nucleocytoplasmic transport receptor transportin and a RAN-GTP-sensitive import mechanism. It is exported to the cytoplasm by a carrier-mediated pathway that is independent of mRNA traffic. Nucleus; nuclear speckle (By similarity). Colocalizes with SKIP and poly(A) RNA in nuclear speckles (By similarity).By similarity
Nucleus
- nuclear inclusion body Source: UniProtKB
- nuclear speck Source: UniProtKB-SubCell
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB-SubCell
- ribonucleoprotein complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000081712 | 2 – 302 | Polyadenylate-binding protein 2Add BLAST | 301 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 17 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 19 | PhosphoserineBy similarity | 1 | |
Modified residuei | 52 | PhosphoserineBy similarity | 1 | |
Modified residuei | 91 | PhosphoserineBy similarity | 1 | |
Modified residuei | 146 | PhosphoserineBy similarity | 1 | |
Modified residuei | 231 | PhosphoserineBy similarity | 1 | |
Modified residuei | 234 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 234 | Omega-N-methylarginine; alternateBy similarity | 1 | |
Modified residuei | 255 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 255 | Omega-N-methylarginine; alternateBy similarity | 1 | |
Modified residuei | 259 | Asymmetric dimethylarginine; alternateBy similarity | 1 | |
Modified residuei | 259 | Omega-N-methylarginine; alternateBy similarity | 1 | |
Modified residuei | 261 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 263 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 265 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 273 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 275 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 283 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 285 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 287 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 290 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 292 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 294 | Asymmetric dimethylarginineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
jPOSTi | Q8CCS6 |
PaxDbi | Q8CCS6 |
PRIDEi | Q8CCS6 |
PTM databases
iPTMneti | Q8CCS6 |
PhosphoSitePlusi | Q8CCS6 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000022194, Expressed in retinal neural layer and 307 other tissues |
ExpressionAtlasi | Q8CCS6, baseline and differential |
Genevisiblei | Q8CCS6, MM |
Interactioni
Subunit structurei
Monomer and homooligomer.
Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Binds RNA as a monomer and oligomerizes when bound to poly(A).
Interacts with PAPOLA, but only in presence of oligo(A) RNA.
Interacts with NUDT21/CPSF5 and transportin. Associates in a ternary complex with CPSF4 and NS/NS1 and interaction with NS/NS1, blocks nuclear export of host cell mRNAs. Associates in a single complex with SKIP and MYOD1 and interacts with SKIP in differentiated myocytes. May interact with SETX (By similarity).
Interacts (via RRM domain and C-terminal arginine-rich region) with ZFP36 (via hypophosphorylated form); this interaction occurs in the nucleus in a RNA-independent manner, decreases in presence of single-stranded poly(A) RNA-oligomer and in a p38-dependent-manner and may down-regulated RNA poly(A) polymerase activity (PubMed:22844456).
Component of the poly(A) tail exosome targeting (PAXT) complex composed of PABPN1, ZFC3H1 and MTREX.
Interacts with ZFC3H1 in a RNase-insensitive manner (By similarity).
By similarity1 PublicationGO - Molecular functioni
- protein self-association Source: MGI
- RNA polymerase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 207596, 23 interactors |
IntActi | Q8CCS6, 4 interactors |
MINTi | Q8CCS6 |
STRINGi | 10090.ENSMUSP00000022808 |
Miscellaneous databases
RNActi | Q8CCS6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 168 – 245 | RRMPROSITE-ProRule annotationAdd BLAST | 78 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 141 | Interaction with SKIPBy similarityAdd BLAST | 140 | |
Regioni | 115 – 143 | Stimulates PAPOLABy similarityAdd BLAST | 29 | |
Regioni | 255 – 302 | Strong poly(A) affinity and self-associationBy similarityAdd BLAST | 48 | |
Regioni | 282 – 302 | Interaction with PAPOLABy similarityAdd BLAST | 21 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 111 – 147 | Sequence analysisAdd BLAST | 37 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 2 – 14 | Ala-richAdd BLAST | 13 |
Domaini
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG4209, Eukaryota |
GeneTreei | ENSGT00940000154606 |
HOGENOMi | CLU_012062_23_1_1 |
InParanoidi | Q8CCS6 |
OrthoDBi | 1412946at2759 |
PhylomeDBi | Q8CCS6 |
TreeFami | TF105907 |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR034911, PABP2 IPR035979, RBD_domain_sf IPR000504, RRM_dom |
PANTHERi | PTHR23236:SF16, PTHR23236:SF16, 1 hit |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAAAAAAAA AGAAGGRGSG PGRRRHLVPG AGGEAGEGDP GGAGDYGNGL
60 70 80 90 100
ESEELEPGEL LPEPEPEEEP PRPRAPPGAP GPGPGSGAPG SQEEEEEPGL
110 120 130 140 150
VEADPGDGAI EDPELEAIKA RVREMEEEAE KLKELQNEVE KQMNMSPPPG
160 170 180 190 200
NAGPVIMSLE EKMEADARSI YVGNVDYGAT AEELEAHFHG CGSVNRVTIL
210 220 230 240 250
CDKFSGHPKG FAYIEFSDKE SVRTSLALDE SLFRGRQIKV IPKRTNRPGI
260 270 280 290 300
STTDRGFPRS RYRARTTNYN SSRSRFYSGF NSRPRGRIYR GRARATSWYS
PY
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3UY42 | G3UY42_MOUSE | Polyadenylate-binding protein 2 | Pabpn1 | 319 | Annotation score: | ||
D3YUB9 | D3YUB9_MOUSE | Polyadenylate-binding protein 2 | Pabpn1 | 164 | Annotation score: | ||
G3UWS5 | G3UWS5_MOUSE | Polyadenylate-binding protein 2 | Pabpn1 | 178 | Annotation score: | ||
D3Z055 | D3Z055_MOUSE | Polyadenylate-binding protein 2 | Pabpn1 | 168 | Annotation score: | ||
G3UX74 | G3UX74_MOUSE | Polyadenylate-binding protein 2 | Pabpn1 | 73 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009849 | 291 – 292 | GR → SG in isoform 2. 1 Publication | 2 | |
Alternative sequenceiVSP_009850 | 293 – 302 | Missing in isoform 2. 1 Publication | 10 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U93050 Genomic DNA Translation: AAC00210.1 AK032172 mRNA Translation: BAC27741.1 BC055866 mRNA Translation: AAH55866.1 |
CCDSi | CCDS27104.1 [Q8CCS6-1] |
RefSeqi | NP_062275.1, NM_019402.2 [Q8CCS6-1] |
Genome annotation databases
Ensembli | ENSMUST00000022808; ENSMUSP00000022808; ENSMUSG00000022194 [Q8CCS6-1] ENSMUST00000116476; ENSMUSP00000112177; ENSMUSG00000022194 [Q8CCS6-2] |
GeneIDi | 54196 |
KEGGi | mmu:54196 |
UCSCi | uc007txg.2, mouse [Q8CCS6-1] uc007txi.2, mouse [Q8CCS6-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U93050 Genomic DNA Translation: AAC00210.1 AK032172 mRNA Translation: BAC27741.1 BC055866 mRNA Translation: AAH55866.1 |
CCDSi | CCDS27104.1 [Q8CCS6-1] |
RefSeqi | NP_062275.1, NM_019402.2 [Q8CCS6-1] |
3D structure databases
SMRi | Q8CCS6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 207596, 23 interactors |
IntActi | Q8CCS6, 4 interactors |
MINTi | Q8CCS6 |
STRINGi | 10090.ENSMUSP00000022808 |
PTM databases
iPTMneti | Q8CCS6 |
PhosphoSitePlusi | Q8CCS6 |
Proteomic databases
jPOSTi | Q8CCS6 |
PaxDbi | Q8CCS6 |
PRIDEi | Q8CCS6 |
Protocols and materials databases
Antibodypediai | 43, 168 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000022808; ENSMUSP00000022808; ENSMUSG00000022194 [Q8CCS6-1] ENSMUST00000116476; ENSMUSP00000112177; ENSMUSG00000022194 [Q8CCS6-2] |
GeneIDi | 54196 |
KEGGi | mmu:54196 |
UCSCi | uc007txg.2, mouse [Q8CCS6-1] uc007txi.2, mouse [Q8CCS6-2] |
Organism-specific databases
CTDi | 8106 |
MGIi | MGI:1859158, Pabpn1 |
Phylogenomic databases
eggNOGi | KOG4209, Eukaryota |
GeneTreei | ENSGT00940000154606 |
HOGENOMi | CLU_012062_23_1_1 |
InParanoidi | Q8CCS6 |
OrthoDBi | 1412946at2759 |
PhylomeDBi | Q8CCS6 |
TreeFami | TF105907 |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway R-MMU-72187, mRNA 3'-end processing R-MMU-73856, RNA Polymerase II Transcription Termination R-MMU-77595, Processing of Intronless Pre-mRNAs |
Miscellaneous databases
BioGRID-ORCSi | 54196, 10 hits in 18 CRISPR screens |
ChiTaRSi | Pabpn1, mouse |
PROi | PR:Q8CCS6 |
RNActi | Q8CCS6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022194, Expressed in retinal neural layer and 307 other tissues |
ExpressionAtlasi | Q8CCS6, baseline and differential |
Genevisiblei | Q8CCS6, MM |
Family and domain databases
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR034911, PABP2 IPR035979, RBD_domain_sf IPR000504, RRM_dom |
PANTHERi | PTHR23236:SF16, PTHR23236:SF16, 1 hit |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PABP2_MOUSE | |
Accessioni | Q8CCS6Primary (citable) accession number: Q8CCS6 Secondary accession number(s): O35935 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 143 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot